Directing differential cellular response by manipulating the physical characteristics of the material is regarded as a key challenge in biomaterial implant design and tissue engineering. In developing various biomaterials, the influence of substrate properties, like surface topography, stiffness and wettability on the cell functionality has been investigated widely. However, such study to probe into the influence of substrate conductivity on cell fate processes is rather limited. The need for such an understanding is based on the fact that specific tissues in the body are electrically active in nature, such as in brain, heart and skeletal muscle. These tissues make use of electrical conductivity as an effective cue for tissue homeostasis, development, regeneration and so on. Moreover, understanding the importance of underlying conductivity in basic biological processes is essential in developing electrically conductive biomaterials with the ability to simulate normal electrophysiology of the body by interfacing with bioelectric fields in cells and tissues. Electrical stimulation and charge conduction can regulate numerous intracellular signalling pathways, can interact with cytoskeleton proteins to modulate the morphology, increase protein synthesis and on the more can favor the ECM protein conformational changes. On these grounds, the present dissertation illustrates that persistent electrical activation influences the multipotency of hMSCs and acts like a promoter towards selective differentiation of hMSCs into neural/cardiomyogenic or osteogenic lineage. Besides, continual exposure to electric field stimulated conducting culture environments lead to growth arrest while enhancing differentiation. In total, this dissertation suggests the dominant role of conductivity in inducing my oblast differentiation and hMSc lineage commitment that involves EF stimulated in vitro culture conditions. Also, a knowledge base with qualitative and quantitative understanding of stem cells and their response to substrate physical properties and external field effect was developed through this comprehensive study. Such an improved understanding of the ability of hMSCs in sensing electrical conductivity may lead to the development of culture additives/conditions that better induce directed stem cell differentiation.

For biomedical electronics the compatibility to the biological environment should be well-considered. Therefore this paper evaluates dispensed carbon nanotubes (CNT's) on polyimide (PI) foil for conductive tracks and electrodes for flexible, biomedical applications. A CNT based ink is investigated regarding biocompatibility, flexibility, conductivity and suitability for electrode materials with contact to artificial body fluids. The testing methods comprise bending tests with resistance monitoring, adhesion tests and the utilization of dynamic fluidic and electrical load on dispensed structures. The CNT ink showed good bending properties up to 2653 cycles with an average sheet resistance of 32.5 Ohm/sq. A demonstration of biocompatibility using the adherent cell line HFFF2 resulted negatively. No delamination or dissolving effects occurred during exposure to 0.9 % sodium chloride solution.

No description available.

No description available.

Background: Despite our distinct impression to the contrary, we are only conscious of a fraction of all the neural activity underlying our thoughts and behavior. Most neural processes occur non-consciously, and in parallel with our conscious experience. However, it is still unclear what the limits of non-conscious processes are in terms of higher cognitive functions. Many recent studies have shown that increasingly more advanced functions can operate non-consciously, but non-conscious information is still thought to be fleeting and undetectable within 500 milliseconds. Here we used various techniques to render information non-conscious, together with functional magnetic resonance imaging (fMRI), to investigate if non-consciously presented information can be retained for several seconds, what the neural substrates of such retention are, and if it is consistent with working memory maintenance. Results: In Study I we used an attentional blink paradigm to render stimuli (single letters) non-conscious, and a variable delay period (5 – 15 s) prior to memory test. It was found that non-conscious memory performance was above chance after all delay durations, and showed no signs of decline over time. Univariate fMRI analysis showed that the durable retention was associated with sustained BOLD signal change in the prefrontal cortex and cerebellum during the delay period. In Study II we used continuous flash suppression (CFS) to render stimuli (faces and tools) non-conscious, and a variable delay period (5 or 15 s) prior to memory test. The durable retention of up to 15 s was replicated, and it was found that stimuli identity and spatial position was retained until prospective use. In Study III we used CFS to render tools non-conscious, and a variable delay period (5 – 15 s) prior to memory test. It was found that memory performance was not better than chance. However, by using multi-voxel pattern analysis it was nonetheless possible to detect the presence vs. absence of non-conscious stimuli in the frontal cortex,and their spatial position (left vs. right) in the occipital cortex during the delay. Conclusions: Overall these findings suggest that non-consciously presented information (identity and/or position) can be retained for several seconds,and is associated with BOLD signal in frontal and posterior regions. These findings are consistent with working memory maintenance of non-consciously presented information, and thereby constrain models of working memory and theories of consciousness.

Photoconductive antenna (PCA) array based THz near-field imager incorporating Hadamard multiplexing method is developed in this work. By using a 2 × 2 dipole antenna array as the THz transmitter, the system signal-to-noise ratio (SNR) is demonstrated to be improved by a factor of 2 as the theory predicts. Additionally, a 2-D scanning of a metallic structure on a THz-transparent substrate (with a total scanning area of 1 × 1 mm2) is experimentally implemented. Correlation coefficient estimation is made afterwards to quantify the reconstructed image quality.

Os surfatantes, moléculas anfipáticas compostas por uma porção polar e outra apolar, constituem um grupo heterogêneo de compostos de superfície ativa. Sua porção polar pode ser formada por peptídeos, ânions ou cátions, por mono, di ou polissacarídeos, enquanto sua porção apolar pode ser formada por estruturas saturadas, insaturadas ou ácidos graxos hidroxilados, ou ainda peptídeos hidrofóbicos. Essas substâncias podem ser sintéticas, obtidas a partir de síntese química, ou produzidas por micro-organismos, principalmente, bactérias e leveduras, passando a ser denominadas biossurfatantes (BS). O BS representa uma alternativa aos tensoativos sintéticos utilizados em diversos seguimentos da indústria devido a sua baixa toxicidade e alta biodegradabilidade, além de aplicabilidade na descontaminação ambiental. Bactérias isoladas em diversos biomas têm sido muito exploradas para produção de BS, enquanto ecossistemas marinhos ainda são pouco explorados, apesar do grande potencial existente. Os micro-organismos marinhos quando expostos a condições extremas de pressão, salinidade e temperatura produzem compostos estáveis e, portanto, úteis em aplicações industriais. O presente trabalho objetivou investigar novos BS produzidos por bactérias de origem marinha capazes de produzir tal substância a partir de fontes de carbono de baixo custo (glicerol, óleo de soja, vaselina e sacarose). Foram analisados 59 isolados bacterianos marinhos, as bactérias selecionadas foram identificadas e a produção do BS foi estudada em escala laboratorial. Após a extração do BS, suas propriedades físico-química como tensão superficial (TS), tensão interfacial (IT) e concentração micelar crítica (CMC) foram determinadas; além disso, foi realizada a caracterização estrutural preliminar e o seu potencial de aplicação para biorremediação de petróleo e como agente emulsificador. Os resultados identificaram a produção de BS por três bactérias marinhas: Arthrobacter defluvii, Brevibacterium luteolum e Gordonia sp. A partir das análises químicas foi possível identificar dois BS lipopepítidicos (A. Defluvii e B. luteolum) e um BS com grupos glicosídicos (Gordonia sp.). O BS produzido por A. defluvii apresentou tensão superficial TS = 34,5 mN m-1; TI = 15 mN m-1; e CMC = 129 mg L-1 crescendo em óleo de soja, B. luteolum TS = 27 mN m-1; TI = 0,84 mN m-1; e CMC = 40 mg L-1 crescendo em vaselina e Gordonia sp. TS = 33 mN m-1; TI = 1,4 mN m-1; e CMC = 85 mg L-1 crescendo em óleo de soja. Os BS estudados apresentaram capacidade de remoção de petróleo de areia contaminada, assim como atividade emulsificante frente a diferentes substâncias ( óleo de soja, vaselina, decano, querosene, hexadecano e gordura vegetal). Os testes de estabilidade realizados para BS produzido por B. luteolum indicaram que: o BS é estável quando submetido a temperaturas até 60oC por 24 h e até 121oC por 20 min; a maior atividade tensoativa para o BS é encontrada em solução com pH entre 6 e 8; e a força iônica não afeta a atividade tensoativa até concentrações salinas abaixo de 16%. Portanto, os três micro-organismos marinhos selecionados foram capazes de produzir compostos com relevante atividade tensoativa utilizando substratos de baixo custo. / The surfactants, amphipathic molecules containing both polar and nonpolar portions, are a heterogeneous group of surface-active compounds. Its polar portion may be composed by peptides, anions or cations, mono, di, or polysaccharides, while its polar portion may contain saturated, unsaturated or hydroxylated fatty acids or hydrophobic peptides. These molecules may be synthetic, derived from chemical synthesis, or produced by microorganisms, mainly bacteria and yeast, and also known as biosurfactants (BS). The BS represent an alternative to synthetic surfactants used in various segments of industry due to their low toxicity and high biodegradability. The major classes of BS include glycolipids, lipopeptides, lipoproteins, phospholipids, fatty acids and polymeric surfactants. Bacteria isolated from different biomes have been heavily exploited for BS production, while marine ecosystems are still poorly explored, despite its great potential. The marine microorganisms when exposed to extremes of pressure, temperature and salinity, produce stable compounds and, therefore, useful in industrial applications. The aim of this study was to investigate new BS produced by marine bacteria capable of producing such molecules growing in low-cost carbon sources (mineral oil, sucrose, soybean oil and glycerol). The selected bacterial isolates were identified and the biosurfactant production was studied in laboratory scale. After extraction of BS their physicochemical properties as surface tension (ST), interfacial tension (IT) and critical micelle concentration (CMC) were determined; preliminary structural characterization evaluated by FTIR and TLC and their potential application in bioremediation of crude oil and as emulsifier was also investigated. The results identified the BS production by three marine bacteria Arthrobacter defluvii, Brevibacterium luteolum and Gordonia sp. Based on the chemical analysis it was possible to identify two lipopeptides BS (A. defluvii and B. luteolum) and a BS with glycosides groups (Gordonia sp.). The BS produced by A. defluvii growing in soybean oil showed ST = 34,5 mNm-1; IT = 15 mNm-1 and CMC = 129 mgL-1; B. luteolum growing in mineral oil showed a ST = 27 mNm-1, IT = 0,84 mNm-1 and CMC = 40 mgL-1 and Gordonia sp. growing in soybean oil showed ST = 33 mNm-1, IT = 1,4 mNm-1 and CMC = 85 mgL-1. The BS obtained exhibited capacity to remove crude oil from contaminated sand as well as emulsifying activity against different hydrophobic substances (soybean oil, mineral oil, decane, kerosene, animal fat and hexadecane). Stability tests carried out to BS produced by B. luteolum indicated that: the BS is stable when submitted to temperatures down to 60° C for 24 h to 121° C for 20 min; the higher activity to the BS is found in solution with pH between 6 and 8; and the ionic strength does not affect the surfactant activity until salt concentrations under 16%. Therefore, the three selected marine micro-organisms were able to produce compounds with significant surfactant activity using low cost substrates.

No território brasileiro existe uma grande quantidade de plantas frutíferas com potencial de comercialização, por causa de suas propriedades medicinais, como elevados teores de vitamina C e suas propriedades adstringentes. Frutíferas como as da família mirtácea: cabeludinha (Plinia glomerata Berg.), grumixama (Eugenia brasiliensis Lam.), surinam cherry (Eugenia uniflora), uvaia (Eugenia pyriformis Cambess) and camu-camu (Myrciaria dubia (H.B.K.) McVaugh), e também a lichia (Litchi chinensis Sonn.) da família sapindácea, são candidatas a se tornarem uma cultura economicamente importante, porém é necessário melhorar as condições para o desenvolvimento destas plantas. O aumento no uso do substrato, incluindo uma ampla variedade de materiais em sua formulação, favorecem as condições físicas, biológicas e químicas do meio , para um ótimo desenvolvimento. Dessa maneira, o objetivo deste trabalho foi avaliar a influência de diferentes misturas do substrato de casca de pínus, no desenvolvimento de mudas de plantas frutíferas. Utilizou-se o delineamento estatístico em blocos inteiramente ao acaso, com medidas repetidas no tempo, onde cada bloco (localização das bandejas de poliestireno expandido na estufa) era composto de 72 células, com 06 tratamentos (T1 = 100% material original (casca de pínus); T2 = 100% casca de pínus ≤0,1mm (partículas pequenas); T3 = 75% casca de pínus ≤0,1mm + 25% casca de pínus 0,1 - 4,0mm (partículas grandes); T4 = 50% casca de pínus ≤0,1mm + 50% casca de pínus 0,1 - 4,0mm; T5 = 25% casca de pínus ≤0,1mm + 75% casca de pínus 0,1 - 4,0mm; T6 = 100% casca de pínus 0,1-0,4mm) e 12 sementes em cada unidade experimental e com 5 repetições, perfazendo um total de 360 plantas, para cada espécie. Foram realizadas 3 avaliações para cada espécie utilizada, sendo a primeira realizada após três meses da semeadura, seguidas de duas avaliações mensais. Os dados obtidos foram analisados pelo programa estatístico SAS (2003). O aumento na proporção de partículas pequenas nos tratamentos diminui a taxa de germinação das sementes, pois a textura dessa classe granulométrica de substrato dificulta a absorção de água nos primeiros dias após a semeadura e prejudica a aeração para as raízes, após a quebra da tensão superficial. Este experimento mostrou que os substratos influem diretamente no desenvolvimento de mudas de plantas frutíferas. / In the Brazilian territory there are a great amount of fruti plants with commercialization potential because of their properties for use as medicinal plants, presenting high vitamin C content and astringent properties. Fruit plants such as those from myrtaceae family: cabeludinha (Plinia glomerata Berg.), grumixama (Eugenia brasiliensis Lam.), surinam cherry (Eugenia uniflora), uvaia (Eugenia pyriformis Cambess) and camu-camu (Myrciaria dubia (H.B.K.) McVaugh), and also the lichia (Litchi chinensis Sonn.) from sapindaceae family, are candidates to become economically important cultures, however, there is need for development of optimal conditions for development of those plants. An increment of the substrate usage, including a wide variety of materials in its formulation, has resulted in conditions that favor physical, chemical and biological means for optimal plant development. Therefore, the objective of this study was evaluate, the influence of the different mixture of the substrate made of pinus peel, in the development of seedlings of fruit plants. It was used the completely randomized blocks design, with repeated measures in the time, where each block (location of trays of expanded polystyrene in the greenhouse) was composed of 72 cells, with 06 treatments (T1 = 100% original material of pinus peel); T2 = 100% pinus peel ≤0,1mm (small particles)); T3 = 75% pinus peel ≤0,1mm + 25% pinus peel 0,1 - 4,0mm (big particles); T4 = 50% pinus peel ≤0,1mm + 50% pinus peel 0,1 - 4,0mm; T5 = 25% pinus peel ≤0,1mm + 75% pinus peel 0,1 - 4,0mm; T6 = 100% pinus peel 0,1- 0,4mm) and 12 seeds in each experimental unit, with 5 repetitions, doing a total of 360 plants, of each species. Three evaluations were accomplished for each species, being the first accomplished being after three months of the sowing, followed by two monthly evaluations thereafter. The obtained data were analyzed by the SAS statistical program (2003). The increase in the proportion of small particles into the substrate reduces the germination rate, because the fine texture of pinus peel hinders root water absorption and aeration in the initial days after sowing, after the breakage of the superficial tension. This experiment showed that the substrate influence directly on the developing of seedlings of the fruit plants.

Os surfatantes, moléculas anfipáticas compostas por uma porção polar e outra apolar, constituem um grupo heterogêneo de compostos de superfície ativa. Sua porção polar pode ser formada por peptídeos, ânions ou cátions, por mono, di ou polissacarídeos, enquanto sua porção apolar pode ser formada por estruturas saturadas, insaturadas ou ácidos graxos hidroxilados, ou ainda peptídeos hidrofóbicos. Essas substâncias podem ser sintéticas, obtidas a partir de síntese química, ou produzidas por micro-organismos, principalmente, bactérias e leveduras, passando a ser denominadas biossurfatantes (BS). O BS representa uma alternativa aos tensoativos sintéticos utilizados em diversos seguimentos da indústria devido a sua baixa toxicidade e alta biodegradabilidade, além de aplicabilidade na descontaminação ambiental. Bactérias isoladas em diversos biomas têm sido muito exploradas para produção de BS, enquanto ecossistemas marinhos ainda são pouco explorados, apesar do grande potencial existente. Os micro-organismos marinhos quando expostos a condições extremas de pressão, salinidade e temperatura produzem compostos estáveis e, portanto, úteis em aplicações industriais. O presente trabalho objetivou investigar novos BS produzidos por bactérias de origem marinha capazes de produzir tal substância a partir de fontes de carbono de baixo custo (glicerol, óleo de soja, vaselina e sacarose). Foram analisados 59 isolados bacterianos marinhos, as bactérias selecionadas foram identificadas e a produção do BS foi estudada em escala laboratorial. Após a extração do BS, suas propriedades físico-química como tensão superficial (TS), tensão interfacial (IT) e concentração micelar crítica (CMC) foram determinadas; além disso, foi realizada a caracterização estrutural preliminar e o seu potencial de aplicação para biorremediação de petróleo e como agente emulsificador. Os resultados identificaram a produção de BS por três bactérias marinhas: Arthrobacter defluvii, Brevibacterium luteolum e Gordonia sp. A partir das análises químicas foi possível identificar dois BS lipopepítidicos (A. Defluvii e B. luteolum) e um BS com grupos glicosídicos (Gordonia sp.). O BS produzido por A. defluvii apresentou tensão superficial TS = 34,5 mN m-1; TI = 15 mN m-1; e CMC = 129 mg L-1 crescendo em óleo de soja, B. luteolum TS = 27 mN m-1; TI = 0,84 mN m-1; e CMC = 40 mg L-1 crescendo em vaselina e Gordonia sp. TS = 33 mN m-1; TI = 1,4 mN m-1; e CMC = 85 mg L-1 crescendo em óleo de soja. Os BS estudados apresentaram capacidade de remoção de petróleo de areia contaminada, assim como atividade emulsificante frente a diferentes substâncias ( óleo de soja, vaselina, decano, querosene, hexadecano e gordura vegetal). Os testes de estabilidade realizados para BS produzido por B. luteolum indicaram que: o BS é estável quando submetido a temperaturas até 60oC por 24 h e até 121oC por 20 min; a maior atividade tensoativa para o BS é encontrada em solução com pH entre 6 e 8; e a força iônica não afeta a atividade tensoativa até concentrações salinas abaixo de 16%. Portanto, os três micro-organismos marinhos selecionados foram capazes de produzir compostos com relevante atividade tensoativa utilizando substratos de baixo custo. / The surfactants, amphipathic molecules containing both polar and nonpolar portions, are a heterogeneous group of surface-active compounds. Its polar portion may be composed by peptides, anions or cations, mono, di, or polysaccharides, while its polar portion may contain saturated, unsaturated or hydroxylated fatty acids or hydrophobic peptides. These molecules may be synthetic, derived from chemical synthesis, or produced by microorganisms, mainly bacteria and yeast, and also known as biosurfactants (BS). The BS represent an alternative to synthetic surfactants used in various segments of industry due to their low toxicity and high biodegradability. The major classes of BS include glycolipids, lipopeptides, lipoproteins, phospholipids, fatty acids and polymeric surfactants. Bacteria isolated from different biomes have been heavily exploited for BS production, while marine ecosystems are still poorly explored, despite its great potential. The marine microorganisms when exposed to extremes of pressure, temperature and salinity, produce stable compounds and, therefore, useful in industrial applications. The aim of this study was to investigate new BS produced by marine bacteria capable of producing such molecules growing in low-cost carbon sources (mineral oil, sucrose, soybean oil and glycerol). The selected bacterial isolates were identified and the biosurfactant production was studied in laboratory scale. After extraction of BS their physicochemical properties as surface tension (ST), interfacial tension (IT) and critical micelle concentration (CMC) were determined; preliminary structural characterization evaluated by FTIR and TLC and their potential application in bioremediation of crude oil and as emulsifier was also investigated. The results identified the BS production by three marine bacteria Arthrobacter defluvii, Brevibacterium luteolum and Gordonia sp. Based on the chemical analysis it was possible to identify two lipopeptides BS (A. defluvii and B. luteolum) and a BS with glycosides groups (Gordonia sp.). The BS produced by A. defluvii growing in soybean oil showed ST = 34,5 mNm-1; IT = 15 mNm-1 and CMC = 129 mgL-1; B. luteolum growing in mineral oil showed a ST = 27 mNm-1, IT = 0,84 mNm-1 and CMC = 40 mgL-1 and Gordonia sp. growing in soybean oil showed ST = 33 mNm-1, IT = 1,4 mNm-1 and CMC = 85 mgL-1. The BS obtained exhibited capacity to remove crude oil from contaminated sand as well as emulsifying activity against different hydrophobic substances (soybean oil, mineral oil, decane, kerosene, animal fat and hexadecane). Stability tests carried out to BS produced by B. luteolum indicated that: the BS is stable when submitted to temperatures down to 60° C for 24 h to 121° C for 20 min; the higher activity to the BS is found in solution with pH between 6 and 8; and the ionic strength does not affect the surfactant activity until salt concentrations under 16%. Therefore, the three selected marine micro-organisms were able to produce compounds with significant surfactant activity using low cost substrates.

Submitted by Gabriela Lopes (gmachadolopesufpel@gmail.com) on 2017-07-05T14:18:10Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertação Daniela versão final corrigida.pdf: 2062827 bytes, checksum: eba55722673c478fcbfe4ea404a69852 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-07-11T21:06:23Z (GMT) No. of bitstreams: 2 Dissertação Daniela versão final corrigida.pdf: 2062827 bytes, checksum: eba55722673c478fcbfe4ea404a69852 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-07-11T21:06:23Z (GMT). No. of bitstreams: 2 Dissertação Daniela versão final corrigida.pdf: 2062827 bytes, checksum: eba55722673c478fcbfe4ea404a69852 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-02-20 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / As flores de corte, de maneira geral, são bastante suscetíveis às variações ambientais, sobretudo deficiência hídrica, temperaturas extremas, problemas do meio de cultivo, que podem resultar no desenvolvimento deficiente da produção final. O cultivo de flores de corte em substrato disposto em canais, com recirculação da solução nutritiva drenada, pode ser uma alternativa para a obtenção de elevado rendimento produtivo de hastes florais de qualidade, além de promover a otimização de recursos, materiais e uso racional da água. Neste sentido, torna-se interessante o estudo do efeito de substratos à base de casca de arroz, material abundante no sul do Brasil, sobre o crescimento, a qualidade e as respostas produtivas das plantas de gipsofila (Gypsophila paniculata) e lisiantos (Eustoma grandiflorum). Paralelamente, é importante a utilização de adequado manejo fitotécnico para que as plantas respondam positivamente ao sistema proposto. Desta forma, dois experimentos foram conduzidos em casa de vegetação na Universidade Federal de Pelotas, no município de Capão do Leão – RS no ano de 2015/2016. O experimento 1 teve como objetivo avaliar diferentes substratos à base de casca de arroz [casca de arroz carbonizada (CAC) e casca de arroz in natura (CAIN), empregadas isoladamente, e as misturas de CAC + substrato comercial orgânico S10 (Beifort ) (15%) e CAIN + S10 (15%)] e a época de poda (precoce e tardia) para a produção de gipsofila. Avaliaram-se o crescimento, a produção, a qualidade, a soma térmica, o consumo hídrico e a fenologia da cultura. Os resultados obtidos no experimento 1 indicaram que, para todas as variáveis analisadas, não houve interação significativa entre substrato e época de poda. Pode-se indicar os substratos de CAC 100% ou a mistura CAIN + S10 (15%) para o cultivo de gipsofila em canais. O substrato CAIN 100% resultou em menor crescimento da parte aérea, menor produção e inferior qualidade das hastes. Os substratos não afetaram a partição de massa seca entre flores e órgãos vegetativos e o equilíbrio entre o crescimento da parte aérea e das raízes. A poda tardia antecipou o ciclo da cultura, aumentou o crescimento das hastes e a produtividade de gipsofila, melhorando a qualidade. O período de maior soma térmica acumulada (STa) para a poda precoce é na fase fenológica de elongação e iniciação floral (III) e para a tardia na fase vegetativa (I). O maior consumo de água pelas plantas ocorreu na fase III, tanto para o acumulado, quanto para o consumo diário. O consumo médio total acumulado durante o ciclo foi de 7,05 litros planta-1 e o consumo médio diário foi de 0,309 litros planta dia-1 . Os substratos CAC+S10 e CAIN+S10 foram superiores na eficiência no uso da água (EUA) para a produção de massa fresca (MF) em relação aos materiais puros, e não afetaram a produção de massa seca (MS). Foi verificado que as plantas de gipsofila produziram em média 9,36 g de MS para cada litro de água consumido. No segundo experimento, o objetivo foi avaliar o crescimento e a qualidade das plantas de lisiantos, também, em diferentes substratos à base de casca de arroz [casca de arroz carbonizada (CAC); casca de arroz in natura (CAIN); CAC (70%) + substrato comercial orgânico S10 (Beifort ) (30%) e CAIN (70%) + S10 (Beifort ) (30%)]. Os substratos CAIN + S10 e CAC podem ser utilizados para a produção de lisiantos para flor de corte em sistema de cultivo em canais com recirculação da solução nutritiva, uma vez que ambos proporcionaram hastes com elevado padrão de qualidade, com destaque para o primeiro, que promoveu um maior crescimento das plantas. O uso de CAIN como material isolado para substrato prejudicou o crescimento das plantas e impediu o florescimento, modificando as relações de partição de massa seca entre os órgãos das plantas. Os resultados são decorrentes da melhor qualidade das características físicas e químicas do substrato CAIN+S10, em virtude da presença do composto orgânico, que aumentou a capacidade de retenção de água, e da alta porosidade da CAIN. Assim, constata-se que a gipsofila e o lisiantos adaptaram-se de forma adequada ao sistema de cultivo em canais preenchidos com substratos à base de casca de arroz, com recirculação do lixiviado. / Cut flowers, in general, are very susceptible to environmental variations, especially water stress, excessive temperatures, composition of the culture medium, among others, resulting in the deficient and discrepant development of the final production. The cultivation of cut flowers in growing troughs filled with substrates and the recirculation of the nutrient solution can be an alternative to obtain high flower stems productive yield and quality, besides the optimization of resources and rational use of water. In this sense, to study the effect of substrates based on rice husk, a high availability material in southern Brazil, on the gypsophila (Gypsophila paniculata) and lisianthus (Eustoma grandiflorum) plant growth, quality and yield is necessary. In parallel, it's important to use phytotechnical management careful so that plants respond adequately to the proposed system. Thus, two experiments were conducted in a greenhouse at the Federal University of Pelotas (UFPel), Capão do Leão city - RS, between 2015/2016. The experiment 1 had the objective of evaluating different substrates based on carbonized rice husk (CAC) and raw rice husk (CAIN), used in isolation, and the mixtures of CAC + commercial organic substrate S10 (Beifort®) (15%), CAIN + S10 (15%) and the pinch season (early and late) for the gipsofila production. Growth, production, quality, water consumption and crop phenology were evaluated. The results obtained in experiment 1 indicated that, for all variables analyzed, there was no significant interaction between substrate and pinch season. The substrates of 100% CAC or the CAIN + S10 mixture (15%) may be indicated for Gypsophila cultivation in troughs. The 100% CAIN substrate predisposed to lower shoot growth, lower shoot production and lower quality. However, the substrates did not affect the dry matter partitioning between flowers and vegetative organs and the balance between shoot and root growth. Late pinch anticipated the crop cycle, increased gypsophila growth and productivity, benefiting the quality of the stems in length. The greatest thermal sum (TSa) period for early pinch was in the flower elongation and initiation phenological phase (III) and for late pinch in vegetative phase (I). The highest water consumption by plants occurred in phase III, both for accumulated and daily consumption. The total average consumption accumulated during the cycle was 7.05 liters plant-1 and the daily average consumption was 0.309 liters plant-1. In relation to the water use efficiency (WUE) for the production of fresh mass (FM) of stems, the substrates CAC + S10 and CAIN + S10 were superior than pure materials, but did not differ from each other. Regarding the WUE for the dry mass (DM) production of stems and the total of the plants there was no effect of the substrates. Thus, it was verified that the gypsophila plant produced on average 9.36g of DM for each 1 liter of water consumed. In the second experiment, the objective was to evaluate the growth and quality of Lisianthus plants, also, on different substrates based on rice husk (CAC); rice husk in natura(CAIN); CAC (70%) + organic commercial substrate S10 (Beifort®) (30%) and CAIN (70%) + S10 (Beifort®) (30%). The use of CAIN as an isolated substrate material impaired plant growth and prevented flowering by modifying the DM partitioning relationships among plant organs. The CAIN + S10 and CAC substrates can be used for the cut flower lisianthus production in a troughs system, with nutrient solution recirculation, since both provided stems with a high quality standard, especially the first one, which promoted greater plant growth. The results are due to the improvement of the physical and chemical qualities of the CAIN + S10 substrate. The organic compound increased the water retention capacity and CAIN ensured a high porosity to the mixture throughout the crop. Thus, it was verified that gypsophila and lisianthus were adequately adapted to the growing troughs system in with substrates based on rice husk, with leach recirculation.