Around 19% of global power consumption and around 3% of global oil demand is attributable to lighting. After the first incandescent lamp was invented in 1879, more and more energy efficient lighting devices, such as gas discharge lamps, and light-emitting diodes (LED), have been developed during the last century. It is estimated that over 38% of future global lighting energy demand could be avoided by the use of more efficient lamps and ballasts [1]. High intensity discharge (HID) lamps, one category of gas discharge lamp, have been widely used in both commercial and residential lighting applications due to their merits of high efficacy, long life, compact size and good color rendition [2-4]. However, HID lamps require a well-designed ballast to stabilize the negative VI characteristics. A so-called ignitor is also needed to provide high voltage to initiate the gas discharge. Stringent input harmonic current limits, such as the IEC 61000-3-2 Class C standard, are set for lighting applications. It is well-known that high-frequency electronic ballasts can greatly save energy, improve lamp performance, and reduce the ballast size and weight compared with the conventional magnetic ballast. However, a unique phenomenon called acoustic resonance could occur in HID lamps under high-frequency operation. A low-frequency square wave current driving scheme has proved to be the only effective method to avoid acoustic resonance in HID lamps. A typical electronic HID ballast consist of three stages: power factor correction (PFC), DC/DC power regulation and low-frequency DC/AC inverter. The ignitor is usually integrated in the inverter stage. The three-stage structure results in a large size and high cost, which unfortunately offsets the merit of the HID lamp, especially in low-wattage applications. In order to make HID lamps more attractive in low-wattage and indoor applications, it is critical to reduce the size, weight and cost of HID ballasts. This dissertation is aimed at developing a compact HID with an ultra-compact ballast installed inside the lamp fixture. It is a similar concept to the compact fluorescent lamp (CFL), but it is much more challenging than the CFL. Two steps are explored to achieve high power density of the HID ballast. The first step is to improve the system structure and circuit topology. Instead of a three-stage structure, a two-stage structure is proposed, which consists of a single-stage power factor correction (SSPFC) AC/DC front-end and an unregulated DC/AC inverter/ignitor stage. An SSPFC AC/DC converter is proposed as the front-end. A DCM non-isolated flyback PFC semi-stage and a DCM buck-boost DC/DC semi-stage share the semiconductor switch, driver and PWM controller, so that the component count and cost can be reduced. The proposed SSPFC AC/DC front-end converter can achieve a high power factor, low THD, low bulk capacitor voltage, and the desired power regulation with a simple control circuit. Because the number of high-frequency switches is reduced compared to that of state-of-the-art two-stage HID ballast topologies, the switching frequency can be increased without sacrificing high efficiency, so the passive component size can be reduced. The power density of the whole ballast is increased using this two-stage structure. It results in a 2.5 times power density (6 W/in3) improvement compared to the commercial product (2.4 W/in3). The power density of the converter in discrete fashion usually suffers as a result of poor three-dimensional (3D) volume utilization due to a large component count and the different form factor of different components. In the second step, integration and packaging technologies are explored to further increase the power density. A 3D passive integrated HID ballast is proposed in this dissertation. All power passive components are designed in planar shape with a uniform form factor to fully utilize the three-dimensional space. In addition, electromagnetic integration technologies are applied to achieve structural, functional and processing integration to reduce component volume and labor cost. System partitioning, integration and packaging strategies, and implementation of major power passive integration, including an integrated EMI filter, and an integrated ignitor, will be discussed in the dissertation. The proposed integrated ballast is projected to double the power density of the discrete implementation. By installing the HID ballast inside the lamp fixture, the ambient temperature for the ballast will be much higher than the conventional separately installed ballast, and combined with a reduced size, the thermal condition for the integrated ballast will be much more severe. A thermal simulation model of the integrated ballast is built in the IDEAS simulation tool, and appropriate thermal management methods are investigated using the IDEAS simulation model. Experimental verification of various thermal management methods is provided. Based on the thermal management study, a new integrated ballast with improved thermal design is proposed. / Ph. D.

Accepting that one of the main duties of an architect is mastering the tectonic expression of the building and its architectural effect, could there be a mechanism that allows a building or object to change its expression? Could the envelope of a building respond to its exterior context or internal function by changing its appearance? How can the elements of a building screen - whether in wall or roof - change their orientation and configuration to express a response to environments inside or outside? We know that the modern ideas of "wall-free structure" and "transparency" have begun to change the concept of building envelopes. These changes have allowed envelopes to become lighter, less opaque, and more flexible; and as a result the architect has more freedom in exterior expression. In the Seagram Tower, for instance, while I-beams emphasize the vertical effect of the tower, the glass envelope allows the building to change its surface effect from day to night. Having more freedom in the exterior expression of buildings has not only brought transparency but also opened a door for a greater interaction between inside and outside. If, in the past, the exterior walls of a building, limited by structural needs, had to carry its weight, today, analogous with living nature, the use of a structural skeleton gives much more freedom of expression and interaction to the exterior envelope of the buildings. Rooted in the ground and running through the building's mass, instead of outside it, the skeleton takes on the load of the building, and masonry walls can be replaced with partitioning envelopes that are open to freer interpretation. Influenced by engineers and architects like Chuck Hoberman and Santiago Calatrava, my areas of study since 1997 have been focused on the subject of movement in structures and kinetic architectural elements. I have approached this subject from two points of view. While my first study models explored the ways structures can be folded and transformed, this book is about the investigation and study of envelopes and tectonic planes that can be manipulated through operable mechanisms to change their interior or exterior expression. This study is based on the separation between the mechanisms which are the cause of the movements in the models and the kinetic planes in envelopes that have the duty of interaction between inside and outside. If folding of structures in the early stages of my studies resulted in "metamorphosis", my current work is about studying mechanisms that change the surface of an envelope and result in "transmutation". / Master of Architecture

This thesis is the result of insights gained from investigation of the work of Carlo Scarpa and applied to the making of a lamp. Scarpa's work has influenced this work in many ways but most fascinating is the way detail is used. The indirect connection between parts and blending of precious and semiprecious metals conveys its own language. Geometry, one of the governing aspects of his design is a primary characteristic in the composition of spaces. The way different metals are used and the ways different spaces are created are fundamental throughout his work. This project is an attempt to make a lamp which is more than an object. Different metals like steel, brass, copper and aluminum are treated in extreme conditions and the range of different materials are part of a process. In the process of making, the design has taken a few turns. An ongoing dialogue with objects and an empirical process of evaluating and analyzing past objects were used to support the next decision. Often details are designed on the spot in the shop. The Lamp has had two reflectors. The design of the reflector is part of an on going process. Although this object may not be as functionally efficient as other lamps, it has provided an opportunity for discourse and acts as a vehicle for further investigation. Many enjoyable moments are the part of its qualified success and it has open up a wide horizon of architectural gesturing. / Master of Architecture

<p>Gotlands Lampfabrik är en nystartad verksamhet (2009) som drivs av Jonas Kling och Jane Odell centralt på Gotland. De producerar stilrena lampskärmar i ett ljust polymermaterial med papp på båda sidorna.</p><p>Produktionen är liten och görs för hand på plats i den egna fabriken i Väskinde.</p><p>Sortimentet består av 33 lampskärmar och i nuläget producerar inte Gotlands Lampfabrik sina egna lampben eller upphängningar utan köper in dem från fristående parter vilket medför att sortimentet kan uppfattas som spretigt och osammanhängande. Dagens sortiment saknar även vägglampor och ljuskällorna som används är glödlampor som från och med 2009 håller på att urfasas från den Europeiska marknaden efter ett beslut från EU.</p><p><strong> </strong></p><p>Möjligheten att kunna välja lampor från samma serie till sitt hem är både efterfrågat samt kan ge en känsla av sammanhållen och genomtänkt interiör. Genom att göra en produktserie med tillhörande upphängning samt lampben skulle verksamheten kunna profilera sig bättre och få en mer tydlig produktidentitet.</p><p>I ett nära samarbete med Jonas på Gotlands Lampfabrik kom vi gemensamt överens om att skapa en ny produktserie med tydligt släktskap till varandra. I produktserien skulle en taklampa, en golvlampa samt en bordslampa ingå men efter undersökningar tog vi beslutet att byta ut bordslampan till en vägglampa då Gotlands Lampfabrik saknade denna armatur helt. För att tydligare visa på produktseriens tillhörighet till varandra valde jag även att utveckla ett ben till golvlampan. Då vi arbetat bort bordslampan och den möjliga tillhörande lampbenet fann jag det omöjligt att skapa en upphängning för vägglampan som synligt tillhörde resten av produktserien.</p><p>Projektet började med förstudier då jag gjorde en enkätundersökning, marknadsundersökning, trendspaning samt samlade in fakta som sedan legat till grund för arbetet. Efter sammanställning av fakta togs skisser och enklare modeller fram i papper för att presentera mina konceptförslag. Tillsammans med Jonas på Gotlands Lampfabrik diskuterade vi fram ett favoritförslag som resulterade i ytterligare ett koncept som vi båda kände oss nöjda med och sedan producerade jag serien i fabriken. Projektet resulterade i tre lampskärmar, en tak-, en vägg- och en golvlampa med tillhörande lampben för golvlampan. Lampbenet består av ett modulsystem med utbytbara delar där kunden själv får välja vilken form och material som just deras lampben ska ha. Samtliga lampor är försedda med tvinnad textilsladd för en mer exklusiv känsla och är framtagna i det vita plastlaminatet som Gotlands Lampfabrik redan använder. De har även en liten märkning på sladden som visar att den kommer från Gotlands Lampfabrik. Ljuskällan med överlägsna fördelar för projektet blev Philips MasterLED 7W vilket ger en behaglig belysning.</p> / <p>Gotlands Lampfabrik is a new business (2009) run by Jonas Kling and Jane Odell on Gotland in Sweden. They produce stylish lampshades in a bright polymer material with paper on both sides. Their production is small and made by hand in their own workshop in Väskinde with a current collection consisting of 33 lamps.</p><p>Gotlands Lampfabrik currently does not produce their own lamp fixtures or suspensions but instead buys them from other companies, as a result making the collection seem unclear and incoherent.</p><p>The current collection also lacks wall lamps and the light sources are outdated light-bulbs that are slowly being taken off the market after a decision from the EU.</p><p>The possibility to select well designed lamps from a series for your home are both in high-demand and can provide a sense of coherency and well though-out interior design for the consumer.</p><p>By making a new, carefully put together product series and lamp-foot, branding for Gotlands Lampfabrik would be easier and help their lamps have a more clear product identity.</p><p>In a close collaboration with Jonas we both agreed that I would create a new product series for Gotlands Lampfabrik with a distinct design relationship within the new series. The product series would include a hanging lamp, a floor lamp and a table lamp but after some discussion and studies we took the decision to replace the table lamp with a wall lamp since Gotlands Lampfabrik lacked this fixture in their collection.</p><p>The project started with a pilot study where I did market research, a survey and investigated trends within lighting and lamps. After I compiled these facts I made sketches and models and presented them for Gotlands Lampfabrik. Together with Jonas we discussed and picked our favourite concepts which was developed further. The final concept we both agreed on was produced by me in their workshop. The project resulted in three lamps and a modular system for the floor lamp fixture where consumers can select the shape and materials by themselves. All lamps are equipped with a textile cord for a more exclusive feel and are designed in the white plastic laminate that Gotlands Lampfabrik already use in production. The lamps also have a small label on the cord which shows that it comes from Gotlands Lampfabrik. The light source that had most benefits, and was well suited for this project was Philips MasterLED 7W.</p>

Gotlands Lampfabrik är en nystartad verksamhet (2009) som drivs av Jonas Kling och Jane Odell centralt på Gotland. De producerar stilrena lampskärmar i ett ljust polymermaterial med papp på båda sidorna. Produktionen är liten och görs för hand på plats i den egna fabriken i Väskinde. Sortimentet består av 33 lampskärmar och i nuläget producerar inte Gotlands Lampfabrik sina egna lampben eller upphängningar utan köper in dem från fristående parter vilket medför att sortimentet kan uppfattas som spretigt och osammanhängande. Dagens sortiment saknar även vägglampor och ljuskällorna som används är glödlampor som från och med 2009 håller på att urfasas från den Europeiska marknaden efter ett beslut från EU. Möjligheten att kunna välja lampor från samma serie till sitt hem är både efterfrågat samt kan ge en känsla av sammanhållen och genomtänkt interiör. Genom att göra en produktserie med tillhörande upphängning samt lampben skulle verksamheten kunna profilera sig bättre och få en mer tydlig produktidentitet. I ett nära samarbete med Jonas på Gotlands Lampfabrik kom vi gemensamt överens om att skapa en ny produktserie med tydligt släktskap till varandra. I produktserien skulle en taklampa, en golvlampa samt en bordslampa ingå men efter undersökningar tog vi beslutet att byta ut bordslampan till en vägglampa då Gotlands Lampfabrik saknade denna armatur helt. För att tydligare visa på produktseriens tillhörighet till varandra valde jag även att utveckla ett ben till golvlampan. Då vi arbetat bort bordslampan och den möjliga tillhörande lampbenet fann jag det omöjligt att skapa en upphängning för vägglampan som synligt tillhörde resten av produktserien. Projektet började med förstudier då jag gjorde en enkätundersökning, marknadsundersökning, trendspaning samt samlade in fakta som sedan legat till grund för arbetet. Efter sammanställning av fakta togs skisser och enklare modeller fram i papper för att presentera mina konceptförslag. Tillsammans med Jonas på Gotlands Lampfabrik diskuterade vi fram ett favoritförslag som resulterade i ytterligare ett koncept som vi båda kände oss nöjda med och sedan producerade jag serien i fabriken. Projektet resulterade i tre lampskärmar, en tak-, en vägg- och en golvlampa med tillhörande lampben för golvlampan. Lampbenet består av ett modulsystem med utbytbara delar där kunden själv får välja vilken form och material som just deras lampben ska ha. Samtliga lampor är försedda med tvinnad textilsladd för en mer exklusiv känsla och är framtagna i det vita plastlaminatet som Gotlands Lampfabrik redan använder. De har även en liten märkning på sladden som visar att den kommer från Gotlands Lampfabrik. Ljuskällan med överlägsna fördelar för projektet blev Philips MasterLED 7W vilket ger en behaglig belysning. / Gotlands Lampfabrik is a new business (2009) run by Jonas Kling and Jane Odell on Gotland in Sweden. They produce stylish lampshades in a bright polymer material with paper on both sides. Their production is small and made by hand in their own workshop in Väskinde with a current collection consisting of 33 lamps. Gotlands Lampfabrik currently does not produce their own lamp fixtures or suspensions but instead buys them from other companies, as a result making the collection seem unclear and incoherent. The current collection also lacks wall lamps and the light sources are outdated light-bulbs that are slowly being taken off the market after a decision from the EU. The possibility to select well designed lamps from a series for your home are both in high-demand and can provide a sense of coherency and well though-out interior design for the consumer. By making a new, carefully put together product series and lamp-foot, branding for Gotlands Lampfabrik would be easier and help their lamps have a more clear product identity. In a close collaboration with Jonas we both agreed that I would create a new product series for Gotlands Lampfabrik with a distinct design relationship within the new series. The product series would include a hanging lamp, a floor lamp and a table lamp but after some discussion and studies we took the decision to replace the table lamp with a wall lamp since Gotlands Lampfabrik lacked this fixture in their collection. The project started with a pilot study where I did market research, a survey and investigated trends within lighting and lamps. After I compiled these facts I made sketches and models and presented them for Gotlands Lampfabrik. Together with Jonas we discussed and picked our favourite concepts which was developed further. The final concept we both agreed on was produced by me in their workshop. The project resulted in three lamps and a modular system for the floor lamp fixture where consumers can select the shape and materials by themselves. All lamps are equipped with a textile cord for a more exclusive feel and are designed in the white plastic laminate that Gotlands Lampfabrik already use in production. The lamps also have a small label on the cord which shows that it comes from Gotlands Lampfabrik. The light source that had most benefits, and was well suited for this project was Philips MasterLED 7W.

No description available.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This work presents an efficient lighting system using high intensity light emitting diodes (LEDs). The goal is to develop a compact and low cost electronic circuit to drive and control the current of LEDs disposed in a single enclosure. The proposal aims energy saving, high luminous efficacy and high useful life when replacing traditional lighting systems, as dichroic, incandescent or compact fluorescent lamps by LEDs without any change in electrical system installation. Furthermore, an emergency lighting system integrated into the lamp is proposed, allowing the use of the same equipment in the daily activities, supplied by mains, and under a possible break in the electrical network. To supply the LEDs through the mains with universal input voltage and charge the battery, a Buck converter was employed. To supply the LEDs with a continuous current through the battery, a Boost converter was used. The converters operate at high frequency and control the current through the LEDs independently. The battery can be composed of three rechargeable Ni-MH batteries (1,2 V) or a Li-ion battery (3,6 V). The design attends the Brazilian standard and international standard for emergency lighting systems. To validate the proposed idea, the circuits were designed and implemented. The experimental results are shown with the intention to prove the satisfactory behavior of the lamps. / Este trabalho apresenta um sistema de iluminação eficiente empregando diodos emissores de luz (LEDs) de alta intensidade. O objetivo é desenvolver um circuito eletrônico, compacto e de baixo custo, para acionamento e controle da corrente dos LEDs contidos em um único invólucro. A proposta visa a racionalização no uso da energia elétrica através de uma elevada eficácia luminosa e longa vida útil na substituição dos sistemas de iluminação tradicionais, como lâmpadas dicróicas, incandescentes ou fluorescentes compactas, por LEDs sem qualquer alteração na instalação elétrica. Além disso, é proposto um sistema de iluminação de emergência integrado a esta lâmpada, permitindo a utilização de um único equipamento para iluminação convencional, alimentada pela rede elétrica, e sob uma possível falha no fornecimento de energia. Para alimentar os LEDs, através da rede elétrica, com tensão de entrada universal e ainda carregar a bateria, um conversor Buck é empregado. Para alimentar os LEDs em corrente contínua através da bateria, um conversor Boost é utilizado. Os conversores operam em alta freqüência e controlam a corrente nos LEDs de forma independente. A bateria pode ser composta por três pilhas recarregáveis de Ni-MH (1,2 V) ou por uma bateria de Li-Ion (3,6 V). O projeto atende às exigências da norma brasileira (NBR 10898) e da norma internacional (IEEE Std 446-1995) para sistemas de iluminação de emergência. Para analisar a idéia proposta, os circuitos são projetados e implementados. Os resultados experimentais são apresentados de forma a comprovar o comportamento satisfatório das lâmpadas.

Made available in DSpace on 2016-08-17T18:39:33Z (GMT). No. of bitstreams: 1 3168.pdf: 2139042 bytes, checksum: 58468f87414d6567ebe9a007d0fc5966 (MD5) Previous issue date: 2010-07-30 / Financiadora de Estudos e Projetos / Because the close relationship between men and dogs, possibly some ectoparasites of dogs can be observed parasitizing man. The tick Rhipicecephalus sanguineus is an ectoparasite that has the ability to carry and transmit pathogens to humans. This arthropod, that is blood-feeding, is the main biological vector of the bacteria Ehrlichia canis. Currently, the diagnosis of this disease ehrlichiasis is based on blood, biochemical and serological tests, although they are unreliable for diagnosing the disease, since their clinical and clinicopathological features are largely nonspecific. Tetracyclines are commonly used in the treatment of ehrlichiosis, but studies show that some dogs remain positive for E. canis after treatment or after the loss of spontaneous infection. The chronic ehrlichiosis, having high prognosis fails, can result in high mortality. Therefore, more sensitive and reliable tests may help in the selection of dog carrying the disease. The PCR (polymerase chain reaction) has been used successfully in the diagnosis of E. canis. However, this molecular technology requires expensive equipment and specialized personnel to handle, which limits its use in laboratories routines. A more sensitive, specific, and simple to detect the microorganism method is desirable. In this work we develop the technique for detection of the bacterium Ehrlichia canis using the LAMP (Loop- Mediated Isothermal Amplification), which proved being very effective. Specific sequences of the E. canis dsb gene (disulfide bond) were used as target for the tested techniques. Dsb gene was highly specific in order to detect E. canis, since it is divergent of phylogenetically similar bacteria. Performed molecular tests showed a disease incidence greater than that indicated by authors using traditional diagnostic techniques. In the public kennel, 80% of the samples were infected, while in private clinics and veterinary hospital 40% had the disease. The developed diagnostic technique using LAMP is more sensitive than PCR, highly specific and does not require the prior DNA extraction to amplification. In addition, the product of amplification can be seen with the naked eye. We conclude that the diagnosis by the LAMP method enables the specific and high sensitivity identification of infected animals with minimal laboratory settings. These factors make possible the use of this diagnostic methodology for veterinary clinics, laboratories and educational institutions. / Devido à estreita relação entre o homem e o cão, eventualmente alguns ectoparasitas de cães podem ser observados parasitando o homem. O carrapato Rhipicecephalus sanguineus é um ectoparasita que possui a capacidade de carregar e transmitir patógenos aos seres humanos. Esse artrópode, por exercer hematofagia, é o principal vetor biológico e reservatório da bactéria Ehrlichia canis. Atualmente, o diagnóstico da erliquiose é baseado em testes hematológicos, bioquímicos e sorológicos, embora sejam pouco confiáveis para diagnostica-lá, uma vez que suas características clínicas e clinicopatológicas são amplamente inespecíficas. As tetraciclinas são comumente utilizadas no tratamento da Erliquiose, mas estudos mostram que alguns cães permanecem soropositivos para E. canis após o tratamento ou após a perda da infecção espontânea. A Erliquiose crônica por ter falhas de prognóstico, pode resultar em alta mortalidade. Portanto, testes mais sensíveis e confiáveis poderão auxiliar na seleção de cães portadores. A PCR (reação em cadeia da polimerase) tem sido usada com sucesso no diagnóstico da bactéria E. canis. Entretanto, esta tecnologia molecular necessita de equipamentos caros e pessoal especializado para sua execução, o que limita o seu uso na rotina dos laboratórios. Assim uma metodologia mais sensível, específica, e simples de detectar o microorganismo é desejável. Neste trabalho desenvolvemos a detecção da bactéria Ehrlichia canis pena da técnica de LAMP (Loop-Mediated Isothermal Amplification), que se mostrou muito eficaz. Utilizamos sequências específicas do gene dsb (disulfide bond) de E. canis como alvo para as técnicas testadas. O gene dsb mostrou-se altamente específico para a detecção de E. canis, já que é divergente até mesmo das bactérias filogeneticamente próximas. Os testes moleculares realizados mostram uma incidência maior da doença do que aquela preconizada por autores que utilizam técnicas diagnósticas tradicionais. No canil municipal, 80% das amostras estavam infectadas; das clínicas particulares e do hospital veterinário 40% apresentaram a doença pela técnica de PCR. A técnica de diagnóstico por LAMP que foi desenvolvida é mais sensível do que o PCR, altamente específica e não é necessária a extração do DNA para a sua amplificação. Além disso, o produto da amplificação pode ser visualizado a olho nu. Concluímos que o diagnóstico pela metodologia LAMP possibilita a identificação específica e com alta sensibilidade de animais infectados, com mínima estrutura laboratorial. Tais fatores viabilizam a utilização dessa metodologia diagnóstica para clínicas veterinárias, laboratórios e instituições de ensino.

En ökad utbredning av vankomycinresistenta enterokocker (VRE) har setts i Sverige sedan 2007. Bakteriemi orsakad av VRE är mycket svårbehandlad, varför snabbare tillförlitlig resistensdiagnostik är betydelsefullt för att minska dödlighet, vårdtider, vårdkostnader och belastning på sjukvårdssystemet. På mikrobiologilaboratoriet, Region Jönköpings län (RJL), tar idag identifiering av fenotypisk vankomycinresistens vid optimala förhållanden 6 timmar, räknat från att enterokocker konstaterats växa i blodet. Resistensgenerna vanA och vanB, som bland andra orsakar vankomycinresistens hos enterokocker, kan genetiskt verifieras med loop-mediated isothermal amplification men tar idag upp till ett dygn då bakteriekolonier används som analysmaterial i arbetsrutinen på molekylärbiologilaboratoriet, RJL. Syftet med studien var att utvärdera bakteriepellet som analysmaterial för genetisk identifiering av vanA och vanB, på Genie® II Mk2 med eazyplex® VRE basic, hos enterokocker från positiva blododlingar. För att utvärdera bakteriepellet som analysmaterial analyserades isolat av Enterococcus faecium (n=17) och Enterococcus faecalis (n=5) från bakteriepellets tillverkade från simulerade positiva blododlingar med eazyplex® VRE basic på Genie® II Mk2, varpå resultaten jämfördes mot isolatens faktiska närvaro/frånvaro av vanA/vanB. Samstämmigheten av de uppmätta- och de förväntade resultaten var fullständig, vilket indikerar att bakteriepellet med hög tillförlitlighet kan användas som analysmaterial till eazyplex® VRE basic för att påvisa vanA och vanB hos enterokocker i blododlingar. / An increased prevalence of vancomycin-resistant enterococci (VRE) has been observed in Sweden since 2007. Treating bacteremia caused by VRE is difficult, which is why faster, and reliable resistance diagnostics are important. At the Microbiology laboratory, Region Jönköping County, the identification of phenotypic vancomycin resistance under optimal conditions takes 6 hours from when growth of enterococci in blood is determined. The genes vanA and vanB, which among others cause vancomycin resistance, can be genetically verified by loop-mediated isothermal amplification, but takes up to one day since bacterial colonies are used as analysis material. The aim of the study was to evaluate bacterial pellet as an analytical material for genetic identification of vanA and vanB, on Genie® II Mk2 with eazyplex® VRE basic, in enterococci from positive blood cultures. To evaluate the bacterial pellet, isolates of Enterococcus faecium (n=17) and Enterococcus faecalis (n=5) from bacterial pellets made from simulated positive blood cultures were analyzed with eazyplex® VRE basic on the Genie® II Mk2, and the results were compared to the actual presence/absence of vanA/vanB in the isolates. The complete coherence between the expected and measured results indicates that the bacterial pellet can be used as an analytical material for eazyplex® VRE basic.

No description available.