The master thesis deals with theories of temperament from the perspective of their linkages with biological essence of man. In theoretical chapters, it is examined how research of temperament has evolved over time, and there are presented the most important biology- oriented theories, ranging from classical antique temperaments, to Pavlov's differentiation of personalities according the reactivity of nervous system, and finally to Eysenck's, Gray's and Cloninger's modern theories of temperament. Empirical research is dedicated to the testing of interdependencies between temperamental questionnaires. 4Elements Inventory test based on an element approach theory and Cloninger's TCI-R test representing biological approach in temperament observation were analyzed on a research sample, and correlation and regression analysis was performed. If we have to assign the most similar dimensions of both tests to each other according to content analysis, we would expect relationships between the Harm Avoidance and Fire (negative), Novelty Seeking and Air, Reward Dependency and Water and Persistence and Earth. The relationship of Novelty seeking and Air (r = 0.73) and negative relationship of Harm Avoidance and Fire (r = -0.74) were confirmed as the strongest relationships. The correlation between Reward...