Master of Science / Department of Agronomy / David B. Mengel / Nitrogen fertilizers play an essential role in agricultural production in Kansas, particularly in row crops such as corn (Zea mays L.) and grain sorghum (Sorghum bicolor (L.) Moench). A good portion of the corn and grain sorghum grown in Kansas is typically grown using no-till production systems. These systems leave a large amount of surface residue on the soil surface, which can lead to ammonia volatilization losses from surface applied urea-containing fertilizers and immobilization of N fertilizers placed in contact with the residue. Leaching and denitrification can also be a problem on some soils. Current nitrogen prices, as well as concerns over environmental stewardship, are forcing producers to make smarter choices in the fertilizer products used as well as when and how the materials are applied, to optimize their nitrogen use efficiency. A common practice throughout Kansas is to apply N fertilizers prior to planting, sometimes up to 6 month prior to planting. What affect does this practice have on nitrogen availability to the growing crop? Current Kansas State University (KSU) soil test fertilizer recommendations assume 50% nitrogen use efficiency. This means of every pound of nitrogen applied only half will be utilized by the plant and turned into valuable grain. Possible solutions to help increase nitrogen use efficiency are the use of nitrogen additives which are currently on the market and claim to reduce nitrogen loss through denitrification and volatilization as well as the use of timing and application of fertilizers to further increase nitrogen use efficiency. The objective of this study is to evaluate different N fertilizer products, as well as additives and application practices and determine whether specific combinations can improve yield and N use efficiency of no-till corn and grain sorghum. The long-term goal of this study is to quantify some of these relationships to assist farmers in selecting specific combinations that could enhance yield and profitability. In this study five tools for preventing N loss were examined: fertilizer placement, or placing N below the soil surface or in bands on the residue-covered soil surface to reduce immobilization and/or volatilization; use of a urease inhibitor Agrotain (NBPT) that blocks the urease hydrolysis reaction that converts urea to ammonia and potentially could reduce ammonia volatilization; the use of a commercially available additive, Agrotain Plus, that contains both a nitrification inhibitor (DCD) and a urease inhibitor to slow both urea hydrolysis and the rate of ammonium conversion to nitrate and subsequent denitrification or leaching loss; use of a commercial product NutriSphere-N, which claims urease and nitrification inhibition; and the use of a polyurethane plastic-coated urea to delay release of urea fertilizer until the crop can use it. The ultimate goal of using these practices or products is to increase N uptake by the plant and enhance yield. An important measurement that was developed for this research was the use of a greenleaf firing index which used the number of green leaves below the ear at pollination as a key measurement in determining the effectiveness of fertilizer placement, application method, application timing and the use of nitrogen additives. If significant differences in lower leaf nitrogen stress are found, the potential exists to further develop this index and correlate differences observed with key parameters of nitrogen uptake such as ear-leaf nitrogen concentration, total nitrogen uptake and grain yield. Results observed from this research show that the potential to increase nitrogen use efficiency and reduce nitrogen loss do exist with the use of certain nitrogen additives, application methods and application timing. When conditions are conducive for nitrogen loss the use of currently available tools to protect nitrogen from volatilization, immobilization and/or denitrification loss significantly increased yields in the corn experiments. Results from the grain sorghum research indicate that when N losses limit yield, the use of products and practices enhance yield. In locations where nitrogen loss is minimal or low yields limit nitrogen response, the use of these practices was not found to be helpful.

Doctor of Philosophy / Department of Biochemistry / Lawrence C. Davis / The fate and bioavailability of organic contaminants in plants is a major ecological and human health concern. Current wet chemistry techniques that employ strong chemical treatments and extractions with volatile solvents, such as GC-MS, HPLC, and radiolabeling, although helpful, degrade plant tissue resulting in the loss of spatial distribution and the production of artifacts. Synchrotron radiation infrared microspectroscopy (SR-IMS) permits direct analysis of plant cell wall architecture at the cellular level in situ, combining spatially localized information and chemical information from the IR absorbances to produce a chemical map that can be linked to a particular morphology or functional group. This study demonstrated the use of SR-IMS to probe biopolymers such as cellulose, lignin, and proteins in the root tissue of hydroponically grown sunflower and maize plants as well as to determine the fate and effect of several organic contaminants in those root tissues. Principal components analysis (PCA), a data compression technique, was employed to reveal the major spectral variances between untreated and organic contaminant treated root tissues. Treatment with 1H-benzotriazole (BT) caused alterations to the lignin component in the root tissue of plants. The BT was found in xylem and epidermal tissue of sunflower plants but not associated with any particular tissue in maize roots. 2,4-dinitrotoluene (2,4-DNT) and 2,6-dinitrotoluene (2,6-DNT) altered the pectin and polysaccharide structure in both maize and sunflower. SR-IMS revealed the reduction of DNTs to their aromatic amine form in the vascular and epidermal tissues at low concentration. At high concentration, DNTs appeared to be associated with all the plant tissues in maize and sunflower. Exposure of sunflower and maize to 2,6-dichlorophenol (2,6-DCP) caused alterations to the polysaccharide and protein component of the root tissue. In some cases, phenolic compounds were observed in the epidermal tissue of maize and sunflower roots. The results of this research indicate that SR-IMS has the potential to become an important analytical tool for determining the fate and effect of organic contaminants in plants.

Doctor of Philosophy / Department of Plant Pathology / Bikram S. Gill / The wild relatives of crop plants are sources of useful genes, but such genes when transferred to agricultural crops are often associated with deleterious traits. Because most of the recombination and the disease resistance genes are localized towards the ends of wheat chromosomes, cryptic terminal alien segments, carrying rust resistance genes, were transferred from Aegilops geniculata (UgMg) and Ae. triuncialis (UtCt) into common wheat without the usual linkage drag. The alien segment with the leaf rust and stripe rust resistance genes Lr57 and Yr40 in translocation T5DL•5DS-5MgS(0.95) was found to be less than 3.3 cM in genetic length and spans less than four overlapping BAC/PAC clones of the syntenic rice chromosome arm 12L. The alien segment with leaf rust resistance gene Lr58, transferred from Ae. triuncialis, was found to be less than 5% of the chromosome arm 2BL of wheat in T2BS•2BL-2tL(0.95), further suggesting that it is feasible to transfer small alien segments with disease resistance genes. Resistance genes Lr57, Yr40 and Lr58 were transferred to Kansas hard red winter wheat cultivars by backcrossing and marker assisted selection. Tillering, a key component of grain yield, and seed color which influences seed dormancy and pre-harvest sprouting in wheat, are agronomically important domestication traits in wheat. A tiller inhibition mutant with monoculm phenotype was isolated and the mutated gene (tin3) was mapped on the distal region of chromosome arm 3AmL of T. monococcum. As a first step towards isolating candidate gene(s), the tin3 and the seed color gene (R-A1) of chromosome 3A were mapped in relation to physically mapped ESTs and STS markers developed based on synteny with rice. Physically mapped wheat ESTs provided a useful framework to identify closely related rice sequences and to establish the most likely syntenous region in rice for the wheat tin3 and R-A1 region. Comparative genomic analysis of the tin3 and R-A1 genomic regions with the corresponding region in rice localized the tin3 gene to a 324 kb region spanned by two overlapping BACs and the R-A1 gene was mapped to a single BAC of the colinear rice chromosome arm 1L.

Master of Science / Department of Agronomy / Scott A. Staggenborg / Irrigated cropping systems on the High Plains are dominated by water intensive continuous corn (Zea mays L.) production, which along with other factors has caused a decline in the Ogallala aquifer. Potentially demand for water from the aquifer could be decreased by including drought tolerant crops, like grain sorghum (Sorghum bicolor L.) and cotton (Gossypium hirsutum L.), in the cropping systems. This study calibrated the CERES-Maize, CERES-Sorghum, and CROPGRO-Cotton models for the High Plains and studied the simulated effects of different irrigation amounts and initial soil water contents on corn, cotton, and grain sorghum. Input files for calibration were created from irrigated and dryland research plots across Kansas. Information was collected on: soil physical properties, dry matter, leaf area, initial and final soil water content, management, and weather. CERES-Maize simulated grain yield, kernel number, ear number, and seed weight across the locations with root mean square errors (RMSE) of 2891 kg ha-1, 1283 kernels m-2, 1.6 ears m-2, and 38.02 mg kernel-1, respectively. CERES-Sorghum simulated grain yield, kernel number, head number, and seed weight with RMSEs of 2150 kg ha-1, 5755 kernels m-2, 0.13 heads m-2, and 4.51 mg kernel-1. CROPGRO-Cotton simulated lint yield and boll number with RMSEs of 487 kg ha-1 and 25.97 bolls m-2. Simulations were also conducted with CERES-Maize, CERES-Sorghum, and CROPGRO-Cotton to evaluate the effects of irrigation amounts and initial soil water content on yield, evapotranspiration (ET), water use efficiency (WUE), available soil water at maturity, and gross income per hectare. Simulations used weather data from Garden City, KS from 1961 to 1999. Irrigation amounts were different for all variables for corn and grain sorghum. For cotton, yield, WUE, soil water, and gross income were not different between the top two irrigation amounts. For corn and grain sorghum, initial soil water content was only different at 50% plant available water. Initial soil water had no affect on cotton, except for ET at 50%. Simulations showed that cotton yields are similar at lower irrigation. Also, cropping systems that include cotton have the potential to reduce overall irrigation demand on the Ogallala aquifer, potentially prolonging the life of the aquifer.

Master of Science / Department of Agronomy / Gary M. Pierzynski / Soil chemical and physical properties beneath cattle feedlot pens are largely unstudied. This project was conducted to survey select soil chemical and physical properties of soil beneath active open air cattle feedlots. At four cattle feedlots in Kansas, the concentrations of NH4-N, organic-N, organic-C, Cl-, and P were high at the surface and rapidly decreased within 1.00 m. At three of the four feedlots, NO3-N was generally below background concentration (4.1 mg kg-1) while one feedlot had a >75 mg kg-1 increase in the top 1.00 m. Based on feeding data, only a small percent (7.9 to 1.2) of the total N deposited on the surface was found in the top 1.00 m below the pen surface for a range of 25 to 60 years of operation. While in use, these feedlots do not appear to have a high potential for groundwater pollution from NO3-N leaching. However, if they were to become inactive they may pose a severe threat to groundwater quality from organic-N mineralization and NH4-N nitrification. If feedlots were closed and the land could be largely remediated by removing a layer of soil, these feedlots would have an average 48% profile N removed in a 0.25 m thick layer. A chamber, a modified vacuum desiccator, was tested for the investigation of NH3 volatilization from soil in the laboratory. Ammonia volatilization at the soil surface is dependent on air flow, soil and air temperatures, soil water content, pH, the concentrations of NH3 and NH4+ in the air and soil solution, and factors affecting soil temperature including humidity. This chamber was built to control and/or quantify as many of these variables as possible. A technique for quantifying and predicting NH3 volatilization is important because AFOs are one of the largest contributors of NH3 to the atmosphere, which can cause acid precipitation and particulate matter deposition downwind from the operation. The chambers created allowed for repeated measurements with little error and appear to be a feasible, inexpensive apparatus to investigate NH3 volatilization mechanisms. Using synthetic urine as an N source, NH3 volatilization was affected by initial soil moisture content and soil texture and may be affected by initial soil pH. This chamber has promise to provide excellent data to assist the efforts being made to understand and model NH3 volatilization from feedlot pens.

Doctor of Philosophy / Department of Agronomy / Clenton E. Owensby / Most rangelands are harvested at some point during the year and removal of plant leaf area and biomass alters a host of ecosystem processes including gas exchange. An experiment was conducted in 2005 and 2006 to study the effects of clipping tallgrass prairie at different dates on water vapor and CO2 fluxes. A portable, non-steady-state chamber was designed to measure CO2 and water vapor fluxes from small plots in less than 40 s. A combination of sunlit and shaded readings allowed measurements of net carbon exchange (NCE) and ecosystem respiration (RE); by summing NCE and RE, gross canopy photosynthesis (GCP) was calculated. Throughout the two-year study, the chamber had a minimal effect on microclimate, i.e., average chamber temperature increased 2.9° C, while chamber pressure increased only 0.3 Pa during measurements, and photosynthetically active radiation attenuation was 10%. The immediate effect of all clipping treatments was a loss of leaf area that led to reductions in GCP, NCE, and RE and in most cases decreased water vapor flux. Further patterns of carbon flux were governed by the amount of water stress during canopy development, while water vapor flux rates varied with water availability. Canopies that developed during periods of low water stress quickly increased carbon flux rates following precipitation after a mid-season drought. However, flux rates of canopies, which developed during the mid-season drought, responded considerably slower to subsequent water availability. A separate experiment was conducted from June-October of 2006 to estimate GCP, leaf area index (LAI), and total aboveground biomass with a hyperspectral radiometer. Indices such as the Normalized Difference Vegetation Index and the Simple Ratio were used to estimate LAI and biomass had poor correlations with measured values. However, GCP was significantly correlated to all six indices derived in this study. While GCP measured from June-October was significantly correlated with all indices, removal of the senesced canopy scans recorded during October greatly increased the relationship.

Master of Science / Department of Biological & Agricultural Engineering / Robert E. Wolf / Traditionally, growers spray uniform application of pesticides over the target area regardless of variations in pest infestations. In recent years, variable rate application (VRA) technologies have made it possible to apply pesticides in variable rates across the field. In pesticide application, nozzles play a vital role. In general, pesticides are applied using conventional nozzles. Most conventional nozzles vary flow rates only over a 2:1 range when operated within the recommended pressure range due to a fixed spray orifice. Conventional nozzles vary droplet sizes tremendously when there are speed and application rate changes which results in inefficient application. Conventional nozzles have limitations when used for VRA. A new nozzle called Varitarget nozzle (U.S. Patent No. 5,134,961) was developed and marketed by Bui, (2005) to overcome the limitations with conventional nozzles. Varitarget nozzles have a variable orifice that changes in size in response to pressure changes, allowing varying flow rates with a minimal change in droplet size. Laboratory tests and field tests were conducted to study the performance of Varitarget nozzle. Varitarget black/blue and clear/yellow caps were evaluated in this study. Lab studies were conducted to measure Varitarget characteristics compared to conventional nozzles. The flow rate ratios of Varitarget nozzle black and clear caps were 12:1 and 10:1 while the conventional nozzles produced flow rate ratios ranging from only 3:1 to 4:1. The measured flow rate of Varitarget nozzle black and clear caps was similar to that published by the manufacturer up to 40 psi and varied higher after 40 psi. Both Varitarget black and clear cap nozzle was within the standard VMD requirements until 40 psi and showed increasing trend while the conventional nozzles matched the standard VMD requirements. The VT black and clear cap nozzles showed better coverage at higher pressures when compared to conventional nozzles. CV values for VT black and clear capped nozzles were less than 10% which indicates capability of good uniform distribution. Spray angle of 110 degrees for VT black and clear capped nozzles was consistent over a range of pressures. Field studies were also conducted to compare the Varitarget to conventional nozzles. In the varying speed study, droplet size varied from 498 to 621 microns with a SD of 47.50 for VT black nozzle and 465 to 599 microns with a SD of 54.08 for VT clear cap nozzle as the speed varied from 4 to 12 mph. In the varying application rate study, The droplet size varied from 432 to 510 microns with a SD of 27.84 for VT black nozzle and 355 to 452 microns with a SD of 39.80 as the application rate varied from 4 to 12 GPA. In both studies, the observed pressure range required for spraying was minimum and varied slightly.

Master of Science / Department of Entomology / David C. Margolies / James R. Nechols / The study of how extrinsic factors affect the foraging efficiency and behavior of predaceous arthropods like Phytoseiulus persimilis is important to understand their various processes of acquiring prey, mates, refuges, oviposition sites, and overcoming obstacles posed by the environment. Many intrinsic and extrinsic factors affect predator foraging efficiency and behavior. One of the most influential extrinsic factors may be the host plant on which herbivorous prey are found. Recent studies suggest that plant architecture plays an important role in tritrophic interactions. In this work, I studied the effects of cucumber plant architecture and prey distribution on the foraging efficiency (prey-finding time and prey-consumption rate) and behavior (time allocated between moving, resting and feeding) of P. persimilis. Plant architecture represented differences in leaf number and size; however, all plants had the same total surface area. Plants with 6 small leaves (ca. 82.98 square cm each) were considered as complex architecture, whereas plants with only 2 large leaves (ca. 240.60 square cm each) were considered as simple. The prey distributions were: prey patch on a single basal leaf (closest leaf to the soil) and prey patch on all leaves. The foraging efficiency was assessed by measuring prey-finding time and prey-consumption rate, whereas the behavior was assessed by conducting observational studies on specific foraging activities. When placed either on the top or at the base of the plant P. persimilis encountered prey more rapidly (interval 0-30 minute) on complex and simple plants with prey patches distributed on all leaves. Differences in prey density (number of prey per leaf) had no effect on the prey-finding time of P. persimilis. The predator consumed more eggs on complex plants with prey patches distributed on all leaves. Phytoseiulus perismilis tended to find prey patches more quickly as well as consume more eggs on leaves close to its release point. Furthermore, the predator was observed to lay more eggs on leaves where it had consumed higher number of prey eggs. The dimensions of individual parts of the plant e.g., stem, petiole and leaf, affected the time allocated by P. persimilis between searching, resting and feeding. The predatory mite spent more time foraging on the stems and petioles of the simple plants whereas on complex plants it spent more time foraging on the leaves.

Master of Science / Department of Entomology / Larry L. Buschman, Lawrent Buschman / This study evaluated whether using a cover crop with corn would increase the threat from spider mites in western Kansas because cover crops may serve as a winter host. This study also evaluated whether a cover crop could affect corn rootworm and other ground dwelling arthropods in the cornfield. In the first study, downy brome, Bromus tectorum L., was used as the winter cover crop. There were two trials repeated for three years each. Each trial included: two amounts of irrigation, downy brome, and herbicide to control weeds. In the first trial there were no significant differences in corn rootworm, Diabrotica virgifera LeConte, damage across treatments, because there were no differences in brome residue across the treatments. In the second trial, corn rootworm damage was significantly more in plots with higher amounts of downy brome residue. There were no differences in numbers of spider mites: Banks grass mites, Oligonychus pratensis (Banks) or twospotted spider mites, Tetranychus urticae Koch, across treatments. Spider mite populations appeared to be suppressed by the predatory mite Neoseiulus spp., which also overwintered in the cover crop. Corn rootworm samples taken from a no-till irrigation experiment were variable among irrigation treatments but indicated a trend for rootworm damage to increase with increasing irrigation. In the second study, winter wheat, Triticum aestivum L., was used as the winter cover crop. There were three trials repeated for three years each. Each trial included two amounts of irrigation and winter wheat and three amounts of herbicide to control weeds. Upon completion of the agronomy trials, the plots were split into two subplots and one was tilled. Pitfall traps were installed to capture ground dwelling arthropods: (Coleoptera: Carabidae), wolf spiders (Araneae: Lycosidae) and crickets (Orthoptera: Gryllidae). Four carabid genera were more common under no-till conditions. One was more common in tilled plots. Five carabid genera were more common in plots with a history of high weed densities. Two carabid genera were more numerous in plots with the history of a cover crop. Crickets were more common under no-till conditions. Wolf spiders were more common in no tillage with a history of a cover crop.

Master of Science / Department of Agronomy / Scott A. Staggenborg / Spatial variability in plant available water can be caused by uncontrollable factors such as topography and soil texture as well as controllable factors such as residue management. Research located on the High Plains evaluated the impact of wheat (Triticum aestivum L.) stubble height on snow catch, plant available water at seeding, and optimal corn seeding rates. Treatments consisted of stripper harvest height of 71 cm (28 in.), cut heights of 25 cm (10 in.), and 10 cm (4 in.) Measured snow depths were significantly different among treatments (p<0.0001) with equivalent precipitation of 5.77 (2.27), 3.25 (1.28), and 1.73 cm (0.68 in.) for the stripped, 25 cm, and 10 cm heights respectively. Available soil water at planting increased 24% as stubble height increased from 10 to 71 cm (4 to 28 in) in one year of the study. Two corn hybrids of varying maturity (97 and 108 days) were planted into the stubble treatments at seeding rates ranging from 2.47 to 5.43 plants m[superscript]-2 (10 to 22 000 plants ac[superscript]-1). In the dry year, the long season hybrid responded positively to increasing population in tall stubble and negatively in short stubble. Yield of the short season hybrid increased with increasing stubble height and was mostly unresponsive to population. Grain yields of both hybrids responded positively to increasing plant population in a wet year. Treatments also affected the yield components of yield plant[superscript]-1, kernel weight, and kernels plant[superscript]-1. Managing seeding rates for uncontrollable factors was attempted with small-plot and field scale research across 3 fields in northeast Kansas. A relationship between soil electro-conductivity (EC) and measured water holding capacity values was developed for one study field. This quadratic relationship was significant (p<0.0001) and explained variability in water holding capacity with respect to EC quite well (R[superscript]2=0.6239). Responses from small plots showed that sites differing in population response characteristics could be identified. Field scale data was used to derive a function describing optimal seeding rate with respect to soil EC. In the field under study, optimal seeding rates varied from 3.08 to 8.74 plants m[superscript]-2 (12 500 to 35 375 plants ac-1).