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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Cryptosporidium spp. (Tyzzer, 1907) em peixes-boi marinhos (Trichechus manatus) (Linnaeus, 1758) e peixes-boi amazônicos (Trichechus inunguis) (Natterer, 1883) no Brasil

BORGES, João Carlos Gomes 12 February 2007 (has links)
Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:47:32Z No. of bitstreams: 1 Joao Carlos Gomes Borges.pdf: 2198512 bytes, checksum: de1f2ccf01e9496dcc35acd9a6874feb (MD5) / Made available in DSpace on 2016-10-13T12:47:32Z (GMT). No. of bitstreams: 1 Joao Carlos Gomes Borges.pdf: 2198512 bytes, checksum: de1f2ccf01e9496dcc35acd9a6874feb (MD5) Previous issue date: 2007-02-12 / The aim of the present study was to verify the occurrence of oocysts of Cryptosporidium spp. in Antillean manatees (Trichechus manatus) and Amazonian manatees (Trichechus inunguis) from Brazil, and also evaluate the hydric transmission of the coccidium and the influence between this pathogen and the staff involved directly in captive animal care and management. Fecal samples were collected from 34 Antillean manatees, 12 Amazonian manatees and also 21 employees involved in the management activities. Water samples, used to the manatees consumption and management were also collected. The fecal samples were processed by sedimentation in formol-ether and Kinyoun technique. Before the water samples were processed, this was submitted to a filtration process in membrane (diameter of 47 mm, porosity of 3 μm) under negative pressure. At the end of the analyzing operations, the positive samples were submitted to the Direct Immunoflorescence Test. The results obtained by light and fluorescence microscopy analysis showed alcohol-acid-resistant structures compatible with oocyst of Cryptosporidium spp. in 25% (34/136) and 4,30% (05/115) of thematerial proceeding from Antillean manatees and Amazonian manatees, respectively; 23,80% (10/42) of the material obtained from the employees; and 66,67% (04/06) of the water samples analyzed. The results showed the possibility of Antillean manatees and Amazonian manatees could become infected with Cryptosporidium spp., and participate in the epidemiology of this waterborne disease by eliminate of viable oocyst in water environment wich can infect man and a wide range of domestic and wild animals. The hand of the frequency of Cryptosporidium spp. in staff involved directly in captive animal care and management could be the way to prevent the infections the manatee’s species. / O objetivo neste trabalho foi verificar a ocorrência de oocistos de Cryptosporidium spp. em peixes-boi marinhos (Trichechus manatus) e peixes-boi amazônicos (Trichechus inunguis) no Brasil, além de avaliar a transmissão hídrica deste coccídio e a sua influência com os funcionários diretamente envolvidos no manejo dos animais mantidos em cativeiro. Para tanto foram coletadas amostras fecais provenientes de 34 peixes-boi marinhos, 12 peixes-boi amazônicos e 21 funcionários envolvidos em atividades de manejo, bem como amostras de água superficial destinada a manutenção e consumo dos animais. As amostras fecais foram processadas através da sedimentação pelo formoléter e coradas pela técnica de Kinyoun. No que concerne as amostras de água, estas foram submetidas ao processo de filtração em membranas (47 mm de diâmetro e 3 μm de porosidade) sob pressão negativa. No final das análises, as amostras positivas foram submetidas ao Teste de Imunofluorescência Direta. Os resultados obtidos namicroscopia de luz e fluorescência revelaram oocistos de Cryptosporidium spp. em 25% (34/136) e 4,30% (05/115), sendo inerentes respectivamente aos peixes-boi marinhos e amazônicos, 23,80% (10/42) do material proveniente dos funcionários e 66,67% (04/06) das análises de água. Os resultados finais apontam a possibilidade dos peixes-boi marinhos e peixes-boi amazônicos infectarem-se com Cryptosporidium spp., e participar da epidemiologia das doenças veiculadas pela água, através da eliminação de oocistos viáveis ao ambiente que podem infectar o homem e um grande número de animais domésticos e silvestres. O controle da freqüência de infecções de Cryptosporidium spp. nos funcionários diretamente envolvidos no manejo e cuidados dos animais mantidos em cativeiro pode ser um dos caminhos para prevenir a infecção nas espécies de peixes-boi.

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