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Optical studies of a slow-position beam李銘, Li, Ming. January 1995 (has links)
published_or_final_version / Physics / Master / Master of Philosophy
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Optical studies of a slow-position beam /Li, Ming. January 1995 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1996. / Includes bibliographical references (leaf [86-87]).
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Femtosecond cellular transfection using novel laser beam geometriesTsampoula, Xanthi January 2009 (has links)
In this thesis, femtosecond (fs) cellular transfection of Chinese Hamster Ovary (CHO) cells was performed using a tightly focused Gaussian beam. The beam focus was positioned on the cell membrane and three laser doses, each of 40 ms duration, were delivered allowing for the formation of a highly localized pore on the cell membrane. The membrane pore, induced as a result of a multiphoton process known as photoporation, permitted the surrounding DNA to diffuse into the cell cytoplasm. 48 hours after laser irradiation, the viable photoporated cells expressed a red fluorescent protein. The topography of a photoporated cell, targeted with tightly focused fs pulses, was also monitored as a function of the input power using Atomic Force Microscopy. Following this, I generated and implemented a “non-diffracting” quasi-Bessel beam (BB) by means of a conical shaped lens, the axicon, which successfully provided an alternative route for photoporation to the highly divergent Gaussian beam. A comparison was given between the two beam approaches for photoporation. The “non-diffracting” character of the BB resulted in the first successful attempt towards automating optical transfection. This was achieved by using an axicon and a spatial light modulator (SLM) to provide phase modulation on the annular spatial spectrum field of the BB. This approach provided control over the lateral and axial position of the beam with respect to the cell membrane, allowing for point and click photoporation. Successful photoporation of CHO cells was also demonstrated using for the first time an axicon tipped optical fibre. The applicability prospects of this method are significant, ranging from potential endoscopic embodiments of the technique to advanced studies of tissue properties in vitro and in vivo.
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Analysis of two problems related to a focused beam measurement systemPetersson, L. E. Rickard 12 1900 (has links)
No description available.
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The adaptive control of optical beam jitter /Watkins, R. Joseph. January 2004 (has links) (PDF)
Thesis (Ph. D. in Mechanical Engineering)--Naval Postgraduate School, December 2004. / Thesis advisor(s): Brij N. Agrawal, Young S. Shin. Includes bibliographical references (p. 163-165). Also available online.
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Multiple beam correlation using single-mode fiber optics with application to interferometric imaging.Shaklan, Stuart Bruce. January 1989 (has links)
A study of the application of single-mode fiber optics to the multiple-beam interferometric recombination problem is presented. In the laboratory, the fibers have been used in wide bandwidth, two-arm, Mach-Zehnder test interferometers as well as a 5-telescope imaging interferometer connected to an all-fiber beam combiner. Based upon these experiments and some theoretical studies it is shown that fiber optics and fiber optic components such as directional couplers provide an excellent alternative to conventional optics such as mirrors, beamsplitters, and relay lenses. The equations describing the measurement of the complex degree of coherence in an interferometer with a single-mode fiber in each arm are derived. The equations reveal an important feature of the fibers: they filter phase fluctuations due to aberrations and turbulence at the input and convert them to intensity fluctuations at the output. This leads to a simplification of the calibration of measured visibilities. The coupling efficiency of light which has passed through a turbulent atmosphere is also studied as a function of fiber parameters and turbulence conditions for both image motion stabilized and non-stabilized cases. For the former case, coupling efficiency remains greater than 50% as long as telescope diameter is no larger than the turbulence coherence length. Beam combination architectures using arrays of directional couplers are fully discussed. Arrays accommodating up to 20 input beams are presented. The arrays require only N detector pixels for N input beams. A scheme of temporal multiplexing of the phase of each beam is used to identify individual fringe pairs. One possible scheme allows wide bandwidths even for large numbers of beams. A 5-telescope interferometer has been constructed and connected to an all-fiber beam combiner. Two extended objects were observed and reconstructed using standard radio astronomy VLBI software. The interferometer and beam combiner had good thermal and polarization stability and high throughput. Reconstructed images had dynamic ranges of about 50.
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Simultaneous optimization of beam positions for treatment planning and for image reconstruction in radiotherapyWidita, Rena, Physics, Faculty of Science, UNSW January 2006 (has links)
From one treatment to the next, considerable effort is made to accurately position radiotherapy patients according to their treatment plans. However, some variation is unavoidable. The target volume and the organs at risk may also move within the patient and/or change shape during the treatment. Thus, it is important to be able to verify the success of the treatment by determining the position of patient and the dose deposited in the patient at each fraction. One possibility to achieve this, particularly when equipment, time and budgets are limited, would be to collect limited information while the patient is on the treatment couch. This research was aimed to develop a method for optimum beam position determination, for each patient-specific case. The optimized beam positions would balance the both treatment planning and image reconstruction, so that the patient???s image can be obtained during the treatment delivery using the information collected from the same angles as used for treatment. This will allow verification of the dose deposited in the patient for every fraction. Using a limited number of angles for image reconstruction, the dose to the patient can be minimized. This work has two major parts, beam position optimization for image reconstruction and beam position optimization for treatment planning. These two optimizations are then combined to obtain the optimum beam position for both image reconstruction and treatment planning. An objective function, projection correlation, was developed to investigate the image reconstruction method using limited information. Another objective function, the average optimization quality factor, was also introduced to optimize beam positions for treatment planning. Two optimization methods, the gradient descent method and the simulated annealing based on these objective functions were used to determine the beam angles. The results show that the projection correlation presents several advantages. It can be applied without any iterations, and it produces a fast algorithm. The present research will allow selection of the optimum beam positions without excessive computational cost for treatment planning and imaging. By combining the projection correlation and the average optimization quality factor together with more advanced image reconstruction software this could potentially be used in a clinical environment.
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Simultaneous optimization of beam positions for treatment planning and for image reconstruction in radiotherapyWidita, Rena, Physics, Faculty of Science, UNSW January 2006 (has links)
From one treatment to the next, considerable effort is made to accurately position radiotherapy patients according to their treatment plans. However, some variation is unavoidable. The target volume and the organs at risk may also move within the patient and/or change shape during the treatment. Thus, it is important to be able to verify the success of the treatment by determining the position of patient and the dose deposited in the patient at each fraction. One possibility to achieve this, particularly when equipment, time and budgets are limited, would be to collect limited information while the patient is on the treatment couch. This research was aimed to develop a method for optimum beam position determination, for each patient-specific case. The optimized beam positions would balance the both treatment planning and image reconstruction, so that the patient???s image can be obtained during the treatment delivery using the information collected from the same angles as used for treatment. This will allow verification of the dose deposited in the patient for every fraction. Using a limited number of angles for image reconstruction, the dose to the patient can be minimized. This work has two major parts, beam position optimization for image reconstruction and beam position optimization for treatment planning. These two optimizations are then combined to obtain the optimum beam position for both image reconstruction and treatment planning. An objective function, projection correlation, was developed to investigate the image reconstruction method using limited information. Another objective function, the average optimization quality factor, was also introduced to optimize beam positions for treatment planning. Two optimization methods, the gradient descent method and the simulated annealing based on these objective functions were used to determine the beam angles. The results show that the projection correlation presents several advantages. It can be applied without any iterations, and it produces a fast algorithm. The present research will allow selection of the optimum beam positions without excessive computational cost for treatment planning and imaging. By combining the projection correlation and the average optimization quality factor together with more advanced image reconstruction software this could potentially be used in a clinical environment.
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Beam scanning offset Casegrain reflector antennas by subreflector movement /LaPean, James William. January 1993 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1993. / Vita. Abstract. Includes bibliographical references. Also available via the Internet.
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A high gain tri-reflector antenna configuration for beam scanning /Werntz, Paul C., January 1993 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1993. / Vita. Abstract. Includes bibliographical references. Also available via the Internet.
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