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RNA in all forms of life contain a myriad of post-transcriptional modifications. These modifications are important for processing and structural reasons, and includes 2’-O-methylation and pseudouridylation. Some of these modifications are the product of stand-alone proteins and others are the product of RNA-protein complexes.In eukaryotic and archaeal cells, the Box C/D ribonucleoprotein complex is one of the complexes responsible for 2’-O-methylation activity. In Archaea, this complex consists of the enzymatic protein, Fibrillarin, plus two other structural proteins, Nop5 and L7Ae, along with a guide RNA, which all come together to modify a specific target RNA sequence. A methyl group is added to the 2’ hydroxyl of the nucleotide’s ribose sugar. These modifications are found both in ribosomal RNA and tRNAs. This work focuses on the interactions between Nop5, Fibrillarin, and the guide RNA used in this complex. The objective was to identify important amino acid sequences in these proteins which are essential for the operation of the complex. The size of the archaeal Box C/D complex is also investigated using size chromatography.Similarly, Pus10 is one protein responsible for pseudouridylation in Archaea and eukaryotes. Pseudouridylation is the isomerization of uridine (U) to pseudouridine (Ψ). Pus10 is known to produce Ψ54 and Ψ55 in the TΨC loop of some archaeal and eukaryotic tRNAs. However, current research suggests it could have additional jobs in the cellular lifecycle, such as roles in apoptosis and the regulation of eukaryotic cell cycle. During mammalian cell apoptosis, Pus10 translocates from the nucleus to the cytoplasm and is believed to be involved in cytochrome c release. This is suspected to be related to Caspase-3’s role in apoptosis. Caspase-3 is activated in both the extrinsic and intrinsic apoptotic pathways. It has been shown that activation of the extrinsic apoptotic pathway via TRAIL affects the localization of Pus10. Here we test Pus10’s actions in cells under the effect of intrinsic apoptotic pathway compounds.Pus10 has also shown to have an effect on cell proliferation. Cells in which Pus10 has been depleted show increased growth rates. Here we investigate expression levels of proteins involved in cell cycle regulation, in both wild-type and Pus10-depleted cells, and attempt to compile a model of how Pus10 could be interacting with this system, using RNA-Seq, qPCR, and ChIP.SARS-CoV-2, also known as COVID-19, is a coronavirus that quickly became a pandemic in late 2019, early 2020. In the two years since then, the virus has undergone many mutations. By tracking the spread of these mutations at a national or regional level, by studying the spread pattern, we can make predictions, and possibly even prevent the next pandemic.

This research was designed to develop and evaluate an automated alternative to the Box-Jenkins method of forecasting. The study involved two major phases. The first phase was the formulation of an automated ARIMA method; the second was the combination of forecasts from the automated ARIMA with forecasts from two other automated methods, the Holt-Winters method and the Stepwise Autoregressive method. The development of the automated ARIMA, based on a decision criterion suggested by Akaike, borrows heavily from the work of Ang, Chuaa and Fatema. Seasonality and small data set handling were some of the modifications made to the original method to make it suitable for use with a broad range of time series. Forecasts were combined by means of both the simple average and a weighted averaging scheme. Empirical and generated data were employed to perform the forecasting evaluation. The 111 sets of empirical data came from the M-Competition. The twenty-one sets of generated data arose from ARIMA models that Box, Taio and Pack analyzed using the Box-Jenkins method. To compare the forecasting abilities of the Box-Jenkins and the automated ARIMA alone and in combination with the other two methods, two accuracy measures were used. These measures, which are free of magnitude bias, are the mean absolute percentage error (MAPE) and the median absolute percentage error (Md APE).

With recent developments in power conversion technologies and market trends that are driving those technologies toward further miniaturization and greater integration, the need for verifying an empirically based modeling methodology for proprietary power converters such as Power Supply on Chip (PwrSoC) products has risen significantly. This need motivates the investigation of black-box models which require little or no knowledge of the internal workings of a system, for those areas of industry adopting PwrSoC technology as a point-of-load solution. This thesis reports a black-box modeling method tailored to accommodate but not limited to the requirements of a specific commercially available PwrSoC technology.

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Enligt det antagna klimatmålet år 2017 ska Sverige vara utsläppsneutralt år 2045. Detta innebär att Sveriges utsläpp av växthusgaser måste minska kraftigt. I denna studie studeras utsläpp från olika sektorer och genom en tidsserieanalys prediceras framtida utsläppsvärden fram. Resultaten i studien visar att utsläppen förväntas minska inom samtliga studerade sektorer men att ytterligare forskning krävs för att veta om denna minskning är tillräcklig. Vidare är resultaten känsliga för förändringar, varför uppdaterade och kontinuerliga analyser rekommenderas.

Flavonoids are plant secondary metabolites that play diverse roles in plants and human health. These compounds in most part exist in the glucosylated form. Grapefruit accumulates high levels of glucosylated flavonoids. Plant secondary product glucosyltransferases (GTs) catalyze the glucosylation reaction, but due to low homology at both the nucleotide and amino acid sequence level of different GTs, it is not possible to ascribe function based on sequence only. The hypotheses that PGT clones 9 and 11 are plant secondary product GTs and are biochemically regulated were tested. PGT 9 has been cloned into Pichia pastoris using the pPICZA and pPICZAα vectors, expressed, enriched, and screened for GT activity with a variety of phenolic substrates. Initial screens show catechol, gentisic acid, vanillin, and p-hydroxylphenylacetic acid as potential substrates for the PGT 9 protein. PGT 11 has been successfully cloned into pPICZA for transformation into yeast, expression, and subsequent characterization.

A half hour comedy pilot about a guy that crowd sources his life.