Includes bibliographical references (leaves 87-90) / The objective of this project was to test the efficacy of using Extensible Markup Language (XML) - in particular the DocBook 5.0b5 schema - and Extensible Stylesheet Language Transformation (XSLT) to render electronic courseware that can be dynamically re-formatted according to a student's individual learning style. The text of a typical lesson was marked up in XML according to the DocBook schema, and several XSLT stylesheets were created to transform the XML document into different versions, each according to particular learning needs. These learning needs were drawn from the Felder-Silverman learning style model. The notes had links to trigger JavaScript functions that allowed the student to reformat the notes to produce different views of the lesson.

Haemagglutination is the recognised serologic technique for common (ABO & Rh) blood group antigen phenotyping with limitations; typing multi-transfused patients and non-invasive foetal blood group determination. Molecular technological advances in characterising the 30 blood group systems have also generated PCR based direct genotyping techniques. Their utility in routine blood banking practice is a rapidly evolving field. The study aims were (1) to establish PCR-SSP assays for KEL, FY and JK blood group genotyping, (2) to evaluate HEA BeadChipTM technology for SNPs detection of RHCc, RHEe, CO, DI, DO, FY, JK, KEL, LU, LW, MNS and SC and haemoglobinopathy S, against serology considering reproducibility, reliability, sensitivity and labour saving potential (3) to evaluate the specificity and sensitivity of TaqMan Real-Time PCR for NIPD of foetal RHD7, RHC, RHc, RHE and SRY status and (4) to establish Real-Time PCR assays and MGB TaqMan probes for 8 sets of gender-independent “Bi-allelic” Short Insertion/Deletion Polymorphisms (SIDPs) as internal assay controls confirming the presence of cell-free foetal DNA (cffDNA). The PCR-SSP results for KEL, FY and JK typing results showed complete concordance with serology for all samples except 1×JKa and 7×Fyb; discrepancies resolved by subsequent DNA sequencing. The HEA BeadChipTM microarray validation on gDNA (n=224) and 22 saliva samples, giving overall allele detection (ADR) and concordance rates (CoR) of >99.8% for the 24 alleles. The Fyx allele (Fyb/Fyx: 265C>T) frequency in Scottish donors (5.4%) was much higher than expected. Saliva-derived gDNA was less sensitive than buffy coat-derived gDNA; ADR 89.9% and 100% respectively. NIPD foetal blood group genotyping by Real-Time PCR of 51 alloimmunised pregnancies (n=104 samples, 12 to ≥40 weeks) with was 100% accurate for RHD7, RHC and RHE assays; 95.7% for RHc and 99% for SRY. The utility of Real-Time TaqMan assays for 8 selected SIDPs as paternal (foetal) markers, were assessed using gDNA, cell-free DNA (cfDNA) from 61 donors and 6 extended families and finally with cffDNA from 13 pregnancies. Based on these research findings, many of the molecular assays are now established in Aberdeen.

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Thesis (PhD) -- AUT University, 2007. / Includes bibliographical references. Also held in print (v, 215 leaves : ill. ; 30 cm.) in City Campus Theses Collection (T 573.154 NAR)

Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 2003. / "May 2002" Includes bibliographical references (leaves 229-251).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Thesis (M. Ed.)--Kutztown State College. / Source: Masters Abstracts International, Volume: 45-06, page: 3069. Typescript. Includes bibliographical references (leaf 44).

It was first discovered by L. and H. Hirszfeld (1919) that the races of mankind differ in the relative frequencies of the four classical blood groups. Since that time an enormous amount of information has been collected from all parts of the world. To-day it can be said that more is known about the geographical variations of the human blood group genes than is known in the case of any other genes whatsoever, whether plant or animal (Dobzhansky, 1941 ). The four original blood groups depend upon the presence or absence of two agglutinable substances, or agglutinogens, in the red blood corpuscles, associated with the presence or absence of corresponding agglutinins in the serum. The agglutinogens are usually denoted by the letters A and B, the agglutinins by the letters a and b. Red cells containing A are agglutinated by serum containing a; similarly, red cells containing B are agglutinated by serum containing b.