Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-10T14:30:31Z No. of bitstreams: 1 2015 - Alessandra de Lima Machado.pdf: 1487065 bytes, checksum: cd9c7f300bf5103dbe9de9f9244f7bbb (MD5) / Made available in DSpace on 2017-10-10T14:30:33Z (GMT). No. of bitstreams: 1 2015 - Alessandra de Lima Machado.pdf: 1487065 bytes, checksum: cd9c7f300bf5103dbe9de9f9244f7bbb (MD5) Previous issue date: 2015-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The evaluation of toxicity caused by aluminum (Al) trees used in urban forestry is of great importance for the development of seedlings and plantings in appropriate conditions. The tree species Bauhinia variegata L. is commonly used in urban afforestation, requiring knowledge of their nutritional requirements and conflicts related to the presence of trees on the streets, for example, interference in the electricity distribution networks. Pruning is the main practice management done on trees planted in the streets. Optimizing the pruning process can generate benefits such as less frequent pruning, lower cost and higher efficiency, especially for the energy sector. The objectives of this study were to evaluate the influence of Al in the development of Bauhinia variegata seedlings, and analyze the effectiveness of different types of pruning cut with or without the use of Al, in order to reduce shoots. This study was developed in two stages, resulting in two chapters. The first, directly related to mineral nutrition, with the study aimed to investigate the effects of Al toxicity on growth and root development. Experiments were performed in simple and complete nutrient solution and in acid soil substrate. The second chapter presents the study to assess the effectiveness of different types of pruning cut made in adult plants of Bauhinia variegata, with and without the use of Al as a growth inhibitor. The results indicated that this species is sensitive to the toxicity of Al from the concentration of 50 ?M, being observed decrease in root growth rate and relative root elongation. The critical dose to paralyze Al root growth were 151,48 ?M of Al in simple nutrient solution and 388,72 ?M of Al in complete nutrient solution. Al caused increase in the diameter of the roots of seedlings from the concentration at 400 ?M solution. The application of limestone in acid soil favored the growth of plants of Bauhinia variegata, resulting in higher dry matter weight values of root and shoot dry mass weight. When pruning was held close to the trunk and Al application shoots were not observed until the last evaluation at 210 days after pruning. In pruning held close to the trunk without Al application occurred shoots. In the cuttings leaving stump 20 and 40 cm, with or without the application of Al occurred shoots / A avalia??o da toxidez causada pelo alum?nio (Al) em ?rvores utilizadas na arboriza??o urbana ? de grande import?ncia para o desenvolvimento de mudas e plantios em condi??es adequadas. A esp?cie arb?rea Bauhinia variegata L., ? comumente utilizada na arboriza??o de urbana, sendo necess?rio o conhecimento das suas exig?ncias nutricionais e dos conflitos relacionados ? presen?a das ?rvores nas ruas, como, por exemplo, a interfer?ncia nas redes de distribui??o de energia el?trica. A poda ? a principal pr?tica de manejo realizada nas ?rvores plantadas nas ruas. A otimiza??o do processo de podas pode gerar benef?cios como menor frequ?ncia de podas, menor custo e maior efici?ncia, especialmente para o setor el?trico. Os objetivos deste trabalho foram avaliar a influ?ncia do Al no desenvolvimento de mudas de Bauhinia variegata, e analisar a efici?ncia de diferentes tipos de corte em poda associado ou n?o ao uso de Al, de modo a diminuir brota??es. Estes estudos foram desenvolvidos em duas etapas, resultando assim em dois cap?tulos. O primeiro, relacionado diretamente ? nutri??o mineral, com o estudo direcionado ? investiga??o dos efeitos da toxidez do Al no crescimento e desenvolvimento de ra?zes. Foram realizados experimentos em solu??o nutritiva simples e completa e em substrato de solo ?cido. O segundo cap?tulo apresenta o estudo de avalia??o da efici?ncia de diferentes tipos de corte em podas realizadas em plantas adultas de Bauhinia variegata, com e sem a utiliza??o de Al como inibidor de crescimento. Os resultados indicaram que esta esp?cie ? sens?vel ? toxidez do Al a partir da concentra??o de 50 ?M, sendo observada diminui??o na taxa de crescimento radicular e na elonga??o radicular relativa. As doses cr?tica de Al que paralisaria o crescimento radicular foram de 151,48 ?M de Al em solu??o nutritiva simples e de 388,72 ?M de Al em solu??o nutritiva completa. O Al provocou aumento do di?metro das ra?zes de mudas a partir da concentra??o em solu??o de 400 ?M. A aplica??o de calc?rio em solo ?cido favoreceu o crescimento de plantas de Bauhinia variegata, resultando em maiores valores de peso de massa seca de raiz e peso de massa seca de parte a?rea. Quando a poda foi realizada rente ao tronco principal e com aplica??o de Al n?o foram observadas brota??es at? a ?ltima avalia??o, aos 210 dias ap?s a poda. Na poda realizada rente ao tronco principal sem aplica??o de Al ocorreram brota??es. Nas podas deixando toco de 20 e 40 cm, com ou sem aplica??o de Al, ocorreram brota??es.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-11-07T11:43:17Z No. of bitstreams: 1 2011 - Luciana de Lima Bezerra.pdf: 823571 bytes, checksum: aeff9177e7ee978c9b6138f66f82aacc (MD5) / Made available in DSpace on 2017-11-07T11:43:17Z (GMT). No. of bitstreams: 1 2011 - Luciana de Lima Bezerra.pdf: 823571 bytes, checksum: aeff9177e7ee978c9b6138f66f82aacc (MD5) Previous issue date: 2011-02-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / This study aimed to evaluate the influence of babesiosis in embryo recovery rates, early pregnancy and embryonic loss. The project was carried out in a Central Commercial Embryo Transfer, located in the city of Itaguai, and two farms in the county of Serop?dica-RJ, using animals from the same breed (Mangalarga Marchador). The 13 donors and 40 recipients used in this experiment were positive for Theileria equi confirmed by nested-PCR method . Two embryo collections were performed in donor mares in two consecutive estrous cycles (GID), in sequence these same animals were treated with imidocarb dipropionate (1.2 mg / kg IM.) in order to conduct two more embryo collections in two estrous cycles (GIId). The embryo recipients were divided into two groups of 20 animals each, where one was the control group (IRG), and the other group was treated (GIIr) with 1.2 mg / kg IM imidocarb dipropionate, with the aim of evaluating pregnancy rate at 15, 30, 45 and 60 days. The embryo donors mares had normal erythrocyte before and after treatment with imidocarb dipropionate. ConcerningWBC, the embryo donors showed a decrease in the count of total leukocytes and neutrophils after treatment and a slight increase of lymphocytes and monocytes after treatment. The embryo recipients showed normal erythrocyte and leukocyte counts before and after the treatment with imidocarb dipropionate. After 52 embryo collections performed, embryo recovery rate was 53.84% (14/26) and 65.38% (17/26) ( p> 0,05) for GID and GIId respectively. The pregnancy rate was 70% (14/20) ( p> 0,05) at 15, 30, 45 and 60 days in the Gir and the GIIr was 85% (17/20) ( p> 0,05) at 15 days, 80% (16/20) ( p> 0,05) at 30 , 45 and 60 days. Treatment with imidocarb dipropionate did not improve significantly the reproductive efficiency in an ET program. / Este estudo teve por objetivo avaliar a influ?ncia da babesiose nas taxas de recupera??o embrion?ria, gesta??o e perda embrion?ria precoce. O projeto foi realizado em uma Central Comercial de Transfer?ncia de Embri?o, situado no Munic?pio de Itagua?, e em dois haras no munic?pio de Serop?dica- RJ. Foram utilizadas 13 doadoras e 40 receptoras de embri?o da ra?a Mangalarga Marchador, positivas para Theileria equi atrav?s do m?todo de nested-PCR. Nas ?guas doadoras foram realizados duas coletas de embri?es em dois ciclo estrais consecutivos (GId), em sequ?ncia, esses mesmos animais foram tratados com dipropionato de imidocarb (1,2 mg/kg IM.) para realiza??o de mais duas coletas de embri?es em dois ciclos estrais (GIId). As receptoras de embri?o foram divididas em dois grupos de 20 animais cada, onde um grupo foi o controle (GIr) e, o outro grupo, foi tratado (GIIr) com 1,2 mg/ Kg IM de dipropionato de imidocarb, com intuito de avaliar a taxa de gesta??o aos 15, 30, 45 e 60 dias. As ?guas doadoras apresentaram eritrograma normal antes e ap?s o tratamento com dipropionato de imidocarb, e em rela??o ao leucograma, apresentaram diminui??o na contagem de leuc?citos e neutr?filos totais e discreto aumento de linf?citos e mon?citos somente ap?s o tratamento. As receptoras apresentaram eritrograma e leucograma normal antes e ap?s o tratamento com dipropionato de imidocarb. Ap?s a realiza??o de 52 coletas de embri?o, a taxa de recupera??o embrion?ria foi 53,84% (14/26) e 65,38% (17/26) (p> 0,05) para GId e GIId respectivamente. A taxa de gesta??o foi de 70% (14/20) (p> 0,05) aos 15, 30, 45 e 60 dias no grupo GIr e para o GIIr foi 85% (17/20) (p> 0,05) aos 15 dias, 80% (16/20) (p>0,05) aos 30, 45 e 60 dias. O tratamento com dipropionato de imidocarb na forma como foi realizado n?o promoveu melhora significativa na efici?ncia reprodutiva em um programa de TE.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-01-25T12:42:16Z No. of bitstreams: 1 2007 - Marco Aur?lio Soares Pinheiro.pdf: 727221 bytes, checksum: 4a4a5210bea956397c8eb8b3c6ed9487 (MD5) / Made available in DSpace on 2018-01-25T12:42:16Z (GMT). No. of bitstreams: 1 2007 - Marco Aur?lio Soares Pinheiro.pdf: 727221 bytes, checksum: 4a4a5210bea956397c8eb8b3c6ed9487 (MD5) Previous issue date: 2008-08-30 / The present study was developed at Santuary of Silvester Life, Serra da Conc?rdia, Valen?a (RJ), aiming to collect informations which can subsidize the handling and the preservation of Euterpe edulis at the Atlantic Forest; to study the floristics and the structure of a secondary forest, which was submited to enrichment; to valuate the E. edulis development in a plantation of enrichment, and to confirm the viability of development of palm cabbage culture in impacted forestal remainings. Were used collecting of floristic and phytosociological facts in two parcels of 20x50m. It was estimated the viability of plantation of enrichment with E. edulis by analysing the growth in two parcels of 20x50m. It was established four classes of size of exposed stirps (C1= up to 0,5m; C2 from 0,5 to 1,5m; C3 from 1,3 to 3,0m and C4 from 3,0m on and with circumference at chest level (CAP) > 15cm). Each parcel was devided in ten subparcels of 10x10m, in which all palm cabbage plantation of (C4 class) had their CAP measurings and exposed etirps height taken.In each subparcel of 10x10m it was allocated a subparcel of 4,0x4,0m, where the individuals of the classes C1,C2 and C3 have had their measurings of diameter of colon, CAP and height of stirps taken. All palm cabbage were identified with aluminium plate printed in low relief and fixed with copper nails.The parcel 1 can be found at the bottom of the region nearby a stream, while the parcel 2 can be found almost 50m above the first parcel. It has been done two measurings in an interval of six months and, at the and of this period, it had been estimated the percentage of survival and of changing of class. The analyses of growth in each sample, and also between one another was done by the non parametric test of Kruskal-Wallis. The fragment was characterized by the index of similarity and diversity, by Margalef with some other seven remainings of Atlantic Forest with different degrees of impactation and distincts successional stages. It was also compared some abiotic characteristics between the fragments. The individuals of C1; C2 and C3 from parcel1 were significantly grown, speaking about the diameter of colon. The individuals of the same classes of parcel 2 have not had an expressive growth, but there have had a significative growth in height of exposed stirps for these classes. The C4 from parcel 1 were grown concerning to the CAP, but those one of the parcel 2 didn?t. Speaking about the height of stirps in both of the parcels, the growth was very significative. The percentage of survival were about 95,8% and 100% in the parcels 1 and 2, respectively. / O presente estudo foi desenvolvido no Santu?rio de Vida Silvestre, Serra da Conc?rdia, Valen?a (RJ), com o objetivo de coletar informa??es que possam subsidiar o manejo e a conserva??o de Euterpe edulis na Floresta Atl?ntica; estudar a flor?stica e a estrutura de uma floresta secund?ria submetida a enriquecimento; avaliar o desenvolvimento de E. edulis em plantio de enriquecimento e confirmar a viabilidade do desenvolvimento da cultura de palmito em remanescentes florestais impactados. Foram utilizados levantamentos flor?stico e fitossociol?gico em duas parcelas de 20x50m. Avaliou-se a viabilidade do plantio de enriquecimento com E. edulis atrav?s de an?lise de crescimento em duas parcelas de 20x50m. Foram estabelecidas quatro classes de tamanho de estipe exposta (C1=at? 0,5m; C2 de 0,5 a 1,5m; C3 de 1,3 a 3,0m e C4 acima de 3,0m e com circunfer?ncia a altura do peito (CAP) 15cm) Cada parcela foi dividida em dez subparcelas de 10x10m, onde todos os palmiteiros da classe C4 tiveram suas medidas de CAP e altura de estipe exposta tomadas. Em cada subparcela de 10x10m foi alocada uma subparcela de 4,0x4,0m, em que os indiv?duos das classes C1, C2 e C3 tiveram suas medidas de di?metro de colo, CAP e altura de estipe tomados. Todos os palmitos foram identificados com placas de alum?nio impressas em baixo relevo e afixadas com pregos de cobre. A parcela 1 se encontra em regi?o mais baixa, pr?xima ao c?rrego, enquanto que a parcela 2 se localiza cerca de 50m acima da primeira parcela. Foram feitas duas medi??es com intervalo de seis meses e, ao final deste per?odo, foram calculados os percentuais de sobreviv?ncia e de mudan?a de classe. A an?lise do crescimento em cada amostra, e tamb?m entre elas, foi feita atrav?s do teste n?o param?trico de Kruskal-Wallis. Caracterizou-se o fragmento atrav?s do ?ndice de similaridade e diversidade de Margalef com outros sete remanescentes de Mata Atl?ntica com diferentes graus de impacta??o e est?gios sucessionais distintos. Tamb?m foram comparadas algumas caracter?sticas abi?ticas entre os fragmentos. Os indiv?duos de C1, C2 e C3 da parcela 1 cresceram significativamente quanto ao di?metro de colo. Os indiv?duos das mesmas classes da parcela 2 n?o tiveram crescimento significativo, mas houve crescimento significativo em altura de estipe exposta para estas classes. Os C4 da parcela 1 cresceram quanto ao CAP, mas os da parcela 2, n?o. Quanto ? altura de estipe, em ambas as parcelas o crescimento foi significativo. Os percentuais de sobreviv?ncia foram de 95,8% e 100% nas parcelas 1 e 2, respectivamente.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-03-28T18:55:07Z No. of bitstreams: 1 2014 - Rodrigo Abreu de Oliveira Santos.pdf: 3274401 bytes, checksum: e90a6972ad50b4e274c0c019a74ee737 (MD5) / Made available in DSpace on 2018-03-28T18:55:07Z (GMT). No. of bitstreams: 1 2014 - Rodrigo Abreu de Oliveira Santos.pdf: 3274401 bytes, checksum: e90a6972ad50b4e274c0c019a74ee737 (MD5) Previous issue date: 2014-02-28 / CAPES / CNPq / Submerged structures composed of wood are deteriorated by the activity of molluscs and crustaceans borers. Among these, the teredinids (Mollusca, Bivalvia) are the most specialized marine borers, leading to huge economic losses. On the other hand, these wood-boring organisms are the most important decomposers agents of timber in estuarine regions. Comprehend the influence of environmental factors on the occurrence and dynamics of teredinids taxocenosis is critical to the zoological and ecological knowledge of the family. The present study was conducted in the estuary of Marambaia, Rio de Janeiro, RJ, aiming to conduct the survey of species of teredinids by using specialized test panels. The species distribution was correlated with levels of salinity along the estuary and monthly volumes of precipitation. Wood consumption was estimated by a novel method. Permanently flooded and periodically flooded regions (mangrove forests) were compared by the occurrence of teredinids and wood consumption, as well as test panels exposed to the environment for different periods of time. Five species of teredinids were identified: Bankia gouldi (Bartsch, 1908), Bankia fimbriatula (Mol; Roch, 1931), Lyrodus floridanus (Bartsch, 1922), Nototeredo knoxi (Bartsch, 1917) and Teredo furcifera (Linnaeus, 1758). The most abundant specie was B. fimbriatula (47.31%), followed by B. gouldi (41.15%). Only two individuals of N. knoxi were found. The nearest sea point and points on permanently flooded sites had higher numbers of species, higher abundances of individuals and higher rates of wood consumption. The different periods of exposure of the test panels to the environment did not change the configuration of teredinids? colonization, nor the rates of wood consumption. An inverse correlation was found between rainfall and the number of individuals throughout the experiment, but the effects of rainfall were felt belatedly by the organisms. No significant correlation between salinity and the distribution of the teredinids was found, however, the data pointed to the role of salinity as the determinant factor of the species? occurrence and of the taxocenosis? characteristics along the Marambaia estuary. / Estruturas submersas compostas por madeira s?o deterioradas pela atividade de moluscos e crust?ceos perfurantes. Entre estes, os teredos (Mollusca, Bivalvia) s?o os perfurantes marinhos mais especializados, causando enormes preju?zos econ?micos. Por outro lado, estes organismos s?o os mais importantes agentes decompositores de madeira em regi?es estuarinas. Compreender a influencia de fatores ambientais sobre a ocorr?ncia e din?mica da taxocenose de teredos ? fundamental para o conhecimento zool?gico e ecol?gico da fam?lia. O presente estudo foi realizado no estu?rio da Marambaia, Rio de Janeiro, RJ, com o objetivo de realizar o levantamento de esp?cies de teredo utilizando coletores especializados. A distribui??o das esp?cies foi correlacionada com os n?veis de salinidade ao longo do estu?rio e volumes mensais de precipita??o. O consumo de madeira foi estimado por um novo m?todo. Regi?es permanentemente alagadas e periodicamente alagadas (bosques de manguezal) foram comparadas quanto ? ocorr?ncia de teredos e consumo de madeira, assim como coletores expostos ao ambiente por diferentes per?odos de tempo. Cinco esp?cies de teredos foram identificadas: Bankia gouldi (Bartsch, 1908), Bankia fimbriatula (Moll; Roch, 1931), Lyrodus floridanus (Bartsch, 1922), Nototeredo knoxi (Bartsch, 1917) e Teredo furcifera (Linnaeus, 1758). A esp?cie mais abundante foi B. fimbriatula (47,31%), seguida de B. gouldi (41,15%). Somente dois indiv?duos de N. knoxi foram encontrados. O ponto mais pr?ximo do mar e os pontos em locais permanentemente alagados apresentaram maior ocorr?ncia de esp?cies, maiores abund?ncias de indiv?duos e de taxas de consumo de madeira. Os diferentes tempos de exposi??o dos coletores ao ambiente n?o alteraram a configura??o de coloniza??o dos teredos e nem o consumo de madeira. Foi encontrada uma correla??o inversa entre a precipita??o e o n?mero de indiv?duos ao longo do experimento, por?m os efeitos da precipita??o foram sentidos tardiamente pelos organismos. N?o foi encontrada uma correla??o expressiva entre a salinidade e a distribui??o dos teredos, por?m os dados apontam para seu papel como fator determinante da ocorr?ncia de esp?cies e das caracter?sticas da taxocenose ao longo do estu?rio da Marambaia.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-04-10T12:58:11Z No. of bitstreams: 1 2010 - Cla?dio Rocha Lopes.pdf: 2005134 bytes, checksum: 3f0410cad2ed7af0f6a0fb58a582090a (MD5) / Made available in DSpace on 2018-04-10T12:58:11Z (GMT). No. of bitstreams: 1 2010 - Cla?dio Rocha Lopes.pdf: 2005134 bytes, checksum: 3f0410cad2ed7af0f6a0fb58a582090a (MD5) Previous issue date: 2010-03-26 / The aim of this study was to characterize the main physical and chemical properties, and testing of combustion and CO2 reactivity of biomass, towards its use as material injetante the tuyeres of blast furnaces. This aims to use economic and environmental gains by reducing the rates of carbon emissions and trading of Certified Emission Reductions. The biomass studied were two types of cellulignin: cellulignin from 100% wood and 50% wood + 50% organic matter of trash- MOL, five types of waste biomass in natura: marc barley, macadamia shells, cobs corn, sugar cane bagasse and wood Eucalytus urophylla. Carbonization was carried out at three temperatures (450, 600 and 900 oC) to provide data that could determine the temperature or temperature range in which the ?in natura? biomass and cellulignin should be carbonized to obtain the best properties to use as fuel/reducer in blast furnaces. Simulation tests of injection of powdered materials in blast furnaces were performed with three different masses (200, 250 and 300mg) for setting the rate of injection, after setting the mass to be injected (250mg) studied the carbonaceous were tested to obtain rates of combustion (burning efficiency). Tests were also made of mixtures of biomass with coal of low volatile coal, showing the synergy when mixing these coals. The rates of combustion-IC is obtained from the analysis of gaseous combustion products by gas chromatography. Tests of reactivity to CO2 at three temperatures (950, 1000 and 1050 oC) in order to obtain kinetic data (apparent activation energy and frequency factor) of various coals. The continuous reaction model (Equation Mampel-1st order) was used and the kinetic values obtained from the Arrhenius plot. Correlations of various physical and chemical properties of carbonaceous study (total carbon, fixed carbon, volatile matter, alkali content, density-He, density-Hg, open porosity, total porosity and surface area) compared to the rate of combustion-IC were obtained. It was also correlated the rate of combustion-IC with the values of reactivity to CO2 / O objetivo geral deste trabalho foi avaliar a viabilidade de utiliza??o de biomassas e seus carv?es atrav?s da inje??o pelas ventaneiras dos Altos-Fornos - t?cnica conhecida como Inje??o de Carv?es Pulverizados (ICP). Desta forma, diferentes biomassas e seus carv?es tiveram as suas propriedades f?sicas e qu?micas avaliadas, sendo realizados tamb?m testes de combust?o e de reatividade ao CO2. Esta utiliza??o visa ganhos ambientais e econ?micos, atrav?s da redu??o dos ?ndices de emiss?es de carbono e comercializa??o de Certificados de Emiss?es Reduzidas. As biomassas estudadas foram dois tipos de celuligninas: celuligninas provenientes de 100% madeira e celuligninas provenientes de 50% madeira + 50% mat?ria org?nica do lixo, cinco tipos de res?duos de biomassas ?in natura?: baga?o de cevada, cascas de macad?mia, sabugo de milho, baga?o de cana e madeira de Eucalyptus urophylla. Foram realizadas carboniza??es em tr?s temperaturas (450?C, 600?C e 900?C), visando obter dados que pudessem determinar a temperatura ou faixa de temperatura na qual as celuligninas e biomassas ?in natura? devem ser carbonizadas para se obter as melhores propriedades visando a utiliza??o como combust?veis/redutor nos Altos-Fornos. Os carbon?ceos estudados foram ensaiados para a obten??o dos ?ndices de combust?o (efici?ncia de queima). Tamb?m foram realizados testes de misturas de carv?es de biomassas com carv?o mineral baixo vol?til, mostrando a sinergia quando se misturam estes carv?es. Foram realizados ensaios de reatividade ao CO2 em tr?s temperaturas (950?C, 1000?C e 1050?C) com o intuito de obter dados cin?ticos (energia de ativa??o aparente e fator de frequ?ncia) de diversos carv?es. Utilizouse o modelo de rea??o cont?nua (Equa??o de Mampel-1? ordem) e os valores cin?ticos foram obtidos atrav?s do gr?fico de Arrhenius. Correla??es de diversas propriedades f?sicas e qu?micas dos carbon?ceos estudados em rela??o ao ?ndice de combust?o-IC foram obtidas. Tamb?m foi correlacionado o ?ndice de combust?o-IC com os valores de reatividade ao CO2. Os resultados mostraram que as celuligninas CCC3(50% madeira + 50 % lixo) e CCC5 (100% madeira) possuem caracter?sticas semelhantes ao carv?o vegetal tradicional, podendo serem injetadas nas ventaneiras, sem necessidade de carboniza??o. A temperatura de carboniza??o de biomassas deve ser entre 450?C e 600?C, possibilitando melhores rendimentos gravim?tricos e boas caracter?sticas f?sico-qu?micas. De modo geral, os maiores ?ndices de combust?o foram obtidos pelos carv?es carbonizados ? 600?C e pelo Carv?o vegetal tradicional (madeira de Eucalyptus). Avaliando-se as propriedades f?sicas e qu?micas dos materiais carbon?ceos com o ?ndice de combust?o, pode-se dividir os materiais em quatro grupos espec?ficos (grupo de biomassas in natura, grupo das celuligninas, grupo dos carv?es vegetais e grupo dos carv?es minerais). Os valores dos ensaios de reatividade ao CO2 est?o em conson?ncia com os resultados de ?ndice de combust?o, onde os carv?es de celulignina, bem como os carv?es de biomassa tradicional, s?o materiais de alta reatividade com grande potencial para utiliza??o na inje??o em altoforno.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-04-11T16:32:51Z No. of bitstreams: 1 2010 - Jaci de Almeida.pdf: 1762363 bytes, checksum: 6b47b0e954a40b42efe066ff6875b0af (MD5) / Made available in DSpace on 2018-04-11T16:32:51Z (GMT). No. of bitstreams: 1 2010 - Jaci de Almeida.pdf: 1762363 bytes, checksum: 6b47b0e954a40b42efe066ff6875b0af (MD5) Previous issue date: 2010-12-10 / Ectoparasite affects in the animals, carrying great prejudices to the health and considerable economic losses. Antiparasitic utilization of wide action spectrum has been assisting in the losses reduction in consequence of these infestations. They were objectives of this work, evaluate of the treatment antiparasitic effects, in therapeutic doses, in the preventive control of the natural infestations for Dermatobia hominis and Haematobia irritans, and about the andrological characteristics, enzymatic profile (AST and ALT) and her correlations in Nelore bulls seminal caracteristics. They were used 20 bulls from 24 to 30 age months, managed in grazing system (Brachiaria decumbens and B. brizantha). The experiment was executed in completely randomized design with four treatments (T1 = Control, without medication; T2 = Ivermectina injet?vel to 3,15%; T3 = Fipronil ?pour-on? e T4 = Doramectina to 1%), three applications (0, 60 and 120 days), five repetitions (bulls) and five semen collections (D0, D15, D30, D45 and D60). The animals were submitted to andrological examinations biweekly inside the periods, being evaluated the ponderal, testicular and seminal characteristics, and the enzymatic profiles. In the statistical analyses were used Anova and comparisons between averages according to de Kruskal-Wallis e Bonferroni Tests in the GraphPad Prism? vers?o 4.0 to Windows?. The obtained results indicate that (a) the antiparasitic treatments used in the preventive control in the natural infestations of ectoparasites do not influence negatively the ponderal and reproductive characteristics, and (b) the enzymes analyses AST and ALT, in the sanguine serum, evidenced do not be efficient methodology for evaluation of Nelore bull spermatogenesis implication. The prophylactic application of the antiparasitic drugs, in the pre-breeding season, in bulls created extensive is an efficient technique of ectoparasitism natural control to D. hominis and H. irritans, being the product choice be entailed to the application practicability and to the cost in each region. / Os ectoparasitas afetam os animais, acarretando grandes preju?zos a sa?de e consider?veis perdas econ?micas. A utiliza??o de antiparasit?rios de amplo espectro de a??o tem auxiliado na redu??o de perdas em decorr?ncia dessas infesta??es. Foram objetivos desse trabalho, avaliar os efeitos dos tratamentos antiparasit?rios, em doses terap?uticas, no controle preventivo da infesta??o natural por Dermatobia hominis e Haematobia irritans, sobre as caracter?sticas androl?gicas, perfil enzim?tico (AST e ALT) e correla??es nas caracter?sticas seminais de touros Nelore. Foram utilizados 20 touros de 24 a 30 meses de idade, criados a pasto (Brachiaria decumbens e B. brizantha). O experimento foi executado em delineamento inteiramente casualizado com quatro tratamentos (T1 = Controle, sem medica??o; T2 = Ivermectina injet?vel a 3,15%; T3 = Fipronil ?pour-on? e T4 = Doramectina a 1%), tr?s aplica??es dos tratamentos (0, 60 e 120 dias), cinco repeti??es (touros) e cinco coletas de s?men (D0, D15, D30, D45 e D60). Os animais foram submetidos a exames androl?gicos quinzenais dentro dos per?odos, sendo avaliadas as caracter?sticas ponderais, testiculares, seminais e perfil enzim?tico. Nas an?lises estat?sticas foram utilizados Anova e compara??es entre m?dias de acordo com os testes de Kruskal-Wallis e Bonferroni no programa GraphPad Prism? vers?o 4.0 para Windows?. As an?lises dos resultados obtidos indicam que (a) os tratamentos antiparasit?rios empregados no controle preventivos na infesta??o natural de ectoparasitos n?o influenciam negativamente as caracter?sticas ponderais e reprodutivas, e (b) as an?lises das enzimas AST e ALT, no soro sangu?neo, evidenciaram n?o ser metodologia eficiente para avalia??o de comprometimento da espermatog?nese de reprodutores bovinos da ra?a Nelore. A aplica??o antiparasit?ria profil?tica, na pr?-esta??o de monta, em touros criados extensivamente ? uma t?cnica eficiente para o controle de ectoparasitismo natural por D. hominis e H. irritans, devendo a escolha do produto estar vinculada ? praticidade de aplica??o e ao custo em cada regi?o.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-04-12T13:33:43Z No. of bitstreams: 1 2017 - Meri Diana Strauss Foesch.pdf: 3588546 bytes, checksum: 6d8bf050ff816e1fbfc13bbbf1858b1f (MD5) / Made available in DSpace on 2018-04-12T13:33:43Z (GMT). No. of bitstreams: 1 2017 - Meri Diana Strauss Foesch.pdf: 3588546 bytes, checksum: 6d8bf050ff816e1fbfc13bbbf1858b1f (MD5) Previous issue date: 2017-02-22 / Mining is an economic activity that has many negative impacts on the environment. Water resources are the most affected by mining, the huge dams are built to store the tailings of the activity, and these, can contain leaks, infiltrations and even break. The rupture of the Fund?o dam in Mariana caused the greatest environmental disaster in Brazil. On November 5, 2015, the rejects stored in the dam are violently applied in the rivers downstream, in great concentration of the rivers Gualaxo do Norte and Carmo, until at Risoleta Neves and then down Rio Doce, until the Atlantic Ocean. This work, organized in two chapters, analyzed the physical-chemical characteristics of the water of the Gualaxo do Norte and Carmo Rivers in relation to Resolution 357/05 of CONAMA, after the dam disruption of Fund?o and related Sentinel-2A and Lansat-8. 36 physico-chemical samples were collected in six by six samples (without satellite scan day, or near data, and without the same points) in six months (April to September 2016), while The images were approved in April, July, August and September 2016 (as of May and June were discarded by the high cloud cover they presented). Random Forest and Linear Regression prediction methods were applied to satellite bands and spectral indices (NDWI, MNDWI, AWEIsh, AWEInsh, WRI, KT Wetness, NDVI). The results showed that the values of metals, color and turbidity and other variables of the polluted waters of the rivers polluted by the mining waste presented values higher than those allowed in CONAMA Resolution 357/05 and highly correlated with the spectral indices, the band 8 Sentinel ? 2A and Lansat-8 band 5. It was concluded that as images of the satellites Landsat 8 and Sentinel-2A, it can be used to estimate and monitor how the physico-chemical characteristics of the waters of the rivers affected by the rupture of the Fund?o dam, which can be used to verify if waters of other rivers Come to acquire these characteristics as a form of monitoring and other changes caused by the exploitation of ores in the springs / A minera??o ? uma atividade econ?mica que gera muitos impactos negativos no meio ambiente. Os recursos h?dricos s?o os mais expostos a sofrerem tais impactos em fun??o das imensas barragens que s?o constru?das para armazenar os rejeitos da atividade, e estas, podem conter vazamentos, infiltra??es e at? se romper. O rompimento da barragem de Fund?o, em Mariana, causou o maior desastre ambiental do Brasil. No dia 5 de novembro de 2015, os rejeitos armazenados na barragem atingiram violentamente os rios a sua jusante, em maior concentra??o os rios Gualaxo do Norte e do Carmo, at? a usina de Risoleta Neves e ap?s ela, o Rio Doce, at? o oceano Atl?ntico. Este trabalho, organizado em dois cap?tulos, analisou a situa??o das caracter?sticas f?sico-qu?micas da ?gua dos rios Gualaxo do Norte e do Carmo em rela??o a Resolu??o 357/05 do CONAMA, ap?s o rompimento da barragem de Fund?o, e relacionou com as caracter?sticas espectrais nos mesmos pontos usando imagens Sentinel-2 e Lansat-8, Foram coletadas trinta e seis amostras f?sico-qu?micas distribu?das em seis amostras por m?s (no dia da varredura do sat?lite, ou pr?ximo ? data, e sempre nos mesmos pontos) em seis meses (abril a setembro de 2016), enquanto as imagens utilizadas foram as de abril, julho, agosto e setembro de 2016 (as de maio e junho foram descartadas pela alta cobertura de nuvens que apresentaram). Foram analisadas a turbidez, cor e metais dissolvidos e suspensos da ?gua e utilizados os m?todos de predi??o por Random Forest e Regress?o Linear para com as bandas dos sat?lites e ?ndices espectrais (NDWI, MNDWI, AWEIsh, AWEInsh, WRI, K-T Wetness, FII, NDVI). Os resultados mostraram que os teores de metais, cor e turbidez e demais vari?veis das ?guas contaminadas dos rios polu?dos pelo rejeito de minera??o apresentaram valores acima dos permitidos na Resolu??o 357/05 CONAMA, e estiveram altamente correlacionadas com os ?ndices espectrais, a banda 8 Sentinel-2 e banda 5 Lansat-8. Concluiu-se que as imagens dos sat?lites Landsat 8 e Sentinel 2A, podem ser usadas para estimar e monitorar as caracter?sticas f?sico-qu?micas nas ?guas dos rios afetadas pelo rompimento da barragem de Fund?o, o que pode servir para verificar se ?guas de outros rios v?m adquirindo estas caracter?sticas, como forma de monitoramento e demais altera??es causadas pela explora??o de min?rios nos mananciais

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-17T18:34:58Z No. of bitstreams: 1 2016 - Christianne Perali.pdf: 1153381 bytes, checksum: d07ae3c36976ae3205f094d185e3fddf (MD5) / Made available in DSpace on 2018-05-17T18:34:58Z (GMT). No. of bitstreams: 1 2016 - Christianne Perali.pdf: 1153381 bytes, checksum: d07ae3c36976ae3205f094d185e3fddf (MD5) Previous issue date: 2016-03-29 / Brazilian pig farming is among the most important in the world. Brazil is always ranks as one of the leading exporters of this and other meats, as well as its important role in grain production. In the last decade, society in general has been increasingly concerned with the quality of food consumed not only by humans but also for animals that produce human food. Thus, mycotoxins have become a concern for all governments around the world, enacting restrictions and limits for the presence of these compounds in almost all food products, especially those from other countries. The scientific community has been searching for affordable and social and environmentally correct alternatives to minimize the adverse effects of these toxins. In this sense, the yeast cell wall (YCW), especially from Saccharomyces cerevisiae has been identified as one of the most promising alternatives. So, three experiments were developed to evaluate the ability of yeast cell wall in controlling the negative effects of three mycotoxins separately, aflatoxin B1 (AFB1), zearalenone (ZEA) and fumonisin B1 (FB1). Were evaluated: organic parameters (body weight, liver relative weight, kidneys relative weight, liver enzymes, urea and creatinine, among others), production parameters (weight gain, feed intake and feed conversion) and for AFB1 and ZEA also carcass quality parameters and meat quality. In Chapter 1 is presented a brief review of main issues inherent to the subject, as the production of Brazilian grain, Brazilian pork production, mycotoxins and their effects and, finally, the anti-mycotoxin additives and yeast cell wall. Chapter 2, 3 and 4 respectively presents the results of the above parameters obtained in each experiment that evaluated the PCL's ability to control the negative effects of AFB1 (Cap.2), ZEA (chapter 3) and FB1 (cap.4 ). After these evaluations, it was concluded that the studied PCL was able to control the negative effects of the three mycotoxins in the concentrations evaluated. / A suinocultura brasileira est? entre as mais importantes do mundo. O Brasil est? sempre figurando como um dos maiores exportadores mundiais desta e de outras carnes, al?m de seu important?ssimo papel na produ??o de gr?os. Na ?ltima d?cada, a sociedade de forma geral vem se preocupando cada vez mais com a qualidade dos alimentos consumidos n?o apenas pelo homem, mas tamb?m pelos animais que produzem o alimento humano. Assim, as micotoxinas passaram a ser uma preocupa??o de todos os governos ao redor do mundo, decretando restri??es e limites para a presen?a destes compostos em quase todos os produtos aliment?cios, em especial naqueles provenientes de outros pa?ses. A comunidade cient?fica vem buscando alternativas economicamente vi?veis e social e ambientalmente corretas para minimizar os efeitos nefastos destas toxinas. Neste sentido, a parede celular de leveduras (PCL), especialmente da Saccharomyces cerevisae vem sendo apontada como umas das alternativas mais promissoras, assim, no presente trabalho foram desenvolvidos tr?s experimentos objetivando avaliar a capacidade da parede celular de levedura em controlar os efeitos negativos de tr?s micotoxinas isoladamente, a saber, aflatoxina B1 (AFB1), zearalenona (ZEA) e fumonisina B1 (FB1), quando foram avaliados par?metros org?nicos (peso vivo, peso relativo do f?gado, peso relativo dos rins, enzimas hep?ticas, ur?ia e creatinina, entre outros), par?metros produtivos (ganho de peso, consumo de ra??o e convers?o alimentar) e, para AFB1 e ZEA tamb?m par?metros de qualidade de carca?a e de carne. No cap?tulo 1 est? apresentada uma breve revis?o de literatura que aborda os principais assuntos inerentes ao tema, como a produ??o de gr?os brasileira, situa??o da suinocultura nacional, as micotoxinas e seus efeitos e, finalmente, os aditivos anti-micotoxinas e a parede celular de leveduras. No cap?tulo 2, 3 e 4 est?o apresentados os resultados dos par?metros acima descritos obtidos no experimento que avaliou a capacidade da PCL em controlar os efeitos negativos respectivamente da AFB1 (cap.2), ZEA (cap.3) e FB1(cap.4). Ap?s estas avalia??es, concluiu-se que a PCL estudada foi capaz de controlar os efeitos negativos das tr?s micotoxinas estudadas, nas concentra??es avaliadas.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-17T19:12:34Z No. of bitstreams: 1 2016 - Thiago Andrade Bernini.pdf: 6680378 bytes, checksum: 98cf74e5c188b6420235be6f37868b6b (MD5) / Made available in DSpace on 2018-05-17T19:12:34Z (GMT). No. of bitstreams: 1 2016 - Thiago Andrade Bernini.pdf: 6680378 bytes, checksum: 98cf74e5c188b6420235be6f37868b6b (MD5) Previous issue date: 2016-02-26 / CAPES / The survey and analysis of the spatial distribution of soil attributes through geostatistics tools are essential for agricultural land use according to soil capability. The images of synthetic aperture radar (SAR) have great potential for soil moisture estimation and, thus, these sensors can assist in mapping the physical-hydric and physical properties of soils. The overall objective of this study was to evaluate the potential use of radar images (microwave) ALOS/PALSAR on the identification of soils in an area of the Botucatu formation, dominated by sandy and medium texture soils in the municipality of Mineiros, Goi?s State, Brazil. The area has approximately 946 hectares, with the relief of the region ranging from plain to low undulating hills and the geology of the area is composed basically by sandstones of the Botucatu formation. In the present study there were sampled 84 points for calibration and 25 points for validation, collected in the depths of 0-20 cm and 60-80 cm. The soil samples were analyzed for the determination of sand, silt, clay, field capacity (CC), permanent wilting point (PMP) and total water available (AD). For the development of the work were acquired ALOS/PALSAR radar images of five dates and different polarizations, totaling 14 images, which were processed for the geographic and radiometric corrections, using a DEM. Were also generated covariates of terrain attributes: high (ELEV), slope (DECLIV), relative position of the slope (PR-DECL), vertical distance of the drainage channel (DVCD), ls factor (FACTOR-LS) and Euclidean distance (D-EUCL). Prediction of soil attributes was performed using Random Forest methods (RF) and Random Forest Kriging (RFK), having as predictive covariates the radar imaging and terrain attributes. Image processing of the ALOS/PALSAR radar images enabled the geographical and radiometric corrections, transforming the data into backscatter coefficient (??) in units of dB, corrected by digital elevation model (MDE). The acquired images represented broad range of ?? between the different dates. The soils of the study area are predominantly sandy, with most of the sampled points classified as Neossolos Quartzar?nicos (Entisols), followed by Latossolos (Oxisols). The RF models employed for prediction of physical-hydric and physical attributes of soils provided an analysis of the contribution of these covariates in the predictive models. The landscape attributes that caused the largest impact in the prediction of the studied attributes are related to the altitude. The images of 5/3/2009 (HH1, VV1, HV1 and VH1) and 9/26/2010 (HH3 and HV3), obtained in drier periods, had best correlations with the soil attributes. The analysis of the semivariograms of the RF prediction models residues demonstrated greater spatial dependence in the 60 to 80 cm layer. The Kriging approach coupled with RF model contributed to the improvement of the prediction of sand, clay, CC and PMP. Using ALOS/PALSAR radar images and terrain attributes as covariates in RFK models showed potential to estimate the physical (sand and clay) and physical-hydric (CC and PMP) attributes, which can assist in mapping of soils associated with the Botucatu formation parent materials. / O levantamento e a an?lise da espacializa??o dos atributos do solo atrav?s de ferramentas de geoestat?stica s?o fundamentais para que cada hectare de terra seja cultivado segundo as suas reais aptid?es. As imagens de radar de abertura sint?tica (SAR) t?m um grande potencial para a estima??o de umidade do solo e, desta forma, estes sensores podem auxiliar no mapeamento de propriedades f?sicas e f?sico-h?dricas dos solos. O objetivo geral deste estudo foi avaliar o potencial de utiliza??o de imagens de radar (micro-ondas) ALOS/PALSAR na identifica??o de solos em uma ?rea da Forma??o Botucatu, dominada por solos de textura arenosa e m?dia no munic?pio de Mineiros - GO. A ?rea tem aproximadamente 946 ha, com o relevo da regi?o variando de plano a suave ondulado e geologia da ?rea ? composta basicamente, por Arenitos da Forma??o Botucatu. No presente estudo foram amostrados 84 pontos para calibra??o e 25 pontos para valida??o, coletados nas profundidades de 0-20 cm e 60-80 cm. As amostras de solo analisadas para a determina??o de areia, silte, argila, capacidade de campo (CC), ponto de murcha permanente (PMP) e ?gua total dispon?vel (AD). Para o desenvolvimento do trabalho foram adquiridas imagens de cinco datas e diferentes polariza??es, totalizando 14 imagens, que foram processadas para a corre??o geom?trica e corre??o radiom?trica, utilizando o MDE. Tamb?m foram gerados covari?veis dos atributos do terreno: eleva??o (ELEV), declividade (DECLIV), posi??o relativa da declividade (PR-DECL), dist?ncia vertical do canal de drenagem (DVCD), fator-ls (FATOR-LS) e dist?ncia euclidiana (D-EUCL). A predi??o dos atributos do solo foi realizada utilizando os m?todos Random Forest (RF) e Random Forest Krigagem (RFK), tendo como covari?veis preditoras as imagens de radar e os atributos do terreno. O processamento das imagens do radar ALOS/PALSAR possibilitou as corre??es geom?trica e radiom?trica, transformando os dados em unidades de coeficiente de retroespalhamento (??) corrigidos pelo modelo digital de eleva??o (MDE). As imagens adquiridas representaram de forma ampla as varia??es de ?? ocorridos em diferentes datas. Os solos da ?rea de estudo s?o predominantemente arenosos, com a maioria dos pontos amostrados classificados como NEOSSOLOS QUARTZAR?NICOS, seguidos dos LATOSSOLOS. Os modelos RF empregados para a predi??o dos atributos f?sicos e f?sico-h?dricos dos solos proporcionaram a an?lise da contribui??o das covari?veis preditoras. Os atributos do terreno que exerceram maior influ?ncia na predi??o dos atributos estudados est?o relacionados ? eleva??o. As imagens de 03/05/2009 (HH1, VV1, HV1 e VH1) e 26/09/2010 (HH3 e HV3), obtidas em per?odos mais secos, tiveram melhores correla??es com os atributos do solo. As an?lises dos semivariogramas dos res?duos da predi??o dos modelos RF demonstraram maior depend?ncia espacial na camada de 60 a 80 cm. A abordagem da Krigagem somada ao modelo RF contribu?ram para a melhoria da predi??o dos atributos areia, argila, CC e PMP. O uso de imagens de radar ALOS/PALSAR e atributos do terreno como covari?veis em modelos RFK mostrou potencial para estimar os atributos f?sicos (areia e argila) e f?sico-h?dricos (CC e PMP), que podem auxiliar no mapeamento de solos associados aos materiais de origem da Forma??o Botucatu.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-18T17:44:09Z No. of bitstreams: 1 2014 - Dayana da Silva Correia.pdf: 2038600 bytes, checksum: 031920d278558b902310d93f752920d7 (MD5) / Made available in DSpace on 2018-05-18T17:44:09Z (GMT). No. of bitstreams: 1 2014 - Dayana da Silva Correia.pdf: 2038600 bytes, checksum: 031920d278558b902310d93f752920d7 (MD5) Previous issue date: 2014-03-26 / CAPES / CNPq / The symbiosis between soil invertebrates and micro"organisms is a major ally in promoting the decomposition of plant residues in the soil. The micro"organisms in turn, have an immense genetic diversity and play crucial roles in maintaining ecosystems. One of these functions is the production of extracellular enzymes that assist the mineralization of organic matter. The possibility of developing new biotechnological processes based on the exploration of microbial diversity is immense, due to the great variability that exists between biological systems and it can be optimized to improve the agricultural production systems in a sustainable manner. The objective of this work was to study the profile of the bacterial community and cellulolytic potential of bacteria isolated from three different species of invertebrates saprophages. The experiments were performed at the Experimental Station of Embrapa Agrobiology, Serop?dica, Rio de Janeiro State. Millipede, of the Trigoniulus corallinus species, were collected in piles of plant compounds from local experimental field sites, which were subsequently incubated for 60 days under six different diets. Bacterial diversity in the intestinal tract of invertebrates was analyzed by PCR"DGGE of 16S rDNA gene amplification by PCR electrophoresis in denaturing gradient gel (DGGE); two domains were used, Bacteria and Actinobacteria. Some bands of the DGGE gel were extracted and sequenced. To assess the potential for production of cellulases in response to the presence of carboxy methyl cellulose (CMC) of isolates, the technique Congo red stain was used and the values were expressed as means (Ie) enzymatic index. From the highest values of (Ie) twenty" three bacteria were selected for the analysis of 16S rDNA. After the phylogenetic identification, the cellulolytic potential was rated, through cellulolytic activity of endoglucanase (CMCase), and endo" and exoglucanases (FPase) tests. To determine the molecular weight and activity of the enzymes polyacrylamide gels (SDS"PAGE) and zymography were performed. The results obtained in the technique of DGGE, the profiles of DGGE bands, showed that the intestinal microbiota of the invertebrates has distinct bacterial groups. It is possible to infer that, despite the communities having similar abundance, such as in the Trigoniulus corallinus and Cubaris murine species, the groups that make up this abundance were different among the invertebrate?s species. From the clones of the incised bands, three phyla members, Proteobacteria, Firmicutes and Bacteroidetes, were sequenced. Through phylogenetic analysis, it was possible to identify the 23 species of bacteria. Presenting two distinct Actinomycetes and Firmicutes phylum, the largest genus identified was Streptomyces, followed by an isolated Bacillus, Paenibacillus, and Staphylococcus. The intestinal tract of the three species of saprophages invertebrates showed to be an adequate environment for prospection of bacteria with cellulolytic efficiency, with high potential for future biotechnological studies. / A simbiose entre invertebrados do solo e microrganismos ? um grande aliado no auxilio da decomposi??o de res?duos vegetais presentes no solo. Os microrganismos por sua vez, apresentam uma imensa diversidade gen?tica e desempenham fun??es cruciais na manuten??o dos ecossistemas, uma dessas fun??es ? a produ??o de enzimas extracelulares que auxiliam na mineraliza??o da mat?ria org?nica. A possibilidade de desenvolver novos processos biotecnol?gicos com base na prospec??o da diversidade microbiana ? imensa, em decorr?ncia da grande variabilidade que existe entre os sistemas biol?gicos e que podem ser aperfei?oados para melhorar os sistemas de produ??o agr?colas de forma sustent?vel. O objetivo deste trabalho foi estudar o perfil da comunidade bacteriana e potencial celulol?tico de bact?rias isoladas de tr?s diferentes esp?cies de invertebrados sapr?fagos. Os experimentos foram montados no Campo Experimental da Embrapa Agrobiologia, Serop?dica, Rio de Janeiro. Gong?los, da esp?cie Trigoniulus corallinus, foram coletados em pilhas de compostos vegetais presentes em torno do campo experiemental, que posteriormente foram incubados durante 60 dias, sob seis diferentes dietas. A diversidade bacteriana do trato intestinal dos invertebrados foi analisada atrav?s da t?cnica de PCR"DGGE por amplifica??o do gene 16S rDNA PCR por eletroforese em gel com gradiente desnaturante (DGGE); foram utilizados dois dom?nios Bacteria e Actinobact?ria. Algumas bandas do gel de DGGE foram extra?das e sequenciadas. Para avaliar o potencial quanto ? produ??o de celulases em resposta ? presen?a de carboxi"metil"celulose (CMC) das bact?rias isoladas, foi utilizada a t?cnica de colora??o vermelho Congo, e os valores foram expressos atrav?s de (Ie) ?ndice enzim?tico. A partir dos maiores valores de (Ie), foram selecionadas vinte e tr?s bact?rias para a an?lise de sequenciamento do gene 16S rDNA. Ap?s a identifica??o filogen?tica, foi avaliado o potencial celulol?tico, atrav?s de testes de atividade celulol?tica de endoglucanases (CMCase) e endo e exoglucanases (FPase). Para determinar a massa molecular e atividade das enzimas foram realizados g?is de poliacrilamida (SDS"PAGE) e zimograma. Os resultados obtidos na t?cnica de DGGE, os perfis de bandas de DGGE mostrou que a microbiota intestinal dos invertebrados, det?m grupos bacterianos distintos. Pode"se inferir neste ponto, que apesar das comunidades possu?rem abund?ncia similar, como as esp?cies de Trigoniulus corallinus e Cubaris murina, os grupos que comp?em esta abund?ncia foram diferentes entre as esp?cies de invertebrados. A partir dos clones de bandas incisadas, foram sequ?nciados membros de tr?s filos, Proteobacteria, Firmicutes e Bacteroidetes. Atrav?s da an?lise filogen?tica, foi poss?vel identificar as 23 esp?cies de bact?rias. Apresentando dois filos distintos Actinomicetos e Firmicutes, o maior g?nero identificado foi Streptomyces, seguido de um isolado para Bacillus, Paenibacillus e Staphylococcus. O trato intestinal das tr?s esp?cies de invertebrados sapr?fagos revelou ser um ambiente h?bil ? prospec??o de bact?rias com efici?ncia celulol?tica, com alto potencial para futuros estudos biotecnol?gico.