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Efeito pr?-operat?rio da dexametasona e da metilprednisolona no controle da dor, edema e trismo ap?s cirurgia de terceiro molar: um ensaio cl?nico randomizado, triplo-cego, boca dividida. / Preemptive effect of dexamethasone and methilprednisolone on pain, swelling and trismus?after third molar surgery: a split-mouth randomized triple-blind clinical trialAlc?ntara, Carlos Eduardo Pinto de January 2012 (has links)
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Previous issue date: 2013 / O objetivo do presente estudo foi realizar uma avalia??o comparativa do efeito da dexametasona e da metilprednisolona no controle da dor, edema e limita??o da abertura bucal ap?s a extra??o de terceiros molares impactados. Dezesseis pacientes saud?veis (3 homens e 13 mulheres) com m?dia de 20,3 (DP= 1,25) anos de idade foram submetidos ? extra??o bilateral dos terceiros molares com intervalo de tr?s a quatro semanas entre as duas cirurgias. Uma hora antes de cada procedimento cir?rgico foi administrada em dose ?nica, por via oral, dexametasona 8 mg ou metilprednisolona 40 mg. Durante os intervalos p?s-operat?rios de 24, 48 e 72 horas e sete dias, o edema linear foi determinado utilizando medidas na face e o trismo atrav?s da abertura bucal m?xima. A dor p?s-operat?ria foi auto-registrada pelo paciente utilizando escala anal?gica visual durante as 72 horas iniciais, em intervalos de oito horas. A an?lise estat?stica envolveu an?lise descritiva, teste McNemar, Teste T pareado e Wilcoxon (p<0,05). A dexametasona apresentou melhor controle do edema em todos os per?odos p?s-operat?rios (p <0,02) e da abertura bucal dois dias ap?s a cirurgia (p = 0,029). Com rela??o ? dor n?o foi observada diferen?a estatisticamente significativa entre os medicamentos. A administra??o pr?-perat?ria de 8 mg de dexametasona apresentou melhor controle do edema e da limita??o da abertura bucal do que a metilprednisolona na dose de 40 mg, muito embora os medicamentos n?o diferiram no controle da dor. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The?aim of this study?was to make a comparative assessment of the effect of?dexamethasone and methylprednisolone?to control pain, swelling?and limitation?of mouth opening after extraction of?impacted third molars.?Sixteen healthy patients (3 men and 13 women)?with a mean age?of 20.3 (SD = 1,25) years underwent bilateral extraction?of?third molars with na interval of three to four weeks between the two surgeries. One hour?before each?surgical procedure?a single oral dose?of?dexamethasone 8?mg or methylprednisolone?40?mg was administered.?During the postoperative?intervals?of 24,?48?and 72?hours and?seven days, the swelling was determined using?linear?measures?in the?face and trismus?by?maximum mouth opening. The?postoperative pain?was self-recorded?by the patient using?visual analogue scale?in?72 hours with an interval of eight hours. Statistical analysis involved descriptive analysis, McNemar test, Wilcoxon?and paired T test (p?<0.05). Dexamethasone showed better control of swelling in all?postoperative periods (p?<0.02) and of mouth opening two days after surgery (p = 0.029). Regarding pain there was no statistically significant difference between the drugs. Preemptive dexamethasone?8 mg showed better control of swelling?and limitation?of mouth opening than methylprednisolone 40 mg, but there was no difference?in pain control.
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Comportamento de cuidado parental na popula??o de Sotalia guianensis na Ba?a de Sepetiba, RJ. / Behavior of parental care in Sotalia guianensis population in Sepetiba Bay, RJ.Correa, N?taly Fernandes 16 February 2012 (has links)
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Previous issue date: 2012-02-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior- CAPES / Previous studies in Sepetiba Bay, RJ, recorded calves and juveniles of Guiana dolphin through all
seasons. However few is know about the behaviors of parental care for this species. The main
objective of this work was to investigate the behavior of parental care in Sotalia guianensis
population of Sepetiba Bay. The film clips used in this study were recorded in 30 boat trips
conducted in the bay from December 2000 to August 2001. The evaluation of the parental care
degree was conducted using two measures: longitudinal care and transversal care. When the
mother located itself longitudinally ahead of its offspring (longitudinal position 1) or when it was
between the boat and the offspring (transversal position 1) the parental care were registered.
However, there was not care when the mother located itself behind its offspring (longitudinal
position 2) or when the mother left its offspring to be between the boat and it (transversal position
2). The influence of three variables was tested: group size, behavior state of the group (feeding
and traveling) and age class (calve and juvenile). From the total of 39,840 second of observation,
946 mother-calf dyads were recorded, and the results of the analyses of the kinds of care
conducted separately revealed that the longitudinal care was present in 84.7% of the time and the
transversal care in 57.7%. The time spent by the offspring on each longitudinal position (1 and 2)
was statistically different (U=58,033.50; p=0.02), receiving care during the median of 37
seconds. About the transversal care, there was not significant difference in the time that offspring
received care. The group size was statistically different when the mother located itself
longitudinally ahead of its offspring or not (U=151,525.50; p<0.01), the median of group size for
position 1 was 15 individuals, while for position 2 was 12. In relation to the mother position
between the boat or not, there was also significant variation (U=97,074.00; p=0.03), the median
of group size for transversal position 1 was 15 individuals and for position 2 was 14 animals.
Was identified significant influence of the behavior state of the group on longitudinal care, and
that care is intensely performed at feeding (N= 946; H3=17.60; p<0.01). The immatures of
Sepetiba Bay population have benefited from intense parental care, mainly during the feeding
activities and in large groups. The care is similarly offered to calves and juveniles, longitudinally
and transversally, suggesting a parent-offspring conflict of interest. / Estudos anteriores na Ba?a de Sepetiba, RJ, registraram a presen?a de filhotes e juvenis de
botos-cinzas (S. guianensis) durante todas as esta??es do ano, no entanto, pouco ? conhecido
sobre o comportamento de cuidado aos imaturos da esp?cie. Este trabalho objetivou investigar o
comportamento de cuidado parental da popula??o de botos-cinzas na Ba?a de Sepetiba. Foram
utilizados clipes das filmagens gravadas em 30 expedi??es conduzidas na ba?a entre dezembro de
2000 e agosto de 2001. A avalia??o do grau de cuidado parental foi conduzida utilizando duas
medidas: cuidado longitudinal e cuidado transversal. Quando a m?e se posicionava
longitudinalmente ? frente da prole (posi??o longitudinal 1) ou entre o barco e a prole (posi??o
transversal 1) foi registrado o cuidado parental. Entretanto, n?o havia cuidado quando a m?e se
localizava atr?s do imaturo (posi??o longitudinal 2) ou deixando o imaturo entre ela e a
embarca??o (posi??o transversal 2). Foi testada a influ?ncia de tr?s vari?veis no grau de cuidado
parental: tamanho de grupo, estado comportamental do grupo (pesca e deslocamento) e classe
et?ria da prole (filhote e juvenil). De um total 39.840 segundos de observa??o, foram registrados
946 pares de m?e e imaturo, e os resultados das an?lises dos tipos de cuidados conduzidas
separadamente revelaram que o cuidado longitudinal esteve presente em 84,7% do tempo e o
cuidado transversal em 57,7%. O tempo em que a prole esteve em cada posi??o longitudinal (1 e
2) foi estatisticamente diferente (U=58033,50; p=0,02), recendo cuidado da m?e durante a
mediana de 37 segundos. Em rela??o ao cuidado transversal, n?o houve diferen?a significativa no
tempo em que a prole recebia cuidado. O tamanho do grupo foi estatisticamente diferente quando
a m?e se posicionou longitudinalmente ? frente da prole ou n?o (U=151525,50; p<0,01), a
mediana para a posi??o 1 foi de 15 indiv?duos, enquanto na posi??o 2 de 12 indiv?duos. Houve
tamb?m varia??o significativa em rela??o ao posicionamento da m?e entre o barco ou n?o
(U=97074,00; p=0,03), a mediana na posi??o transversal 1 foi de 15 indiv?duos e na posi??o 2 de
14 indiv?duos. Foi encontrada influ?ncia significativa do estado comportamental do grupo apenas
sobre o cuidado longitudinal e tal cuidado ? intensamente realizado na pesca (N= 946; H3=17,60;
p<0,01). Na popula??o da Ba?a de Sepetiba, os imaturos se beneficiam de intenso cuidado
parental, principalmente durante as atividades de pesca e em grupos numerosos. O cuidado ?
investido de maneira similar aos filhotes e juvenis, tanto longitudinal quanto transversalmente,
sugerindo um conflito de interesses entre os pais e a prole.
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Caracteriza??o do metabolismo aer?bio e anaer?bio de Biomphalaria glabrata (Say, 1818) experimentalmente infectada por Echinostoma paraensei (Lie e Basch, 1967) / Metabolism characterization Aerobic and Anaerobic Biomphalaria glabrata Say, 1818 (Pulmonata, Planorbidae) Experimentally Infected With Different Doses of Miracidiais Echinostoma paraensei Lie and Basch, 1967Alves, Victor Menezes Tunholi 14 October 2015 (has links)
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Previous issue date: 2015-10-14 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Parasites belonging to the Echinostoma genus are characterized by having a complex
biological cycle, with two intermediate hosts and a final habitat restricted to the intestinal
lumen of the definitive hosts. These hosts include aquatic birds, mammals (including humans)
and occasionally some reptile and fish species. Their first intermediate hosts are freshwater
snails, into which the miracidia actively penetrate for development of the next three stages
(sporocysts, rediae and cercariae). Freshwater crustaceans, amphibians, fish and snails act as
the second intermediate hosts, enabling the formation of metacercariae, the infective larval
stage to the definitive host. In this work, Biomphalaria glabrata specimens were
experimentally infected with different doses (5 or 50) of E. paraensei miracidia. The snails
were dissected one, two, three and four weeks after infection to collect the hemolymph, shells
and tissues (gonad-digestive gland complex- GDG). In the hemolymph were quantified
glucose concentrations and carboxylic acids (succinic, pyruvic, lactic and oxalic acids), as
well as the activity of lactate dehydrogenase (LDH). The storage of tissues was measured
glycogen content and oxygen consumption (O2). Changes were observed in glycemia of the
snails, in both situations parasitism, with significant increase in glucose levels observed from
the third week post infection when compared to the control group. Changes have also been
described in relation to the activity of lactate dehydrogenase and are characterized by the
increase of its activity in the later periods of infection. In parallel, there was a decrease in
glycogen content in storage tissues, being such greater reduction in the digestive gland (larval
development site) in comparison to the cephalopedal mass. In addition, the infection by both
miracidiais doses resulted in increased levels of oxalic acid and lactic acid, as well as a
decline in the content of pyruvic and succinic acids in B. glabrata. The prepatent infection by
this equinostomatideo still significantly suppressed the phosphorylation state (state 3
respiration) and basal oxygen consumption (state 1 and 2) in B. glabrata, demonstrating that
infection by E. paraensei decreases the capacity of the intermediate host in performing
aerobic oxidative reactions. These results demonstrate the reduction in oxidative
decarboxylation rate of the reactions that are part of the tricarboxylic acid cycle and
acceleration of the process of anaerobic degradation of carbohydrates in the infected snails by
lactic acid fermentation, it is essential to ensure the obtaining of energy and the success of the
infection. Thus, the results observed in this study demonstrate that infection with five or 50
miracidia of E. paraensei caused significant metabolic changes in B. glabrata snails being
exposed to the largest load miracidial showed the greatest damage, featuring a dose-dependent
response / Parasitos pertencentes ao g?nero Echinostoma caracterizam por apresentar um ciclo
biol?gico complexo, com dois hospedeiros intermedi?rios e s?tio final de infec??o restrito ao
l?men intestinal de seus hospedeiros definitivos. Estes hospedeiros s?o representados
principalmente por aves aqu?ticas e semi-aqu?ticas, mam?feros, incluindo o homem, e
ocasionalmente algumas esp?cies de r?pteis e peixes. Possuem como primeiros hospedeiros
intermedi?rios moluscos l?mnicos, onde os mirac?dios penetram ativamente e desenvolvem at?
os est?gios de esporocistos, r?dias e cerc?rias. Por sua vez, crust?ceos, anf?bios, peixes e
moluscos l?mnicos atuam como segundos hospedeiros intermedi?rios onde ocorre a forma??o
de metacerc?rias, est?gios infectantes ao hospedeiro definitivo. Neste estudo, Biomphalaria
glabrata foi experimentalmente infectada com diferentes doses miracidiais (5 ou 50) de E.
paraensei. Os moluscos foram dissecados ap?s uma, duas, tr?s e quatro semanas de infec??o
para a coleta da hemolinfa e tecidos (complexo g?nada-gl?ndula digestiva- GGD e massa
cefalopediosa). Na hemolinfa foram quantificadas as concentra??es de glicose e de ?cidos
carbox?licos (succ?nico, pir?vico, l?tico e ox?lico), bem como a atividade da lactato
desidrogenase (LDH). Nos tecidos de estocagem foram mensurados os conte?dos de
glicog?nio e consumo de oxig?nio (O2). Altera??es foram observadas na glicemia dos
moluscos, em ambas as situa??es de parasitismo, com significativo aumento dos n?veis de
glicose verificado a partir da terceira semana de infec??o quando comparado ao grupo
controle. Mudan?as foram tamb?m descritas em rela??o ? atividade da lactato desidrogenase,
sendo caracterizadas pelo aumento de sua atividade nos per?odos mais tardios da infec??o. Em
paralelo, verificou-se um decr?scimo nos conte?dos de glicog?nio em tecidos de
armazenamento, sendo tal redu??o maior na gl?ndula digestiva (s?tio de desenvolvimento
larval), em compara??o ? massa cefalopediosa. A infec??o por ambas as doses miracidiais
ainda resultou em um aumento dos n?veis de ?cidos ox?lico e l?tico, bem como em um
decl?nio nos conte?dos de ?cidos pir?vico e succ?nico em B. glabrata. Significativa supress?o
no estado fosforilativo (estado 3 respirat?rio) e no consumo basal de oxig?nio (estado 1 e 2)
em B. glabrata infectada por E. paraensei foi demonstrada, indicando que a infec??o por este
equinostomat?deo diminui a capacidade do hospedeiro intermedi?rio em realizar rea??es
oxidativas aer?bias. Varia??es relevantes relacionadas ao estado mitocondrial desacoplado
(estado 3u) de B. glabrata infectada por tal tremat?deo foram tamb?m descritas. Tais
resultados demonstram redu??o na taxa de descarboxila??o oxidativa das rea??es que
integram o ciclo do ?cido tricarbox?lico e acelera??o do processo de degrada??o anaer?bia de
carboidratos nos moluscos infectados, atrav?s da fermenta??o l?tica, essencial para garantir a
obten??o de energia e o sucesso da infec??o. Assim, os resultados observados neste estudo
demonstram que a infec??o com cinco ou 50 mirac?dios de E. paraensei provocou
consider?veis altera??es metab?licas em B. glabrata, sendo que os moluscos expostos a maior
carga miracidial apresentaram os maiores danos, caracterizando uma resposta dosedependente.
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Ultrassonografia abdominal em peixe-boi amaz?nico (Trichechus inunguis): padroniza??o da t?cnica e achados ultrassonogr?ficos / Abdominal ultrasound in Amazonian manatee (Trichechus inunguis): standardized technique and sonographic findingsBarreto, Uiara Hanna Ara?jo 22 June 2015 (has links)
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Previous issue date: 2015-06-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The study of the wildlife has increased because many species find themselves threatened with extinction, the Amazonian manatee (Trichechus inunguis) is classified as vulnerable. The main factors that aggravate this situation is the slow reproduction of these animals, as well as poaching, which still occurs in the Amazon region, increased the need for preservation. The study of this kind is crucial to generate knowledge about the same, contributing in an attempt to reverse your current frame. Research on diagnostic imaging for manatees are rare, especially when directed ultrasound, it is an excellent technique to be carried out in wild animals, for not being invasive and provide real time information of each body. This abdominal ultrasound research will generate normal data, in the future we can diagnose diseases. The aim of this study was to standardize the examination technique and describe the abdominal ultrasound findings in inunguis Trichechus bred in captivity, increasing knowledge about the species and consequently aid in the diagnosis of diseases. The survey was conducted with 18 animals to describe the normal sonographic anatomy in the abdominal cavity of Amazonian manatee. During abdominal scan was viewed homogeneous liver, hyperechoic contoured edges and regular; the gallbladder showed anechoic content with regular and thin wall; stomach was present gas content, hypoechoic and thin wall. In the caudal abdomen visibilizou urinary bladder in piriformis format, bit full, hyperechoic wall and content ranging from anechoic and hypoechoic. Evaluated the subcutaneous tissue, which can differentiate the layer of skin, fat, muscle and peritoneum. The ultrasound examination obtained satisfactory results in this study, concluding that an efficient technique for evaluation in Amazonian manatee / O estudo com a fauna silvestre vem crescendo devido muitas esp?cies encontrarem-se amea?adas a extin??o, como o peixe-boi amaz?nico (Trichechus inunguis) que est? classificado como vulner?vel. Os principais fatores que agravam esse quadro ? a reprodu??o lenta destes animais, al?m da ca?a predat?ria, que ainda ocorre na regi?o amaz?nica, aumentando a necessidade de preserva??o. O estudo desta esp?cie ? fundamental para gerar conhecimento sobre a mesma, contribuindo na tentativa de reverter o seu quadro atual. Pesquisas sobre diagn?stico em imagem em peixe-boi s?o raras, principalmente quando direcionada a ultrassonografia, trata-se de uma excelente t?cnica para ser realizada em animais silvestres, por n?o ser invasiva e fornecer informa??es em tempo real de cada ?rg?o. Esta pesquisa de ultrassonografia abdominal ir? gerar dados de normalidade, para no futuro conseguirmos diagnosticar patologias. O objetivo do presente estudo foi padronizar a t?cnica de exame e descrever os achados de ultrassonografia abdominal em Trichechus inunguis criados em cativeiro, aumentando o conhecimento sobre a esp?cie e como consequ?ncia auxiliar no diagn?stico de patologias. A pesquisa foi realizada com 18 animais para descri??o da anatomia ultrassonogr?fica normal em cavidade abdominal de peixe-boi amaz?nico. Durante varredura abdominal foi visualizado f?gado homog?neo, hiperec?ico com contornos e bordos regulares; a ves?cula biliar apresentou conte?do anec?ico com parede regular e fina; no est?mago estava presente conte?do gasoso, parede hipoec?ica e fina. No abd?men caudal visibilizou a ves?cula urin?ria em formato piriforme, pouco repleta, parede hiperec?ica e conte?do variando entre anec?ico e hipoec?ico. Avaliou-se o tecido subcut?neo, onde pode-se diferenciar a camada de pele, gordura, muscular e perit?nio. O exame de ultrassonografia obteve resultados satisfat?rios neste estudo, concluindo ser uma t?cnica eficiente para avalia??o em peixe-boi amaz?nico
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Freq??ncia populacional de Dermatobia hominis (Linneaus Jr., 1781) (D?ptera: Cuterebridae) e sua correla??o com vari?veis clim?ticas em duas propriedades rurais no sudeste do Brasil / Frequency of population Dermatobia hominis and its correlation with variable climate in two rural properties in southeastern BrazilMedeiros, William Soares 31 July 2015 (has links)
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Previous issue date: 2015-07-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Dermatobia hominis (Linneaus Jr., 1781) (Diptera, Cuterebridae), popularly known in English as the Human Botfly or Warble fly, the only species of this genus and endemic to the neotropical region, is a holometabolous insect with an independent phase of life in its adult form and an obligatory parasitic phase in its larval form. In its parasitic phase, the larva is the cause of furuncular myiasis occurring in a large variety of endothermic animals, with most frequent occurrence in cattle, dogs and humans. The lack of information concerning dermatobiosis creates difficulties in its diagnosis. By means of a systematic review of the literature on the subject, it can be verified that this fly shows versatility in its phoresis and has a vast list of phoretic records. Field studies are restricted to observation of its larval form, because its adult form is difficult to observe, making this a rare event. With the intention of evaluating and comparing fluctuation in the population of D. hominis, searching for correlations between the incidence of Botfly larval infestations and climatic factors and their distribution on the body surface of cattle, two areas of study were selected, in which field collections were carried out twice monthly for counting the number of larvae in the herds. Discrepancies were discovered in the average number of larval nodes in cattle between these areas of study. These nodules showed variation in their bodily distribution on the cattle, with a higher incidence in the anterior region, compromising the quality of the animals? skins for economic purposes. In one of the areas, there was a correlation between the average number of larvae and the temperature and precipitation. These climatic factors are related to edaphic characteristics, influencing the penetration of the L3 larvae and the pupal period. For a greater understanding of the dynamics and fluctuations in the population of D. hominis in these areas, ample monitoring is needed to determine if the results encountered in this study are atypical or influenced by local characteristics. Currently, the demand for research is for methods of controlling the dermatobiosis, with emphasis on integrated plague management. Other deficiencies include following the Botfly?s complete life cycle in the laboratory and understanding certain peculiar adaptations, such as atrophied oral apparatus / A Dermatobia hominis (Linneaus Jr., 1781) (Diptera, Cuterebridae), popularmente conhecida como mosca do berne, ?nica esp?cie do g?nero e end?mica da regi?o Neotropical, ? um inseto holomet?bolo com uma fase de vida livre na sua forma adulta e uma fase parasit?ria obrigat?ria em sua forma larval. Em sua fase parasit?ria, a larva ? causadora da mi?ase furuncular ocorrendo em uma grande variedade de animais endot?rmicos, com ocorr?ncia mais frequente em bovinos, c?es e no homem. A falta de informa??o sobre a dermatobiose gera dificuldade em seu diagn?stico. Atrav?s de uma revis?o sistem?tica da literatura sobre o assunto, verifica-se que a mosca apresenta versatilidade para a foresia e conta com vasta lista de registros de for?ticos. Os estudos em campo se restringem a observa??es sobre sua forma larval, pois sua forma adulta ? de dif?cil observa??o tornando este evento raro. Visando avaliar e comparar a frequ?ncia populacional de D. hominis, buscando correla??o entre a incid?ncia do berne e fatores clim?ticos e a sua distribui??o na superf?cie corporal de bovinos, foram selecionadas duas ?reas de estudo, as quais foram realizadas coleta de campo quinzenalmente para o levantamento do n?mero de bernes no rebanho. Foram encontradas diverg?ncias no n?mero m?dio de n?dulos de berne em bovinos entre as ?reas de estudo. Os n?dulos apresentaram varia??o na distribui??o corporal dos bovinos, tendo maior incid?ncia na regi?o anterior, comprometendo a qualidade da pele do animal para fins econ?micos. Em uma das ?reas houve correla??o entre o n?mero m?dio de bernes e a temperatura e precipita??o. Esses fatores clim?ticos est?o relacionados ?s caracter?sticas ed?ficas, influenciando a penetra??o das larvas L3 e o per?odo pupal. Para o maior entendimento da din?mica e frequ?ncia populacional da D. hominis nessas ?reas, h? necessidade de amplo monitoramento para averiguar se os resultados encontrados neste estudo s?o at?picos ou influenciados por caracter?sticas locais. Atualmente a demanda de pesquisa est? em m?todos para controle da dermatobiose, com destaque para o manejo integrado de pragas. Outras lacunas est?o na realiza??o de seu ciclo completo em laborat?rio e compreens?o de adapta??es peculiares, tais como aparelho bucal atrofiado.
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Mecanismos envolvidos com as atividades antinociceptiva, antiedematog?nica e antiinflamat?ria do flavonoide majorit?rio das infloresc?ncias de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna) / Mechanisms underlying the antinociceptive, antiedematogenic, and anti-inflammatory activity of the main flavonoid from Kalanchoe pinnata flowers (Lam.) Pers. (fortune sheet)Ferreira, Raquel Teixeira 30 March 2016 (has links)
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Previous issue date: 2016-03-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Kalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study
investigated the antinociceptive and anti-inflammatory potential of the KP flower aqueous
extract (KPFE), the ethyl acetate (EtOAcF), butanol (BuOHF) and aqueous (AqF) fractions
and the main flavonoid [quercetin 3-O-?-L-arabinopyranosyl (1??) ?-L-rhamnopyranoside]
(KPFV) of KP, as well as possible mechanisms of action. Swiss albino mice, male (25-35g)
were pretreated with KPFE (30-300 mg/kg, s.c.) producing dose-related inhibition of acetic
acid-induced writhing (ID50 164.8 mg/kg), and the subcutaneous administration of KPFE (300
mg/kg), EtOAcF (12 mg/kg), BuOHF (15 mg/kg) or AqF (210 mg/kg) reduced leukocyte
migration on carrageenan-induced pleurisy in mice at 56.1%, 47.3%, 39.6% and 43.1%,
respectively. At doses of up to 240 times smaller than KPFE, KPFV (0.3-3.0 mg/kg, s.c.), also
inhibited leukocyte migration (ID50 1.78 mg/kg). In croton oil-induced ear edema in mice,
KPFE (3-30 mg/kg, s.c.) and KPFV (0.3-3.0 mg/kg, s.c.) presented dose-related antiedematogenic
activity (KPFE - ID50 4.3 mg/kg, KPFV - ID50 0.76 mg/kg). Treatment with
KPFE reduced the MPO and TNF-? concentration in the pleural exudates on carrageenaninduced
pleurisy test, while KPFV inhibited both isoforms of cyclooxygenase (COX), with
IC50 3.8 x 10-5 M (22.1 ?g/mL) for COX-1 and a maximum COX-2 inhibition of 43.5% (IC50
>8.4 x10-5M or 50 ?g/mL). The selectivity index (COX-1 IC50/COX-2 IC50) was <0.44. These
results indicate that KPFE and KPFV produced anti-inflammatory and antinociceptive activity
through the COX inhibition and TNF-? reduction. This study reveals that the main flavonoid
in KP flowers and leaves plays an important role in the ethnomedicinal use of this species for
healing inflammatory processes / Kalanchoe pinnata (KP) ? popularmente utilizada para tratamento de doen?as inflamat?rias.
Este estudo investigou o potencial antinociceptivo e anti-inflamat?rio do extrato aquoso das
infloresc?ncias de KP (EAFL), a fra??o acetato de etila (FAcOEt), a fra??o butan?lica
(FBuOH), a fra??o aquosa (FAq) e o flavonoide majorit?rio [quercetina 3-O-?-Larabinopiranosil
(1??) ?-L-ramnopiranos?deo] (KPFV) de KP, assim como poss?veis
mecanismos de a??o. Camundongos albinos, machos (25-35g) foram pr?-tratados com EAFL
(30-300 mg/kg, s.c.), produzindo inibi??o dose dependente das contor??es induzidas por ?cido
ac?tico (DI50 164,8 mg/kg), e a administra??o subcut?nea de EAFL (300 mg/kg), FAcOEt (12
mg/kg) ou FBuOH (15 mg/kg) reduziu a migra??o leucocit?ria na pleurisia induzida por
carragenina em camundongos em 56,1%, 47,3%, 39,6% e 43,1%, respectivamente. Em doses
at? 240 vezes menores que o EAFL, KPFV (0,3-3,0 mg/kg, s.c.), tamb?m foi capaz de inibir a
migra??o leucocit?ria (DI50 1,78 mg/kg). No teste do edema de orelha induzido por ?leo de
cr?ton em camundongos, EAFL (3-30 mg/kg, s.c.) e KPFV (0,3-3,0 mg/kg, s.c.) apresentaram
atividade antiedematog?nica dose dependente (EAFL - DI50 4,3 mg/kg, KPFV - DI50 0,76
mg/kg). O tratamento com EAFL reduziu a concentra??o de MPO e de TNF-? no exsudato
pleural obtido do teste da pleurisia induzida por carragenina, enquanto o KPFV inibiu ambas
as isoformas de cicloxigenase (COX), com CI50 3,8 x 10-5M (22,1 ?g/mL) para COX-1 e um
m?ximo de inibi??o da COX-2 de 43,5% (CI50 >8,4 x10-5M ou 50 ?g/mL). O ?ndice de
seletividade (COX-1 CI50/COX-2 CI50) foi < 0,44. Estes resultados indicam que EAFL e
KPFV produziram atividade anti-inflamat?ria e antinociceptiva envolvendo a inibi??o da
COX e a redu??o de TNF-?. Este estudo revela que o principal flavonoide presente nas
infloresc?ncias e folhas de KP possui papel importante no uso etnomedicinal desta esp?cie
nos processos de natureza inflamat?ria
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Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus / Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus.Gaud?ncio, Fabr?cio Nascimento 22 February 2016 (has links)
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Previous issue date: 2016-02-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The objective of this research was to evaluate the effect of the arthropod growth regulator fluazuron in the energetic metabolism of Rhipicephalus microplus. 6 stabled cattle divided into control and treated groups containing three animals each. A commercial formulation containing the active compound fluazuron was administered topically in the treated group. The engorged female ticks were collected from some days before (day 0) and after treatment of animals (4, 8, and 15 days). After collecting the engorged females, the following biological parameters were analyzed: weight of the engorged female, weight of the female after oviposition, laying period, laying weight, egg production rate, reproductive efficiency index, percentage of hatching and nutritional index. It was also evaluated the metabolic profile: in the hemolymph (concentration of glucose, total protein and organic acids - oxalic, pyruvic and lactic, enzymatic activity of LDH, ALT and AST); in the fat body (glycogen concentration and percentage of variation of neutral lipids); in eggs (concentration of glucose and total protein content, percentage change of neutral lipids and enzymatic activities of ALT and AST); and in the cuticle of engorged female ticks (percentage change of neutral lipids). Secondly, female immersion test was conducted to assess the activities of carboxylesterases enzymes, monooxygenases (MFO) and glutathione-S-transferase (GST) in sensitive strain of eggs and larvae (Porto Alegre, RS) compared with resistant strain (Jaguar, RS). It also assessed the detoxification enzymes in the fat body of engorged females of sensitive strain. The results showed that all the biological parameters showed lower results when compared to the control group after the exposure to fluazuron, except the weight of the female after oviposition. Although an increase was observed in the concentration of glucose in hemolymph on the 8th day and glycogen in the fat body in 15th day, there was no significant difference in the comparison of means between control and treated day-to-day. The concentration of lactic acid gradually increased in the treated group over time due to treatment, indicating increased anaerobic metabolism. There was no significant difference in the percentage change of neutral lipids in fat body, cuticle and eggs in any of the time intervals. No significant difference was observed in the activity of transaminases in the hemolymph, but it was observed in eggs after the treatment on the 8th day. The activity of MFO was higher in the comparison between the resistant and sensitive strains in eggs, but in larvae, the increase was observed in carboxylesterases in the resistant strain. In females, MFO showed high activity in the fat body as a function of fluazuron exposure, especially on day 4 in the treated group. Increases in transaminase activity and detoxification enzymes in females and eggs were associated with possible cell damage and trying to metabolism of active principle-tested. For the first time, a research about the metabolic effects of fluazuron has been conducted, focusing on different biochemical aspects, demonstrating that its effectiveness is related to a number of metabolic changes, leading to a parasite homeostasis breaking. / O objetivo desta pesquisa foi avaliar os perfis metab?lico-energ?tico de carrapatos Rhipicephalus microplus expostos ao fluazuron. 6 bovinos foram estabulados e divididos em grupos controle e tratado, contendo 3 animais cada. Uma formula??o comercial contendo o princ?pio-ativo fluazuron foi administrada por via t?pica nos animais do grupo tratado. F?meas ingurgitadas foram coletadas em dias determinados: antes (dia 0) e ap?s o tratamento dos animais (4, 8 e 15 dias). Ap?s a coleta das f?meas ingurgitadas, foram analisados os seguintes par?metros biol?gicos: peso da f?mea ingurgitada, peso da quen?gina, per?odo de postura, peso da postura, ?ndice de produ??o de ovos, efici?ncia reprodutiva, percentual de eclos?o e ?ndice nutricional. Foi avaliado tamb?m o perfil metab?lico: na hemolinfa (concentra??o de glicose, prote?nas totais e ?cidos org?nicos - ox?lico, pir?vico e l?tico, atividade enzim?tica de LDH, ALT e AST), no corpo gorduroso (concentra??o de glicog?nio e percentual de varia??o de lip?dios neutros), nos ovos (concentra??o de glicose, prote?nas totais, percentual varia??o de lip?dios neutros e atividade enzim?tica de ALT e AST), e na cut?cula das f?meas ingurgitadas (percentual de varia??o de lip?dios neutros). Num segundo momento, realizou-se teste de imers?o de f?meas ingurgitadas para posterior avalia??o das atividades das enzimas carboxilesterases, monooxigenases (MFO) e glutationa-S-transferase (GST) em ovos e larvas de diferentes cepas: sens?vel (Porto Alegre, RS) e cepa resistente (Jaguar, RS) ao fluazuron. Avaliou-se tamb?m as enzimas de detoxifica??o no corpo gorduroso de f?meas ingurgitadas de cepa sens?vel. Na an?lise dos resultados, todos os par?metros apresentaram resultados inferiores, quando comparados ao grupo controle, em fun??o da exposi??o ao fluazuron, exceto o peso da quen?gina. Embora tenha sido observado um aumento na concentra??o de glicose hemolinf?tica no 8? dia e glicog?nio no corpo gorduroso no 15?dia, n?o houve diferen?a significativa na compara??o das m?dias entre controle e tratado dia-a-dia. A concentra??o do ?cido l?tico apresentou aumento progressivo significativo no grupo tratado ao longo do tempo em fun??o do tratamento, indicando aumento do metabolismo anaer?bio. N?o houve diferen?a significativa para as varia??es percentuais de lip?dios neutros no corpo gorduroso, cut?cula e ovos em nenhum dos dias de avalia??o. Embora n?o tenha sido observada diferen?a significativa nas atividades das transaminases na hemolinfa, observou-se aumento significativo nos ovos ap?s o tratamento no 8?dia. A atividade da MFO foi maior, na compara??o entre as cepas resistente e sens?vel, nos ovos, e com rela??o ?s larvas; o aumento observado foi nas carboxilesterases da cepa resistente. Nas f?meas, a MFO apresentou atividade elevada no corpo gorduroso em fun??o da exposi??o ao fluazuron, principalmente no 4?dia no grupo tratado. Os aumentos na atividade das transaminases e das enzimas de detoxifica??o nas f?meas ingurgitadas e nos ovos foram associados a poss?veis danos celulares e ? tentativa de metaboliza??o do princ?pio-ativo testado. Pela primeira vez, foi realizado um estudo acerca dos efeitos metab?licos do fluazuron, enfocando diferentes aspectos bioqu?micos, evidenciando que a sua efic?cia est? relacionada a diversas altera??es metab?licas, levando a uma quebra da homeostase do carrapato
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Taxonomia dos Metazo?rios Ectoparasitos do Caparari Pseudoplatystoma fasciatum (LINNAEUS, 1766) (Siluriformes, Pimelodidae) do alto rio Xingu, Estado do Par?, Brasil / Taxonomy of the Metazoan Ectoparasites of Caparari Pseudoplatystoma fasciatum (LINNAEUS, 1766) (Siluriformes, Pimelodidae) from high Xingu river, Par? State, North BrazilAlbuquerque, F?bio Edir Amaral 29 March 2012 (has links)
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Previous issue date: 2012-03-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / A total of 25 specimens Caparari Pseudoplatystoma fasciatum were collected in four points of
the high Xingu river sector, between April 2010 and July 2011. Two new locality records of
Argulidae crustaceans are presented for Argulus pestifer and Dolops discoidalis. A new
monogenetic specie was proposed, registered and studied in details. Demidospermus n. sp.
differs from the other congenerics, because the anchors exhibit folds, variable forms, and deep
roots not defined. Demidospermus sp. n. also exhibits a thickened ventral bar, straight or
slightly curved anteriorly, with the extended Extremities, and with the Presence or Absence of
an above-median keel. The dorsal bar is variable in form, straight and slender, with Presence
or Absence of an above-median depression and tapered Extremities. The hooks are different
in size and form, the first and second pairs have the tip and the shaft lightly curved,
protuberant and truncated thumb, and the rod is dilated and tapered in proximal region. Hooks
different in size and shape, 1-2 pairs: blade point and slightly curved or curved, thumb
protruding, truncate, dilated or slender rod, tapered proximally, showing or not bulbs at the
proximal end; the pairs 3-4-6-7: point slightly bent, curved or straight blade almost straight or
moderately curved, thumb truncated or depressed, moderately dilated or slender rod,
presented a bulb at the proximal end; The male copulatory organ (MCO) is variable in shape,
with a convolute tube in the counterclockwise, and flaps that connect themselves on a
sclerotized edge. Male copulatory organ (MCO), with variable shape, like a coiled tube,
varying the number of rings in a counterclockwise direction, with tabs that connect to a
margin esclerotizada or G-shaped, with a ring in order counterclockwise, showing a dilated
base margin esclerotizada developed, from which protrudes a flap. The accessory piece, with
variable shape, slightly concave or concave, the distal tapered, curved or slightly curved,
serving as a guide to the MCO, the presence or absence of spines on the proximal end. The
vagina has a bag-shaped vestibule connected or not to the vaginal channel through an
annulated narrow. The study will can contribute to future research, in order to improve our
knowledge about the parasite fauna of aquatic organisms in the Amazon region. / Um total de 25 esp?cimes de Caparari P. fasciatum foram coletados no alto rio Xingu, entre
Abril de 2010 ? Julho de 2011. Foram apresentados dois novos registros de localidade de
crust?ceos parasitos da fam?lia Argulidae, foram registrados, Argulus pestifer e Dolops
discoidalis, e uma esp?cie de monogen?tico foi proposta como nova, examinada, registrada e
comentada em detalhes. Demidospermus sp. n. difere dos seus cong?neres por apresentar
?ncoras em formas vari?veis, ra?zes profundas n?o definidas, apresentando uma dobra. Barra
ventral em forma vari?vel, grossa, levemente curvada para a regi?o anterior, extremidades
alargadas, com presen?a ou n?o de uma quilha na parte antero-medial. Barra dorsal, em forma
vari?vel, reta e delgada, apresentando ou n?o uma depress?o antero-medial com extremidades
afiladas. Ganchos diferentes em tamanho e forma, pares 1-2: ponta e l?mina levemente curva
ou curvada, polegar protuberante, truncado, haste dilatada ou delgada, afilada proximalmente,
apresentando ou n?o bulbos na extremidade proximal; pares 3-4-6-7: ponta levemente
curvada, curva ou reta, l?mina quase retas ou moderadamente curvadas, polegar truncado ou
deprimido, haste moderadamente dilatada ou delgada, apresentado um bulbo na extremidade
proximal; par 5: ponta e l?mina retas, polegar achatado, haste alongada e fina, apresentando
um bulbo na regi?o proximal. ?rg?o copulat?rio masculino (OCM), apresentando forma
vari?vel, como um tubo enrolado, variando no n?mero de an?is no sentido anti-hor?rio,
apresentando abas que se conectam a uma margem esclerotizada ou em forma de G ao
contr?rio, com um anel em sentido anti-hor?rio, base dilatada apresentando uma margem
esclerotizada desenvolvida, de onde se projeta um flap. Pe?a acess?ria, apresentando forma
vari?vel, ligeiramente c?ncava ou c?ncava, parte distal afilada, curvada ou pouco curva,
servindo de guia para o OCM, presen?a ou n?o de espinhos na extremidade proximal. Vagina
apresentando um vest?bulo saculiforme, ligado ao canal vaginal atrav?s de um estreitamento
anelado presente ou n?o. O presente estudo poder? colaborar com futuras pesquisas, com o
prop?sito de melhorar o nosso conhecimento sobre a fauna dos organismos aqu?ticos da
regi?o Amaz?nica
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Caracteriza??o fenogenot?pica da resist?ncia antimicrobiana em Staphylococcus spp. isolados de mastites cl?nicas e subcl?nicas em unidades leiteiras de munic?pios do Rio de Janeiro como subs?dio para implementa??o de medidas de controle / Fenogenot?pica Characterization of antimicrobial resistance in Staphylococcus spp. isolated from clinical or subclinical mastitis in dairy units of municipalities of Rio de Janeiro as a subsidy for implementation of control measures.Mendon?a, Elaine Concei??o Liporage de 16 March 2012 (has links)
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Previous issue date: 2012-03-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The use of antibiotic in the control of intramammary infections and in the elimination of
its possible sources in dairy farms is an important control measure. However, the
inappropriate use of antibiotics can result in the appearance of resistant strains and
compromise the efficiency of the treatment. Besides Staphylococcus spp. are among the
main pathogens of bovine mastitis, they are often resistant to antibiotics, especially
beta-lactamics, mainly by two distinct mechanisms: the production of extracellular
enzyme beta-lactamase, encoded by the blaZ gene, and production of PBP2a or PBP2 '
a penicillin-binding protein with low affinity, encoded by the mecA gene. The
expression of mecA gene is constitutive or induced by beta-lactamic antibiotics, such as
oxacillin and cefoxitin. The mecA gene is inserted into the chromosome through a
staphylococcal mobile genetic element, called staphylococcal cassette chromosome mec
(SCCmec). The present study evaluated the phenogenotypical resistance profile to betalactam
antibiotics of 250 Staphylococcus spp. isolates, using oxacillin and cefoxitin as
markers in order to produce data to the knowledge of resistance in dairy farms located
in the South-Fluminense and the Metropolitan regions of the State of Rio de Janeiro to
support the implementation of measures to control this disease. The assessment of
resistance was made through 8 different phenotypic tests and yielded 54 profiles. Disk
diffusion and agar screen with oxacillin were used as "gold standard" for the calculation
of sensitivity, specificity and prediction once they are recommended by the CLSI
veterinarian as standardized tests. Disk diffusion with cefoxitin achieved the best
performance in the prediction of oxacillin resistance. Genotypic detection of mecA do
not provided any positive isolate, otherwise mecI and mecRI genes were also detected in
11.6% (29/250) of the studied Staphylococcus spp. Four cassette mec types were
detected (I, II, III and IV), being type I the most disseminated one. Gene blaZ was
detected in 5.2% (13/250) isolates. From these 13 blaZ positive isolates, the whole
system comprising blaR1-blaI-blaZ was detected in 23.1% (3/13) isolates / MENDON?A, Elaine Concei??o Liporage. Caracteriza??o fenogenot?pica da
resist?ncia antimicrobiana em Staphylococcus spp. isolados de mastites cl?nicas e
subcl?nicas em unidades leiteiras de munic?pios do Rio de Janeiro como subs?dio
para implementa??o de medidas de controle. 89 p. Disserta??o (Mestrado em
Ci?ncias Veterin?rias). Instituto de Veterin?ria, Departamento de Parasitologia Animal,
Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2012.
A utiliza??o de antibi?ticos no controle das infec??es intramam?rias e na elimina??o de
prov?veis fontes de infec??o nas fazendas leiteiras se constitui em importante medida de
controle. No entanto, o uso inadequado de antibi?ticos no tratamento da doen?a pode
gerar o aparecimento de cepas resistentes e comprometer a efici?ncia do tratamento.
Bact?rias do g?nero Staphylococcus spp. est?o entre os principais agentes etiol?gicos da
mastite bovina e s?o freq?entemente resistentes aos antimicrobianos, em especial aos
beta-lact?micos, principalmente por dois mecanismos distintos: a produ??o da enzima
extracelular beta-lactamase, codificada pelo gene blaZ, e a produ??o de PBP2a ou
PBP2?, uma prote?na ligante de penicilina de baixa afinidade, codificada pelo gene
mecA. A express?o do gene mecA ? constitutiva ou induzida por antibi?ticos
betalact?micos, como a oxacilina e cefoxitina. O gene mecA est? inserido no
cromossomo estafiloc?cico atrav?s de um elemento gen?tico m?vel, denominado
cassete estafiloc?cico cromoss?mico mec (SCCmec). O presente estudo avaliou o perfil
fenogenot?pico de resist?ncia aos beta-lact?micos em 250 isolados de Staphylococcus
spp, utilizando os marcadores oxacilina e cefoxitina, de modo a produzir dados que
possam contribuir para o conhecimento da resist?ncia antimicrobiana em algumas
propriedades leiteiras das regi?es Sul-Fluminense e Metropolitana do Estado do Rio de
Janeiro com o objetivo de subsidiar a implementa??o de medidas de controle dessa
enfermidade. A avalia??o da resist?ncia foi feita a partir de 8 diferentes testes
fenot?picos, sendo obtidos 54 perfis. Os testes de difus?o em disco simples e ?gar screen
com oxacilina foram utilizados como ?padr?o ouro? para os c?lculos dos valores de
sensibilidade, especificidade e predi??o por serem preconizados pelo CLSI veterin?rio.
O teste de difus?o em disco simples com cefoxitina foi o de melhor desempenho na
predi??o da resist?ncia a oxacilina. Na avalia??o genot?pica, n?o foi detectado qualquer
isolado positivo para o gene mecA, j? os genes mecI e mecRI foram detectados
igualmente em 11,6% (29/250) dos Staphylococcus spp avaliados. Foram detectados os
quatro tipos de cassete mec analisados (I, II, III e IV), sendo o tipo I o que teve mais
ampla distribui??o entre as regi?es estudadas. Gene blaZ foi detectado em 5,2%
(13/250) dos isolados, sendo que nestes, todo o sistema blaZ-blaI- blaR1 foi detectado
em 23,1% (3/13) dos isolados.
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Estrutura das comunidades de endoparasitos de seis esp?cies de peixes do Pantanal Norte Matogrossense, BrasilSANTOS, S?nia Maria Cursino dos 06 October 2010 (has links)
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Previous issue date: 2010-10-06 / The Pantanal provides great biotic diversity, having different characteristics in times of flood and
drought, hosting around 263 species of fish. The studies of the ecological host-parasite
relationship in fishes of the Cuiab? River, North Pantanal (MT) are scarce. This study aimed to
study quantitatively and qualitatively the endoparasite communities of migratory fish of
commercial importance of this location, check the fluctuation patterns of these indices and their
relation to the total length and the sex of host, and the cluster analysis of the similarity between
the components communities of endoparasites from different hosts. The samples were taken
during the years 2005 to 2009 on the River Cuiab? - Pantanal National Park (PARNA-Pantanal) at
17 ? 50 '48 "S 57 ? 24' 6" W in the state of Mato Grosso, Brazil, 376 specimens:
Pseudoplatystoma corruscans - 50, P. fasciatum - 50, Piaractus mesopotamicus - 85, Prochilodus
lineatus - 72; Zungaro zungaro-50 and Salminus brasiliensis - 87. Twenty-three species of
endoparasites were identified: five digenean, eight cestodes, seven nematodes, two
acanthocephalan and one pentastomid. We collected 10,418 specimens of endoparasites, of wich
6162 (59.15%) were collected from P. mesopotamicus, 1249 (11.99%) of P. fasciatum, 1173
(11.26%) of Z. zungaro, 1167 (11.2%) of P. corruscans. The pattern of aggregate distribution of
parasites was observed for all species. The fishes from the family Pimelodidae analyzed showed
the greatest diversity of species, although without the presence of any dominant species of
endoparasites. The relationship of body length between the host parasite prevalence were positive
for the parasitism of P. lineatus by N. (N.) curemai and by A. compactum, and of P. corruscans
by S. oxycephala. On the other hand, parasitism of P. corruscans by Porrocaecum sp. showed a
negative relationship between abundance and host growth. In Z. zungaro only the abundance of P.
rugosa and Porrocaecum sp. showed positive relationship with the length of the fish. The sex of
the host had influenced significantly the abundance of N. (N) curemai in P. lineatus, of S.
spectatus in P. mesopotamicus and the prevalence of S. oxycephala in P. fasciatum, suggesting
the existence of ecological and behavioral differences between male and female hosts. Cluster
analysis of similarity between the compositions of endoparasites of migratory fish has resulted in
two main groups well differentiated, with only 16.03% similarity between them. The first group
was formed by piscivorous fish and the second by omnivores and detritivores. Among pimelodids
the composition of their communities have 50% of similarity reaching to 87.5% of similarity
between P. corruscans and P. fasciatum. The S. brasiliensis (Characidae) showed qualitative
similarity of 39.68% compared to the internal group comprised of Pimelodidae. The other main
group was composed by the omnivorous P. mesopotamicus (Characidae) and the detritivore P.
lienatus (Prochilodontidae). Prochilodus lineatus showed 22.22% of similarity in the composition
among their endoparasites. As the quantitative similarity, only a core group was formed. The
similarity between the pimelodids was 50.35%, reaching to 67.29% between P. faciatum and P.
corruscans. Salminus brasiliensis shared 30.46% of similarity between the quantitative
pimelodids and 12.41% between P. mesopotamicus which, in turn, showed only 2.02% of
quantitative similarity with P. lineatus. Among the migratory fish of commercial interest in
Northern Pantanal of Mato Grosso, the similarity on the composition and structure of their
endoparasite communities was strongly influenced by the phylogenetic relatedness hosts, as well
as by the piscivorous feeding habits, coupled with the distribution of the fishes at the water?s
column / O bioma Pantanal apresenta uma enorme diversidade bi?tica, possuindo diferentes caracter?sticas
nas ?pocas de cheia e seca, albergando cerca de 263 esp?cies de peixes. Escassos s?o os estudos
da rela??o ecol?gica parasito-hospedeiro em peixes do rio Cuiab?, ?rea do Pantanal Norte (MT).
Este estudo teve como objetivos estudar quantitativamente e qualitativamente as comunidades de
endoparasitos de peixes migradores de import?ncia comercial desta localidade, verificar os
padr?es de flutua??o destes ?ndices e sua rela??o com o comprimento total e o sexo dos
hospedeiros e analisar o agrupamento dos ?ndices de similaridade entre as comunidades
componentes de endoparasitos em diferentes hospedeiros. Foram coletados durante os anos de
2005 a 2009 no rio Cuiab? ? Parque Nacional do Pantanal (PARNA-Pantanal) a 17? 50? 48? S e
57? 24? 6? W do estado de Mato Grosso, Brasil, 376 esp?cimes: Pseudoplatystoma corruscans ?
50, P. fasciatum ? 50, Piaractus mesopotamicus ? 85, Prochilodus lineatus ?72; Zungaro
zungaro? 50 e Salminus brasiliensis ? 87. Vinte e tr?s esp?cies de endoparasitos foram
identificadas: cinco digen?ticos, oito cest?ides, sete nemat?ides, dois acantoc?falos e um
pentastom?deo. Foram coletados 10.418 esp?cimes de endoparasitos, sendo que 6162 (59,15%)
foram coletados de P. mesopotamicus, 1249 (11,99%) de P. fasciatum, 1173 (11,26%) de Z.
zungaro, 1167 (11,2%) de P. corruscans. O t?pico padr?o de distribui??o agregada foi observado
para todas as esp?cies, onde os pimelodideos analisados foram os que apresentaram maior
diversidade de esp?cies, embora sem a presen?a de nenhuma esp?cie de endoparasito dominante.
As rela??es do comprimento do corpo do hospedeiro entre a preval?ncia e a abund?ncia
parasit?ria foram positivas para o parasitismo de P. lineatus por N. (N.) curemai e A. compactum,
e de P.corruscans por S. oxycephala. Por outro lado, o parasitismo de P. corruscans por
Porrocaecum sp. apresentou rela??o negativa entre a abund?ncia e o crescimento do hospedeiro.
Em Z. zungaro apenas a abund?ncia de P. rugosa e Porrocaecum sp. apresentaram rela??o
positiva com o comprimento total do peixe. O sexo dos hospedeiros influenciou
significativamente a abund?ncia de N. (N.) curemai em P. lineatus, de S. spectatus em P.
mesopotamicus e a preval?ncia parasit?ria de S. oxycephala em P. fasciatum, sugerindo a
exist?ncia de diferen?as ecol?gicas e comportamentais entre hospedeiros machos e f?meas. A
an?lise de agrupamento da similaridade entre as composi??es de endoparasitos dos peixes
migradores resultou em 2 grupos principais bem diferenciados, com apenas 16,03% de
similaridade entre eles. O primeiro grupo foi formado pelos peixes pisc?voros e o segundo, por
peixes on?voros e detrit?voros. Entre os pimelod?deos analisados, a composi??o de suas
comunidades endoparasit?rias apresentaram similaridade qualitativa de 50%, chegando a 87,5%
entre P. corruscans e P. fasciatum. O Characidae S. brasiliensis apresentou 39,68% de
similaridade qualitativa em rela??o ao grupo interno formado por Pimelodidae. No outro grupo
principal, o Characidae on?voro P. mesopotamicus e o Prochilodontidae detrit?voro P. lineatus
apresentaram 22,22% de similaridade na composi??o entre suas comunidades endoparasit?rias.
Quanto a similaridade quantitativa, apenas um grupo principal foi formado. A similaridade entre
os pimelod?deos foi de 50,35%, chegando a 67,29% entre P. faciatum e P. corruscans. Salminus
brasiliensis compartilhou 30,46% de similaridade quantitativa entre os pimelod?deos e 12,41%
com P. mesopotamicus, que, por sua vez, apresentou apenas 2,02% de similaridade quantitativa
com P. lineatus. Dentre os peixes migradores de interesse comercial do Pantanal Norte Mato-
Grossense, a similaridade na composi??o e estrutura de suas comunidades endoparasit?rias foi
fortemente influenciada pela proximidade filogen?tica dos hospedeiros, assim como pelo h?bito
alimentar exclusivamente pisc?voro, associado ? disposi??o dos peixes na coluna d??gua.
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