[pt] Este trabalho apresenta um projeto mecânico de um robô lagartixa pneumática, capaz de se locomover em superfícies inclinadas em relação ao solo, através apenas de atuadores lineares que utilizam o ar comprimido como fonte de energia. Como parte fundamental do projeto mecânico neste trabalho, um sistema de garra é desenvolvido gerando vácuo mecanicamente, para haver uma economia de consumo energético no robô em comparação com os acessórios comerciais geralmente utilizados para esta tarefa de fixação. Com o protótipo de conceito fabricado e montado, o mesmo é submetido a uma bateria de testes com o intuito de posteriormente aplicar os dados obtidos em uma rede neural artificial, visando o aprendizado computacional dos movimentos do robô e, assim, sua otimização de velocidade em determinada sequência de movimentação. Após o treinamento desta rede neural, o protótipo é submetido a novos experimentos para verificar a eficiência do treinamento realizado e qual o impacto real obtido no robô. Finalmente, com a utilização de um sistema de câmeras, os deslocamentos do robô em diversas situações distintas são rastreados, visando gerar gráficos comparativos e analisar a repetibilidade e confiabilidade do sistema. / [en] This work presents the mechanical design of a pneumatic gecko robot, capable of moving on inclined surfaces with respect to the ground, using only linear actuators with compressed air as a source of energy. As a fundamental part of the mechanical design in this work, a claw system is developed by generating vacuum mechanically, significantly reducing the energy consumption of the robot when compared to commercial accessories generally used for this clamping task. With the concept prototype manufactured and assembled, a series of tests are conducted to later apply the collected data in an artificial neural network. This network allows the computational learning of the robot movements, and thus its speed optimization for a certain defined gait. After training this neural network, the prototype is submitted to new experiments to verify the efficiency of the training performed and the real impact obtained on the robot. Furthermore, with the use of a camera system, the movements of the robot along several different situations are tracked, generating comparative graphs to analyze the repeatability and reliability of the system.

The Master Thesis deals with logistic module innovation of information system ERP. The principle of innovation is based on implementation of heuristic algorithms which solve Travel Salesman Problems (TSP). The software MATLAB is used for analysis and tests of these algorithms. The goal of Master Thesis is the comparison of selections algorithm, which are suitable for economic purposes (accuracy of solution, speed of calculation and memory demands).

Synaptic plasticity describes efficacy changes in synaptic transmission and ranges in duration from tens to hundreds of milliseconds (short-term), to hours and days (long-term). Short-term plasticity plays crucial roles in synaptic computation, information processing, learning, working and short-term memory as well as its dysfunction in psychiatric and neurodegenerative diseases. The main aim of my PhD thesis was to determine the molecular mechanisms of different forms of presynaptic plasticity. Short-term facilitation increases neurotransmitter release in response to a high-frequency pair (paired-pulse facilitation; PPF) or train (train facilitation; TF) of presynaptic stimuli. Synaptotagmin 7 (Syt7) has been shown to act as residual calcium (Ca$_{res}$) sensor for PPF and TF at various synapses. Syt7 also seems to be involved in recovery from depression, whereas its role in neurotransmission remains controversial. My aim was to express Syt7 in a synapse where it is not normally found and determine how it affects short-term synaptic plasticity. Immunohistochemistry indicated that Syt7 is not localized to cerebellar climbing fibers (CFs). Wild-type (WT) and Syt7 knockout (KO) recordings at CF to Purkinje cell (CF-PC) synapses established that at near-physiological external calcium (Ca$_{ext}$) levels both genotypes displayed similar recovery from paired-pulse depression. In low Ca$_{ext}$,WT CF-PC synapses showed robust PPF, which turned out to be independent of Syt7. All my experiments strongly suggested that WT CFs do not express native Syt7, but display low Ca$_{ext}$ CF-PC PPF and TF. Thus, channelrhodopsin-2 and Syt7 were bicistronically expressed via AAV9 virus in CFs. This ectopic Syt7 expression in CFs led to big increases in low-Ca$_{ext}$ CF-PC facilitation, more than doubling PPF and more than tripling TF. While overexpression of Syt7 might turn out to have an effect on the initial release probability (pr), the observed CF-PC facilitation increase still critically depended on presynaptic Syt7 expression. And when comparing only cells in a defined EPSC1 amplitude range, the Syt7-induced increase in low-Ca$_{ext}$ PPF could not be accounted for by changes in initial pr, suggesting a general role for Syt7 as calcium sensor for facilitation. Another form of short-term plasticity, post-tetanic potentiation (PTP), is believed to be mediated presynaptically by calcium-dependent protein kinase C (PKC) isoforms that phosphorylate Munc18-1 proteins. It is unknown how generally applicable this mechanism is throughout the brain and if other proteins might be able to modulate PTP. Combining genetic (PKCαβy triple knockout [TKO] and Munc18-1SA knock-in [Munc18 KI] mice, in which Munc18- 1 cannot get phosphorylated) with pharmacological tools (PKC inhibitor GF109203), helped us show that PTP at the cerebellar parallel fiber to Purkinje cell (PF-PC) synapse seems to depend on PKCs but seems mostly independent of Munc18-1 phosphorylation. In addition, compared to WT animals, genetic elimination of presynaptic active zone protein Liprin-α3 led to similar PF-PC PTP and paired-pulse ratios (PPRs). At the hippocampal CA3-CA1 synapse previous pharmacological studies suggested that PKC mediates PTP. A genetic approach helped to show that calcium dependent PKCs do not seem to be required for CA3-CA1 PTP. Pharmacologically inhibiting protein kinase A as well as genetically eliminating Syt7 also had no effect on CA3-CA1 PTP. In addition, Ca IM-AA mutant mice, in which Ca$_{v}$2.1 channels have a mutated IQ-like motif (IM) so that it cannot get bound by calcium sensor proteins any more, not only displayed regular PTP, but also normal PPF and TF at CA3-CA1 synapses. In conclusion, my PhD thesis helped further characterize different forms of presynaptic plasticity, underlined that short-term synaptic plasticity can be achieved through diverse mechanisms across the Mammalian brain and supported a potentially general role for synaptotagmin 7 acting as residual calcium sensor for facilitation.

This diploma thesis deals with evolutionary algorithms used for travelling salesman problem (TSP). In the first section, there are theoretical foundations of a graph theory and computational complexity theory. Next section contains a description of chosen optimization algorithms. The aim of the diploma thesis is to implement an application that solve TSP using evolutionary algorithms.

The aim of this master thesis is proposal of a sport center building. The proposal consists of design of a detached building with room for climbing gym, sauna, massage room and also space for fitness, spinning and yoga. A bar with area for relaxing is a part of the building as well. The sport center has two floors and no basement. Due to the climbing gym, the second floor does not cover all disposition of the second floor. The support system of the building is skeletal. Part of the building´s roof is supported by steel truss frames with purlins, another part with solid panel trusses with purlins. The cieling is made of holoribs and reinforced concrete. The building is covered by single layer roof and has ventilated facade.

The diploma thesis is focused on a reconstruction of a cinema building in the town Gbely. It’s complete reconstruction of exterior and interior too. Original function, cinema, is change to sport and cultural centrum now. Object is consist of cafe, squash hall, climbing wall and sauna with rest room. Several thermal insulations are used to isolate object and satisfy a norm terms. The foundations are post insulated too. The interior is divided into functional spaces with Heluz system. New finishes in the object is created by brick paving, klinker, ceramic tiles and tiling. All windows and doors are replaced by new ones in the object. Sliding doors are used in interior for save space. The windows and doors are plastic between interior and exterior. All sanitations are replaced by new ones too. The reinforced concrete floor is used instead of lead bearing lintels. The wheelchair conditions are satisfied on ground floor.

Background: Knee osteoarthritis is a degenerative disease characterized by damaged joint tissues (e.g., cartilage) that leads to joint pain, and reduced mobility and quality of life. Various factors are involved in disease progression, including biomechanical, patient-reported outcome and mobility measures. This thesis provides important longitudinal data on the role of these factors in disease progression, and the trajectory of biomechanical factors in persons with knee osteoarthritis. Objectives: (1) Determine the extent to which changes over 2.5 years in knee cartilage thickness and volume in persons with knee osteoarthritis were predicted by the knee adduction and flexion moment peaks, and knee adduction moment impulse and loading frequency. (2) Determine the extent to which changes over 2 years in walking and stair-climbing mobility in women with knee osteoarthritis were predicted by quadriceps strength and power, pain and self-efficacy. (3) Estimate the relative and absolute test-retest reliabilities of biomechanical risk factors for knee osteoarthritis progression. Methods: Data were collected at 3-month intervals during a longitudinal (3-year), observational study of persons with clinical knee osteoarthritis (n=64). Magnetic resonance imaging of the study knee was acquired at the first and last assessments, and used to determine cartilage thickness and volume. Accelerometry and dynamometry data were acquired every 3 months, and used to determine knee loading frequency and knee muscle strength and power, respectively. Walking and stair-climbing mobility, as well as pain and self-efficacy data, were also collected every 3 months. Gait analyses were performed every 6 months, and used to calculate lower-extremity kinematics and kinetics. Results: (1) The knee adduction moment peak and impulse each interacted with body mass index to predict loss of medial tibial cartilage volume over 2.5 years. These interactions suggested that larger joint loads in those with a higher body mass index were associated with greater loss of cartilage volume. (2) In women, lower baseline self-efficacy predicted decreased walking and stair ascent performances over 2 years. Higher baseline pain intensity/frequency also predicted decreased walking performance. Quadriceps strength and power each interacted with self-efficacy to predict worsening stair ascent times. These interactions suggested that the impact of lesser quadriceps strength and power on worsening stair ascent performance was more important among women with lower self-efficacy. (3) Relative reliabilities were high for the knee adduction moment peak and impulse, quadriceps strength and power, and body mass index (i.e., intraclass correlation coefficients >0.80). Absolute reliabilities were high for quadriceps strength and body mass index (standard errors of measurement <15% of the mean). Data supported the use of interventions effective in reducing the knee adduction moment and body mass index, and increasing quadriceps strength, in persons with knee osteoarthritis. Conclusion: Findings from this thesis suggest that biomechanical factors play a modest independent role in the progression of knee osteoarthritis. However, in the presence of other circumstances (e.g., obesity, low self-efficacy, high pain intensity/frequency), biomechanical factors can vastly worsen the disease. Strategies aiming to curb structural progression and improve clinical outcomes in knee osteoarthritis should target biomechanical and clinical outcomes simultaneously. / Thesis / Doctor of Philosophy (PhD) / Knee osteoarthritis is a multifactorial disease whose progression involves worsening joint structure, symptoms, and mobility. Various factors are linked to the progression of this disease, including biomechanical, patient-reported outcome and mobility measures. This thesis provides important information on how these factors, separately and collectively, are involved in worsening disease over time, as well as benchmarks that are useful to clinicians and researchers in interpreting results from interventional or longitudinal research. First, we examined how different elements of knee loading were associated with changes in knee cartilage quantity over time in persons with knee osteoarthritis. Second, we examined how different elements of knee muscle capacity and patient-reported outcomes were related to changes in mobility over time in persons with knee osteoarthritis. Third, we examined the stability over time of various biomechanical risk factors for the progression of knee osteoarthritis. Novel results from this thesis showed that: (1) larger knee loads predicted cartilage loss over 2.5 years in obese individuals with knee osteoarthritis but not in persons of normal weight or overweight; (2) among women with knee osteoarthritis with lower self-efficacy (or confidence), lesser knee muscle capacity (strength, power) was an important predictor of declining stair-climbing performance over 2 years; and (3) clinical interventions that can positively alter knee biomechanics include weight loss, knee muscle strengthening, as well as specific knee surgery and alterations during walking to reduce knee loads. Interventions for knee osteoarthritis should target biomechanical and clinical outcomes simultaneously.

Prior to this thesis, there was a specialist course “Sport centre Za Lužánkami Brno”. This project was focused on planning of urbanism-architectural area with a mixture of renovated and new facilities with different functions for sport and leisure, together with professional sport opportunities. The emphasis was on collision-free paths for pedestrians and cyclists, clear transport links around the centre and terrain configuration of the area. The thesis follows on from this project, focusing on development between the hotel Bobycentrum in the South and the Lužánky swimming pool in the North. The idea of the proposal stems from the need to connect the development area to the existing network of pedestrian and cycle paths and the public transport system to minimise the use for individual car travel to the sport centre; and to connect existing and new facilities for leisure and sports. The Sport relax centre was designed with existing buildings in mind and partially follows their ground floor dimensions. The centre creates a barrier from the noisy and busy street and provides a calmer area for outdoor recreation. It fills a gap between the existing buildings and is encircled by a footpath at second level joining to the footbridges over Sportovní Street and the existing terrain in the north-western part. The footpath is ended by passageway between swimming pool and wellness centre. More and more people are looking for sport opportunities in cities however; there are still not enough quality grounds and facilities on offer. The facilities in this development are meant for regular trainings and recreation for the public, including parents with children, groups of friends as well as individual and team sports players, offering a range of day, evening and night activities both indoors and outdoors. Other facilities include outdoor changing rooms and sports equipment rental as well as spaces for meeting friends such as a restaurant, wellness and a dancing-hall.

The subject of the diploma thesis is to design a project study and a building program that would become a compromise between the requirements of the city of Brno and BUT in the area of Kraví hora in the Brno city center. The city of Brno would like to transform the area into a public park. But BUT considers it as its "family silver" because they realize its extraordinary value. Options of a constructions are really limited because of many area regulations. My proposal counts on the demolition of all existing buildings in the area, respects property area relations , protection zone, continuity with existing traffic roads, height regulation, imaginary raster of surrounding buildings, slope of terrain and orientation to the cardinal points. My suggest represents a study of five groups of objects which creating a rectangular raster. As a compromise solution, I chose the presentation and promotional areas of the individual faculties of BUT for the content of the buildings. Faculties have spaces for hobby groups for the public comprise all age groups included. I also designed the BUT Museum of History, a multimedia hall, a cafeteria, studios, a student leisure center and an observation tower with a climbing wall.

The assignment is an architecture and urban planning study of a sports and recreation center. The solved area is located in the town of Vsetín, in the part called Ohrada. Nowdays, the area is obsolete, unfunctional and also unmodern. My project is mainly focused to create a concept that responds to the environment, offers outdoor and indoor spaces for all generations. It allows better connection with nature, variable use, and division of area by function. The design is defined by the river Bečva on the west side and a busy 1st class road on the east side. It was important to separate pedestrians and cyclists from motor vehicles. The response to this restriction is to direct traffic in the area to the west, towards the river. This creates a terrain works that optically deflects the design in a quieter direction. Buildings are placed in the line and oriented in the western direction. After creating the terrain, I divided the area into several segments according to functionality. In the heart of the area, a multifunctional central hall with an inner climbing wall is set in the terrain. In front of it is a public space for meetings, sports and recreation. On the other side, there is a basketball court, a workout playground, a children's playground in front of the hall and a rest area of ??greenery closer to the river. If we continue southerly down, another cube set into the terrain is a building with a restaurant and a cafe. The southernmost building is a tennis club with facilities for athletes and squash. In front of the building there is a 9x tennis court with a grandstand. Closer to the river and the bike path there is an outdoor climbing wall. From the newly created embankment, the area is divided by a cycle path around the river. Behind the bridge is a green belt separated area which, unlike the previous ones, is tuned in a calmer spirit. We will find a building of a yoga and meditation center.