Tuatara (Sphenodon spp.) were once widespread over both the North and the South Islands of New Zealand approximately 1000 years ago but are now restricted to offshore islands due to introduced predators and habitat destruction. The survival of tuatara is now threatened by climate change because of isolation on islands that prevent them from migrating to cooler regions, and by their limited capacity to adapt due to their long life span and low genetic diversity. The thermal suitability of cooler regions for future translocations could be the key for tuatara conservation. The overall aim of the study was to determine the effect of cool temperature on the physiology, behaviour and egg incubation of Cook Strait tuatara (Sphenodon punctatus). As well answering specific questions about tuatara and their adaptation to cool temperature this study aimed at answering general questions about the behavioural and evolutionary ecology of reptiles. I first compared the preferred body temperature, feeding responses and tolerance to cold temperature of tuatara (Sphenodon punctatus) with three lizard species that inhabit the Otago region of southern New Zealand: Hoplodactylus maculatus, Naultinus gemmeus and Oligosoma maccanni. As well as testing the co-adaptation hypothesis between preferred body temperature and physiological performance of reptiles, I wanted to determine whether tuatara (which are planned to be reintroduced from a warmer site to the Otago region) have similar responses to cool temperature as do Otago lizard species. I found that tuatara show responses to cold temperatures similar to those of lizards from southern New Zealand, suggesting that if tuatara are translocated outside of their geographical range, they are likely to survive. I then tested if tuatara were capable of modifying their thermoregulatory behaviour if translocated to a cooler region. To do so, I conducted a laboratory experiment in which tuatara were provided with three thermal treatments corresponding to the thermal environments they would experience in their current habitat and the proposed reintroduction site. Contrary to the prediction of the cost-benefit model of thermoregulation, tuatara became active thermoregulators when the thermal quality of the habitat decreased. The results suggest that the model is less applicable to cold-adapted species and that if translocated to cooler regions, tuatara will be able to adjust their thermoregulatory behaviour to their thermal environment. Tuatara are temperature-dependent sex determination (TSD) reptiles and their translocation to cooler regions could have an dramatic impact on the sex ratio of new populations. To determine if potentially lower incubation temperature would be a limiting factor for the reintroduction of tuatara, I translocated eggs to the proposed reintroduction site. Some tuatara embryos developed at the site (and later hatched in the laboratory), but incubation lasted longer than in natural nests and all hatchlings were female. This trend observed in biased sex ratio might change in the context of climate change as an increase of soil temperature by 3�C would allow the production of males at the proposed site. The present study demonstrates that the translocation of tuatara further south, outside of their current geographical range, is possible and recommended. Tuatara show similar responses to cool temperatures compared with lizards that live in southern New Zealand, they can also adjust their thermoregulatory behaviour if translocated to cooler habitats, and tuatara embryos could potentially develop successfully in cooler regions. The greatest chance for the future survival of tuatara and TSD species in general, lies in translocation to other locations that are thermally suitable.

To identify a model legume experimental system for studying low temperature signaling and cold acclimation, cold-induced expression and regulation of homologues of alfalfa (Medicago sativa) cold acclimation-specific genes cas15 and cas30 were examined in M. arborea (relatively frost tolerant) and M. truncatula (relatively frost sensitive). Both cas15 and cas30 genes are present in the genomes of both species but whereas both genes are cold-induced in M. arborea, only cas15 is induced in M. truncatula. Cold-induced expression of these genes is inhibited by calcium chelators and channel blockers and by the membrane fluidizer benzyl alcohol. Treatment of leaves with dimethylsulfoxide, a membrane rigidifier, induced both genes at 25°C. A cold-activated MAP kinase activity was expressed in both species. These results suggest that M. truncatula, an annual, self-pollinated species may be successfully used as model experimental systems in studies of cold signaling and role of cas genes in cold acclimation in legumes.

Tuatara (Sphenodon spp.) were once widespread over both the North and the South Islands of New Zealand approximately 1000 years ago but are now restricted to offshore islands due to introduced predators and habitat destruction. The survival of tuatara is now threatened by climate change because of isolation on islands that prevent them from migrating to cooler regions, and by their limited capacity to adapt due to their long life span and low genetic diversity. The thermal suitability of cooler regions for future translocations could be the key for tuatara conservation. The overall aim of the study was to determine the effect of cool temperature on the physiology, behaviour and egg incubation of Cook Strait tuatara (Sphenodon punctatus). As well answering specific questions about tuatara and their adaptation to cool temperature this study aimed at answering general questions about the behavioural and evolutionary ecology of reptiles. I first compared the preferred body temperature, feeding responses and tolerance to cold temperature of tuatara (Sphenodon punctatus) with three lizard species that inhabit the Otago region of southern New Zealand: Hoplodactylus maculatus, Naultinus gemmeus and Oligosoma maccanni. As well as testing the co-adaptation hypothesis between preferred body temperature and physiological performance of reptiles, I wanted to determine whether tuatara (which are planned to be reintroduced from a warmer site to the Otago region) have similar responses to cool temperature as do Otago lizard species. I found that tuatara show responses to cold temperatures similar to those of lizards from southern New Zealand, suggesting that if tuatara are translocated outside of their geographical range, they are likely to survive. I then tested if tuatara were capable of modifying their thermoregulatory behaviour if translocated to a cooler region. To do so, I conducted a laboratory experiment in which tuatara were provided with three thermal treatments corresponding to the thermal environments they would experience in their current habitat and the proposed reintroduction site. Contrary to the prediction of the cost-benefit model of thermoregulation, tuatara became active thermoregulators when the thermal quality of the habitat decreased. The results suggest that the model is less applicable to cold-adapted species and that if translocated to cooler regions, tuatara will be able to adjust their thermoregulatory behaviour to their thermal environment. Tuatara are temperature-dependent sex determination (TSD) reptiles and their translocation to cooler regions could have an dramatic impact on the sex ratio of new populations. To determine if potentially lower incubation temperature would be a limiting factor for the reintroduction of tuatara, I translocated eggs to the proposed reintroduction site. Some tuatara embryos developed at the site (and later hatched in the laboratory), but incubation lasted longer than in natural nests and all hatchlings were female. This trend observed in biased sex ratio might change in the context of climate change as an increase of soil temperature by 3�C would allow the production of males at the proposed site. The present study demonstrates that the translocation of tuatara further south, outside of their current geographical range, is possible and recommended. Tuatara show similar responses to cool temperatures compared with lizards that live in southern New Zealand, they can also adjust their thermoregulatory behaviour if translocated to cooler habitats, and tuatara embryos could potentially develop successfully in cooler regions. The greatest chance for the future survival of tuatara and TSD species in general, lies in translocation to other locations that are thermally suitable.

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Snow algae as typical extremophiles are good model organisms for study of adaptation for life on the boundary of physiological possibilities. So far, it is not clear, how these microorganisms ensure on the molecular level the optimization of photosynthetic processes in conditions around 0 řC, often with very high light intensity. The aim of this work was to find out light and temperature growth optima of two less studied strains and to assess the composition of fatty acids in selected psychrophilic and psychrotrophic strains from the genera Chloromonas and Chlamydomonas (Chlamydomonadales, Chlorophyta) from culture collections UTEX and CCCryo and collections in Europe including the Czech Republic and Slovakia. Using crossed gradients method, this thesis describes optimal temperature and light conditions of two strains of snow algae isolated from sites in the Krkonoše Mountains that are characterized by different ecological conditions. The strain Chloromonas reticulata Luční originates from alpine zone and according to its growth characteristics, it can be classified as psychrotrophic alga requiring high light. The second tested strain was Chloromonas pichinchae Meandry from forest environment, which is also characterized as psychrotrophic, In contrast to previous strain, it grows in a wide range of...

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