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GENOMIC STUDIES OF NOVEL BEHAVIOR TRAITS IN LACTATING SOWS AND THEIR RELATIONSHIP TO HEAT STRESS RESILIENCE AND MATERNAL PERFORMANCESharlene Olivette Hartman (18523692) 09 May 2024 (has links)
<p dir="ltr">Heat stress is one of the leading welfare concerns for modern swine production globally. Swine are especially susceptible as the species has inactive sudoriferous glands and, as a result, these animals thermoregulate mainly through behaviors such as wallowing and rooting up cooler patches of ground. In commercial settings, access to open ground or wallowing pits would present a threat to the animals’ health in the form of a vector for diseases and parasites. This puts aspects of swine welfare in direct conflict with one another, while public legislature and consumer pressures increasingly call for approaches to animal welfare that encompass all aspects of welfare to be considered. Efforts towards improved welfare are also crucial to the environmental and economical sustainability for producers and consumers. Previous genetic selection tactics used in swine herds focused intensely on a few traits and have led to modern animals being highly productive but also more environmentally sensitive. Intensely selected animals have energy margins that are narrower and more devoted to higher production leaving less accommodations for resilience in moments of stress. Since swine behaviorally thermoregulate, the use of behavior studies combined with those of genetics is one possible way to effectively identify heritable aspects of climatic resilience into future herds while still improving production traits. The primary objectives of this thesis were to develop an ethogram that can be used to base phenotypes of behavior; calculate the variance components of the behavior traits; correlate these components with those of maternal productivity and heat tolerance; and finally explore the genetic background of the behavior traits based on the identification of genomic markers significantly associated with them. Data was collected on 1,678 Landrace × Large White sows from a commercial swine herd in North Carolina. The data comprised information on climatic factors, litter performance, and behavior during a standardized human handling procedure in the summer of 2021. These animals were genotyped using the PorcineSNP50K Bead Chip (Illumina, San Diego, CA, USA). These studies describe the development of an ethogram for use on lactating sows in a commercial setting in order to create the novel behavior traits of Responsiveness Score (RS), Vocalization Score (VS), and Shave Time (ST). The genetic variance components were then estimated, and heritabilities found to be 0.17 ± 0.05 for RS, 0.15 ± 0.05 for VS, and 0.10 ± 0.05. These novel behavior traits had low genetic correlations with traits of maternal performance and heat stress resilience. Genome-wide association studies were then performed, and 19 genomic markers were significantly associated with the three behavior traits. However, only one region located on chromosome 3 harbors a candidate gene that may play a role in heat stress resilience. Although the novel behavior traits explored in this study are heritable, they were not found to be viable indicator traits for heat stress resilience due to their low genetic correlations with direct indicators of heat tolerance in lactating sows. It is important that additional phenotypic records of behaviors related to welfare are generated in the future to better map their polygenic nature. </p>
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Vocal communication in bonobos (Pan paniscus) : studies in the contexts of feeding and sexClay, Zanna January 2011 (has links)
Despite having being discovered nearly 80 years ago, bonobos (Pan paniscus) are still one of the least well understood of the great apes, largely remaining in the shadow of their better known cousins, the chimpanzees (Pan troglodytes). This is especially evident in the domain of communication, with bonobo vocal behaviour still a neglected field of study, especially compared to that of chimpanzees. In this thesis, I address this issue by exploring the natural vocal communication of bonobos and its underlying cognition, focusing on the role that vocalisations play during two key contexts, food discovery and sex. In the context of food-discovery, I combine observational and experimental techniques to examine whether bonobos produce and understand vocalisations that convey meaningful information about the quality of food encountered by the caller. Results indicate that bonobos produce an array of vocalisations when finding food, and combine different food-associated calls together into sequences in a way that relates to perceived food quality. In a subsequent playback study, it was demonstrated that receivers are able to extract meaning about perceived food quality by attending to these calls and integrating information across call sequences. In the context of sexual interactions, I examine the acoustic structure of female copulation calls, as well as patterns in call usage, to explore how these signals are used by individuals. My results show that females emit copulation calls in similar ways with both male and female partners, suggesting that these signals have become partly divorced from a function in reproduction, to assume a greater social role. Overall, my results highlight the relevance of studying primate vocalisations to investigate the underlying cognition and suggest that vocalisations are important behavioural tools for bonobos to navigate their social and physical worlds.
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Os Blues Poems de Langston Hughes: por uma tradução musicada / Langston Hughess Blues Poems: a musical translationOliveira, Pedro Tomé de Castro 02 June 2017 (has links)
Se a tradução envolve leitura e reescrita, nós poderíamos questionar o modo como se lê antes de questionar o modo como se reescreve. A tradução de poesia parece continuamente lidar com problemas de ritmo, rima, sintaxe, sentido, registro linguístico. Mas e se o tradutor, entendendo a leitura como performance, recolocar tais questões sob a perspectiva de outra mídia, que possa libertá-lo das restrições da escrita? O pesquisador Peter Low (2003) provoca os tradutores de poesia a se questionarem se desejam que seus textos sejam vocalizados (recitados, musicados etc.), e não apenas lidos silenciosamente. Haveria, então, uma alteração funcional e, consequentemente, um texto de chegada com um skopos diferente (REISS e VERMEER, 1996). Trata-se de trabalhar com a força latente da voz no texto; com uma possibilidade de performance inscrita na mídia escrita: a vocalidade, como coloca Paul Zumthor (1993). O poeta negro estadunidense Langston Hughes (1902-67) escrevia poemas semelhantes a letras de blues e recitava seus versos no ato da criação, o que pode sugerir algo a respeito de um possível modo de ler, absorver e recriar sua poesia em outra língua. Nosso objetivo, portanto, é traduzir seus poemas de blues ao cantá-los e tocá-los no violão. As canções de blues resultantes, em português, estão registradas em CD que foi inserido como apêndice da tese. Nosso método consiste em musicá-los enquanto os traduzimos, pois a simultaneidade dos processos é precisamente aquilo que interfere nas escolhas linguísticas, influenciando aspectos de ritmo, sintaxe, sentido etc. Nossa hipótese envolve, então, a questão de como os modos de dizer da canção popular, numa dada língua-cultura, determinariam o resultado da tradução. Essa experimentação deve, segundo esperamos, contribuir para os Estudos da Tradução por redimensionar alguns aspectos do texto de acordo com a dinâmica da vocalização. Esses resultados são tanto acadêmicos, em termos da discussão sobre a própria tradução, quanto artísticos, como música de blues composta para o público brasileiro, apresentando Langston Hughes, relativamente pouco conhecido e traduzido no Brasil, na forma de um gênero musical que é amplamente disseminado por aqui. / If translation involves reading and rewriting, we might question how we read a text before questioning the rewriting. Poetry translation seems to continually deal with problems of rhythm, rhyme, syntax, meaning, register. What if the translator understands reading as performance in order to reconceive all those issues within the perspective of another medium, which could free him from the constraints of writing? Researcher Peter Low (2003) suggests that poetry translators ask themselves whether they would like their texts to be recited, set to music etc., rather than just silently read. There would be, then, a functional alteration, and the target text would have a different skopos (REISS and VERMEER, 1996). We propose working with the latent force of the voice in the text; with a possibility of performance inscribed in the written medium: the vocality of Paul Zumthor (1993). The Afro-American poet Langston Hughes (1902-67) wrote poems very similar to blues lyrics and knowingly spoke (or even sung) his lines while creating them. That might suggest something about a way to read, absorb and recreate his poetry in another language. Our goal, therefore, is to translate his blues poems while singing and playing them on the guitar. The resulting blues songs in Portuguese are registered on a CD attached to this thesis. Our method is to set them to music while translating them, because the simultaneity of the processes is exactly what might interfere in the linguistic choices, influencing aspects of rhythm, meaning etc. Our hypothesis involves, then, the discussion of how the modes of expression of the popular song, in a given system of language-culture, would determine the results of the translation. That sort of experimentation will hopefully contribute to the Translation Studies by rearranging certain aspects of the text according to the dynamics of vocalization. These results are both academic, in terms of the discussion about translation itself; and artistic, as blues music composed for the Brazilian audience, presenting Langston Hughes, relatively unknown and non-translated in Brazil, in the form of a musical genre which is widely disseminated here.
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Sound use, sequential behavior and ecology of foraging bottlenose dolphins, Tursiops truncatusNowacek, Douglas Paul January 1999 (has links)
Thesis (Ph. D.)--Joint Program in Biological Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 1999. / Includes bibliographical references. / Odontocetes are assumed to use echolocation for navigation and foraging, but neither of these uses of biosonar has been conclusively demonstrated in free-ranging animals. Many bats are known to use echolocation throughout foraging sequences, changing the structure and timing of clicks as they progress towards prey capture. For odontocetes, however, we do not know enough about their foraging behavior to describe such sequences. To conduct detailed behavioral observations of any subject animal, the observer must be able to maintain continuous visual contact with the subject for a period commensurate with the duration of the behavior(s) of interest. Behavioral studies of cetaceans, which spend approximately 95% of their time below the water's surface, have been limited to sampling surface behavior except in special circumstances, e.g. clear-water environments, or with the use of technological tools. I addressed this limitation through development of an observation platform consisting of a remote controlled video camera suspended from a tethered airship with boat-based monitoring, adjustment, and recording of video. The system was used successfully to conduct continuous behavioral observations of bottlenose dolphins in the Sarasota Bay, FL area. This system allowed me to describe previously unreported foraging behaviors and elucidate functions for behaviors already defined but poorly understood. Dolphin foraging was modeled as a stage-structured sequence of behaviors, with the goal-directed feeding event occurring at the end of a series of search, encounter, and pursuit behaviors. The behaviors preceding a feeding event do not occur in a deterministic sequence, but are adaptive and plastic. A single-step transition analysis beginning with prey capture and receding in time has identified significant links between observed behaviors and demonstrated the stage-structured nature of dolphin foraging. Factors affecting the occurrence of specific behaviors and behavioral transitions include mesoscale habitat variation and individual preferences. The role of sound in foraging, especially echolocation, is less well understood than the behavioral component. Recent studies have explored the use of echolocation in captive odontocete foraging and presumed feeding in wild animals, but simultaneous, detailed behavioral and acoustic observations have eluded researchers. The current study used two methods to obtain acoustic data. The overhead video system includes two towed hydrophones used to record 'ambient' sounds of dolphin foraging. The recordings are of the 'ambient' sounds because the source of the sounds, i.e. animal, could not be localized. Many focal follows, however, were conducted with single animals, and from these records the timing of echolocation and other sounds relative to the foraging sequence could be examined. The 'ambient' recordings revealed that single animals are much more vocal than animals in groups, both overall and during foraging. When not foraging, single animals vocalized at a rate similar to the per animal rate in groups of>=2 animals. For single foraging animals, the use of different sound types varies significantly by the habitat in which the animal is foraging. These patterns of use coupled with the characteristics of the different sound types suggest specific functions for each. The presence of multiple animals in a foraging group apparently reduces the need to vocalize, and potential reasons for this pattern are discussed. In addition, the increased vocal activity of single foraging animals lends support to specific hypotheses of sound use in bottlenose dolphins and odontocetes in general. The second acoustic data collection method records sounds known to be from a specific animal. An acoustic recording tag was developed that records all sounds produced by an animal including every echolocation click. The tag also includes an acoustic sampling interval controller and a sensor suite that measures pitch, roll, heading, and surfacing events. While no foraging events occurred while an animal was wearing an acoustic data logger, the rates of echolocation and whistling during different activities, e.g. traveling, were measured. / by Douglas Paul Nowacek. / Ph.D.
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Participação dos receptores NK-1 dos núcleos basolateral e central da amígdala no comportamento defensivo de ratos / Involvement of NK-1 receptors of the basolateral and central nuclei of the amygdala in the defensive behavior of ratsBassi, Gabriel Shimizu 29 June 2012 (has links)
Estudos realizados na última década mostram que a substância P (SP) é um neuromediador importante de estados emocionais e afetivos. A SP tem ação pró-aversiva quando microinjetada na substância cinzenta periaquedutal dorsal (SCPd) através da ativação de receptores neurocininérgicos do tipo NK-1, uma vez que o comportamento defensivo é bloqueado por antagonistas desses receptores. A ativação de receptores NK-1 na SCPd também produz antinocicepção, a qual é considerada parte da reação de defesa. Na sequência desses estudos, este projeto visa investigar o envolvimento dos receptores neurocininérgicos no núcleo central (CeA) e basolateral (BLA) da amígdala na mediação dos estados aversivos gerados e elaborados nessa estrutura prosencefálica que, junto com a SCPd, faz parte do sistema encefálico aversivo. O presente estudo mostrou que a SP e o agonista NK-1 (Sar-Met-SP) promoveram efeitos pró-aversivos no labirinto em cruz elevado somente no CeA, mas não no BLA. Ao contrário da SCPd, não obtivemos qualquer alteração no limiar nociceptivo com a microinjeção de antagonista de receptores NK-1 (Spantide) em ambos os núcleos. O Spantide sozinho não alterou os indicadores de nocicepção e ansiedade. Nenhum tipo de vocalização (audível ou ultrassônica) foi detectado no presente trabalho após a microinjeção de SP ou Sar-Met-SP em ambos núcleos amigdalóides, apesar de relatos de vocalizações ultrassônicas (VUS) após o mesmo tipo de tratamento na SCPd. Os resultados obtidos no presente estudo mostraram que o CeA, mas não o BLA, modula a expressão de comportamentos relacionados ao medo inato através de receptores neurocininérgicos do tipo NK-1. VUS e antinocicepção não parecem participar da reação de defesa elaborada no CeA. A ausência da emissão de VUS nesses núcleos pode indicar que somente estruturas mais antigas do neuroeixo (mesencéfalo e hipotálamo) são responsáveis pela produção de VUS. / A substantial body of evidence obtained in the last decade demonstrated that the Substance P (SP) is an important mediator of the affective and emotional behaviors. SP is a pro-aversive compound when microinjected within the dorsal periaqueductal gray (dPAG). These effects are mediated by the type 1 neurokininergic receptors (NK-1), since the defensive behavior was inhibited by antagonists of these receptors. The activation of NK-1 receptors in the dPAG also produced antinociception and ultrasonic vocalizations (USV). In the sequence of these studies, this study investigated the involvement of neurokinin receptors of the central (CeA) and basolateral (BLA) nuclei of the amygdala in the mediation of the defense reaction. The amigdala together with the dPAG and the medial hypothalamus comprise the encephalic aversive system. The results showed that SP and the NK-1 agonist (Sar-Met-SP) promoted pro-aversive effects in the elevated plus maze test only when microinjected into the CeA, without effect in the BLA. Although SP and the activation of NK-1 receptors induce antinociception in the dPAG, we did not observe any alteration of the nociceptive threshold in the tail-flick test with the NK-1 antagonist (Spantide) injected into both nuclei and any changes of the anxiety parameters in the EPM. No vocalizations (audible or ultrasonic) were detected after treatment with SP or Sar-Met-SP in both amygdaloid nuclei. The lack of emissions of USVs after activation of these nuclei could indicate that only older structures (PAG and hypothalamus) of the neuroaxis are responsible for the production of USVs. The results obtained in the present study show that NK-1 receptors within CeA, but not BLA, modulate the expression of defensive behaviors related to the innate fear. Apparentely USVs and antinociception are not involved in the defensive reactions indiced by activation of NK-1 mechanisms in the CeA.
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Os Blues Poems de Langston Hughes: por uma tradução musicada / Langston Hughess Blues Poems: a musical translationPedro Tomé de Castro Oliveira 02 June 2017 (has links)
Se a tradução envolve leitura e reescrita, nós poderíamos questionar o modo como se lê antes de questionar o modo como se reescreve. A tradução de poesia parece continuamente lidar com problemas de ritmo, rima, sintaxe, sentido, registro linguístico. Mas e se o tradutor, entendendo a leitura como performance, recolocar tais questões sob a perspectiva de outra mídia, que possa libertá-lo das restrições da escrita? O pesquisador Peter Low (2003) provoca os tradutores de poesia a se questionarem se desejam que seus textos sejam vocalizados (recitados, musicados etc.), e não apenas lidos silenciosamente. Haveria, então, uma alteração funcional e, consequentemente, um texto de chegada com um skopos diferente (REISS e VERMEER, 1996). Trata-se de trabalhar com a força latente da voz no texto; com uma possibilidade de performance inscrita na mídia escrita: a vocalidade, como coloca Paul Zumthor (1993). O poeta negro estadunidense Langston Hughes (1902-67) escrevia poemas semelhantes a letras de blues e recitava seus versos no ato da criação, o que pode sugerir algo a respeito de um possível modo de ler, absorver e recriar sua poesia em outra língua. Nosso objetivo, portanto, é traduzir seus poemas de blues ao cantá-los e tocá-los no violão. As canções de blues resultantes, em português, estão registradas em CD que foi inserido como apêndice da tese. Nosso método consiste em musicá-los enquanto os traduzimos, pois a simultaneidade dos processos é precisamente aquilo que interfere nas escolhas linguísticas, influenciando aspectos de ritmo, sintaxe, sentido etc. Nossa hipótese envolve, então, a questão de como os modos de dizer da canção popular, numa dada língua-cultura, determinariam o resultado da tradução. Essa experimentação deve, segundo esperamos, contribuir para os Estudos da Tradução por redimensionar alguns aspectos do texto de acordo com a dinâmica da vocalização. Esses resultados são tanto acadêmicos, em termos da discussão sobre a própria tradução, quanto artísticos, como música de blues composta para o público brasileiro, apresentando Langston Hughes, relativamente pouco conhecido e traduzido no Brasil, na forma de um gênero musical que é amplamente disseminado por aqui. / If translation involves reading and rewriting, we might question how we read a text before questioning the rewriting. Poetry translation seems to continually deal with problems of rhythm, rhyme, syntax, meaning, register. What if the translator understands reading as performance in order to reconceive all those issues within the perspective of another medium, which could free him from the constraints of writing? Researcher Peter Low (2003) suggests that poetry translators ask themselves whether they would like their texts to be recited, set to music etc., rather than just silently read. There would be, then, a functional alteration, and the target text would have a different skopos (REISS and VERMEER, 1996). We propose working with the latent force of the voice in the text; with a possibility of performance inscribed in the written medium: the vocality of Paul Zumthor (1993). The Afro-American poet Langston Hughes (1902-67) wrote poems very similar to blues lyrics and knowingly spoke (or even sung) his lines while creating them. That might suggest something about a way to read, absorb and recreate his poetry in another language. Our goal, therefore, is to translate his blues poems while singing and playing them on the guitar. The resulting blues songs in Portuguese are registered on a CD attached to this thesis. Our method is to set them to music while translating them, because the simultaneity of the processes is exactly what might interfere in the linguistic choices, influencing aspects of rhythm, meaning etc. Our hypothesis involves, then, the discussion of how the modes of expression of the popular song, in a given system of language-culture, would determine the results of the translation. That sort of experimentation will hopefully contribute to the Translation Studies by rearranging certain aspects of the text according to the dynamics of vocalization. These results are both academic, in terms of the discussion about translation itself; and artistic, as blues music composed for the Brazilian audience, presenting Langston Hughes, relatively unknown and non-translated in Brazil, in the form of a musical genre which is widely disseminated here.
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14與18個月嬰兒理解他人的溝通意圖 / Fourteen- and Eighteen-Month-Old Infants Understand Others' Communicative Intents簡嘉慧, Chien, Chia Hui Unknown Date (has links)
理解他人的意圖,指的是理解他人的行為背後蘊含著一個希望達成的具體目標,是嬰兒社會認知發展中一項重要的基礎能力,而此能力的發展應具有跨文化一致性。然而,過去國內外研究結果顯示,台灣與西方嬰兒透過非語言線索理解他人溝通意圖的能力有明顯的落差。因此,本研究為了瞭解台灣嬰兒理解非語言線索的溝通意圖之能力,並找出可能導致上述不同研究結果的作業差異原因,將藉由兩項實驗來探討此議題。實驗一以藏物遊戲為實驗派典,觀察14與18個月的台灣嬰兒,在「眼神注視」、「手指指示」及「意圖性語音」三種非語言線索情境中,是否可藉由對線索溝通意圖的理解而找出隱藏的意圖目標物。結果發現14個月的台灣嬰兒,可理解他人「手指指示」線索的溝通意圖,但無法理解「眼神注視」與「意圖性語音」線索的溝通意圖,而18個月的台灣嬰兒則是可理解他人「眼神注視」線索的溝通意圖,但卻無法理解「手指指示」與「意圖性語音」線索的溝通意圖。為了排除藏物容器設計可能造成的干擾因素,實驗二調整了藏物容器裝置,以同樣的實驗方式觀察台灣嬰兒理解他人非語言線索溝通意圖的能力。結果發現,14個月的台灣嬰兒仍只能藉由「手指指示」線索理解他人的溝通意圖,但18個月的台灣嬰兒則已可透過「眼神注視」、「手指指示」及「意圖性語音」三種非語言線索理解他人的溝通意圖。此結果說明,台灣與西方嬰兒透過非語言線索理解他人溝通意圖的能力大致相同,即嬰兒理解他人溝通意圖的能力確實具有跨文化一致性。 / Understanding the intents of others, to understand the existence of specific goals that people wish to achieve from their behaviors, is one of important basic abilities in the social cognitive development of an infant. The developmental time frames of such ability should be the same across cultures. However, previous domestic and overseas studies proposed that the abilities of infants to understand the communicative intents of others through nonverbal cues are different between Taiwanese and western cultures. Hence, in this study, two controlled experiments were conducted to explore the abilities of Taiwanese infants to understand the communicative intents of others through nonverbal cues and the factors of the tasks that may cause different results between domestic and overseas studies. In the first experiment, an experimenter used the cues of gazing, pointing, and intentional vocalization in a hiding-game task to examine whether fourteen- and eighteen-month-old Taiwanese infants could infer communicative intents expressed in the three nonverbal cues and find the hidden objects. Results showed that 14-month-old Taiwanese infants could only follow the cue of pointing to find the hidden objects, but not gazing or intentional vocalization. And 18-month-old Taiwanese infants could find the hidden objects by following only the cue of gazing. To exclude the possible interferences of the target containers, the experimenter used the same procedures and settings in the second experiment as the first one, but modified the containers to clarify the hiding-game task. Results showed that 14-month-old Taiwanese infants could still follow only the cue of pointing to find the hidden object, but 18-month-old Taiwanese infants successfully followed all three types of cues. Based on these findings, the abilities of infants to understand others’ communicative intents by following nonverbal cues are the same in Taiwanese and western cultures. That is, the developmental time frames of the abilities of infants to understand the communicative intents through nonverbal cues are the same across different cultures.
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Weaning and castration in beef calves / Absetzen und Kastration von FleischrindkälbernFarke-Röver, Antje 18 November 2010 (has links)
No description available.
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Participação dos receptores NK-1 dos núcleos basolateral e central da amígdala no comportamento defensivo de ratos / Involvement of NK-1 receptors of the basolateral and central nuclei of the amygdala in the defensive behavior of ratsGabriel Shimizu Bassi 29 June 2012 (has links)
Estudos realizados na última década mostram que a substância P (SP) é um neuromediador importante de estados emocionais e afetivos. A SP tem ação pró-aversiva quando microinjetada na substância cinzenta periaquedutal dorsal (SCPd) através da ativação de receptores neurocininérgicos do tipo NK-1, uma vez que o comportamento defensivo é bloqueado por antagonistas desses receptores. A ativação de receptores NK-1 na SCPd também produz antinocicepção, a qual é considerada parte da reação de defesa. Na sequência desses estudos, este projeto visa investigar o envolvimento dos receptores neurocininérgicos no núcleo central (CeA) e basolateral (BLA) da amígdala na mediação dos estados aversivos gerados e elaborados nessa estrutura prosencefálica que, junto com a SCPd, faz parte do sistema encefálico aversivo. O presente estudo mostrou que a SP e o agonista NK-1 (Sar-Met-SP) promoveram efeitos pró-aversivos no labirinto em cruz elevado somente no CeA, mas não no BLA. Ao contrário da SCPd, não obtivemos qualquer alteração no limiar nociceptivo com a microinjeção de antagonista de receptores NK-1 (Spantide) em ambos os núcleos. O Spantide sozinho não alterou os indicadores de nocicepção e ansiedade. Nenhum tipo de vocalização (audível ou ultrassônica) foi detectado no presente trabalho após a microinjeção de SP ou Sar-Met-SP em ambos núcleos amigdalóides, apesar de relatos de vocalizações ultrassônicas (VUS) após o mesmo tipo de tratamento na SCPd. Os resultados obtidos no presente estudo mostraram que o CeA, mas não o BLA, modula a expressão de comportamentos relacionados ao medo inato através de receptores neurocininérgicos do tipo NK-1. VUS e antinocicepção não parecem participar da reação de defesa elaborada no CeA. A ausência da emissão de VUS nesses núcleos pode indicar que somente estruturas mais antigas do neuroeixo (mesencéfalo e hipotálamo) são responsáveis pela produção de VUS. / A substantial body of evidence obtained in the last decade demonstrated that the Substance P (SP) is an important mediator of the affective and emotional behaviors. SP is a pro-aversive compound when microinjected within the dorsal periaqueductal gray (dPAG). These effects are mediated by the type 1 neurokininergic receptors (NK-1), since the defensive behavior was inhibited by antagonists of these receptors. The activation of NK-1 receptors in the dPAG also produced antinociception and ultrasonic vocalizations (USV). In the sequence of these studies, this study investigated the involvement of neurokinin receptors of the central (CeA) and basolateral (BLA) nuclei of the amygdala in the mediation of the defense reaction. The amigdala together with the dPAG and the medial hypothalamus comprise the encephalic aversive system. The results showed that SP and the NK-1 agonist (Sar-Met-SP) promoted pro-aversive effects in the elevated plus maze test only when microinjected into the CeA, without effect in the BLA. Although SP and the activation of NK-1 receptors induce antinociception in the dPAG, we did not observe any alteration of the nociceptive threshold in the tail-flick test with the NK-1 antagonist (Spantide) injected into both nuclei and any changes of the anxiety parameters in the EPM. No vocalizations (audible or ultrasonic) were detected after treatment with SP or Sar-Met-SP in both amygdaloid nuclei. The lack of emissions of USVs after activation of these nuclei could indicate that only older structures (PAG and hypothalamus) of the neuroaxis are responsible for the production of USVs. The results obtained in the present study show that NK-1 receptors within CeA, but not BLA, modulate the expression of defensive behaviors related to the innate fear. Apparentely USVs and antinociception are not involved in the defensive reactions indiced by activation of NK-1 mechanisms in the CeA.
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Bridging the Gap: Introducing Extended Techniques and Contemporary Notation through Newly Composed Etudes for ClarinetEllard, Luke 05 1900 (has links)
This dissertation aims to address the pedagogical gap in introductory material for contemporary clarinet instruction. Through examining the most prominent contemporary methods for the clarinet, the pedagogical gap is highlighted, particularly regarding material aimed at newcomers and early undergraduate students. To address these needs, a new collection of etudes is proposed, introducing extended techniques and contemporary notation for newcomers to modern music.
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