Thesis (M.S.)--Rhodes University, 2004. / Title from PDF t.p. (viewed on Apr. 30, 2006). Includes bibliographical references (p. 81-92).

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1996. / Includes bibliographical references (leaves 121-151).

Glabrous Brassica napus cv Westar and very hairy AtGL3+ B. napus were transformed using Agrobacterium tumefaciens and either a full length trichome regulatory gene BnTTG1 (isoform 1 coding region called O-TTG1) or an RNAi cassette with 260 bp of a conserved region between isoform I and II (called K-TTG1), each driven by the CaMV 35S promoter. Agronomic and trichome phenotypes were observed in the resulting lines. Transformed lines developed in the glabrous Westar background showed no changes in growth or trichome density and transformation efficiency was similar to that of an empty vector control construct. Over-expression of BnTTG1 in the AtGL3+ B. napus background resulted in low transformant survival and poor seed viability, with the only surviving line O-3-7 being taller than non-transformed lines and with a completely glabrous phenotype. The two knock-down lines with the lowest BnTTG1 expression showed a dramatic increase in trichome density, with longer trichomes and expanded trichome density (up to the 12th leaf in the K-5-8 line) compared to the AtGL3+ hairy background line, which showed increased trichome density only on the first three leaves. Moreover, K-5-8 plants were healthy, with both vegetative and reproductive growth similar to that of Westar non-transgenic control plants under both greenhouse and field conditions. The relative expression of five B. napus primary trichome regulatory genes and AtGL3 was measured in three different tissues of B. napus Westar, and the AtGL3+, K-5-8 and O-3-7 transgenic lines. Over-expression of AtGL3 resulted in changes in the expression of BnGL3, BnGL2 and BnTRY. Manipulation of BnTTG1 levels also resulted in changes in expression of these three genes in addition to AtGL3. AtGL3+ plants and O-3-7 also showed increased red pigment accumulation in several above ground vegetative tissues including cotyledons, hypocotyl and leaves, whereas the K-5-8 line (knock down of TTG1) had less anthocyanin in the same tissues. The level of anthocyanin accumulation corresponded to the relative expression of the three primary anthocyanin regulatory genes BnDFR, BnANS and BnGST. In a laboratory bioassay, diamondback moth (DBM) adults (Plutella xylostella) laid more eggs on hairy leaves of K-5-8 than glabrous Westar. However, more feeding damage from young DBM larvae was observed on Westar leaves than K-5-8 in both choice and no-choice feeding assays. In a field test comparing Phyllotreta flea beetle feeding, the hairy K-5-8 leaves showed between a 30-50% reduction in feeding over four ratings on 14 to 28 day old seedlings. Curiously, the glabrous cotyledons of the two hairy lines (AtGL3+ and K-5-8) proved to be more resistant than wild type B. napus Westar cotyledons from Helix XTra® insecticide-treated or non-treated seed. These data support the introduction of AtGL3 and the knockdown of BnTTG1 to induce a dense trichome phenotype, into otherwise glabrous B. napus, resulting in an increased host plant resistance to crucifer insects, without agronomic penalties.

Thesis (M. Environmental Science))--North-West University, Potchefstroom Campus, 2007.

Cruciferous crops comprise one of the major crop divisions and contribute significantly to global crop production. Insect pests associated with cruciferous crops have the potential to destroy harvests. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and cabbage aphids, Brevicoryne brassicae (L.) and Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae) are considered the most important cabbage pests in South Africa. Pest control in cabbage is still heavily reliant on use of insecticides even though insecticide application usually result in increased pest status of P. xylostella. Use of integrated pest management (IPM) strategies may yet prove invaluable as a means of suppressing the more serious cabbage pests. Global interest in cultural control strategies, that includes intercropping of unrelated crop plants, has been rekindled because of problems experienced with pesticide use. A study that involved the planting of two field trials with cabbage and carrot plants in different ratios, as a substitutive intercropping system, was undertaken during the 2002/03 and 2003/04 planting seasons. Field trials had five treatments viz. control (cabbage monoculture sprayed with mercaptothion), cabbage monoculture (without chemical application), 1:1 intercropping (cabbage:carrots), 1:3 intercropping and 1:5 intercropping, replicated six times in a randomized block design. Insects were sampled weekly by removing the third-youngest open leaf, from one plant per plot (six plants per treatment). Feeding damage to cabbage leaves was assessed visually by rating 14 plants per plot according to a numeric scale based on damage severity. P. xylostella and aphid parasitism levels were determined. Prominence values were calculated for P. xylostella larvae and aphids for each treatment per sampling date. P. xylostella infestations and parasitism levels were significantly (P ≤ 0.05) lower in control treatments. Intercropping ratio did not have a significant impact on pest infestations. P. xylostella infestations and parasitism on intercropping treatments did not differ significantly (P > 0.05) from that on monoculture plots (except for 1:5 intercropping during the 2003/04 season). Cotesia plutellae (Hymenoptera: Braconidae) was the dominant parasitoid and parasitism levels were significantly correlated with P. xylostella infestation levels. Prominence values showed a decline in P. xylostella infestations on intercropping treatments towards the last sampling date during both seasons. Feeding damage severity was lowest on the control treatment but similar for other treatments for both seasons. Aphid infestations were significantly (P ≤ 0.05) lower on the control treatment, but did not differ significantly between intercropping treatments. Aphid prominence values indicated possible suppression of infestations at low population levels during the early part of the season. Aphid parasitism was low during both seasons. It seems more likely that the reduction in insect infestation levels reported from the literature are related more to spatial arrangement of plants than cropping ratios. The observed suppression of aphid infestation levels on intercropped plots early in the season may be valuable in reducing the need for early chemical applications. The limiting of early insecticide application against aphids may result in a reduced pest status of P. xylostella, due to the conservation of C. plutellae populations. / Thesis (M. Environmental Science))--North-West University, Potchefstroom Campus, 2007.

Cruciferous crops comprise one of the major crop divisions and contribute significantly to global crop production. Insect pests associated with cruciferous crops have the potential to destroy harvests. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and cabbage aphids, Brevicoryne brassicae (L.) and Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae) are considered the most important cabbage pests in South Africa. Pest control in cabbage is still heavily reliant on use of insecticides even though insecticide application usually result in increased pest status of P. xylostella. Use of integrated pest management (IPM) strategies may yet prove invaluable as a means of suppressing the more serious cabbage pests. Global interest in cultural control strategies, that includes intercropping of unrelated crop plants, has been rekindled because of problems experienced with pesticide use. A study that involved the planting of two field trials with cabbage and carrot plants in different ratios, as a substitutive intercropping system, was undertaken during the 2002/03 and 2003/04 planting seasons. Field trials had five treatments viz. control (cabbage monoculture sprayed with mercaptothion), cabbage monoculture (without chemical application), 1:1 intercropping (cabbage:carrots), 1:3 intercropping and 1:5 intercropping, replicated six times in a randomized block design. Insects were sampled weekly by removing the third-youngest open leaf, from one plant per plot (six plants per treatment). Feeding damage to cabbage leaves was assessed visually by rating 14 plants per plot according to a numeric scale based on damage severity. P. xylostella and aphid parasitism levels were determined. Prominence values were calculated for P. xylostella larvae and aphids for each treatment per sampling date. P. xylostella infestations and parasitism levels were significantly (P ≤ 0.05) lower in control treatments. Intercropping ratio did not have a significant impact on pest infestations. P. xylostella infestations and parasitism on intercropping treatments did not differ significantly (P > 0.05) from that on monoculture plots (except for 1:5 intercropping during the 2003/04 season). Cotesia plutellae (Hymenoptera: Braconidae) was the dominant parasitoid and parasitism levels were significantly correlated with P. xylostella infestation levels. Prominence values showed a decline in P. xylostella infestations on intercropping treatments towards the last sampling date during both seasons. Feeding damage severity was lowest on the control treatment but similar for other treatments for both seasons. Aphid infestations were significantly (P ≤ 0.05) lower on the control treatment, but did not differ significantly between intercropping treatments. Aphid prominence values indicated possible suppression of infestations at low population levels during the early part of the season. Aphid parasitism was low during both seasons. It seems more likely that the reduction in insect infestation levels reported from the literature are related more to spatial arrangement of plants than cropping ratios. The observed suppression of aphid infestation levels on intercropped plots early in the season may be valuable in reducing the need for early chemical applications. The limiting of early insecticide application against aphids may result in a reduced pest status of P. xylostella, due to the conservation of C. plutellae populations. / Thesis (M. Environmental Science))--North-West University, Potchefstroom Campus, 2007.

The diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is a cosmopolitan insect pest of Brassica crops. In South Africa, there are no action thresholds for its chemical control which makes it difficult for growers to make informed decisions on when to apply insecticides and how frequently to apply them in order to achieve optimal crop yield. To contribute towards optimum application of insecticides against P. xylostella, this study compared the impact of weekly and bi-weekly applications of a selective insecticide Dipel® (Bacillus thuringiensis Berliner var. kurstaki) applied at 250 g/ha, and a broad-spectrum insecticide Dichlorvos (an organophosphate) applied at 1 ml/L against biological control (Control) on the pest population density on cabbage during October– December 2011 and March–May 2012. The use of both selective and broad-spectrum insecticides for experiments enables us to understand if efforts to optimise cabbage yield depend mainly on effective suppression of P. xylostella densities. Furthermore, investigations were carried out to determine the impact of these chemicals on parasitism rates of P. xylostella and species richness of its primary parasitoids. During the October–December 2011 growing season, the lowest infestation of P. xylostella occurred on cabbage plots that received weekly application of Dipel and the highest on untreated control plots. Cabbage weights were negatively related to infestation levels, implying that weekly application of Dipel yielded bigger cabbage heads. During March– May 2012, P. xylostella infestations were again higher on the control followed by weekly and bi-weekly treatments of Dichlorvos, then weekly and bi-weekly applications of Dipel. Despite the significant differences observed, infestation levels were much lower (< 1 P. xylostella per plant on average) in all treatments during this season. Consequently no significant differences in cabbage weights were observed among the treatments. The lower infestation levels were attributed to higher parasitism levels (≥50 %), especially during the early stages of crop development. A total of four parasitic Hymenoptera species were recorded from P. xylostella larvae and pupae during October–December 2011, while three species were recorded during March– May 2012. However, Cotesia vestalis (Haliday) (Braconidae) accounted for >80 % of total parasitism levels in all treatments. Parasitism levels were not significantly different among the treatments in both seasons. Parasitoid species richness was highest on the control. Although two parasitoid species were recorded in all Dipel and Dichlorvos treatments during October–December 2011, only one parasitoid species was recorded in the Dipel treatments during March–May 2012 compared to two species in Dichlorvos treatments. Although weekly applications of Dipel ensured good yield and crop quality during October–December, weekly applications of the chemical did not lead to better quality crop during March–May crop growing season. Thus, it is not necessary to apply insecticides during periods in which natural mortality of P. xylostella is high due to parasitoids. Since P. xylostella abundance was a determining factor of crop quality, these results imply that insect pest management should focus mainly on suppressing its numbers. Furthermore, there was no evidence that application of either insecticide type had a negative impact on parasitism rates of P. xylostella. The lower parasitoid species richness on Dipel treated plots was the consequence of its higher efficiency in suppressing the pest population which substantially reduced availability of potential hosts for parasitoids, hence only the efficient C. vestalis was recorded at low host densities / Agriculture and  Animal Health / M.Sc. (Agriculture)

The diamondback moth (Plutella xylostella L.) is a major pest of crucifer crops worldwide. It is a particularly important problem in Southeast Asia where climatic conditions favor a short generation time and rapid proliferation. In countries such as Thailand, the diamondback moth has apparently become resistant to all classes of insecticides, even the newest ones. The first part of this thesis is the development of a computer database of all the available information on diamondback moth toxicology. About 1,550 records were extracted from more than 235 published or unpublished documents. These records include information on the geographical location of experiments, the insecticide evaluated, the testing method and the results observed. Most records in the database are from the last decade, and are predominantly from Taiwan, Thailand, the USA and Japan. Trends in the use of various classes of insecticides can easily be followed. Thirty three records have been found of insecticide resistance of more than a thousandfold. An the known mechanisms of resistance are found, and the diamondback moth has become resistant to all the classes of insecticides, including Bacillus thuringiensis but excluding abamectin. Case studies made possible by the use of the database showed a rapid succession in the use of 29 insecticides in the last 26 years in a single country (Thailand). Although this rapid succession is usually ascribed to the development of resistance, a case study on the regional differences in resistance to five insecticides showed marked variations in the level of resistance over distances of 20 miles or more. The possibility of managing insecticide resistance at the local level, already suggested by studies in Hawaii, is thus supported by this analysis. In the second part of this thesis, the toxicity of Bacillus thuringiensis var. aizawai and of the bacterial toxin abamectin was tested by two bioassay techniques on populations of the diamondback moth from two Chinese kale fields at locations in Thailand at which the highest levels of resistance to most insecticides have been reported. The leaf-dip bioassay was found to be superior to a residue-vial assay or to the FAO-recommended assay. More than 70-fold resistance to Bacillus thuringiensis var. aizawai was observed in the populations of DBM collected from these two fields, suporting the theory that resistance resulted in the control failures reported by growers. Resistance did not explain control failures with abamectin as no resistance to this compound could be detected. The third part of this thesis analyzes the intraplant distribution of the diamondback moth larvae in an effort to explain the control failures with abamectin on Chinese kale. Extensive sampling of the various larval instars of the diamondback moth revealed that a majority of second instar larvae were sheltered on the young folded leaves of Chinese kale. The majority of fourth instar larvae and pupae were found on mature (lower) leaves. Inefficient spraying practices observed in the field with high volume, low pressure hydraulic pump sprayers may allow the young larvae to escape pesticide exposure possibly explaining control failures in the absence of physiological resistance. This study shows that resistance in the diamondback moth is a worldwide problem of major proportions. The rapid development of resistance to Bacillus thuringiensis in the field is of practical concern. Resistance management programs in developing countries should include mechanisms to optimize insecticide delivery to the protected habitats of diamondback moth on Chinese kale. / Graduation date: 1992

The diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is a cosmopolitan insect pest of Brassica crops. In South Africa, there are no action thresholds for its chemical control which makes it difficult for growers to make informed decisions on when to apply insecticides and how frequently to apply them in order to achieve optimal crop yield. To contribute towards optimum application of insecticides against P. xylostella, this study compared the impact of weekly and bi-weekly applications of a selective insecticide Dipel® (Bacillus thuringiensis Berliner var. kurstaki) applied at 250 g/ha, and a broad-spectrum insecticide Dichlorvos (an organophosphate) applied at 1 ml/L against biological control (Control) on the pest population density on cabbage during October– December 2011 and March–May 2012. The use of both selective and broad-spectrum insecticides for experiments enables us to understand if efforts to optimise cabbage yield depend mainly on effective suppression of P. xylostella densities. Furthermore, investigations were carried out to determine the impact of these chemicals on parasitism rates of P. xylostella and species richness of its primary parasitoids. During the October–December 2011 growing season, the lowest infestation of P. xylostella occurred on cabbage plots that received weekly application of Dipel and the highest on untreated control plots. Cabbage weights were negatively related to infestation levels, implying that weekly application of Dipel yielded bigger cabbage heads. During March– May 2012, P. xylostella infestations were again higher on the control followed by weekly and bi-weekly treatments of Dichlorvos, then weekly and bi-weekly applications of Dipel. Despite the significant differences observed, infestation levels were much lower (< 1 P. xylostella per plant on average) in all treatments during this season. Consequently no significant differences in cabbage weights were observed among the treatments. The lower infestation levels were attributed to higher parasitism levels (≥50 %), especially during the early stages of crop development. A total of four parasitic Hymenoptera species were recorded from P. xylostella larvae and pupae during October–December 2011, while three species were recorded during March– May 2012. However, Cotesia vestalis (Haliday) (Braconidae) accounted for >80 % of total parasitism levels in all treatments. Parasitism levels were not significantly different among the treatments in both seasons. Parasitoid species richness was highest on the control. Although two parasitoid species were recorded in all Dipel and Dichlorvos treatments during October–December 2011, only one parasitoid species was recorded in the Dipel treatments during March–May 2012 compared to two species in Dichlorvos treatments. Although weekly applications of Dipel ensured good yield and crop quality during October–December, weekly applications of the chemical did not lead to better quality crop during March–May crop growing season. Thus, it is not necessary to apply insecticides during periods in which natural mortality of P. xylostella is high due to parasitoids. Since P. xylostella abundance was a determining factor of crop quality, these results imply that insect pest management should focus mainly on suppressing its numbers. Furthermore, there was no evidence that application of either insecticide type had a negative impact on parasitism rates of P. xylostella. The lower parasitoid species richness on Dipel treated plots was the consequence of its higher efficiency in suppressing the pest population which substantially reduced availability of potential hosts for parasitoids, hence only the efficient C. vestalis was recorded at low host densities / Agriculture and  Animal Health / M.Sc. (Agriculture)

Thesis (Ph.D.)--University of Adelaide, Dept. of Applied and Molecular Ecology, 2001. / Includes bibliographical references (leaves 204-230).