Thesis (M.Sc.(Entomology ))--University of Pretoria, 2002. / Includes abstract in English. Includes bibliographical references.

Thesis (Ph. D.)--Swedish University of Agricultural Sciences, 2001. / Thesis statement in Swedish and English abstract inserted. Includes bibliographical references.

The biology and ecology of the endocoprid dung beetles of the Aethiopian zoogeographic region has been studied in the field and laboratory. Work has centred mainly on Oniticellus planatus and O. formoaus although infomation has also been supplied on O. egregius, O. pictus and Tragiscus dimidiatus. The taxonomy of Onticellus (s.str.) and Tragiscus has been discussed with the recommendation that O. planattus and O. pseudaplanatus be synonymised. It has been suggested that endocoprid distribution is chiefly controlled by temperature, rainfall and the density of suitable large dung masses. On the basis of distribution it has been suggested that the taxonomic status of the subspecies of O. pictus should be reviewed. Endocoprids (genus Oniticellus (s.str.) are found throughout the warmer regions of Africa and Asia. Tragiscus is found only in Africa. Methods are described for monitoring endocoprids in the field, for distinguishing freshly emerged from older specimens, and for breeding endocoprids under laboratory conditions. It has been shown that endocoprids breed within or just beneath large dung masses unlike most other members of the Scarabaeinae which actively bury dung and construct the their broods at depth in the soil. It has been shown that there is a restriction of breeding sites available to endocoprids due to the removal of dung by the Scarabaeinae beetles and the limited space available within dung pads. This has resulted in low endocoprid population numbers, a disadvantage, which has been countered by highly specialised but opportunistic breeding habits. The requirements of dung plasticity have limited the time available for breeding activity by most endocoprids (excluding O. egresius) so that both the reproductive system and the reproductive behaviour (cf. O. planatus) are geared to rapid egg and brood production. Large numbers of follicles are able to be produced over a short period. In the probable absence of feeding, this has possibly been allowed by the involvement of a well developed fat body. The broods are tended by the parent female during larval development and the follicles are gradually resorbed, probably to prevent starvation. Ovarian recovery is rapid once feeding recommences. Behavioural mechanisms have developed which boost the number of broods constructed and the rapidity with which they are produced. The duration of endocoprid colonisation and the timing of arrival at dung is shown to be related to the rate of pad desiccation which is chiefly controlled by temperature and degree of dung removal by other dung beetles. Precipitation may also play a part. Predation by vertebrates and other insects has been noted and a number of mechanisms are described which are probably protective. Two insect parasitoids of O. formosus larvae have been recorded. The potential use of endocoprids in the Australian dung and fly control project has been discussed and it is recommended that their introduction be considered of low priority. It is suggested that the endocoprids evolved from Euoniticellus type ancestors and that their behaviour complex nidification developed in response to harsh environmental factors, chiefly the danger of desiccation and competition for dung with other dung beetles. From a consideration of brood and brood chamber construction it is suggested that O. egresius is a relatively unspecialised endocoprid which may retain dry season aestivation and has not evolved much further than its probable Euoniticellus type ancestor. The broods are coated in clay and abandoned soon after construction. Greater specialisation is shown by O. planatus and O. formosus which exhibit brooding behaviour, increasing longevity, more specialised brood protection, increased potential fecundity and continuous activity throughout the year. O. formosus shows greater specialisation in brood production than O. planatus. Greatest specialisation is found in T. dimidiatus which exhibits a precise brooding period and low egg production. Brood construction is of a similar degree of specialisation to that of O. formosus. Activity is also continuous throughout the year. The comparative biology of the five southern African endocoprids is summarised in Table 35. Summary, p. 129-131.

Ivermectin also called the wonder drug has been used over the years to control internal and external parasites in livestock. In humans it has been used for the control of several neglected tropical diseases. With regards to efforts to control malaria, mass drug administration (MDA) of ivermectin in humans and livestock has been considered as a potential tool. This is due to challenges in malaria preventive strategies such as insecticide resistance in mosquitoes, shift in their behaviors and residual transmission. Ivermectin reduces malaria transmission by targeting the mosquito nervous system resulting in their death. Ivermectin and its metabolites could have great impacts on the environment as well as human and health. In temperate settings, impacts of ivermectin in the environment were studied due to intense use of the drug in livestock. There is also a necessity to study effects of ivermectin in the tropics before MDA for malaria vector control. Despite its great potential, previous studies done recorded toxicity and sensitivity of the drug to most arthropods mainly dung organisms because ivermectin is released in dung at high concentrations for the case of livestock. With dung organism activity being affected the dung decomposition process is disrupted, cattle avoid these grazing areas leading to financial losses. In the tropics where there is a high number of malaria cases, there is no information on the impacts of the drug in the environment. We placed standardized dung pats from ivermectin-treated and control cattle to determine the effect of ivermectin on dung degradation and dung fauna in Tanzania. For the dung degradation study, at 15, 30 and 45 days post placement, we observed a total of 220 dung pats in the field. We measured termite colonization; wet weight of the entire pat; water content; dry weight of the 10 g subsamples and organic matter from subsamples and the whole pat. For the dung fauna study, we collected fresh dung 3, 10 and 29 days post treatment and put the pats out in the field to be colonized by insects before being transferred to emergence traps. We also did a semi field study where we collected dung pats 1,2,3 and 5 days post treatment to obtain larvae counts. We qualitatively assessed insect larvae activity in the field experiment and observed and counted larvae in the semi field study. We found that termites colonized pats from cattle treated with ivermectin more readily compared to controls (p < 0.001). Compared to control pats, the treated pats' wet weight decreased more slowly on day 15 (p < 0.001), day 30 (p < 0.001), and on day 45 (p = 0.037). Percent dry weight increased over time and similarly between the treatments as water content decreased. Organic matter of the 10g sub samples was similar between the treatments. Total organic matter in the whole pats showed significant differences on day 15 (p < 0.001), and day 30 (p = 0.003), but not on day 45 (p = 0.291). Qualitatively, we observed that pats from treated cattle had less insect larvae activity as compared to controls in the field study. In the semi field study, we counted less larvae in the pats from ivermectin-treated cattle than in the control pats (p < 0.001). Our results indicate that ivermectin and its residues affect dung degradation and dung fauna in tropical savanna settings, and the environmental safety may be at risk upon mass drug administration in livestock. / Master of Science in Life Sciences / Ivermectin is a drug commonly used in livestock and humans to control most parasitic infections. Malaria is a disease transmitted by female Anopheles mosquitoes and prevalent in tropical regions mostly the sub-Saharan Africa. There are efforts worldwide to reduce transmission of malaria such as the used of insecticide treated bed nets as well as use of repellents and spraying insecticides indoors. These efforts are challenged by insecticide resistance in mosquitoes, change in mosquito behavior as well as remaining malaria cases after such interventions are applied. With such challenges comes a need to use ivermectin which has the ability to kill mosquitoes. Despite its great potential, evidence from temperate regions record that ivermectin affects the environment by decreasing dung insect activity, affecting the developmental process and causing a delay in dung decomposition. These effects cause cattle to avoid such pasture areas, resulting in an increase in pest pressure and affecting the economy in general. In the tropics where malaria is prevalent and plans are in place to use ivermectin in mass drug administration for mosquito control, there is a need to look at the environmental impact of the drug. We placed 1 kg dung pats from ivermectin-treated and control cattle to determine the effect of ivermectin on dung degradation and dung fauna in Tanzania. For the dung degradation study; at 15, 30 and 45 days after pats were placed in the field, we observed a total of 220 dung pats. We measured termite colonization; wet weight of the entire pat; water content; dry weight of the 10 g subsamples and organic matter from subsamples and the whole pat. For the dung fauna study, we collected fresh dung 3,10 and 29 days post treatment and put the pats out in the field to be colonized by insects before being transferred to emergence traps. We also did a semi field study where pats were collected 1,2,3 and 5 days post treatment to obtain larvae counts. We qualitatively assessed insect larvae activity in the field experiment and observed and counted larvae in the semi field study. We found that termites colonized pats from cattle treated with ivermectin more readily compared to controls, and treated pats' wet weight decreased more slowly. Dry weight of dung increased as water content decreased with no differences between the treatments. Organic matter of the subsamples did not differ in the treatments but the average mass of organic matter of the individual dung pats decreased slower in ivermectin-treated pats. Qualitatively, we observed that pats from treated cattle had less insect larvae activity as compared to controls in the field study. In the semi field study, we counted more larvae in control pats than the ivermectin pats. Our results indicate that ivermectin and its residues affect dung degradation and dung fauna in tropical savanna settings, and the environmental safety may be at risk upon mass drug administration setting.

No description available.

Thesis (M.Sc.(Zoology)) -- University of Pretoria, 1999. / Includes bibliographical references.

Thesis (Ph.D.)(Entomology)--University of Pretoria, 2005. / Title from opening screen (viewed March 20, 2006). Includes summary. Includes bibliographical references.

Please read the abstract in the section 00front of this document / Dissertation (MSc (Entomology))--University of Pretoria, 2006. / Zoology and Entomology / unrestricted

Please read the abstract in the section 00front of this document / Thesis (PhD (Entomology))--University of Pretoria, 2006. / Zoology and Entomology / unrestricted

This dissertation was submitted to the Faculty of Science, University of the Witwatersrand, in fulfillment of the requirements for the degree of Master of Science / Dung beetle feeding is still not a widely understood behaviour. Feeding on the waste of other animals creates a challenge of acquiring the necessary nutrients for growth, development and for survival. Because of their soft mouthparts, it has always been thought that adult dung beetles cannot chew the hard parts of dung but only select smaller particles to feed on. Dung beetle larvae on the other hand can chew harder components, but where they get the nutrients required for development has not yet been discovered. The aim of this study was to discover which dung particles adult dung beetles select when feeding, and when creating a nest for their offspring, and also how much they can alter the dung nutrient content in the process. Foregut and hindgut contents were dissected out of the dung beetle Scarabaeus goryi, and their contents compared with untreated dung from the cow and the excreta of adult beetles. Brood balls and maternal gifts of Euoniticellus intermedius were also dissected. Nitrogen and carbon content, the carbon to nitrogen ratio, and results of the carbon and nitrogen stable isotopes were compared between all samples. When feeding dung beetles selected particles which were bigger than 800 μm in diameter and had three times more nitrogen than raw dung, and used particles over 2000 μm in size in the maternal gift packed for their offspring. Nitrogen content in the selected dung was increased from 1.40 % to 5.14% by adult dung beetle feeding and up to 2.62% in dung selected for the larvae to feed on. The carbon to nitrogen ratio also decreased when feeding adults and in the brood balls made for the larvae to feed on. Both carbon and nitrogen were absorbed from ingested dung during digestion. The heavier nitrogen isotope was selected for when feeding. The heavier nitrogen isotopes and the lighter carbon isotope were selected for during absorption. Dung beetles increase the nitrogen content of the dung on which they feed by careful selection of particles of a certain size and nitrogen content, which is probably the limiting nutrient when feeding and nesting. The increase in the nitrogen content is not only from the change in particle size but also in selection of particles with higher nitrogen content. / MT2018