Typescript (photocopy). / Digitized by Kansas Correctional Industries

ITC/USA 2006 Conference Proceedings / The Forty-Second Annual International Telemetering Conference and Technical Exhibition / October 23-26, 2006 / Town and Country Resort & Convention Center, San Diego, California / A novel Automation System for the Flight Test Laboratory (SALEV) is developed in full compliance with EA-4/02 Standard (i.e. Expression of the Uncertainty of Measurement in Calibration) to compute the uncertainty of the measurement at the calibration laboratory of the Flight Tests Group (GEEV). The GEEV performs flight test campaigns to certificate and/or develop aircrafts and its systems. Then, flight tests instrumentation (FTI) systems are developed and installed in the test bed. The FTI data acquisition complies with IRIG Standard. The FTI is composed by a data acquisition system, which performs signal conditioning, sampling and quantization of all measurements provided by a set of transducers. All parameters are coded in a PCM format and represented in a non-dimensional numerical form (i.e. counts).To allow the establishment of a relation between the non-dimensional form and the physical quantity, a calibration process is carried out to provide the coefficients of a calibration curve. This process is also used to determine the systematic and random errors (i.e. the uncertainty). The accuracy and reliability of calibration process should comply with the requirements, which are customized for each flight test campaign. The satisfactory performance of the SALEV calibration process is achieved by automation in all steps. The SALEV development is presented, which includes the following steps: · Database definition; · Study of all steps and parts that forms the calibration process (i.e. from transducer to final uncertainty determination) to determine its associated uncertainties; · Automation of the entire calibration process (including the process itself up to the effective control of standard and instruments); · Development of algorithms to compute the uncertainty compliant with EA 4/02; and · System validation in compliance with ISO/IEC 17025. As result of the SALEV operation, it could be verified that measurement quality was improved, and the required time for calibration was substantially reduced. Also the standardization of this process allows failures forecast due to aging of systems parameters (i.e. bias).

During peak lactation female mammals reach a limit in their maximal sustained energy intake (SusEI). The causes of such limits is disputed. In this thesis, I examined the causes of the limits on SusEI at peak lactation, and then explored the consequences of such limits for reproductive performance. Finally I tested a possible physiological mechanism that may underpin the trade-off between reproduction and somatic protection (the oxidative stress theory). To answer these questions, I studied reproductive performance and oxidative stress in two lines of mice previously selected for high and low food intake (MH and ML, respectively). I found that these mice reached a plateau in their food intake around day 13 of lactation. In support of the heat dissipation limits theory, reproductive performance in the MH mice was significantly higher than that of the ML mice. Oxidative damage is expected to be higher among lactating individuals. Moreover, lactating mice with greater reproductive performance are also predicted to experience more oxidative damage. By measuring multiple-markers of oxidative damage and protection in different tissues, I found that lactation resulted in reduced oxidative damage in both brain and serum. Additionally, it did not increase oxidative damage to proteins and DNA in liver. Moreover, multiple measures of oxidative stress in the mammary gland were not significantly different between mice with different reproductive effort. Furthermore, I found that lactating mice with greater reproductive performance (litter size and litter mass) had reduced protein damage in their livers and upregulated protection (HSP70) in their brains. These results were inconsistent with the oxidative stress theory. Finally, I employed a novel approach to assess oxidative stress differences with metabolomics analysis. I found that lactation resulted in significant differences in the metabolome. By focusing on the metabolites that are related to vi oxidative stress, I found that most of these metabolites measured in livers and brains were not affected by lactation which provides more evidence against the oxidative stress theory. My results provide support for the heat dissipation theory as a mechanism explaining the limits on reproductive performance. Moreover it provides comprehensive information against oxidative stress as a mediator of life history trade-offs.

The Siberian hamster (Phodopus sungorus) undergoes a suite of physiological changes in response to short day (SD) photoperiod which includes a marked reduction in body mass (up to 40%). This altered physiology can be reversed by a return to long day (LD) photoperiod and is driven by changes hypothalamic gene expression. Additionally, stimulation of weight regain occurs through spontaneous exercise when hamsters are provided with a running wheel (RW), despite intact photoperiod appropriate hypothalamic gene expression. The foundation hypothesis for this investigation was that the change in body weight in both paradigms is underpinned by an alteration of the growth hormone (GH) axis. Pasireotide, a somatostatin agonist, was utilised to inhibit GH secretion from the pituitary in both paradigms. Measurement of body mass, mass of internal organs, body composition by magnetic resonance imaging, hormonal analysis and in situ hybridization were used to determine the effect of a blockade of GH secretion by pasireotide. Pasireotide suppressed the GH axis in Siberian hamsters; with reduced circulating insulin-like growth factor-1 and altered hypothalamic gene expression of somatostatin (srif) and growth hormone – releasing hormone (ghrh) consistent with an inhibition of pituitary GH secretion. Pasireotide treatment inhibited RW and LD stimulated growth, and when administered to LD hamsters caused weight loss in a similar manner to that which occurs in SD and accompanied by testicular atrophy. In addition, pasireotide increased the incidence of torpor and increased bout length of this hypometabolic state in sedentary SD hamsters. In conclusion, evidence is provided for the hypothalamic – pituitary – growth hormone axis in the determination of photoperiod and RW induced body weight changes. Furthermore, the data show evidence for a novel muscle – brain pathway and evidence for a neuroendocrine pathway involved in torpor induction.

published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy

published_or_final_version / Pathology / Doctoral / Doctor of Philosophy

published_or_final_version / Obstetrics and Gynaecology / Master / Master of Philosophy

No description available.

No description available.

The joint may be considered a weak point along the pipe and can have a major impact on pipe performance. However, little research has been conducted in regard to joint design. To improve current structural design criteria, this thesis presents the findings and conclusions of experimental and computational studies of the effects of longitudinal bending on joints in rigid (reinforced concrete) and flexible (corrugated steel and thermoplastic) pipes. Solutions for expected shear force, longitudinal bending moment and rotation across the joint for rigid and flexible pipes are formulated for use in structural design of pipe joints. Exact algebraic solutions for rigid pipes are derived using the beam-on-elastic-spring approximation. The formulations for flexible pipes are developed assuming that the two pipes are very long, and that the response is not affected by either the location or characteristics of those other joints. The flexible pipe design equations are developed using various closed form solutions for beams on elastic foundations developed by Hetényi (1948). Parametric studies are then presented where the key factors controlling the behaviour are examined, and the comparisons to recent experimental measurements show that the joint rotation calculated using the equations and a value of soil stiffness proposed for use in design are generally reasonable and conservative compared with the laboratory tests. To measure the capacity of the joint to accommodate the demands, a pipe joint testing frame has been designed to facilitate joint characterization experiments. Shear tests and articulation (rotation) tests have been conducted using this testing frame to examine the shear force capacity, longitudinal bending moment capacity of moment-transfer joints, and rotational characteristics of joints. The shear test results show that the joints of PVC pipe and corrugated steel pipe have similar shear stiffness while the reinforced concrete pipe joint is significantly stiffer. The results of the articulation testing indicate that the rotational capacities of the three joint systems are similar in general. Design of rotational capacity of these joints is likely dominated by considerations of assembly in the field, rather than the rotational capacity that is needed once the pipes are installed. / Thesis (Master, Civil Engineering) -- Queen's University, 2013-01-31 15:33:41.058