Microspeakers can overheat and break if not monitored and regulated. This monitoringis usually done by adding a pilot tone that introduces energy to the signal.A problem with this approach is the slow update rate of the temperature estimate.This in combination with a fast temperature rise could result in an audible regulationof the input. By simulating the voice coil temperature these problems couldbe mitigated. In this thesis, two existing grey box models and one novel black boxmodel are estimated for different speakers and evaluated using different signals.The results are promising and indicate that all models can estimate the voice coiltemperature with a mean error below one degree. The tests show that a correctinitialization of the model is crucial. Therefore the suggestion to Cirrus Logic,who hosted this thesis project, is to combine a feedforward model with eithertemperature sensor data from the mobile device or a pilot tone. / Mikrohögtalare kan överhettas och gå sönder ifall temperaturen inte övervakasoch regleras vid behov. Denna övervakning sker med hjälp av en pilotton somtillför energi till högtalarens insignal. Ett problem med denna lösning är att övervakningenär relativt långsam. Detta gör att en snabb temperaturökning kan geen oönskad hörbar reglering av insignalen. Genom att modellera spolens temperaturkan detta problem hanteras. I detta examensarbete tas två fysikaliska modelleroch en konfektionsmodell fram och testas på olika högtalare och signaler.Resultaten är lovande och visar att alla modeller kan skatta spoltemperaturenmed ett medelfel under en grad. Utvärderingen visar att initiering av modellensstarttemperatur är viktig. Därför är förslaget till Cirrus Logic att kombinera en simuleringsmodellsom initieras med antingen temperatursensordata från mobileneller med hjälp av en pilotton.

Das Synaptonemalkomplexprotein SYCP1 ist eine Strukturkomponente des Synaptonemalkomplexes (SC) von Saeugern, einer meiosespezifischen Struktur, die wesentlich fuer die Synapse, Rekombination und Segregation homologer Chromosomen ist. Der SC besteht aus zwei lateralen Elementen (LEs) und einer zentralen Region (CR), in deren Mitte das zentrale Element (CE) liegt. Dabei sind die LEs den Achsen der homologen Chromosomen aufgelagert und werden in der CR durch Transversalfilamente (TFs) mit dem CE verbunden. Im Protein SYCP1 (125 kDa) flankieren zwei nicht-helikale terminale Domaenen eine ausgedehnte zentrale „Coiled-Coil“-Domaene. Fuer diese Domaene wird angenommen, dass sie die die Kluft zwischen LEs und CE ueberbrueckt, wobei die C-Termini in den LEs verankert sind und die N-Termini im CE lokalisiert wurden. Um die molekulare Architektur des SC besser zu verstehen und die Bedeutung von SYCP1 für die Zusammenlagerung des SC aufzudecken, wurden die Polymerisationseigenschaften von SYCP1 erforscht. Dazu wurde das Protein in somatischen Zellen exprimiert. In diesem experimentellem Ansatz polymerisierte SYCP1 autonom zu filamentoesen Strukturen, welche sich auf ultrastruktureller Ebene als alternierende elektronendichte Balken offenbarten, die ueber TFs verbunden waren. Dieser Aufbau glich parallel aneinander gereihten Stapeln von SCs, so genannten Polykomplexen (PCs). Die Analyse der Orientierung der SYCP1 Molekuele innerhalb der PCs erwies, dass diese hochorganisiert vorliegen und die Organisation von SYCP1 innerhalb von PCs und SCs identisch ist. Folglich kann sich SYCP1 sogar in Abwesenheit anderer SC-Proteine zu Strukturen zusammenlagern, die der CR entsprechen und muss dementsprechend beim Aufbau der CR des SC den grundlegenden Faktor darstellen. Für eine genauere Analyse wurden ausgewaehlte Mutanten von SYCP1 exprimiert. Moleküle mit modifizierter Laenge der zentralen alpha-helikalen Domaene resultierten in der Bildung von PCs mit veränderter Weite der CR. Dies beweist, dass die „Coiled-Coil“-Domaene den Abstand der CR eines PC bestimmt und impliziert dieselbe Funktion in der SC-Bildung. Darueber hinaus wurde gezeigt, dass SYCP1 Molekuele mit Deletion des nicht-helikalen N-Terminus immer noch in der Lage sind, PCs zu bilden, diese Eigenschaft aber stark eingeschraenkt ist. Das bezeugt die Bedeutung des N-Terminus sowohl in der PC-Bildung als auch im Aufbau des CE von SCs, weist aber dabei auch dem vorderen Teil der „Coiled-Coil“-Domaene eine wichtige Rolle zu. Im Gegensatz dazu war bei Mutanten mit Deletion des nicht-helikalen C-Terminus die PC-Bildung vollstaendig blockiert, was auf eine große Bedeutung dieser Domaene fuer die Polymerisation hinweist. Ein weiterer Hauptgegenstand der Arbeit war die Charakterisierung von Bindungspartnern von SYCP1. Über Immungoldlokalisation auf Maushoden konnten die Proteine Syce1 und Cesc1 als erste ausschliessliche Komponenten des CE des SC bestimmt werden. Zusaetzlich wurde die Interaktion dieser Proteine mit dem N-Terminus von SYCP1 verifiziert. SYCP1 bildet also die Grundstruktur des CE aus und rekrutiert Syce1 und Cesc1. / The synaptonemal complex protein 1 (SYCP1) is a structural component of the mammalian synaptonemal complex (SC), a meiosis-specific nuclear structure essential for synapsis, recombination and segregation of homologous chromosomes. The SC is a tripartite structure consisting of two lateral elements (LEs) and the central region (CR) with a central element (CE) in its middle. The LEs are attached to the axes of homologous chromosomes and are connected with the CE by transversal filaments (TFs). The protein SYCP1 (125 kDa) contains a long central á-helical domain, which is predicted to mediate dimerization in a parallel coiled-coil structure, flanked by two non-helical ends. The coiled-coil is thought to cross the gap between the LEs and the CE, the C-termini are anchored in the LEs and the N-termini have been localized to the CE. In order to better understand the molecular architecture of the SC and the role of SYCP1 in SC-assembly the polymerization properties of SYCP1 were investigated. To this end the protein was expressed in somatic cells. In this approach SYCP1 is able to form stable filamentous structures autonomously, which on the ultrastructural level represent alternating lines connected by TFs. This composition resembles multimeric SC-like complexes arranged in parallel, so called polycomplexes (PCs). By determining the orientation of SYCP1 molecules it was proven that PCs are highly ordered structures with the same arrangement of SYCP1 molecules as in SCs. These results demonstrate that SYCP1 is able to assemble into structures closely resembling the CR of SCs even in the absence of other SC-proteins, which signifies that SYCP1 is the primary determinant of SC assembly which in turn plays a key role in synapsis of homologous chromosomes. For a more detailed analysis, selected mutated constructs of SYCP1 were expressed. Mutations that modified the length of the central alpha-helical domain resulted in the formation of PCs consisting of repeat units of altered width, verifying that the coiled-coil domain determines the distance between the lines of the PC. This result implies the same function of this domain in SC assembly. Moreover, it was observed that SYCP1 molecules lacking the non-helical N-terminus are still able to form PCs, albeit at a strongly reduced level. This shows the importance of the N-terminus both in SYCP1 autoassembly and in the formation of the CE of SCs, but also implies a significant role of the N-terminal part of the coiled-coil domain. In contrast, when the non-helical C-terminus was deleted, filament formation was eliminated indicating a major role of the C-terminus in SYCP1 autoassembly. Another major topic of this work was the characterization of SYCP1 binding partners. By immunogold localization on mouse testis the proteins Syce1 and Cesc1 could be identified as the first exclusive components of the CE of SCs. Furthermore, the interaction of these proteins with the N-terminal region of SYCP1 was validated. Hence, SYCP1 forms the basic structure of the CE and recruits Syce1 and Cesc1.

No description available.

Fiber coils had been widely investigated as optical current sensors for a long time. In this thesis we have fabricated the LMA-10 PCF coils. By using the Sagnac fiber loop, we can obtain the transmission spectrum of the PCF coils. The measured birefringence of the SMF coil and the PCF coil are 1.49¡Ñ10^-5 at £f= 959.27 nm and 1.31¡Ñ10^-5 at £f = 1264.3 nm, respectively. The birefringence of the SMF coil agrees well with the theoretical result. The properties of PCF coils for variant fiber turns and cylinder sizes are discussed. As we increase the number of fiber turns, the fringe spacing becomes smaller due to the increasing phase difference. The birefringence of the PCF coil decreases with the increasing cylinder radius. Besides, we also measure the temperature sensitivities of the SMF coil and PCF coil to be 130 pm/oC and 64.55 pm/ oC, respectively. We have also demonstrated the sensing properties of the PCF coils. By introducing a displacement along the cylinder, the bending on the PCF coil can be induced. The measured bending sensitivity is -3.732 nm/m^-1. In addition, the water depth sensing properties are obtained by horizontally and vertically immersing the PCF coils into the water. As we put the PCF coil horizontally into the water, the shift of the measured spectra shows a exponential relation to the water depth. As for the vertically immersed PCF coil, the linear water depth sensitivity is -11.658 nm/cm. Finally, we propose the transverse displacement sensor based on the PCF coil. The measured sensitivity to transverse displacement can be as large as 903.9 nm/cm.

In the last decade, the progress of personal computer is growing very fast. The frequency of signals on mother boards applies between 33MHz and 1GHz. Due to the high operating frequency, the radiation from the microstrip lines on PCB should be noticed when designing the PCB¡¦s layout. The solution is using solenoidal and rogowski coils which have been investigated for many years. Coils, however, not only can be used in RF inductors, but also induce currents due to magnetic field through the cross section wound in turns. Therefore, coils may be used to induce magnetic field. Using this phenomenon, intensity of currents on DUT(Device Under Test) could be measured as well. In order to make more extensive use, optimization routines on the basis of the model were found. This thesis shows that magnetic field sensors on silicon and PCB can be studied in a better model, simulated by the software, Ansoft HFSS. Simple accurate expressions, an equivalent circuit model, and the transfer impedance are presented. As a result, induction of magnetic field on silicon is demonstrated in the area enclosed by turns, the parasitic capacitance or inductance, and conductor resistance, which is due to the skin effect in the high frequency. Hence, coils can induce large current because of big cross section. We can use it to measure the magnetic field and the currents radiated by the microstrip on PCB. In this thesis, I will show a novel PCB sensor which can measure 100MHz to 4 GHz signals and it has large output signal as well. The advantages of these coils are less disturbing DUT and easy to produce. It proves that we can use these magnetic field sensors to help solving the EMI problems.

This thesis examines a single-stage barrel-less electromagnetic induction launcher, developed from an arrangement used previously for very high speed, low projectile mass accelerators. The projectile is placed on top of the launch coil, rather than inside the coil, as is more usually the case in both single and multi-stage launchers of this type. The examination is undertaken both theoretically and experimentally, with extensive experimental results being used to test the validity of the theoretical techniques used. The theoretical examination of the launcher is based on two distinct mathematical models, with results provided by the two approaches being compared. The first model uses a 2D electromagnetic finite-element approach, and does not include projectile motion or thermal effects. It is used to establish whether an analysis technique which does not take into account the projectile dynamics is a valid design tool for a single-stage launcher. The second model employs a coupled-circuit approach to take into account both projectile motion and thermal effects, and is used to obtain an accurate estimate of the overall launcher performance. With the aid of the two models the effects of varying many different launcher parameters are examined, including the size and shape of the stator coil and the size, shape and composition of the projectile. The launcher investigation could be based on a number of criteria but here it is primarily based on two; the highest average velocity over a given distance and the shortest time required to travel a given distance from the initial stationary position.

Solar energy is an important form of renewable energy that can be used as an alternative to fossil fuels. It can be used to produce electricity or to provide heat. One particular application is using solar energy for a domestic hot water system. The purpose of this research is to improve the thermal performance of a solar domestic hot water (SDHW) system. Experimental research was conducted to study the thermal performance of a shell-and-3coil heat exchanger and a shell-and-4coil heat exchanger using either water or glycol as working fluids on the tube side. An experimental set-up simulating a SDHW system was designed and constructed. The set-up contained a 270 L storage tank, a shell-and-three coil heat exchanger or a shell-and-four coil heat exchanger, and electrical heaters to simulate the solar collector. At the inlets and outlets of the storage tank and the heat exchanger the temperatures, pressures, and flow rates were measured to determine the thermal performance. The results from the experiment tests were analyzed in terms of the overall heat transfer coefficient product (UA) and the pressure drop (?P) between the inlet and outlet of the heat exchanger. The UA value of the shell-and-4coil heat exchanger was higher than the UA value of the shell-and-3coil heat exchanger. For example, at a heat transfer rate of 2000 W for water, the UA values were 240 W/K and 270 W/K for the shell-and-3coil heat exchanger and the shell-and-4coil heat exchanger, respectively. With respect to glycol, at a heat transfer rate of 2000 W the UA values were 197 W/K and 215 W/K for shell-and-3coil, and shell-and-4coil heat exchanger, respectively. The degradation of the thermal performance of the shell-and-3coil was offset by benefits, such as reduction in mass, volume, labor cost and the final cost. A reasonable agreement between theoretical and experimental results in terms of the UA value was observed. The thermal performance of each coil in both heat exchangers was below that predicted by the relevant heat transfer correlations. A performance factor was calculated for each coil. For both glycol and water, and both heat exchangers, the performance factors for the inner most and outer most coils were 0.70 and 0.53, respectively. However, there is a slight difference in the performance factors of coils between the inner most and the outer most coils for the 3-coil and 4-coil heat exchangers. For these coils the performance factors varied from 0.55 to 0.67.

Non-muscle myosin II (NM-II) is present in every cell type and moves actin filaments to provide contraction within the cell. NM-II has a motor domain, a neck domain that binds two light chains, a long coiled-coil tail domain, and a carboxyl-terminal tailpiece. NM-II forms bipolar filaments by associating near the carboxyl-terminus of the tail. It has long been known that both the formation of bipolar filaments and enzymatic activity of the motor domain are regulated by phosphorylation of one of the neck-binding light chains, known as the regulatory light chain (RLC). This phosphorylation causes a large-scale conformational shift of both the motor domains and the tail domain. However, the mechanism of this regulation and the elements that mediate the autoinhibition remain unknown. We have taken a deletional approach to finding the elements necessary for autoinhibition and regulation of filament assembly. We have used salt- dependent pelleting assays, cell culture, and analytical ultracentrifugation to identify two small regions in the IQ motifs of the neck and the carboxyl-terminal tailpiece that are essential for autoinhibition. Another necessary element for autoinhibition is the fold the coiled coil of the tail back on itself by means of hinge domains. We used internal deletions, pelleting assays, and thermal stability assays to identify and characterize the flexible hinge domains within the coiled-coil tail of NM-II. These hinges consist of low-stability regions of coiled coil, and can be stiffened by introducing mutations that cause the sequence to mimic a more ideal coiled coil. By defining these essential elements of autoinhibition, this work paves the way for a mechanistic understanding of the complex regulation of NM-II in the cell. This dissertation contains unpublished co-authored material. / 2015-07-11

Balanced steady-state free precession (bSSFP) is a fast, SNR-efficient magnetic resonance (MR) imaging sequence suffering from dark banding artifacts due to its off-resonance dependence. These banding artifacts are difficult to mitigate at high field strengths and in the presence of metallic implants. Recent developments in parametric modelling of bSSFP have led to advances in banding removal and parameter estimation using multiple phase-cycled bSSFP. With increasing number of coils in receivers, more storage and processing is required. Coil combination is used to reduce dimensionality of these datasets which otherwise might be prohibitively large or computationally intractable for clinical applications. However, our recent work demonstrates that some combination methods are problematic in conjunction with elliptical phase-cycled bSSFP.This thesis will present a method for phase estimation of coil-combined multiple phase-cycled bSSFP to reduce storage and computational requirements for elliptical models. This method is general and works across many coil combination techniques popular in MR reconstruction including the geometric coil combine and adaptive coil combine algorithms. A viable phase estimate for the sum-of-squares is also demonstrated for computationally efficient dimension reduction. Simulations, phantom experiments, and in vivo MR imaging is performed to validate the proposed phase estimates.Compressed sensing (CS) is an increasingly important acquisition and reconstruction framework. CS MR allows for reconstruction of datasets sampled well-under the Nyquist rate and its application is natural in MR where images are often sparse under common linear transforms. An extension of this framework is the ordering problem for CS, first introduced in 2008. Although the assumption is made in CS that images are sparse in some specified transform domain, it might not be maximally sparse. For example, a signal ordered such that it is monotonic is maximally sparse in the finite differences domain. Knowledge of the correct ordering of an image's pixels can lead to much more sparse and powerful regularizers for the CS inverse problem. However, this problem has met with little interest due to the strong dependence on initial image estimates.This thesis will also present an algorithm for estimating the optimal order of a signal such that it is maximally sparse under an arbitrary linear transformation without relying on any prior image estimate. The algorithm is combinatoric in nature and feasible for small signals of interest such as T1 mapping time curves. Proof of concept simulations are performed that validate performance of the algorithm. Computationally feasible modifications for in vivo cardiac T1 mapping are also demonstrated.

No description available.