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Molecular modelling of peptide folding, misfolding and aggregation phenomenaTodorova, Nevena, Nevena.Todorova@rmit.edu.au January 2009 (has links)
In this thesis we present computer modelling studies that were implemented to investigate protein behavior in various environments causing their folding, unfolding and aggregation. Applications related to two important proteins - insulin and apolipoprotein C-II (ApoC-II) are presented. The use of atomistic simulation methodologies based on empirical force fields has enhanced our understanding of many physical processes governing protein structure and dynamics. However, the force fields used in classical modelling studies are often designed for a particular class of proteins and rely on continuous improvement and validation by comparison of simulations with experimental data. In Chapter 4 we present a comprehensive comparison of five popular force fields for simulation of insulin. The effect of each force field on the conformational evolution and structural properties of the protein is analysed in detail and compared with available experimental data. A fundamental phenomenon in nature is the ability of proteins to fold ab initio to their functional native conformation, also known as their biologically active state. Due to the heterogeneity and dimensionality of the systems involved, it is necessary to employ methodologies capable of accelerating rare events, specifically, configurational changes that involve the crossing of large free energy barriers. In Chapter 5, using the recently developed method BE-META we were able to identify the structural transitions and possible folding pathways of insulin. Another interesting phenomenon is the misfolding of proteins causing their aggregation, that may lead to formation of either amorphous compounds or structures of elongated-unbranched morphology known as amyloid fibrils. The deposition of amyloid fibrils in the human body may cause many debilitating diseases such as Alzheimer's and variant Creutzfeldt-Jakob diseases, thus making this field of research important and urgent. The human plasma protein apoC-II serves important roles in lipid transport, and it has been shown to form amyloid-like aggregates in solution. We have performed computational studies to investigate the effect of mutations, such as Met oxidation and the residue substitutions to hydrophobic Val and hydrophilic Gln, on dynamics of apoC-II(60-70) peptide. The conformation features relevant to the amyloidogenic propensities of the peptide were identified and presented in Chapter 6. The involvement of lipids at the various stages of development of amyloid diseases is becoming more evident in recent research efforts. In particular, micellar and sub-micellar concentrations have showed to have different effect on fibril growth and kinetics of native apoC-II and derived peptides. In Chapter 7 we investigated the influences of phospholipids at various concentrations on the structure of apoC-II(60-70) using MD and umbrella sampling methods. The molecular mechanisms of lipid effects on the peptide conformation and dynamics were identified. In Chapter 8 preliminary results on the structural stability of pre-formed oligomeric composites of apoC-II(60-70) peptide of different sizes and arrangements were also presented. The effects of mutation (oxidised Met, Met60Val and Met60Gln) on the most stable cluster was also investigated. To conclude, several ideas for continuation of research in the protein folding and aggregation field are discussed in the Future Work section of this thesis.
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Interactions of class A and class L amphipathic helical peptides with model membranes /Polozov, Ivan V. January 1997 (has links)
Thesis (Ph.D.) -- McMaster University, 1997 / Includes bibliographical references (leaves 151-167) Also available via World Wide Web.
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Membrane chaperones : protein folding in the ER membrane /Kota, Jhansi, January 2007 (has links)
Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007. / Härtill 3 uppsatser.
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Biocomputational studies on protein structures /Nordling, Erik, January 2002 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2002. / Härtill 7 uppsatser.
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Protein folding, stability and recognition /Duan, Jianxin, January 2004 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.
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Protein disulfide isomerase : function and mechanism in oxidative protein folding /Xiao, Ruoyu, January 2005 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2005. / Härtill 4 uppsatser.
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Regulation of Hsc70 by J domain co-chaperones and nucleotide exchange factorsTzankov, Stefan. January 1900 (has links)
Thesis (M.Sc.). / Written for the Dept. of Biochemistry. Title from title page of PDF (viewed 2008/07/30). Includes bibliographical references.
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Integrative analysis of small GTP binding proteins in Caenorhabditis elegans functional clustering and role in the endoplasmic reticulum stress signaling /Caruso, Marie-Elaine. January 1900 (has links)
Thesis (Ph.D.). / Written for the Dept. of Experimental Surgery. Title from title page of PDF (viewed 2008/01/12). Includes bibliographical references.
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Protein secondary structure prediction using amino acid regularitiesSenekal, Frederick Petrus. January 2008 (has links)
Thesis (M.Eng.(Computer Engineering))--University of Pretoria, 2008. / Summaries in Afrikaans and English. Includes bibliographical references (leaves 150-160).
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Deciphering the protein folding code : ab initio prediction of protein structure /Simons, Kim T. January 1998 (has links)
Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [117]-125).
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