This study demonstrates the presence of protein kinase C activity in both cytosolic and membrane fractions of bovine lens epithelial cells in culture. Protein kinase C activity is similar in normal and hyperglycemic cells. Furthermore, the ability of the enzyme to translocate from the cytosol to the membrane following phorbol ester treatment is unimpeded by hyperglycemic conditions. Moreover, protein kinase C activation had no effect on myoinositol uptake either in normal cells or in cells exposed to hyperglycemic conditions.

L’augmentation de la longévité et une meilleure prise en charge des maladies cardiovasculaires entraînent un accroissement de la fréquence des maladies liées au vieillissement cérébral, les accidents vasculaires cérébraux (AVC) et la démence étant les plus fréquents. Les marqueurs IRM de vieillissement cérébrovasculaire (hypersignaux de la substance blanche [HSB], infarctus silencieux, microhémorragies) sont de forts prédicteurs d’AVC et de démence, très fréquents en population générale âgée et facilement mesurables. Nous avons étudié l’association entre des composantes du métabolisme lipidique (taux de lipides plasmatiques, génotype ε de l’Apolipoprotéine E [APOE]) et les marqueurs IRM de vieillissement cérébrovasculaire. Nous rapportons dans une revue systématique et méta-analyse que l’allèle APOEε4 est associé à un volume accru de HSB et à un risque accru de microhémorragies et que l’allèle APOEε2 est associé avec un volume accru de HSB et une fréquence plus élevée d’infarctus silencieux. Nous rapportons dans les études 3C-Dijon et EVA, que les taux croissants de triglycérides (TG) sont associés à un volume accru de HSB et à une fréquence plus élevée de lacunes (petits infarctus silencieux). Enfin nous avons exploré la signification clinique de ces associations dans l’étude 3C. Nous rapportons que des taux plus élevés de TG, LDL-cholestérol, et cholestérol total sont associés à un risque accru de démence et de ses sous-types, en population générale âgée de 74 ans à l’inclusion et suivie pendant 12 ans. Nous concluons que le métabolisme lipidique est associé aux marqueurs IRM de vieillissement cérébrovasculaire et à la démence. / Increasing longevity and improved management of cardiovascular diseases has led to an increase in the frequency of age-related neurological diseases, especially stroke and dementia. MRI markers of vascular brain injury (white matter hyperintensities [WMH], silent infarcts and microbleeds) are powerful predictors of stroke and dementia, very frequent in the elderly, and can be measured easily. We studied the association between some components of lipid metabolism (plasma lipid levels, Apolipoprotein E [APOE] ε genotype) and MRI markers of vascular brain injury. We found in a systematic review with meta-analysis that the ε4 allele of the APOE gene is associated with larger WMH volume and a higher frequency of cerebral microbleeds, and that the APOEε2 allele is associated with larger WMH volume and a higher frequency of silent brain infarcts. We also report in the 3C-Dijon Study and in the EVA study that higher triglyceride levels are associated with an increased WMH volume and with a higher frequency of silent lacunar (small subcortical) brain infarcts. Finally, we investigated the clinical significance of these associations the 3C Study. We observed that higher triglycerides, LDL-cholesterol and total cholesterol levels, were associated with an increased risk of all dementia and its subtypes, in community persons aged 74 years at baseline and followed for up to 12 years. We conclude that lipid metabolism is associated with MRI-markers of cerebrovascular aging and dementia.

The aim of this study is to investigate how the connection between fractions, decimals and percent are taught in grade 4-6 with more focuson the fractions. The empirical data was obtained by qualitative methods comprising interviews with four mathematic elementary school teachers, in addition to two observations with two classrooms in grade 6. The data presented is from one school. The theoretical framework is based on Liping Ma profound understanding of fundamental mathematics and theories of subject didactic concepts of Kilborn, Löwing, Karlsson & Kilborn and MacIntosh. The results of the interviews and observations show that the connection between fractions, decimals and percent is being taught without illuminating how the mentioned are connected. The aspect of fractions, which has been taught to show the relation between fractions and decimals, was division as metaphor. While there was no aspect of fractions has been taught to show the relation between it and percent except that a percent is a hundredth. Such as 40% is equal with 40/100. In addition, fractions has been taught by using visual aids, but never taught by using number line. In conclusion the connection between fractions, decimals and percent has not been related clearly with basic concept fractions.

La fin des activités industrielles liées au charbon et à son exploitation a laissé de nombreux sites et sols pollués en France et particulièrement dans le Nord-est de la France. Une terre issue d'une friche industrielle de cokerie (Neuves-Maisons, Lorraine, France) est étudiée en couplant les outils de la science du sol et de la géochimie organique afin de comprendre l'évolution à long terme de la dynamique des contaminants. Une première étape de caractérisation de la terre et de sa pollution montre que la fraction limon fin (2-20 µm) est la fraction la plus réactive avec la plus forte concentration en HAP extractible et occlus et la plus faible disponibilité, mettant en évidence des processus de préservation spécifique au sein de cette fraction. Des extractions sélectives de la MO anthropique au solvant, confirmées par des observations au MEB, montrent que la MO anthropique, contrairement à la MO naturelle, n'a pas un rôle de liant entre les particules de la terre. L'apport de MO naturelle, (i) en conditions contrôlées par incubation de débris végétaux pendant 15 mois ou (ii) in situ en parcelles lysimétriques plantées avec de la luzerne pendant 6 ans, conduit à une structuration de la terre, une activité microbienne accrue et à l'incorporation et au stockage de la matière organique fraîche végétale dans les limons fins. En revanche, la pollution n'est pas affectée par l'apport de matière organique fraîche. Ainsi, la concentration et la disponibilité des HAP présentent une tendance à la diminution au cours du temps, que la terre soit cultivée ou non. Ainsi, la matière organique fraîche n'apparaît pas comme un agent de mobilisation de la pollution à long terme pour ce type de terre historiquement contaminée / The end of industrial activities associated to coal exploitation left many huge areas of wastelands especially in the Northeast of France. A former coking plant soil was studied with a combination of soil science and organic geochemistry tools in order to understand the evolution of the contaminants dynamic. In a first step, the soil and its pollution were characterized. The results showed that the fine silt fraction (2-20 µm) was the most reactive with the highest PAH concentration (extractable or occluded) and the lowest PAH availability. This fraction played a protective role toward the pollution. Moreover, solvent extractions of the anthropogenic organic matter showed that the anthropogenic organic matter was not a structuring agent between the particles in the soil. These observations were confirmed by SEM observations. In a vegetation growth context, (i) under controlled conditions with incubation of organic residue during 15 months and (ii) in situ in lysimetric plots planted with alfalfa during 6 years, the soil structure and the microbial biomass were promoted and the fresh organic matter was preferentially incorporated and stored in the fine silts. However, the pollution was not affected by the fresh organic matter addition. Both PAH concentration and availability showed a trend to decrease with the time of experiment. Thus, the fresh organic matter did not appear as a mobilizing agent for the pollution and would most probably contribute to pollution stabilization on the long term

La tuberculose est une maladie d'urgence dans le monde, l'apparition de souches résistantes au traitement a produit l'utilisation de produits naturels comme alternative. Des études ont montré que les extraits de Leucophyllum frutescens présentaient un effet antimicrobien, mais l'inconvénient est que les extraits sont récupérés dans un véhicule qui contient des solvants organiques. La préparation de nanoparticules polymériques (NP) implique l'élimination du solvant dans lequel l'actif est solubilisé, ce qui permet les utiliser comme véhicules pour l'administration des extraits. Par conséquent, le but de cette étude a été design et développer des formulations de NP avec un extrait de L. frutescens et de la rifampicine (RIF), afin d'évaluer l'activité biologique in vitro contre M. tuberculosis. Premièrement, l'extrait méthanolique de feuilles et de racines de L. frutescens et ses fractions a été obtenu. L'activité contre M. tuberculosis a été déterminée, l'extrait de racines (EMR) et ses fractions hexanique (FHR et RF1) ont été les plus actives avec une CMI de 100, 40 et 40 μg/mL, respectivement. RIF, EMH, FHR et RF1 ont été incorporés dans NP par nanoprécipitation. Des NP de ≈180 nm avec une distribution de taille homogène ont été obtenus. Les NP ont été évaluées sur M. tuberculosis, les formulations de NP-PLGA-RIF (CMI=0,10 μg/mL) et NP-PLGA-RF1 (CMI=80 μg/mL) ont montré la meilleure activité. Finalement, l'activité des formulations combinées contre M. tuberculosis a été évaluée, la combinaison de RIF avec NP-PLGA-RF1 a produit le meilleur comportement, réduisant la CMI des deux sans montrer un effet toxique. Les études réalisées dans ce travail ont montré l'utilisation potentielle d'une formulation de NP contient une fraction végétale de L. frutescens en combinaison avec le RIF comme alternative contre M. tuberculosis / Tuberculosis is an emergency disease worldwide, the emergence of resistant strains to the treatment has produced the use of natural products as alternative. Studies have shown that Leucophyllum frutescens extracts present antimicrobial effect, but the disadvantage is that the extracts are recovered in a vehicle that contains organic solvents. The preparation of polymeric nanoparticles (NP) involves the elimination of the solvent in which the active is solubilized, which makes possible to use them as vehicles for the administration of the extracts. Therefore, the aim of this study was to design and develop formulations of NP with an extract of L. frutescens, and rifampicin (RIF), in order to evaluate the in vitro biological activity against M. tuberculosis. Firstly, the methanolic extract of leaves and roots of L. frutescens and its fractions were obtained. The anti-M. tuberculosis activity was determined, being the root extract (EMR) and its hexane fractions (FHR and RF1) the most actives with a MIC of 100, 40 and 40 μg/mL, respectively. RIF, EMH, FHR and RF1 were incorporated into NP by nanoprecipitation. NP of ≈180 nm with homogeneous size distribution were obtained. The NP were evaluated on M. tuberculosis, being the formulation of NP-PLGA-RIF (MIC=0.10 μg/mL) and NP-PLGA-RF1 (CMI=80 μg/mL) with better activity. Finally, the anti-M. tuberculosis activity of the combined form formulations was evaluated, the combination of RIF with NP-PLGA-RF1 produced better behavior, reducing the MIC of both without showing toxic effect. The studies carried out in this work showed the potential use of an NP formulation contains a vegetable fraction of L. frutescens in combination with RIF as an alternative against M. tuberculosis

Submitted by Filipe dos Santos (fsantos@pucsp.br) on 2018-07-18T11:45:25Z No. of bitstreams: 1 Monique Lopes dos Santos Dias.pdf: 1428885 bytes, checksum: 6c52690071d28ac219840c2927010f6a (MD5) / Made available in DSpace on 2018-07-18T11:45:25Z (GMT). No. of bitstreams: 1 Monique Lopes dos Santos Dias.pdf: 1428885 bytes, checksum: 6c52690071d28ac219840c2927010f6a (MD5) Previous issue date: 2018-03-12 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This study aims to present the mapping of dissertations and theses produced by universities in the state of São Paulo between 2000 and 2016, related to the issue of fractional numbers. For the constitution of our corpus of research, we have selected 39 academic papers produced by the following universities: Pontifícia Universidade Católica de São Paulo (PUC-SP); Paulista State University "Júlio de Mesquita Filho" (UNESP); Federal University of São Carlos (UFSCar); Federal University of ABC (UFABC); State University of Campinas (Unicamp); Universidade Bandeirante de São Paulo (Anhanguera) and Cruzeiro do Sul University (UNICSUL). In the search for answers to the following research question "What are the main contributions of researches carried out by universities in the state of São Paulo, between the years 2000 and 2016, regarding the fractional numbers? What possible gaps and challenges are still presented on the subject?", we split the papers that compose our corpus into three distinct categories of analysis: approach of fractional numbers in official documents or in didactic materials; teacher’s training, initial training and continuing education; and teaching and learning of fractional numbers and their operations. As a result, we point out advances in the approach of fractional numbers through their different conceptions (part-whole, measure, quotient, operator and ratio), in all aspects observed in our mapping. Concerning the gaps and challenges in this field of study, we stress the exaggerated emphasis on the part-whole conception, albeit with all the criticisms imbued with it, besides the insufficiency of studies related to High School, Higher, Youth, Adult and Inclusive Education. We suggest that new research fill these gaps in order to broaden the contributions in the field of Mathematics Education / Este estudo tem o objetivo de apresentar o mapeamento de dissertações e teses produzidas por universidades do estado de São Paulo, entre os anos de 2000 e 2016, em relação ao tema números fracionários. Para a constituição de nosso corpus de pesquisa foram selecionados 39 trabalhos acadêmicos, produzidos pelas respectivas universidades: Pontifícia Universidade Católica de São Paulo (PUC-SP); Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP); Universidade Federal de São Carlos (UFSCar); Universidade Federal do ABC (UFABC); Universidade Estadual de Campinas (Unicamp); Universidade Bandeirante de São Paulo (Anhanguera) e Universidade Cruzeiro do Sul (UNICSUL). Na busca de respostas para a seguinte questão de pesquisa “Quais as principais contribuições das pesquisas realizadas por universidades do estado de São Paulo, entre os anos de 2000 e 2016, no que diz respeito aos números fracionários? Que possíveis lacunas e desafios ainda são apresentados quanto ao tema?”, dividimos os trabalhos que compõem nosso corpus em três categorias distintas de análise: abordagem dos números fracionários em documentos oficiais ou em materiais didáticos; saberes docentes, formação inicial e formação continuada; e ensino e aprendizagem dos números fracionários e de suas operações. Como resultados, apontamos avanços quanto à abordagem dos números fracionários por meio de suas diferentes concepções (parte-todo, medida, quociente, operador e razão), em todos os aspectos observados em nosso mapeamento. Concernentes às lacunas e desafios nesse campo de estudo, destacamos a ênfase exagerada à concepção parte-todo, ainda que com todas as críticas imbuídas a ela, além da insuficiência de estudos correlatos ao Ensino Médio, ao Ensino Superior, à Educação de Jovens e Adultos e à Educação Inclusiva. Sugerimos que novas pesquisas sanem essas lacunas, a fim de ampliar as contribuições no âmbito da Educação Matemática

Les pétroles utilisés en raffinage étant de plus en plus lourds et chargés en métaux et hétéroélements, il est d’une grande importance pour les activités de raffinage de connaître la spéciation de ces espèces au sein des produits pétroliers et leurs comportements durant les procédés de raffinage. Afin d’apporter des réponses à cette problématique des techniques de caractérisation élémentaire et moléculaire ont été utilisées notamment par ICP MS Haute Résolution et FT ICR MS respectivement. Ces techniques analytiques sont appliquées à différents échantillons pétroliers provenant parfois de procédés de raffinage et les informations obtenues au niveau élémentaire et moléculaire sont utilisées de manières complémentaires pour améliorer notre compréhension des mécanismes se produisant au sein de nos échantillons durant les activités de raffinage. / The crude oils available for the refining industry are heavier and heavier and more concentrated in metals and heteroelements. It is thus of great importance to study the speciation of these species within the petroleum products and their behavior during the refining processes. To answer that problematic, elemental and molecular characterization techniques have been used, mainly High Resolution ICP MS and FT ICR MS for the elemental and molecular characterization respectively. The analytical techniques quoted are used for the analysis of several petroleum products sometimes originating from refining processes and the information obtained at the elemental and molecular level are combined to improve our understanding of the mechanisms occurring within our samples during refining activities.

Submitted by Rosivalda Pereira (mrs.pereira@ufma.br) on 2017-06-21T17:13:47Z No. of bitstreams: 1 AndreiaAmorim.pdf: 2021730 bytes, checksum: d4f2de640b2254f58c621285962a6333 (MD5) / Made available in DSpace on 2017-06-21T17:13:47Z (GMT). No. of bitstreams: 1 AndreiaAmorim.pdf: 2021730 bytes, checksum: d4f2de640b2254f58c621285962a6333 (MD5) Previous issue date: 2016-06-27 / Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA) / The use of chemical indicators used to evaluate soil quality in a growing alley cropping can identify the causes of land degradation in agricultural systems of the Amazon, the conversion of forest to these agricultural systems, incompatible with soil and climatic conditions of this region. This degradation is considered one of the greatest threats to the rainforest due to depletion of phosphorus (P), the decline in soil organic matter (SOM) and the loss of basic cations. two kinds of high quality waste were used: (Leucaena leucocephala and Gliricidia sepium), and two species of low quality waste (Clitoria fairchildiana and Acacia mangium). The 1600m2 area was sampled for carrying out chemical analysis and fractionation of organic matter and soil phosphorus. The SOM was separated by density fractionation soil. Fractionation of P in soil was carried out with extraction solutions. The experimental design was randomized blocks: leucena + sombrero (L + S); leucena + acacia (L + A); gliricidia + acacia (G + A); gliricidia + sombrero (G + S) and control treatment without pulses with five treatments and eight repetitions. The corn was used as test culture. Legumes were pruned to 50 cm of soil waste materials were added to the surface and then the corn was sown. All soil samples of the experiment in alleys had low pH values. The results show statistical differences between treatments when to soil organic matter (SOM). The highest levels were found in the treatment leucena + sombrero (L + S), but did not influence the carbon stock of Fractions Take Free (FLL), Intra-aggregate fractions (FLI) and MOP MOS, which showed no statistical difference. Therefore, in accordance cutting alleys, most of the P found in inorganic form, and was also found in fractions moderately labile and difficult. The results indicate that the fractions of P and organic matter are important indicators to evaluate changes in the degree of land degradation in the humid tropics. These results also indicate that the intensive and continuous use of annual crops in soils of the regions of the Amazon without any conservation practice can be considered as a high risk to the sustainability of agricultural systems, mainly because of increased active and potential acidity, reducing labile organic matter and depletion of organic P pools, leading to soil degradation. Soil fertility, physical properties and available P are responsible for the variability of soil degradation. Considering that small farmers are responsible for 70% of agricultural production in the country, the sustainable management of these soils, usually of low fertility by agroecological alley cropping of legumes is an alternative to agriculture as socioeconomic and environmental benefits. / O uso de indicadores químicos serve para avaliar a qualidade do solo em um sistema de cultivo em aleias. A degradação do solo de sistemas agrícolas da Amazônia, pela conversão da floresta para esses sistemas agrícolas, incompatíveis com as condições edafoclimáticas desta região é considerada uma das maiores ameaças para os solos tropicais devido à depleção de fósforo (P), a diminuição da matéria orgânica do solo (MOS) e a perda de cátions básicos. Esta Tese teve o objetivo de avaliar os principais indicadores químicos de qualidade do solo (matéria orgânica e seus compartimentos, pH, frações do fósforo (P), potássio (K) e o somatório de cálcio (Ca) e magnésio trocáveis (Mg)) após dois anos de plantio em um ARGISSOLO VERMELHO AMARELO Distrocoeso sobre um sistema de cultivo em aleias. Foram utilizadas duas espécies de alta qualidade de resíduos: (Leucaena leucocephala e Gliricídia sepium), e duas espécies de baixa qualidade de resíduos (Clitoria fairchildiana e Acacia mangium). A área de 1600m2 foi amostrada, para realização das análises químicas (IAC, 2001) e fracionamento densimétrico e granulométrico da MOS (MACHADO, 2002). O fracionamento de P no solo foi realizado com soluções extratoras, conforme Hedley et al. (1982) com as modificações de Condron et al. (1985), adaptado de Gatiboni (2003). O delineamento experimental foi em blocos casualisados com cinco tratamentos e oito repetições: leucena+sombreiro (L+S); leucena+acácia (L+A); gliricídia+acácia (G+A); gliricídia+sombreiro (G+S) e tratamento controle sem leguminosas. O milho foi utilizado como cultura teste. As leguminosas foram podadas a 50 cm do solo e seus resíduos foram adicionados à superfície e em seguida o milho foi semeado. Todas as amostras do solo do experimento em aleias apresentaram valores baixos de pH. Houve diferenças estatísticas entre os tratamentos para a MOS. Os maiores teores foram encontrados no tratamento leucena+sombreiro (L+S), mas não influenciou nos estoque de carbono das Frações Leve Livre (FLL), Frações Intra-agregadas (FLI) e Matéria Orgânica Particulada (MOP) da MOS, que não apresentaram diferença estatística. Por conseguinte, após o corte das aleias, a maior parte do P foi encontrada numa forma inorgânica, e também foi encontrada nas frações moderadamente e dificilmente lábeis. Os resultados indicam que as frações de P e matéria orgânica são importantes indicadores para avaliar mudanças no grau de degradação da terra nos trópicos úmidos. Estes resultados indicam também que o uso intensivo e contínuo das culturas anuais nos solos das regiões da Amazônia sem nenhuma prática conservacionista pode ser considerado como um risco elevado para a sustentabilidade dos agrossistemas, principalmente por causa do aumento da acidez ativa e potencial, a redução da matéria orgânica lábil e o esgotamento dos pools de P orgânico, levando à degradação do solo. A fertilidade do solo, propriedades físicas e P disponível são responsáveis pela variabilidade da degradação do solo. Considerando-se que os pequenos agricultores respondem por 70% da produção agrícola no país, a adoção do manejo sustentável dos solos cultivados, geralmente de baixa fertilidade natural com sistema em aleias de leguminosas é uma alternativa para a agricultura com benefícios socioeconômicos e ambientais.

No description available.

Le blé est la seconde céréale la plus cultivée dans le monde et constitue un apport majeur de l’alimentation quotidienne. L’effort consenti à continuellement améliorer les qualités meunière et boulangère du blé tendre, s’est fait au détriment du caractère nutritionnel du grain. Ainsi la plupart des produits industriels dérivés des grains de blé sont produits à partir de farines blanches raffinées qui ne contiennent ni le germe ni les sons. Cependant, dans ces différents tissus qui sont éliminés et qui servent essentiellement à nourrir les animaux, se concentrent les principaux micronutriments tels que les vitamines, les minéraux, les fibres et des substances phytochimiques. Les différentes enquêtes épidémiologiques ont bien mis en évidence les conséquences négatives de la déplétion en micronutriments des produits céréaliers raffinés. Dans l’objectif d’une alimentation plus saine voir même préventive, la consommation d’aliments enrichis en micronutriments naturellement présents dans le grain de blé tendre semble être une démarche efficace. Dans cette optique, ce travail de thèse a permis de consolider et d’accroitre les connaissances concernant les voies d’amélioration des teneurs en vitamines des grains de blés tendres ainsi que des produits industriels qui en sont dérivés. En premier, nous nous sommes intéressés au développement d’une méthode simple et rapide basée sur la spectrométrie de masse couplée à la chromatographie liquide pour la détermination simultanée de sept vitamines hydrosolubles dans divers matériels végétaux. Les vitamines présentes dans les différents matériels végétaux furent séparées en moins de 15 min grâce à l’utilisation d’une colonne C18 en phase inverse, et analysées en mode ElectroSpray positif et MRM. La réponse pour toutes les vitamines a été linéaire sur l’ensemble des concentrations étudiées (0.05 to 9 μg/mL) avec des coefficients de corrélation compris entre 0.991 et 1. Les limites de quantification de la méthode analytique ont été évaluées entre 0.09 et 3.5 μg/g. Les précisions intra-journalière et inter-journalière étaient satisfaisantes. La deuxième partie de nos travaux a concerné l’impact des procédés de transformation du grain (production d’une nouvelle fraction de mouture et grillage) sur la teneur en vitamines. Afin de réaliser cette objectif, la méthode développée a été appliquée pour l’analyse simultanée des concentrations en vitamines hydrosolubles contenues dans différentes farines semi-complètes ainsi que dans les pâtons, pains et pains grillés qui en sont dérivés. En parallèle, les concentrations endogènes des vitamines E, de la Lutéine et du β-sitostérol ont également été évaluées dans le même matériel. Nous avons mis en évidence que les concentrations en acide nicotinique, pyridoxale, pyridoxine et acide pantothénique étaient significativement plus élevées dans les gros sons que dans les autres fractions de moutures, alors que les concentrations en β-sitostérol, lutéine, α-tocotriénol, α-tocophérol et thiamine (20.87 μg/g DM)étaient plus importantes dans la fraction de mouture enrichie. L’étape de grillage induit une augmentation significative en α-tocophérol (+216%), β-γ-tocophérol (+52%), α-tocotriénol (+83%), β-γ-tocotriénol (+32%), acide nicotinique (+55%), nicotinamide (+97%) et en pyridoxine (+77%). L’ensemble de ces résultats nous a permis de montrer qu’un enrichissement de farine blanche par la fraction de mouture dite enrichie pourrait potentiellement permettre d’accroître les produits qui en dérive en vitamine E. De plus le grillage pourrait libérer des composés bioactifs, augmentant ainsi leur biodisponibilité et la valeur nutritionnelle des pains. (...) / Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. During the course of improving the baking quality of wheat cultivar, most of the nutritional attributes have been underestimated. It is therefore unfortunate that most of wheat-based food products are mostly produced from refined white flour from which peripheral tissues (germ and envelopes) are removed. However, these tissues, which are eliminated and serve mainly for animal feeding, contain most of the vitamins, minerals, fiber and phytochemicals of the grain. It is becoming evident that many of the health benefits associated with the consumption of whole grain cereal products, relate to the enhanced intake of micronutrients, phytochemicals and dietary fiber. In the context of consuming wheat derived foods with enhanced nutritional value, as part of a healthy diet, this thesis provide results which strengthen the knowledge of vitamins accumulation in common wheat and in wheat-based food products. Firstly, we have developed a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous screening of seven water soluble vitamins in various wheat-based food materials. The vitamins present in the test materials were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision was found satisfactory. The second part of our research, have focused on monitoring the levels of vitamins upon the wheat-based foods processing operations, such as production of new wheat milling fraction (consisting in enriched fraction) and breadmaking toasted bread. In order to achieve this goal, the developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products. In addition the vitamin E, Lutein and β-sitosterol natural content was also measured in the same materials. It was shown that the concentration of nicotinic acid, pyridoxal, pyridoxine, pantothenic acid were significantly higher in the coarse bran than in the other milling fractions, while the concentration of β-sitosterol, lutein, α-tocotrienol, α-tocopherol and thiamin (20.87 μg/g DM) were the highest in the enriched fraction. The toasting step induced a significant increased of α-tocopherol (+216%), β-γ-tocopherol (+52%), α-tocotrienol (+83%), β-γ-tocotrienol (+32%), nicotinic acid (+55%), nicotinamide (+97%) and of pyridoxine (+77%). Furthermore, it was demonstrated that the enriched fraction could be a functional ingredient in order to enrich wheat-based products in fat soluble vitamins and that the toasting process could release bound bioactive compounds and led to enhance the nutritional quality of bread. (...)