工業革命後傳統經濟發展模式成功地使經濟活動與經濟成長成為世界上大多數國家的政治議題，但大量提高產業生產的結果使得地球上的資源消耗與廢棄物製造量所造成的生態耗損速度遠超過自然再生速度。另一方面，由於「科技」與「貿易」的影響，生物新陳代謝加諸於人類的負荷將透過產業的新陳代謝更形擴大，在高度工業化國家物質消費程度與形式更受到消費主義的鼓舞，形成毫無節制的消費與浪費。近年來台灣地區加入世界貿易組織（World Trade Organization， WTO）引起廣泛的環境與貿易議題的討論，其中農業議題尤為顯著，究竟台灣地區加入WTO後之農地轉用政策是否可使土地資源利用達到永續經營之目標，引發本研究之研究動機。 本研究以生態足跡分析及貿易與永續發展相關理念為基礎，以1991到1996年台灣地區的農業產業做為研究的時空範圍，進行台灣地區耕地與牧草地生態足跡之實證計算並加以分析。在理論方面，首先就永續性之整體面向及各別領域面向推論出生態經濟學之定義符合整體面向之強永續性觀念；其次重新思考人類容受力定義，非以支持最大人口數（單位：人╱土地）而是轉向計算某一族群在持續基礎下生產所有消費物質及消化所有廢棄物所需的土地及水域面積（單位：土地╱人）之生態足跡（ecological footprint）觀念；在貿易與永續發展理念推論部份，則以IISD（1994、1995）、ICTSD（1995）及Cosbey（1998）等之相關研究作為理論基礎；最後，結合生態足跡、貿易及永續發展三者之理論。 在實證方面，本研究修正國內外多項研究後，重新定義符合台灣地區之生態足跡計算項目，並計算台灣地區1991到1996年農業（耕地與牧草地）之生態足跡，得知台灣地區農業消費中佔耕地生態足跡比例較重的項目分別為稻米、其他作物、豆類、種子油及進出口紗布、紡織製成品、成衣及服飾品及鞋類等五項，而影響台灣地區農業之牧草地生態足跡面積較大的因子包括羊毛及皮革、獸皮兩項；將這些項目與行政院農委會WTO農業因應對策項目對照後，發現影響最大的兩項分別為稻米及農園特產之豆類；因此本研究就稻米與豆類兩項產業加入WTO前後對台灣地區生態足跡、生態標竿、及生態赤字之影響進行進一步研究，並分析該兩項產業完全轉用為非農業使用與完全無轉用對生態足跡之影響，以評估該兩項產業加入WTO是否為一永續性策略。 本研究的研究結果發現，不論是稻米或豆類，其生態足跡與耕地生態標竿間的差距在無論有無造成農地轉用的情形下，都對該值影響不大，亦即不因加入WTO而有所改變。就生態足跡的理念觀之，若加入WTO使進口量增加的結果造成產業生產力提昇、進口產業以內銷為主、對國內生產量影響程度愈大，相對生態足跡面積降低程度愈大者及農地轉用為同類別使用等這四種影響的情形之下，則台灣地區加入WTO可視為永續性策略。 / After the Industrial Revolution, traditional economic development models have successfully made economic activities and economic growth a political issue for most countries around the world. However, with the greatly enhanced industrial productivity has come instensified uses of the resources of the earth, and the ecological depletion rate resulting from solid waste volumes has far exceeded nature's regenerative capacities. On the other hand, due to the influence of technology and trade, the natural biological metabolism, already carrying a human load, is being expanded and intensified by the industrial metabolism. In highly industrialized countries, the degree and form of material consumption has been inspired by consumerism, engendering virtually unlimited consumption and waste. In recent years, while Taiwan has been seeking to join the World Trade Organization (WTO), there have been pervasive discussions on the subject of trade and the environment. Among these, agricultural issues have been prevalent. Should Taiwan join the WTO, will the farmland transformation policy permit land resource usage to meet the objectives for sustainable development? This question is the motivation for this dissertation. This dissertation is based on the ecological footprint analysis, along with trade and sustainable development theories. The timeframe for reseach extends from 1991 to 1996, focussing on Taiwan's agricultural industry. Ecological footprint analyses were calculated based on factual evidence/data from Taiwan's arable land and pasture land. In terms of applied theories, first, considering both the comprehensive entirety as well as individual discrete segments, we conclude that the definitions of eco-economics are in agreement with the concept of the strong sustainability. Next, we reconsider the definition of human carrying capacity, not basing this concept on the support of a maximum population base (Unit: per capita/ha), but rather on the calculation of the land and water needed by a certain group (Unit: ha/per capita), existing on a continuous base, to effectively produce all consumer materials and absorb all solid waste. In respect to trade and sustainable development theories, we use related studies such as IISD（1994、1995）, ICTSD（1995）, and Cosbey（1998） to be our theoretical base. Finally, we combine the three theoretical constructs, namely the concept of the ecological footprint, trade, and sustainable development. In carrying out our research on factual evidence/data, this dissertation modifies research previously conducted by a number of researchers. We redefine the calculation parameters for Taiwan's ecological footprint, finely calculating the ecological footprint for the agriculture industry (arable land and pasture land) for Taiwan in the 1991 to 1996 period. We learn that in terms of agricultural usage and consumption in Taiwan, the five key items with the largest shares of the arable land footprint are: rice, other cereals, pulses, seed oils, and import/export textile yarn, fabrics, articles of apparel and clothing accessories, footwear. Factors demonstrating the greatest influence on Taiwan's pasture land ecological footprint are: wool and leather, and animal hides. When we compare these findings with policies of the Council of Agriculture for entering into the WTO, we confirm that rice and pulses are indeed the two items of greatest importance. Therefore, this dissertation will focus on the rice and pulses industry before and after entering into the WTO, and on their influence on Taiwan's ecological footprint, ecological benchmark, and ecological deficit. We will also analyze possible consequences should these two industries be transformed to non-agricultural uses, or, if no such changes are instituted, what the consequences will be for Taiwan's ecological footprint. Based on these findings, we will evaluate whether the allowance of entry of these two industries into the WTO is a sustainable strategy. Our research findings reveal that, whether or not we transform farmlands from the rice and pulses industries, there will be no significant impacts on the anticipated gap between the ecological footprint and the arable land ecological benchmark. This means there will be no significant change should there be entering into the WTO. Thus, from the perspective of ecological footprint analysis, if we join the WTO and the result is an increase in imports, this will enhance our industrial productivity; imported items will be fully consumed domestically; the greater the influence on domestic production volumes the greater the decrease in the ecological footprint area; and, farmlands will be transformed to non-agricultural uses. Because of the above four factors, it can be said that joining the WTO will probably be considered as a policy of sustainability. 第一章 緒論 1 第一節 研究背景 1 第二節 研究目的與內容 5 第三節 研究流程與問題限制 9 第二章 基本理念與文獻評析 13 第一節 永續性理念及知識性工具 13 第二節 永續發展評估工具－生態足跡分析 26 第三節 貿易與永續發展關係之研究 44 第四節 生態足跡的比較利益基礎 57 第五節 永續發展與WTO環境與貿易議題之探討 63 第三章 永續發展與WTO相關農業議題探討 69 第一節 台灣地區加入WTO各項農業議題之協定 69 第二節 台灣地區加入WTO各項農業議題之對策 84 第三節 台灣地區加入WTO農業議題對永續發展之影響 94 第四章 台灣地區農業生態足跡分析實證研究 99 第一節 生態足跡分析架構之建立 99 第二節 台灣地區歷年農業生態足跡評估 109 第三節 台灣地區農業生態標竿之建立與生態足跡分析結果 118 第五章 台灣地區稻米及農園特產之生態足跡與WTO農業相關議題之探討.124 第一節 台灣地區農業生態足跡與WTO農業議題對應項目分析 124 第二節 台灣地區因應加入WTO農業議題對應項目與台灣地區稻米及農園特產生態足跡影響因子分析 130 第六章 加入WTO對台灣地區永續發展影響之探討 142 第一節 以生態足跡觀點解讀加入WTO是否為永續性策略 142 第二節 以貿易與永續發展原則檢視台灣地區加入WTO是否為永續性策略 146 第七章 結論與建議 149 第一節 結論 149 第二節 後續研究建議 154

Interest in accounting for environmental impacts of products, processes, and systems during the design phase is increasing. Numerous studies have undertaken investigations for reducing environmental impacts across the product life cycle. Efforts have also been launched to quantify such impacts more accurately. Life cycle energy consumption and carbon footprint are among the most frequently adopted and investigated environmental performance metrics. As efforts continue to incorporate environmental sustainability into product design, struggles persist in concurrent consideration of environmental impacts resulting from the manufacturing processes and supply chain network design. Thus, the objective of this research is to present a framework for reducing product cradle-to-gate energy consumption and carbon footprint through simultaneous consideration of manufacturing processes and supply chain activities. The framework developed in this thesis relies on unit process modeling, and is demonstrated for production of a bicycle pedal. It is shown that simultaneous consideration of manufacturing and supply chain processes can impact decision-making and improve product environmental sustainability at the design stage. The work presented contributes to the state of the science in sustainable design and manufacturing research. In addition, a point of departure is established for the research community to move current efforts forward for concurrent consideration of multiple stages of the product life cycle in pursuit of environmental, economic, and social sustainability. / Graduation date: 2012

Modern food production is a complex, globalized system in which what we eat and how it is produced are increasingly disconnected. This thesis examines some of the ways in which global trade has changed the mix of inputs to food and feed, and how this affects food security and our perceptions of sustainability. One useful indicator of the ecological impact of trade in food and feed products is the Appropriated Ecosystem Areas (ArEAs), which estimates the terrestrial and aquatic areas needed to produce all the inputs to particular products. The method is introduced in Paper I and used to calculate and track changes in imported subsidies to Swedish agriculture over the period 1962-1994. In 1994, Swedish consumers needed agricultural areas outside their national borders to satisfy more than a third of their food consumption needs. The method is then applied to Swedish meat production in Paper II to show that the term “Made in Sweden” is often a misnomer. In 1999, almost 80% of manufactured feed for Swedish pigs, cattle and chickens was dependent on imported inputs, mainly from Europe, Southeast Asia and South America. Paper III examines ecosystem subsidies to intensive aquaculture in two nations: shrimp production in Thailand and salmon production in Norway. In both countries, aquaculture was shown to rely increasingly on imported subsidies. The rapid expansion of aquaculture turned these countries from fishmeal net exporters to fishmeal net importers, increasingly using inputs from the Southeastern Pacific Ocean. As the examined agricultural and aquacultural production systems became globalized, levels of dependence on other nations’ ecosystems, the number of external supply sources, and the distance to these sources steadily increased. Dependence on other nations is not problematic, as long as we are able to acknowledge these links and sustainably manage resources both at home and abroad. However, ecosystem subsidies are seldom recognized or made explicit in national policy or economic accounts. Economic systems are generally not designed to receive feedbacks when the status of remote ecosystems changes, much less to respond in an ecologically sensitive manner. Papers IV and V discuss the problem of “masking” of the true environmental costs of production for trade. One of our conclusions is that, while the ArEAs approach is a useful tool for illuminating environmentally-based subsidies in the policy arena, it does not reflect all of the costs. Current agricultural and aquacultural production methods have generated substantial increases in production levels, but if policy continues to support the focus on yield and production increases alone, taking the work of ecosystems for granted, vulnerability can result. Thus, a challenge is to develop a set of complementary tools that can be used in economic accounting at national and international scales that address ecosystem support and performance. We conclude that future resilience in food production systems will require more explicit links between consumers and the work of supporting ecosystems, locally and in other regions of the world, and that food security planning will require active management of the capacity of all involved ecosystems to sustain food production.

L'augmentation de l'intérêt concernant la part des réservoirs hydroélectrique dans l'augmentation de la concentration atmosphérique des Gaz à Effet de Serre (GES) a amené à mesurer les émissions nettes d'un réservoir hydroélectrique, Nam Theun 2 (NT2) dans la région subtropicale de la République Démocratique Populaire du Laos, Asie. Ce travail est la première évaluation de l'empreinte carbone des GES (c'est à dire : les émissions après ennoiement moins les émissions avant ennoiement) en relation avec la création d'un réservoir hydroélectrique. C'est le résultat d'une étude à grande échelle qui s'est déroulée pendant cinq ans (2008-2012). Nous avons tout d'abord quantifié les sources et les puits majeurs des GES des composants terrestres et aquatiques du paysage avant ennoiement (Mai 2008). Ensuite, à partir d'Avril 2009, cette étude similaire a été réalisée au niveau du réservoir, sa zone de marnage et son aval. C'est en Octobre 2009 que le réservoir hydroélectrique NT2 a, pour la première fois, atteint son niveau maximal et c'est huit mois plus tard, en Mars 2010, que les turbines ont fonctionnées pour la première fois. En se basant sur un suivi bimensuel et sur cinq missions de terrain couvrant toutes les saisons, les émissions des principaux GES (c'est à dire l'oxyde nitreux (N2O), le méthane (CH4) et le dioxyde de carbone (CO2)) ont été mesurées d'Avril 2009 à Décembre 2011. Les émissions ont été déterminées à la surface du réservoir (flux diffusifs et ébullitifs) ainsi que dans les sols de la zone de marnage, qui peut atteindre 370 km2 pour une surface totale de réservoir de 450 km2.

Ce mémoire en recherche création débute par un récit poétique rédigé par fragments et intitulé Ma mère est une porte. Ce texte met en abyme les ruines afin de démontrer leur lien de contiguïté avec l’absence, l’empreinte, la mémoire et la mort. D’ailleurs, leur aspect ruiniforme rappelle le fragment, ce qui permet d’appuyer l’esthétique formelle de l’œuvre. La deuxième partie intitulée Approche génétique : un monument à la mémoire des disparus emportés par le temps de l’écriture, propose une réflexion sur l’imaginaire des ruines en lien avec les archives littéraires et la mémoire. Divisé en trois sections, cet essai pose un regard inquiet sur l’avenir de l’objet d’étude de la critique génétique, soit le manuscrit moderne. Depuis l’avènement de l’informatique et du traitement de texte, que conserverons-nous de la mémoire scripturale et du processus d’écriture de l’écrivain de demain? / This M.A. Thesis combining research and creative writing is divided in two parts: a poetic narrative and an essay. Ma mère est une porte is structured in fragments and contains the mise en abyme of ruins in order to demonstrate that they closely relate to the ideas of absence, genetic footprint, memory and death. Ruins suggest fragments, thus the narrative’s esthetical form. The second part, Approche génétique : un monument à la mémoire des disparus emportés par le temps de l’écriture, is an essay reflecting on the metaphorical link between manuscript, ruins, archives and memory. Divided in three sections, it posits that new technology will cause modern manuscripts to disappear and the methodology behind genesis of text’s to evolve. Moreover, this will change how creative writing’s memory is preserved, hence affecting our cultural heritage.

Despite the introduction of antiretroviral therapy, the HIV/HIV epidemic remains an unsolved global health problem. Amongst all the host defence mechanisms, HLA class I molecules have shown the strongest genetic association with delayed disease progression, in particular HLA-B alleles. Numerous studies have shown that the HLAmediated CD8+ T cell responses play a central role in the immune control of HIV. Yet our understanding of HLA-mediated immune control of HIV remains incomplete, even when considering the best-defined epitopes restricted by the protective HLA alleles at a population level. The studies I have conducted and describe herein focus on two well-charaterised protective HLA-B molecules, HLA-B*81:01 and HLA-B*27:05; a third protective molecule, HLA-B*52:01, that has not been well-studied hitherto; and finally the most prevalent HLAB allele in many Asian populations such as Taiwan, HLA-B*40:01, which has an apparently neutral effect on viral replication. This thesis is centred on the Gag-specific immune response, since previous studies have shown the benefits of CD8+ T-cell responses targeting this conserved and immunogenic region of the HIV proteome, in particular the p24 capsid protein. I have investigated here HLA footprints driven by CD8+ T-cell pressure on HIV that are evident in the viral sequences of individuals expressing these HLA molecules. These footprints include novel escape and putative compensatory mutations. The impact of these variants on viral replicative capacity (VRC) and on HIV disease outcome clinical outcomes was examined via fitness assays. These studies identified several escape mutations that effectively cripple HIV. The distinct compensatory pathways available to the virus to mitigate the fitness cost of particular escape mutations were evaluated. In the course of these analyses I have demonstrated the critical influence of the viral backbone, including HIV clade, in combination with particular viral variants, on VRC. Computational modelling analysis has been applied to facilitate understanding of the mechanism by which certain mutants affect the stability of interactions between HLA and viral capsid protein. This thesis offers novel insights into immune control of the key HIV subtypes – B- and C-clade – and of the most severely affected populations – in Africa (South Africa) and Asia (India and Taiwan) – within the global epidemic. This work helps to better define the viral mutation landscape that is essential both for future vaccines designed to corner the virus, and for successful HIV cure strategies.

The investigation used the Ecological Footprint as an educational tool to assist Environmental Management and Water Care learners at Technikon Northern Gauteng, Soshanguve learners in gaining insight in their consumption of natural renewable and non-renewable resources and generation of wastes. In addition, it also aimed to assist them in understanding the ecological impacts of their behavior on the available international and national productive land and thus on planet earth. The results of the investigation indicated a significant decrease in the post-test questionnaire knowledge, attitude, and behaviour scores of the learner group. The implication however was clear; the Ecological Footprint did not directly have an effect on the attitude and behaviour of the learners but indirectly influenced the knowledge base of the learners, which then should have an impact on their attitude and behaviour over time. The Ecological Footprint could thus be an educational tool that could be incorporated into the curricula of the two academic programs at TNG, viz Water Care and Environmental Management for increasing knowledge and improving the attitude and behaviour of learners towards a more sustainable lifestyle. / Educational Studies / M.Ed.(Environmental Education)

La normativa comunitaria ha favorito lo sviluppo di sistemi di qualità certificati. Oggi la CE sta guidando il sistema qualità verso un nuovo orizzonte: la valutazione delle prestazioni di sostenibilità di prodotti e organizzazioni, e lo sta facendo attraverso la metodologia dell'Impronta Ambientale (EF). A livello nazionale dal 2009 il Ministero dell'Ambiente sta promuovendo un intenso programma di valutazione delle prestazioni ambientali dei prodotti e di riduzione delle emissioni di gas serra delle imprese italiane. Una delle iniziative di maggior successo è il progetto VIVA "La Sostenibilità della vitivinicoltura in Italia". Con la pubblicazione del decreto Ministeriale n. 56 del marzo 2018 che approva lo schema volontario Made Green in Italy per l'applicazione della metodologia PEF in Italia, e con la pubblicazione del PEFCR per “Still and sparkling wine”. È chiaro che sia a livello nazionale che europeo la direzione intrapresa va verso la metodologia EF dalla Commissione Europea. Supponendo che il protocollo VIVA possa essere influenzato anche dall'evoluzione del metodo EF; sono state valutate le possibili implicazioni legate ad una futura transizione da VIVA alla PEF, effettuando anche uno studio PEF su 27 prodotti certificati VIVA con un confronto di prestazioni tra i prodotti VIVA e i benchmark europei. / EU regulations have favoured the development of certified quality schemes. Today the EC is driving the quality sector towards a new horizon; the evaluation of sustainability performance of product and organizations, and is doing it through the Environmental Footprint Methodology. At national level since 2009 the Italian Ministry for the Environment Land and Sea is promoting an intense programme for the evaluation of products’ environmental performances and for the reduction of Italian companies’ greenhouse gas emissions. One of the most successful initiative is the VIVA “Sustainability and Culture” project addressed to the wine sector. With the release of the IMELS decree n. 56 of march 2018 approving the Made Green in Italy Voluntary Scheme for PEF methodology application in Italy, and with the publication of the PEFCR for still and sparkling wine. Is clear that the direction taken at national and European level goes toward the EF methodology developed by the European Commission Assuming that the VIVA protocol may also be affected by EF evolution; possible implications linked to a future transition from VIVA to PEF were evaluated, also carrying out a PEF assessment of 27 VIVA certified products with a performance confrontation between the VIVA products and the European benchmarks.

Notre étude porte sur la preuve de concept de contrôle électrochimique de la méthanogénèse, métabolisme clé de la digestion anaérobie et de la bioremédiation des eaux, en exploitant le principe des piles à combustible microbiennes. Une première partie bibliographique vise à décrire les mécanismes de la méthanogénèse dans le contexte de l'auto-épuration des eaux et de production naturelle de gaz à effet de serre (GES). Les technologies de pile à combustibles microbiennes y sont traitées. Une analyse critique des études sur le contrôle électrochimique de la méthanogénèse permet de dimensionner un montage expérimental dédié à la quantification des GES en cultures biologiques électro-stimulées. Sa conception, sa validation ainsi que les méthodes de mise en culture sont décrites dans une seconde partie. Une série de cultures préliminaires sur des boues digérées anaérobies de station d'épuration permettent d'identifier et fixer les paramètres expérimentaux. Dans une troisième partie, une étude expérimentale fait la preuve de concept de contrôle électrochimique de la méthanogénèse avec une diminution significative de 33% en CH4 (tension de +300 mV vs Ag/AgCl) par rapport à la méthanogénèse naturelle non stimulée. Toutefois, la stimulation contribue à multiplier par 10 la production de CO2. Ce constat amène la problématique supplémentaire d'impact sur l'effet de serre des cultures étudiées. Nous allons donc plus loin que l'objectif initial en nous intéressant à l'empreinte carbone générée par l'ensemble des GES. Le traitement électrochimique, outre la diminution du CH4 produit, permet de diminuer la contribution à l'effet de serre de 15% des cultures électro-stimulées / Our study focuses on the proof of concept of electrochemical control of methanogenesis, key metabolism of anaerobic digestion and water bioremediation, using the principle of microbial fuel cells. A first bibliographic section aims to describe the mechanisms of methanogenesis in the context of self-purification of water and natural production of greenhouse gases (GHG). Microbial fuel cell technologies are addressed. A critical analysis of the studies dealing with electrochemical control of methanogenesis makes it possible to size an experimental setup dedicated to quantification of GHGs in electro-stimulated biological cultures. Its design, validation and methods of cultivation are described in a second part. A series of preliminary cultures on anaerobic digested sewage sludge make it possible to identify and set the experimental parameters. In a third part, an experimental study proves the concept of electrochemical control of methanogenesis with a significant decrease of 33% in CH4 (voltage of +300 mV vs Ag/AgCl) compared to natural unstimulated methanogenesis. However, stimulation contributes to a 10-fold increase in CO2 production. This observation leads to the additional problem of impact on the greenhouse effect of the cultures studied. We go further than the initial objective by looking at the carbon footprint generated by all GHGs. The electrochemical treatment, in addition to the reduction of CH4 produced, makes it possible to reduce the contribution to the greenhouse effect of 15% of electro-stimulated cultures

Until recently, the concept of green logistics has been disregarded by various logistics and transport companies in South Africa. The study on which this dissertation is based explored the green logistics practices that these companies are currently implementing in terms of the key drivers, benefits and barriers. A quantitative research approach was followed, were a survey (Lime) served as the primary research instrument. A census was conducted among 160 companies in Gauteng. The results of the study revealed a significant difference between SMEs (<200) and large (200 and above) companies with regard to their importance rating on green logistics practices. To achieve the primary objective of the study, a framework in green logistics was drafted for SMEs and large companies in South Africa, which outlined practices and opportunities companies can implement in their own businesses to benefit from ‘going green’. The usefulness of the latter mentioned guidelines needs to be tested in future research. / Public Administration and Management / M.. Com. (Logistics Management)