No description available.

No description available.

Non-equilibrium plasma modification of polymer surfaces in an oxygen atmosphere provides a highly efficient, solventless method of raising the surface energy. The chemical and physical effects of non-equilibrium plasma treatment on polymer surfaces have been investigated. Oxygen glow discharge and silent discharge treatment of several polymers (polypropylene, polystyrene, polyphenylene oxide and polycarbonate) has been shown to cause both surface oxidation and chain scission at the polymer surface. This generates low molecular weight oxidised material on the polymer surface which conglomerates into globular features due to the difference in surface energy between the oxidised material and the untreated polymer. These features can be removed by solvent washing. Generally silent discharge treatment generates more low molecular weight oxidised material whereas oxygen glow discharge treatment generates more non-soluble oxidised material. Crystalline polymers react at a slower rate than amorphous material. During the treatment of a model crystalline polymer (hexatriacontane) the plasma attacks the edges of the crystal, rather than the surface, due to the greater chain mobility at the edge. Non-equilibrium plasma treatment of both miscible and immiscible polymer blends were investigated. The size and distribution of the globular features formed were found to be dependent on the blend composition. For the immicible polymer blend, non-equilibrium plasma treatment reveals the blend morphology mi sing from the difference in reaction rates of the parent polymers.

No description available.

It is becoming clear that mechanical stimuli are critical in regulating cell biology; however, the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells expressing actin-EGFP with controlled forces (0-20nN) in order to investigate the cell’s structural response. Two clear force dependent responses were observed: a short-term local deformation of actin stress fibres and a long-term force-induced remodelling of stress fibres at cell edges, far from the point of contact. We were also able to quantify strain dynamics occurring along stress fibres. The cell exhibits complex heterogeneous negative and positive strain fluctuations along stress fibres, indicating localized dynamic contraction and expansion. A ~50% increase in myosin contractile activity is apparent following application of 20nN force. Directly visualizing force-propagation and stress fibre strain dynamics has revealed new information about the pathways involved in mechanotransduction which ultimately govern the downstream response of a cell.

Maximum voluntary forces and psychophysically acceptable forces are often used to set force guidelines for exertions as a means to protect against overexertion injuries in the workplace. The focus of this dissertation was the exploration of the roles of whole body balance, shoe-floor friction and joint strength in limiting the capacity of a person to produce maximum voluntary hand forces and psychophysically acceptable hand forces. The underlying goal was to advance knowledge regarding how physical exertion capacity is biomechanically governed, then to use this information to develop models to predict capability based on these governing principles. The hypothesis underscoring this work was that maximum voluntary hand force capability is governed by whole body balance, shoe-floor friction and joint strength; and consequently, psychophysically acceptable forces would be chosen proportionally to this maximum voluntary force capability, where the magnitude of the proportionality was dependent on the limiting factor, or ‘weakest link’. To investigate this hypothesis, both experimental and mathematical modeling paradigms were used. Initially, an experimental study was used to investigate how biomechanical factors governed maximum hand force capability across a range of exertions. It revealed that each governing factor differentially limited maximum force capability. Moreover, this study identified how foot placement, handle height, distance from the handle, friction, and body posture all influence the underlying biomechanical weakest link, and ultimately force producing capability. Data gathered in the experimental study was next used to evaluate a mathematical model that was developed to predict maximum force capability, given information on posture and direction of force application. In addition, the model also predicted population variability in maximum capacity based on the inclusion of a novel approach to probabilistically represent population variability. The evaluation demonstrated that the model underestimated maximum hand force capability compared to measured hand forces by approximately 18, 26, and 41% during medial, pulling and downward exertions respectively. However, it appeared that the ‘weakest link’ principle for predicting maximum force capacity was plausible, as evidenced by significant rank ordered correlations between the measured and predicted hand forces. Further research investigated if psychophysically acceptable forces were selected as a proportion of task specific maximum voluntary force capability, where the proportionality was related to the biomechanical weakest link. Using an experimental design, psychophysically acceptable forces and corresponding maximum forces were measured. Participants chose psychophysically acceptable forces that were 4/5ths of their task specific maximum voluntary force capability when capability was limited by balance. Additionally, they choose psychophysically acceptable forces that were 2/3rds of their maximum voluntary force capability when capability was limited by joint strength. The identification and confirmation of a weakest link proportionality principle represents an important contribution to the field of occupational biomechanics. The weakest link proportionality principle was integrated into the model to allow prediction of: maximum voluntary hand force capability, the limiting factor, and psychophysically acceptable hand force capability. The updated model underestimated empirically measured psychophysically acceptable forces by 24% and 43% during downward and pulling exertions respectively. However, the original model underestimated the maximum hand force capacity by 23% and 34% during the same exertions, without the proportional relationships. This underestimation may be a result of the underlying assumption that joint strength is independent, resulting in an underestimation of maximum joint strength capacity and a corresponding underestimation of maximum hand force capacity. The underestimation may also be due to differences in strength capacities between the participants tested during this thesis compared to those tested in past research used to determine the maximum strength indices reported in the literature. This body of work supported the hypothesis that psychophysically acceptable forces are selected as a proportion of the maximum voluntary hand force, where the proportionality depends on the underlying biomechanical weakest link. The model is a promising first step towards predicting maximum and psychophysically acceptable occupational force threshold limits.

Alzheimer's Disease (AD) is a neurodegenerative disorder that is prevalent among the elderly population. Aß protein has been heavily implicated in the pathogenesis of AD. This protein in its fibrillar form is a major component in the senile plaques that form on neuronal cellular membranes during the course of AD. Despite substantial efforts the exact mechanism of Aß toxicity towards a cell membrane is not well-understood. The determination of this mechanism, however, is of utmost importance, since the membrane presents the first site of Aß interaction with neurons, which in turn maybe the origin of Aß neurotoxicity. The purpose of this study was to find a lipid composition that can be used as a model of neuronal membrane for subsequent studies of the role of topographical heterogeneity (domain formation) on Aß-membrane interaction as related to AD. The lipids used in the study were 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), cholesterol (Chol), sphingomyelin (SM) and ganglioside GM1 (GM1). These lipids were combined in different proportions and deposited on a mica substrate to form supported monolayers. They were then imaged with an atomic force microscope (AFM) to determine if any of them exhibited domain formation. Three of the studied samples: POPC/POPG/SM 40:40:20 +5%Chol, POPC/SM/Chol 75:20:5 and POPC/SM/GM1/Chol 74:2:1:23 were found to possess interesting topography, rich in structural features: pores and domains. The average height difference between the domain features for each sample was found to be 0.58±015 nm, 0.61±0.12 nm and 0.27±0:07 nm.

A major challenge to any “solvent-based” bitumen extraction technology is the removal of suspended fine solids from the hydrocarbon medium (i.e. diluted bitumen). To address this problem, we examined how colloidal solids could be made to aggregate in a hydrocarbon medium and thus be separated by gravity settling. The model solids were micron-sized “bitumen-treated” silica particles; the oil phase was bitumen diluted in an organic solvent of variable aromatic content. On the macroscopic scale, the experiments involved quantifying the settling rates of the particles as the aromatic content of the solvent was varied. Our results showed the existence of an optimal (non-zero) aromatic content at which the solids settling rate was the highest. On the microscopic scale, adhesive forces between individual glass spheres were directly measured using the microcantilever technique (again in non-aqueous media). It was demonstrated that, in addition to being captured by asphaltene networks, the suspended solids could also homo-flocculate — and thus form aggregates and be separated — in an alkane-diluted bitumen environment. / Chemical Engineering

Thesis (PhDBiomedicalScience)--University of South Australia, 2001.

Thesis (PhD)--University of South Australia, 1997