Contemporary scholars seeking to increase Tuber Melanosporum truffle production rely almost exclusively on technological advancements to increase yields, while failing to place the cultivation of truffles, trufficulture, in its historical or local landscape contexts. In this dissertation, I describe how truffle scholars’ conceptualization of trufficulture and landscapes has changed over 150 years in France, while focusing on the French département of Lot. I examine changing relations between humans and nonhumans and how they impact truffle harvests. I analyzed the history of French trufficulture through a close reading of historic truffle manuals, archival research and the classification of remotely sensed images. Shifting from the past to the present, from July 2014-August 2016, I conducted semi-structured survey interviews with working truffle-growers (trufficulteurs) and participant observation at meetings of trufficulteurs, truffle hunts and truffle markets. I utilize actor-network theory (ANT) as both a theory and methodology. Actor-network theory allowed me to follow the impacts made by both humans and nonhumans on trufficulture. I found that truffle harvests in the 1880s dropped by 90%. Highly populated, intensively worked landscapes of viticulture, silvopastoralism and cereal cultivation created conditions suitable to truffles. By the 1870s the phylloxera aphid ravaged grapevines, which made trufficulture an important source of revenue. These advantageous conditions would not last. Post-WWI, yields fell for decades because of an ongoing rural population exodus and consequent agricultural abandonment, which promoted reforestation and closed canopy forests in Lot, France. By the 1960s, French trufficulteurs organized associations to share knowledge and promote local truffle markets to revive production. Trufficulteurs’ utilization of tractors, ‘inoculated’ plants and irrigation systems produced a new form of “modern” trufficulture. State subsidies helped trufficulteurs adopt “modern” practices, in hopes of increasing yields. “Modern” trufficulture has not dramatically increased yields. A few highly-capitalized trufficulteurs dominate production in Lot. Many others practice trufficulture as a hobby. Instead of relying on “modern” technological fixes, my findings suggest that trufficulteurs, farmers and states should reinvigorate extensive polyculture farming practices that maintain open canopy forests, which were beneficial to trufficulture in the past. Actor-network theory allowed me to rethink human and nonhuman relations, and to propose alternatives to “modern” trufficulture.