Thesis (M.A.)--University of Wisconsin--Madison, 1945. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 42-43)

The main objective of the present study was to determine whether the varieties of Dacrymyces deliquescens sensu Kennedy represent a single species or are three distinct species, and to study the life cycles of the fungi of this complex. An unsuccessful attempt was made to grow these fungi through their life cycles in culture. Cultural characteristics were compared among the varieties as well as to those characteristics reported in the literature. To obtain single spore cultures for mating tests, eight methods and six media were tried without successful results. Of the 1560 spores isolated only three resulted in mycelial growth. Erroneous isolation and the presence of a number of spores were suspected in these cultures. The development of the basidium and of basidiospores were studied cytologically to determine the fate of the two nuclei remaining in the basidium. The two supernumerary, nuclei remain in the hypobasidium and degenerate. Conidia produced on the mycelium are globose, elliptical or oval for D. deliquescens (Merat) Duby var. deliquescens Kennedy, D. deliquescens var.ellisii (Coker) Kennedy and D. deliquescens var. minor (Peck) Kennedy respectively. It is suggested that these conidia are carried away by water rather than ejected by some mechanism, as has been previously proposed. Color differences among cultures and different shape of conidia produced indicate a definite degree of separation while intermediate forms among the three organisms indicate close relationship. Whether there are three species or one species with three varieties involved cannot be determined without mating tests. The suggested life cycle for D. deliquescens var. deliquescens follows: Basidiospores are uninucleate at the time of discharge. The nucleus soon divides and 3-septate, four-celled spores are formed. Germination is induced by a volatile substance which is produced in minute quantities. Cells of basidiospores act independently after germination of the spore. Spores germinate by germ tubes, by conidia or by a combination of the two. Production of conidia terminates the germination of individual cells. The maximum number of germ tubes produced by any one cell is two. Monokaryotic mycelium is produced upon germination of basidiospores and conidia. Dlkaryotization takes place in an unknown way. Arthrospores may be produced on dikaryotic mycelium, and these germinate to produce dikaryotic mycelium. Basidia are formed on dikaryotic mycelium. The young basidium is cylindric, binucleate, and separated from the hyphae by a basal septum. A fusion nucleus is produced which gives rise upon division to four stichobasidially arranged daughter nuclei. One nucleus passes into each epibasidium and this later becomes the nucleus of the spore. Two nuclei remain in the hypobasidium and degenerate. / Science, Faculty of / Botany, Department of / Graduate

[Abstract Omitted] / Science, Faculty of / Botany, Department of / Zoology, Department of / Graduate

The fluorescent antibody technique was investigated as a means of facilitating the recognition and identification of the fungal components of a western red cedar (Thuja plicata Donn) heartwood flora in situ. Fungi isolated from the heartwood were grown in bulk and prepared for two different injection trials. In one trial the antigen was the particulate matter of the cell that could be centrifuged into a pellet after the hyphae were destroyed by a tissue grinder. In the second trial the hyphae were ground up and ultrasonically disintegrated. Only the cytoplasm and small wall fragments were retained for injection. After antisera collection the indirect staining method was employed. Unlabeled specific antiserum was layered over the antigen, allowed to incubate and washed off before fluorescent sheep anti-rabbit globulin was applied to form the final layer. All attempts to detect specific antibodies to the fungal antigens failed. This was probably due to not using antigens rich enough in protein. Successful production of precipitating antibodies to fungal antigens has been shown by other workers to be more likely when the antigen contains greater than 10 milligrams of protein per milliliter of antigen solution. It has also been found that in some cases fresh antigen must be prepared for each diffusion test and injection as it can't be preserved even at -20°C. It is hoped that if fresh, high protein antigen were to be used this study could be successfully completed. / Forestry, Faculty of / Graduate

No description available.

Thesis (Ph.D.)--York University, 2003. Graduate Programme in Biology. / Typescript. Includes bibliographical references (leaves 124-163). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNQ86363

The inclusion of the species of polypores with rose-colored context in the genus Fomitopsis is proposed in this study. Temperature responses demonstrated that these fungi can be placed in three groups according to their optimum temperature for growth: (1) Fungi with an optimum at 25 C, Fomitopsis rosea; (2) Fungi with an optimum at 28 C, Fomitopsis feei, Fomitopsis lilacino-gilva, Fomitopsis cajanderi, and Fomitopsis carnea; (3) Fungi with an optimum at 30-34 C, Fomitopsis dochmius, F. cajanderi, and Fomitopsis cupreo-rosea. Decay tests showed that Fomitopsis feei and Fomitopsis lilacino-gilva reported only on hardwoods are also capable of decaying conifers "in vitro", causing weight losses up to 40% after 20 weeks. It was confirmed that all these fungi were brown rotters. An heterothallic, bipolar behavior was demonstrated for F. feei and agreed with the behavior previously reported for two other members on this complex, F. rosea and F. cajanderi. Due to the failure of the other isolates to fruit in culture, their sexual behavior could not be determined. Monokaryotic x monokaryotic pairings and monokaryotic x dikaryotic pairings showed homogenic or heterogenic incompatibility between members of the same species or different species. Vegetative incompatibility was found when pairing heterokaryotic dikaryons of different isolates of the same species which demonstrated genetic diversity in the populations. Dikaryotization did not occur when heterokaryotic dikaryons of F. lilacino-gilva, F. dochmius, F. carnea, and F. cupreo-rosea were paired with monokaryons of F. feei, F. cajanderi, or F. rosea which supported the concept of genetically different species.

No description available.

Thesis (Ph. D. - Plant Pathology)--University of Arizona. / Includes bibliographical references.

Thesis (Ph. D.)--University of Adelaide, Dept. of Soil and Water, 2000? / Includes bibliographical references (leaves 138-160).