<p dir="ltr">This research is about the innovative method for the control of the one of the fungal disease, Fusarium head blight, in wheat.</p><p dir="ltr"><i>Fusarium graminearum</i> is a devastating fungus that causes Fusarium head blight (FHB) disease in cereal crops, including wheat, rice, barley, and oats, as well as ear rot and stalk rot in maize. Currently, application of synthetic agrochemical fungicides remains the major mitigation strategy for FHB disease control. However, fungicide treatment carries risks to human health and the environment. Spray-induced gene silencing (SIGS) is suggested as an effective, sustainable, and environmentally friendly alternative for the control of fungal diseases. SIGS uses double-stranded RNA (dsRNA) to induce RNAi against eukaryotic pathogens and pest’s genes. Unlike fungicides, dsRNA can be engineered and carefully designed to target specific fungal pathogens, essentially limiting off-target effects and reducing harm to non-target organisms, including beneficial species.</p><p dir="ltr">In this study, we investigated the <i>in vitro</i> and <i>in vivo</i> effects of dsRNA application on growth and pathogenicity of <i>Fusarium graminearum</i> (<i>Fg</i>). We designed dsRNA against eight fungal genes <i>FgMGV1</i>, <i>FgRAS1</i>, <i>FgCOT1</i>, <i>FgPp2A</i>, <i>FgCAK1</i>, <i>FgTRI5</i>, <i>FgGMK1</i>, and <i>FgYCK1</i>, which have been previously reported to have a functional role in fungal growth and pathogenicity. I postulated that silencing the transcript expression of these selected fungal genes will lead to an attenuation of <i>F</i>. <i>graminearum</i> growth, development and virulence. For lab-scale <i>in vivo</i> dsRNA production, we first designed dsRNAs for each of the fungal genes using the pssRNAit webserver. These gene fragments were subsequently cloned into the L4440 plasmid and transformed into the RNAase III-deficient <i>E</i>. <i>coli</i> strain <i>HT115</i>(DE3). Induction of dsRNA production was mediated by the addition of Isopropyl-β-D-thiogalactoside (IPTG). Graphene Quantum Dots (GQDs) were used as nanocarrier, which were synthesized by pyrolyzing citric acid coupled with surface functionalization by using branched polyethyleneimine (bPEI).</p><p dir="ltr">The performance of ds<i>TRI5</i>, ds<i>MGV1</i>, ds<i>YCK1</i> and ds<i>COT1</i> in reducing fungal biomass in SNA medium was better than dsRNA of other genes, as evidence by lowering fungal biomass by almost half, while ds<i>CAK1</i> and ds<i>GMK1</i> being the least effective in controlling biomass. The inhibitory effect of dsRNA on fungal growth in plate was also investigated. The mycelial growth of <i>F. graminearum</i> on plates was highly inhibited with distinct inhibition zones when plate media was made by mixing the dsRNAs. When wheat spikes of two varieties i.e., ‘AL105’ and ‘Gilat’ were point inoculated with <i>F</i>. <i>graminearum</i>, and after three days sprayed with dsRNAs, the percent symptomatic spikelets (PSS) significantly reduced to 20-25% in both varieties while non-treated control spikes showed 100% symptomatic spikelets. We also evaluated the effect of dsRNA on lowering the accumulation of mycotoxin deoxynivalenol (DON) post-infection followed by the analysis of transcript abundance for two genes <i>FgRAS1</i> and <i>FgPP2A</i> after the application of dsRNA in the liquid culture.</p><p dir="ltr">In summary, we have provided the first example of utilizing graphene quantum dots, for the delivery of dsRNA in SIGS applications in wheat against <i>Fusarium graminearum</i>. In addition, exogenously applied dsRNA degrades naturally, leaving no harmful chemical residues on plants, soil, or water bodies, making it safer for human consumption and the environment. Further optimization of delivery systems for improving the uptake of pathogens efficiency should be done before commercial use of RNA-based disease management in fields.</p>

The present study analyzes the economic impact of the introduction of Genetically Modified Organisms (GMOs) in Uruguay's agriculture. Using a partial equilibrium framework the impacts of transgenic varieties are simulated for two crops, rice and potatoes, in small open and closed economies respectively. The model accounts for the presence of market imperfections created by the monopolistic behavior of the genes' patent owner. The change in economic surplus generated after the adoption of the new technology is projected to be positive, although the seed markup charged by the monopolist reduces the surplus compared to a perfectly competitive market. Total deadweight losses and domestic losses are found to increase with the seed premium, as additional monopolist profits are extracted out of the country. Adoption decreases with the seed premium, further reducing the domestic consumer and producer surplus. The results of the study suggest an active role for national technology policies and for the agricultural R&D system in Uruguay to generate conditions that attract the technology's owner to a small market while at the same time reducing the potential losses that monopoly power creates . / Master of Science

Boston University. University Professors Program Senior theses. / PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. / 2999-01-01

Legal Regulation of the Use of Genetically Modified Organisms ABSTRACT The use of genetically modified organisms on a global scale is on the rise which requires their efficient and consistent regulation. The main goal of the thesis is to provide a comprehensive overview of the legal regulation of genetically modified organisms and their use on several levels. After an introduction to the topic, the thesis deals with the key international documents and then moves on to examining different approaches on the topic and exploring regulation in the United States and in the European Union with a connection the legislation in Czech Republic. In addition, four annexes are included at the end of the thesis for a better illustration of the current use of genetically modified organisms in the world.

No description available.

Darbo tikslas: Įvertinti KTU Cheminės technologijos fakulteto studentų žinias ir požiūrį apie genetiškai modifikuotą maistą, jo ženklinimą. Darbo uždaviniai: 1. Nustatyti KTU Cheminės technologijos fakulteto studentų informavimo šaltinius apie genetiškai modifikuotą maistą ir jo ženklinimą. 2. Ištirti KTU Cheminės technologijos fakulteto studentų žinias ir požiūrį apie genetiškai modifikuotą maistą ir jo ženklinimą. 3. Parengti rekomendacijas siekiant pagerinti genetiškai modifikuoto maisto ir jo ženklinimo informuotumą. Tyrimo metodika: Tyrimo objektas: Žinios ir požiūris apie genetiškai modifikuotą maistą ir jo ženklinimą. Tyrimo metodai: Mokslinės literatūros analizė, teisinių dokumentų analizė, anketinė apklausa. Tyrimo metu buvo išplatinta 120, atsako dažnis - 80,83 proc. Statistinės analizės metodai: Apklausos rezultatai buvo analizuojami naudojant Microsoft office Excel 2007 ir kompiuterinį SPSS 13.0 versijos statistinį duomenų analizės paketą. Statistinis duomenų reikšmingumas buvo tikrinamas pagal chi kvadrato kriterijų ir laisvės laipsnių skaičių. Požymius analizuojant kito rodiklio atžvilgiu skirtumai laikyti statistiškai reikšmingais, kai reikšmingumo lygmuo p<0,05. Rezultatai: Dauguma respondentų dažniausiai gauna informaciją apie genetiškai modifikuotą maistą ir jo ženklinimą internete (15,5 proc. moterų ir 11,3 proc. vyrų), 30,9 proc. moterų ir 35,1 proc. vyrų žino šiek tiek, tačiau norėtų būti daugiau informuojamas,-a. Didžioji dalis apklaustųjų mano, jog... [toliau žr. visą tekstą] / Aim of study: to evaluate student‘s knowledge and attitude to genetically modified food and its labeling in KTU faculty of chemical technology. Objectives: 1. To survey the repositories of genetically modified food and his labeling. 2. To survey the knowledge and attitude to genetically modified food and its labeling. 3. To prepare recomendations to increase privity of genetically modified food and its labeling. Methods: Object of the research - knowledge and attitude to genetically modified food and its labeling. The questionnaire was compiled after making an analysis of the latest Lithuanian and foreign scientific literature and legislation. There were totally 120 students of KTU faculty of chemical tecnology interviewed using the anonymous questionnaire (response rate 80,83 percentage). Data analysis was performed using the statistical data analysis package SPSS (version 13.00) (the chi-squared test, df) and Microsoft office Excel 2007. Differences between the indices were considered statistically significant for p-values less than 0.05. Results: The most of respondents get information about genetically modified food and its labeling commonly in internet (15.5 percentage women and 11.3 percentage men), 30.9 percentage women and 35.1 men know a little bit, but they want to get more information. In addition to this, respondents think, that genetically modified food is harmful (33.7 percentage women and 32.3 percentage men) because of lack information about its safety (33.6... [to full text]

Universiẗat, Diss., 2003--Freiburg. / Includes bibliographical references (p. [429]-454) and index.

Diss. (sammanfattning) Uppsala : Univ., 2004. / Härtill 4 uppsatser.

Os panoramas social e ambiental estão intimamente relacionados, o que atrai a perspectiva socioambiental. Assim, as interferências nas condições de vida da população proporcionadas pela biotecnologia são compreendidas na proteção ao meio ambiente. A comercialização de organismos geneticamente modificados em alimentos gera muito debate doutrinário e científico na atualidade. No entanto, já é possível revelar alguns mitos e efetivar algumas constatações acerca do tema. Os mitos trazem as promessas de buscar saciar a fome através do aumento da produção com a utilização da biotecnologia na área alimentar, bem como a prioridade na produção de alimentos com propriedades benéficas para a saúde da população. As constatações esbarram em alguns episódios negativos envolvendo a utilização de organismos geneticamente modificados e, também, no forte domínio de grandes corporações, que estão ditando as regras do desenvolvimento biotecnológico. Diante deste quadro, na comercialização dos alimentos, a disponibilização de informações através da rotulagem ganha destaque, sendo importante analisar o tratamento legal que vem sendo dado no âmbito interno, na União Européia, sob a perspectiva dos Estados Unidos e do Codex Alimentarius. Diante do contexto legal, resta claro que ainda existem diversas polêmicas e que a disponibilização de informações ainda encontra alguns desafios, em especial, na fiscalização da rotulagem e na conciliação da informação com a diversidade cultural. Para que a informação tenha plena concretização deve estar inter-relacionada com a educação e a participação dos atores envolvidos. / Submitted by Marcelo Teixeira (mvteixeira@ucs.br) on 2014-06-02T19:26:59Z No. of bitstreams: 1 Dissertacao Patricia Maria Schneider.pdf: 760958 bytes, checksum: a2b8997a6ff8084e112826104e2cbf4a (MD5) / Made available in DSpace on 2014-06-02T19:26:59Z (GMT). No. of bitstreams: 1 Dissertacao Patricia Maria Schneider.pdf: 760958 bytes, checksum: a2b8997a6ff8084e112826104e2cbf4a (MD5) / The social and environmental views are closely related, which attracts the socioenvironmental perspective. All artifacts in the living conditions of the population is comprised of biotechnology in environmental protection. The commercialization of genetically modified organisms in food generates much debate and scientific doctrine today. However, it is possible to draw some myths and commit some observations on the subject. Myths bring the promises of seeking satisfying hunger by increasing production with the use of biotechnology in the food, as well as the priority in food production with properties beneficial to health. The findings run into some negative episodes involving the use of genetically modified organisms and also strong in the domain of large corporations that are dictating the rules of biotechnology development. This picture, in the marketing of food, the availability of information through labeling is emphasized, it is important to consider the legal treatment that has been given at the domestic, European Union, from the perspective of the United States and the Codex Alimentarius. Given the legal context, it remains clear that there are still many controversies and the availability of information is still some challenges, particularly in reviewing the labeling and reconciliation of information about cultural diversity. For information to have full effect should be inter-related education and participation of stakeholders.

Os panoramas social e ambiental estão intimamente relacionados, o que atrai a perspectiva socioambiental. Assim, as interferências nas condições de vida da população proporcionadas pela biotecnologia são compreendidas na proteção ao meio ambiente. A comercialização de organismos geneticamente modificados em alimentos gera muito debate doutrinário e científico na atualidade. No entanto, já é possível revelar alguns mitos e efetivar algumas constatações acerca do tema. Os mitos trazem as promessas de buscar saciar a fome através do aumento da produção com a utilização da biotecnologia na área alimentar, bem como a prioridade na produção de alimentos com propriedades benéficas para a saúde da população. As constatações esbarram em alguns episódios negativos envolvendo a utilização de organismos geneticamente modificados e, também, no forte domínio de grandes corporações, que estão ditando as regras do desenvolvimento biotecnológico. Diante deste quadro, na comercialização dos alimentos, a disponibilização de informações através da rotulagem ganha destaque, sendo importante analisar o tratamento legal que vem sendo dado no âmbito interno, na União Européia, sob a perspectiva dos Estados Unidos e do Codex Alimentarius. Diante do contexto legal, resta claro que ainda existem diversas polêmicas e que a disponibilização de informações ainda encontra alguns desafios, em especial, na fiscalização da rotulagem e na conciliação da informação com a diversidade cultural. Para que a informação tenha plena concretização deve estar inter-relacionada com a educação e a participação dos atores envolvidos. / The social and environmental views are closely related, which attracts the socioenvironmental perspective. All artifacts in the living conditions of the population is comprised of biotechnology in environmental protection. The commercialization of genetically modified organisms in food generates much debate and scientific doctrine today. However, it is possible to draw some myths and commit some observations on the subject. Myths bring the promises of seeking satisfying hunger by increasing production with the use of biotechnology in the food, as well as the priority in food production with properties beneficial to health. The findings run into some negative episodes involving the use of genetically modified organisms and also strong in the domain of large corporations that are dictating the rules of biotechnology development. This picture, in the marketing of food, the availability of information through labeling is emphasized, it is important to consider the legal treatment that has been given at the domestic, European Union, from the perspective of the United States and the Codex Alimentarius. Given the legal context, it remains clear that there are still many controversies and the availability of information is still some challenges, particularly in reviewing the labeling and reconciliation of information about cultural diversity. For information to have full effect should be inter-related education and participation of stakeholders.