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No. of bitstreams: 5 60.pdf: 417283 bytes, checksum: 6813899edea0a249245bbb9ba9654b3b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Empresa de Pesquisa Agropecu?ria de Minas Gerais (EPAMIG) / O teste LERCAF? consiste na imers?o de sementes de caf? em solu??o de hipoclorito de s?dio. O cloro ativo, princ?pio ativo da solu??o, reage com o endosperma das sementes, identificando regi?es mortas ou lesionadas, colorindo-as de verde escuro. A partir da avalia??o da localiza??o da regi?o colorida, ? poss?vel classificar as sementes como vi?veis ou n?o vi?veis. O teste ? r?pido e de opera??o simples, mas a metodologia necessita ser testada para obter melhor precis?o e exatid?o dos resultados. Objetivou-se com este trabalho, adequar a metodologia do teste LERCAF? na determina??o da viabilidade de sementes de caf? (Coffea arabica L.), al?m de avaliar o perfil isoenzim?tico em sementes submetidas ao teste LERCAF?. Em um primeiro experimento, avaliou-se a efici?ncia do teste LERCAF? na determina??o da viabilidade em sementes de caf? das cultivares Catua? Amarelo IAC 44, Mundo Novo IAC 376-4, Travessia MGS, e Rubi MG 1192, para isso utilizaram-se solu??es de hipoclorito de s?dio com teores de 2,5%; 3,5%; 5,0% e 6% de cloro ativo e os per?odos de imers?o de 2, 3 e 6 horas, a 30 ?C. Observou-se pela caracteriza??o do perfil das cultivares, que a velocidade de germina??o n?o variou entre as cultivares, no entanto houve superioridade na germina??o da cultivar Rubi em rela??o ? Catua? Amarela e Travessia. No entanto, pelo teste LERCAF? foi poss?vel apenas ? separa??o das cultivares em dois n?veis de qualidade, por meio dos tratamentos 2,5% por 3 h, 3,5% por 2 h e 3 h, sendo as cultivares Rubi, Travessia e Mundo Novo de qualidade superior em rela??o a cultivar Catua? Amarelo. Na concentra??o de 2,5% de hipoclorito de s?dio por 2 horas, as sementes n?o apresentaram colora??o esverdeada no endosperma. J? nas concentra??es de 2,5% por 6 horas, 5% e 6% por 2h e 3h foi observada colora??o intensa dificultando a avalia??o das sementes. Na busca da adequa??o da metodologia do teste LERCAF?, foi realizado um segundo experimento, utilizando um lote de sementes de caf? da cultivar Catua? Vermelho IAC 99, neste experimento foi realizado a quantifica??o do teor de cloro ativo da solu??o de hipoclorito de s?dio e posteriormente avaliada a efici?ncia do teste, utilizando-se concentra??es de 1%, 2%, 3%, 4% e 5% de cloro ativo e per?odos de 1, 2, 3, 4 e 5 horas, a 30 ?C, tamb?m foi avaliado o perfil isoenzim?tica para enzimas Esterase (EST), Malato Desidrogenase(MDH), Super?xido Dismutase (SOD), Catalase (CAT) e ?lcool Desidrogenase (ADH). O teste LERCAF? permite a determina??o do potencial fisiol?gico das sementes de caf?, quando se utiliza solu??o de hipoclorito de s?dio quantificada, pelos tratamentos onde as sementes s?o imersas em solu??o com teor de 2% de cloro ativo pelo per?odo de 5 horas e 3% de cloro ativo pelo per?odo de 3 horas, a 30?C. As sementes de caf? submetidas ao teste LERCAF? apresentam altera??es na atividade das enzimas EST, MDH, SOD, CAT e ADH, sendo que a ativa??o ou desativa??o destes sistemas enzim?ticos s?o vari?veis com a concentra??o e tempo de imers?o na solu??o de cloro ativo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT The LERCAF? test consists in the immergence of coffee seeds in sodium hypochlorite solution. The active chloride, active component of the solution, reacts with the endosperm of the seeds, identifying dead or injured regions, staining them dark green. From the colored region location evaluation, it is possible to classify the seeds as viable or non-viable. The test is quick and of simple transaction, however, the methodology needs to be tested in order to obtain better result precision and accuracy. The objective of this work was to adjust the methodology of the LERCAF? test in determining the viability of coffee (Coffea Arabica L.) seeds, in addition to evaluating the isoenzymatic profile of seeds submitted to the LARCAF? test. A first experiment evaluated the efficiency of the LERCAF? test in determining the viability of coffee seeds of cultivars Yellow Catuai IAC 44, Novo Mundo IAC 376-4, Travessia MGS and Rubi MG 1192. In order to do this, we used sodium hypochlorite solutions with active chloride contents of 2.5%, 3.5%, 5.0% and 6.0% and immersion periods of 2, 3 and 6 hours, at 30 oC. By the characterization of the cultivar profiles, we observed that germination speed did not vary between the cultivars, however, there was superiority in cultivar Rubi germination in relation to Yellow Catuai and Travessia. However, by the LERCAF? test, only the separation of the cultivars in two quality levels was possible, with the treatments 2.5% for 3 h, 3.5% for 2 h and 3 h, with cultivars Rubi, Travessia and Mundo Novo of superior quality in relation to Yellow Catuai cultivar. At the concentration of 2.5% of sodium hypochlorite for 2 hours, the seeds did not present greenish coloring on the endosperm. In the concentrations of 2.5% for 6 hours, 5% and 6% for 2 h and 3 h, intense coloration was observed, making seed evaluation difficult. Seeking to adjust the LERCAF? test methodology, a second experiment was conducted, using a lot of coffee seeds of cultivar Red Catuai IAC 99. This experiment quantified the sodium hypochlorite solution?s content of active chloride and, subsequently evaluated the efficiency of the test, using active chloride concentrations of 1%, 2%, 3%, 4% and 5% and the periods of 1,2,3,4 and 5 hours, at 30 oC. The isoenzymatic profile for enzymes Esterase (EST), Malate Dihydrogenase (MDH), Superoxide Dismutase (SOD), Catalase (CAT) and Alcohol Dehydrogenase (ADH) was also evaluated. The LERCAF? test allows the determination of physiological potential of the coffee seeds, when using quantified sodium hypochlorite solution, by the treatments in which the seeds are immersed in solution with 2% active chloride content for the period of 5 hours, and 3% active chloride for the period of 3 hours, at 30 oC. The coffee seeds submitted to the LERCAF? test presented alterations in the activity of enzymes EST, MDH, SOD, CAT and ADH, being that the activation of deactivation of these enzymatic systems vary with the concentration and time of immersion in active chloride solution.