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21	Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1 Zuo, Shusheng January 2005 (has links) The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. The human red blood cell membranes were stripped to become vesicles with low-ionic alkaline solution in the presence or absence of dithioerithritol. The supernatant of partially solubilized membrane vesicles provided approximately 65% of the vesicle proteins. GLUT1 purified from this supernatant showed a little high-affinity cytochalasin B binding activity. On the other hand, the vesicles stripped with dithioerythritol provided mostly monomeric GLUT1 and those without dithioerythritol provided monomeric and oligomeric GLUT1. MALDI-ToF-MS detected variant GLUT1 fragments between the two preparations. Residual endogenous phospholipids per GLUT1 also showed difference. However, the equilibrium exchange of glucose was retained for both GLUT1 preparations. Cytochalasin B-binding activity of GLUT1 in streptoavidin-biotin-immobilized red blood cells showed that both dissociation constant and binding sites per GLUT1 fell between those of wheat germ lectin-immobilized red blood cells with or without polylysine coating, which indicated the switching of two cytochalasin B-binding states of GLUT1. It is concluded that GLUT1 in red blood cells contains approximately two equal portions, monomeric with high-affinity cytochalasin B-binding activity and oligomeric without high-affinity cytochalasin B-binding activity. In the partial solubilization of the membrane vesicles, GLUT1 which does not have high-affinity cytochalasin B-binding activity is pooled. This might provide a resolution to select oligomerically and functionally different GLUT1 for crystallization. In addition a modified micro-Bradford assay with CaPE precipitation was developed to achieve a routine quantitation method for membrane proteins and the effects of cholesterol and PEG(5000)-DSPE on reconstituted GLUT1 were preliminarily determined. Biochemistry Cholesterol Cytochalasin B Glucose GLUT1 Hummel and Dreyer analysis Immobilization PEG(5000)-DSPE Biomembrane Proteoliposome Sulfhydryl affinity chromatography The modified micro-Bradford CaPE assay MALDI-ToF-MS Biokemi Biochemistry Biokemi
22	The Aesthetic Experience and Artful Public Administration Piccorelli, Justin Thomas 25 August 2014 (has links) No description available. Public Administration Philosophy Aesthetics Phenomenology Aesthetic Experience Administrative Discretion Public Administration Role of the Expert Art of Administration Art Artful Aesthetic Judgment Hannah Arendt Camilla Stivers Merleau-Ponty Ralph Hummel Philosophy of Aesthetics
23	Managing strawberry pollination with wild bees and honey bees: Facilitation or competition by mass-flowering resources? Bänsch, Svenja 05 February 2019 (has links) No description available. 630 Hummel Honigbiene Bienen Solitärbienen Bestäubung Erdbeeren Raps Massentrachten Agrarökologie Landschaftsanalysen Molekularbiologie Bienentanz Schwänzeltanz Pollenanalyse pollination Agroecology bees solitary bees social bees strawberry mass-flowering crops ecology landscape pollen metabarcoding next generation sequencing waggle dance honey bee bumble bee oilseed rape Land- und Forstwirtschaft (PPN621302791)

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