Microbial contamination of drinking water poses an important health risk which causes severe illnesses and epidemics. In order to improve surface and drinking water quality, the understanding of fecal pathogen contamination processes including their prevention and control needs to be enhanced. The watershed model soil water assessment tool (SWAT) is commonly used to simulate the complex hydrological, meteorological, erosion, land management and pollution processes within river basins. In recent years, it has been increasingly applied to simulate microbial contamination transport at the watershed scale. SWAT is used in this study to simulate Escherichia coli (E.coli) and fecal coliform densities for the agriculturally dominated Payne River Basin in Ontario, Canada. Unprecedented extensive monitoring data that consist of 30 years of daily hydrological data and 5 years of bi-weekly nutrient data have been used to calibrate and validate the presented model here. The calibration and validation of the streamflow and nutrients indicate that the model represent these processes well. The model performs well for periods of lower E. coli and fecal coliform loadings. On the other hand, frequency and magnitude of higher microbial loads are not always accurately represented by the model.

Den snabba utvecklingen och urbaniseringen i stora delar av Kina har ett flertal konsekvenser för miljön. Yangtzedeltats ytvatten är till stor del eutrofierade, delvis p.g.a. diffusa förluster från jordbruket. I denna studie har kväve- och, till viss del, fosforflöden och förluster från två odlingsrutor i ett intensivt odlat grönsaksfält i ett tätortsnära område i Nanjing, med hög tillförsel av organiskt gödsel, undersökts med hjälp av den fältskaliga simuleringsmodellen GLEAMS. GLEAMS parametriserades och kalibrerades mot mätvärden av jordens vatten- och kväveinnehåll. Ett scenario med minskad kvävetillförsel simulerades sedan. Simuleringen av vattenhalten i de olika horisonterna var inte utmärkt. Den simulerade mängden mineralkväve i marken var avsevärt lägre än den uppmätta. Detta kan bero på en felaktig simulering av mineraliseringen av organiskt kväve eller en för långsam nedbrytning av gödsel. Det är också möjligt att felen i vattensimuleringarna bidrog till underskattningen av mängden mineralkväve i marken. Simuleringarna på de båda odlingsrutorna gav liknande resultat, förutom att ruta B hade 20% större förluster av N via simulerad erosion och läckage. För fortsatt simulering av alternativa odlingsmetoder bör modellens parametrisering förbättras, särskilt vad avser parametrar kopplade till gödselns mineralisering. / Many parts of China are going through a rapid development and urbanization resulting in various environmental impairities. The Yangtze Delta Region surface water bodies are affected by eutrophication, partly caused by diffuse losses from agriculture. In this study, nitrogen, and to some extent also phosphorus, flows and losses from two plots in an intensively cultivated vegetable field in a peri-urban area of Nanjing, with a high input of organic fertilizer, were analysed by the use of the field-scale simulation model GLEAMS. The GLEAMS model was parameterized and calibrated against measurements of soil water and nitrogen content in two plots. A scenario with a reduced input of nitrogen was then simulated. The resemblance between simulated and measured water content in the different soil layers was quite poor. The simulated inorganic nitrogen content in the soil was significantly lower than the measured during great parts of the simulation period. This could be due to an inappropriate simulation of the mineralization of organic N under these conditions, or an underestimated decomposition rate of manure. It is also possible that the poor water simulations contributed to the underestimated inorganic N content in the soil. There were similar results for the two plots, except for an unexplained 20% increase in leaching and erosion losses of N in Plot B. For simulation of scenarios to find best management practices, the model parameterization should be further refined.

The general objective of the research effort reported in this thesis was to develop the knowledge required to optimize the design and operation of solid and semi-solid manure handling and land application equipment. Selected physical and rheological properties of manure products deemed to have an influence on the performances of manure handling and land application equipment were measured and general trends were identified among the measured properties. Relationships were also established between the measured properties and the type of manure as well as its total solids concentration. Field experiments were carried out to evaluate the effects of selected mechanical configurations, operating parameters and product properties on the discharge of manure spreaders. The influence of the type of conveying system (scraper conveyor and system of four augers) and the velocity at which it is operated, the geometry of the holding system and the position of a flow-control gate were all included in the analysis. The discharge rates of the machines as well as the specific energy required by the unloading operations were measured. A numerical modeling method called discrete element method (DEM) was used to create virtual manure, a numerical model of the real product. The measured physical and flow properties were used to develop and validate the virtual manure models. It was found that manure products could successfully be represented in a DE framework and that several parameters defining the contact constitutive model in the DEM had an influence on the behaviour of the virtual products. The DEM was then used to study machine-product interactions taking place in handling and land application equipment. Results from field experiments carried out using various land application equipment were used in the development and validation of the interaction models. The predicted flow rates and power requirements were in good agreement with measured data. The results obtained allowed for a better understanding of the flow of manure products in manure handling and land application equipment. It was found that the constitutive model used for the product influenced the results of the machine-product interactions models. A precision banded applicator under development at the University of Saskatchewan was also modeled. The discharge rate of this equipment is influenced by a number of parameters. The predicted mass distribution across the width of the banded applicator was well correlated to the experimental results. The models developed in this thesis have the potential to become powerful engineering tools for the design of improved machines for the handling and land application of solid and semi-solid manure.

Development of the swine and cattle industries has led to an increase of manure application to agricultural lands in Saskatchewan. Studies have been conducted to determine the nutrient benefits of swine manure application. However, a need was also identified for information on the effects of manure application on soil physical and chemical properties. The objective of this study was to examine the effect of repeated applications of manure on soil physical and chemical properties and to relate those effects to early plant growth and development.<p>Four experimental sites were used, representing the Dark Brown (Plenty), Brown (Riverhurst irrigated), Black (Dixon) and Gray (Melfort) Soil Zones of Saskatchewan, where liquid swine manure had been applied for four to seven years. At each site, treatments were 1) a control treatment, 2) a nitrogen based agronomic rate of manure application, 3) a high rate of manure application (2-4x the agronomic rate) and 4) a urea fertilizer treatment. At the Dixon site, the same two manure treatments with cattle manure were also examined.<p>Soil strength, as determined by penetration resistance measurements and barley (<i>Hordeum vulgare</i>) emergence were measured at two experimental sites (swine and cattle manure trials at Dixon, SK) in a field study. Penetration resistance was measured at 5, 10, 15 and 20 cm depths, 20, 39 and 123 days after seeding using a recording cone penetrograph. Twenty days after seeding, there were no significant differences among treatments at the 10, 15 and 20 cm depths. But, at the 5 cm depth, the control treatment had soil strength 0.11 MPa lower than the two manure rates. The manure treatments were not significantly different from the urea treatment. Thirty-nine days after seeding, the soil strength of the low rate manure treatment was 1.1 MPa greater than the control at the 10 cm depth, but not significantly different from the urea treatment. One hundred and twenty three days after seeding, the control treatment had greater soil strength than the high rate of manure at 5 and 10 cm depths by 0.28 and 0.71 MPa respectively. At the 20 cm depth, the high rate of manure had the greatest soil strength. Barley emergence on the two manured treatments did not differ significantly from the control. Aggregate size was measured in field samples collected from all sites. Aggregate size for the manured treatments did not differ from the control at any site.<p>Soil crust strength, flax emergence, infiltration rate, salinity, sodicity, coefficient of linear extensibility (COLE) and modulus of rupture were measured under controlled conditions in intact cores of soil removed from all five experimental sites. All soils were treated with a simulated rainfall from a Guelph Rainfall Simulator II. Following the simulated rainfall, crust strength was measured with a hand-held penetrometer. Soil crust strength was measured daily for 10 days as the cores dried. Repeated applications of liquid swine manure at either low or high rates decreased soil strength in the Plenty, Riverhurst and Melfort soils, and increased soil strength in the Dixon soil. Repeated applications of liquid swine manure at low rates caused flax emergence to decrease for the Riverhurst soil compared to its control and had no significant effect at the other sites. There were no notable differences in infiltration rates among treatments. Repeated applications of liquid swine manure caused salinity (EC) to increase slightly for the Plenty and Riverhurst soils, and sodicity (ESP) to increase slightly for the Melfort and Dixon soils relative to their control. The COLE and modulus of rupture measurements indicated no significant effects and were inconclusive due to difficulties in measurement. <p>None of the properties measured in any of the treatments exceeded threshold values for soil productivity, or where plant injury might be considered an issue. It is concluded that repeated (four to seven) annual applications of liquid swine or cattle manure would not cause any large alterations in soil strength, aggregation, infiltration, salinity, or sodicity that would affect early plant growth and development. This was supported by field and lab measurements of emergence that showed limited effect.

Overwintering of cows is a major cost in a cow-calf production system on the prairies. Winter feeding hay and straw directly on pasture is a potentially more efficient and economical system compared to conventional drylot feeding in a yard. The objectives of the research described in this thesis were to compare winter feeding cattle directly on pasture to traditional drylot over-wintering of cattle and the associated mechanized spreading of manure on pasture. This trial compared the effects of winter feeding systems on pasture nutrient distribution, nutrient recovery in soil and forage, pasture forage response, cattle performance, and economics. <p>The experiment was conducted at Lanigan, SK, on an old russian wildrye grass pasture. Pasture nutrient levels and distribution were measured before and after winter feeding, as well as forage yield, and cattle weight and condition. Nutrient capture and cycling was assessed along with the economics of the different systems. In the pasture fed systems, cattle were fed by either bale processing or bale grazing methods over the winter of 2003-2004. Cattle concentration was 2080 cow-days ha-1, with the cattle in the field for 130 d. In the intensive system used for comparison, cattle were fed in a drylot and 67 tonnes ha-1 of raw manure or 22 tonnes ha-1 of compost was mechanically spread on the pasture in the fall of 2003.<p>Soil inorganic nitrogen (N) levels (0-15 cm) measured in the spring where the cattle were winter fed on pasture were 3 to 4 times the unfertilized, unmanured control treatment, with a mean gain of 117 kg N ha-1. Soil inorganic N was not significantly elevated where manure or compost had been spread by machine. Soil extractable potassium (K) was doubled on the winter feeding sites, with a mean gain of 1209 kg K ha-1. Soil extractable K did not increase where manure or compost had been spread mechanically. Soil distribution patterns of both nutrients were highly uneven following pasture feeding, with levels of inorganic soil N ranging from 12 to 626 kg ha-1 and extractable soil K ranging from 718 to 6326 kg ha-1. Additional nutrients in surface residue from uneaten feed, bedding, and manure were also heavy and variable following pasture feeding. Greater retention of N and K from urine added directly to the soil in the field in the bale grazing and bale processing systems compared to the drylot system is believed to be responsible for high soil available N and K levels compared to manure hauled from the drylot into the field.<p>Soil extractable phosphorus (P) levels (0-15 cm) were measured in the fall of 2005. The compost treatment had the largest increase at 2.6 times the control, an additional 46 kg ha-1. Mean soil P levels did not increase significantly where the cattle were wintered.<p> Over 18 months and 3 harvests, forage dry matter yields where the cattle were fed on pasture were 3 to 5 times the control where the cattle were fed on the pasture, and 1.4 to 1.7 times the control where raw manure or compost was mechanically spread. Also, protein content of the forage was increased to a greater extent in the in-field feeding compared to hauled raw manure or compost, reflecting a greater conservation of N.<p>The gain of N in the forage over 18 months on the winter feeding sites was 200 kg ha-1 of N, almost double what was measured in soil inorganic forms. Fourteen kg ha-1 of P was also recovered. This represented 34% of original feed N and 22% of original feed P that was imported into the field. Recovery of nutrients applied in the raw manure and compost sites was much lower, with only 7% recovery of N and 4% recovery of P in the forage. This was calculated to be 1% of original feed N and 3% of original feed P.<p>The system by which the cattle were overwintered had little influence on cattle weight and condition. All systems performed favorably in maintaining body weight and condition over the winter. Some slight advantages in cattle weight gain and condition were found on the winter feeding systems compared to the in-yard drylot that appeared to be related to slightly increased feed intake.<p>Economic calculations favored winter feeding directly on the pasture by 25% over the drylot systems when the feed value of additional pasture growth over 18 months was included and by 56% when the value of additional soil nutrients was factored in. Feed costs were similar between the systems but pasture feeding had savings in machinery use, fuel consumption and manure handling costs, and gains in pasture productivity.<p>Systems that winter fed cattle directly on pasture provided gains in nutrient cycling efficiencies, pasture growth, and economic savings compared to drylot feeding systems, while maintaining similar cattle growth and condition.

On the Canadian prairies, organic production generally includes the use of annual green manure (GrM) crops, which are terminated using tillage to add nutrients and organic matter to the soil. However, in a GrM plough-down year, farmers face loss of income. As an alternative to growing traditional GrM crops, legumes can be grown alone or intercropped with cereals and harvested as green feed forage (GF) for use on-farm or for sale to other producers without depleting soil nitrogen (N) for the subsequent crop. We hypothesized that the GF system would have similar biomass, and N yield, and ultimately would return N into the soil. Furthermore, by intercropping a legume with a cereal, biological N2-fixation will be enhanced in the legume.<p> Field experiments, conducted over two years, were established at Vonda and Delisle, Saskatchewan, Canada. The experiment was conducted using a randomized complete block design (RCBD) with 16 treatments and four replicates in which field pea (<i>Pisum sativum</i> cv 40-10 silage pea), oat (<i>Avena sativa</i> L.cv AC Morgan), and triticale (X <i>Triticosecale</i> Wittmack cv Pika) were grown alone or in combination and managed as GrM or GF. Wheat and tillage fallow served as cropped and uncropped controls, respectively. The tillage fallow-control system was tilled twice in the growing season using a small tractor disc. The intercropped oat was seeded at three densities (50, 100, and 150 plants m-2) to determine whether increasing cereal density stimulated N2-fixation in the field pea.<p> The GrM system was sampled and incorporated (when the field pea was at full bloom) two weeks earlier than the GF system. Consequently, at both sites, all treatments in the GF system consistently yielded more dry matter and accumulated more N than treatments in the GrM system. At the Delisle site, where percent nitrogen derived from the atmosphere (%Ndfa) was compared, increasing cereal density did not increase N2-fixation in both management systems. However, pea in the GF system accumulated more than twice the amount of N (kg ha-1) from fixation as compared to pea in the GrM system, presumably because of the longer growth period.<p> Wheat grown following the GrM treatments produced more biomass and accumulated more N than wheat following the GF treatments. Wheat grown after the monoculture field pea as a GrM had greater yield than all treatments. As well, the GrM system returned more N to the soil than did the GF system. The extra two weeks of growth in the GF system resulted in the extraction of significant amounts of nutrients and probably moisture from the soil, which adversely affected yield and nutrient composition of the following wheat crop.<p> Although organic farmers may lose income in the plough-down year, on a longterm soil sustainability basis, the GrM system is a better option than the GF system as it returns nutrients to the soil, thus providing improved plant biomass, and N accumulation of subsequent crops. However, organic farmers growing GF for hay may benefit from the increased productivity of this system on a short-term basis. Thus, farmers pursuing GF options may need to adopt other means of sustaining soil productivity on a longer term. The tilled fallow-control system resulted in high amounts of biomass and N accumulation by the subsequent wheat crop, probably due to the fact that there were no nutrients taken up in the previous year and moisture was conserved in these treatments. However, this system may have less long-term benefits compared to the GrM regime, as no nutrients are returned through ploughing down a crop.

Manure-based biomass (MBB) has the potential to be a source of green energy at large coal-fired power plants and on smaller-scale combustion systems at or near confined animal feeding operations. Although MBB is a low quality fuel with an inferior heat value compared to coal and other fossil fuels, the concentration of it at large animal feeding operations can make it a viable source of fuel. Mathematical models were developed to portray the economics of co-firing and reburning coal with MBB. A base case run of the co-fire model in which a 95:5 blend of coal to low-ash MBB was burned at an existing 300-MWe coal-fired power plant was found to have an overall net present cost of $22.6 million. The most significant cost that hindered the profitability of the co-fire project was the cost of operating gas boilers for biomass dryers that were required to reduce the MBB's moisture content before transportation and combustion. However, a higher dollar value on avoided nonrenewable CO2 emissions could overrule exorbitant costs of drying and transporting the MBB to power plants. A CO2 value of $17/metric ton was found to be enough for the MBB co-fire project to reach an economic break-even point. Reburning coal with MBB to reduce NOx emissions can theoretically be more profitable than a co-fire project, due to the value of avoided NOx emissions. However, the issue of finding enough suitable low-ash biomass becomes problematic for reburn systems since the reburn fuel must supply 10 to 25% of the power plant?s heat rate in order to achieve the desired NOx level. A NOx emission value over $2500/metric ton would justify installing a MBB reburn system. A base case run of a mathematical model describing a small-scale, on-the-farm MBB combustion system that can completely incinerate high-moisture (over 90%) manure biomass was developed and completed. If all of the energy or steam produced by the MBB combustion system were to bring revenue to the animal feeding operation either by avoided fueling costs or by sales, the conceptualized MBB combustion system has the potential to be a profitable venture.

Anaerobic digestion (AD) is an effective way to convert animal manures into profitable byproducts as well as to reduce the pollution of water, air, and soil caused by these wastes. Conventional high-rate anaerobic reactors cannot effectively process high-solids containing animal manures. The two-phase configuration for AD has several advantages over conventional one-phase processes such as increased stability of the process, smaller and cost efficient process configurations, etc. This study investigated the two-phase AD of dairy manure with particular emphasis on the effects of solids retention time (SRT), organic loading rate (OLR) and pH on anaerobic acidification of unscreened dairy manure / the effects of temperature on biogas production and the comparison of one-phase and two-phase system performance of AD. The results revealed that pre-acidification of dairy manure in daily-fed continuously-mixed reactors with no recycle led to substantial volatile fatty acids production. The optimum operational conditions for anaerobic acidification were determined as SRT and OLR of 2 days and 15 g VS/L.day. The pH control at a range of 5.0-5.5 was not found to be necessary for optimum acidification. Molecular analysis indicated that acidogenic bacteria population increased whilst the aerobic bacteria population decreased as time passed in acidogenic phase. The effect of temperature was clearly observed on biogas production efficiency. Two-phase configuration was determined more efficient than one-phase system. The biogas production in two-phase system was calculated to be 41% higher than that of the one-phase for the same OLR of 3.5 g VS/L.day. This translates into significant performance improvement and reduced volume requirement. This finding represents a further step in the achievement of wider use of simple anaerobic reactor configurations in rural areas.

The main objective of this study was to investigate the removal and recovery of nutrients from anaerobic digestion residues of poultry manure through struvite (MgNH4PO4.6H2O, MAP) precipitation. To this purpose, three sets of laboratory experiments were conducted. In the first set, separate and co-digestion of poultry manure and sewage sludge were studied in laboratory-scale mesophilic anaerobic batch reactors and subsequent struvite precipitation experiments were conducted with the reactors effluents. The effects of important parameters on struvite precipitation were investigated and it is illustrated that up to 89 % of NH4-N, 84 % PO4-P and 42 % COD removals were possible. In the second set, the effluents of a full-scale co-digestion plant utilizing poultry manure and maize silage were subjected to struvite precipitation experiments. Acidic phosphorus-dissolution process was successfully applied to the solid phase effluents to obtain phosphorus-enriched solution. By the addition of external Mg and P more than 90% of NH4-N and PO4-P were recovered from phosphorus-enriched solution, whereas the addition of only Mg led to partial recovery of NH4-N. In the third set, the effluents of a full-scale poultry manure digester were subjected to struvite precipitation experiments. The findings illustrated that Ca has inhibitory effects on the struvite reaction and lead to formation of hydroxlyapatite and amorphous calcium phosphates together with struvite. Based on the results obtained in this study, it is postulated that, struvite precipitation is a viable option for the recovery of the nutrients in the anaerobically digested poultry manure. This study illustrated that, poultry manure, if managed properly, could meet one-fourth of Turkey&amp / #8217 / s domestic fertilizer demand.

Concentrated animal feeding operations (CAFOs) have caused water quality concerns in many rural watersheds, sometimes forcing the State of Texas to conduct Total Maximum Daily Load (TMDL) assessments of stream nutrients such as nitrogen (N) and phosphorus (P). One suggested Best Management Practice (BMP) is the export of phosphorus (P) through turfgrass sod produced with composted dairy manure from an impaired rural watershed to an urban watershed. The manure-grown sod releases P slowly and would not require additional P fertilizer for up to 20 years in the receiving watershed. This would eliminate P application to the sod and improve the water quality of urban streams. The Soil and Water Assessment Tool (SWAT) was used to model a typical suburban watershed that would receive the transplanted sod. The objective of the modeling was to determine the water quality changes due to the import of sod transplanted from turf fields and grown with composted dairy manure. The SWAT model was calibrated to simulate historical flow and sediment and nutrient loading to Mary's Creek. The total P stream loading to Mary's Creek was lower when manure-grown sod was imported instead of commercial sod grown with inorganic fertilizers. Yet, flow, sediment yield, and total N yield increased equally for both cases at the watershed outlet. The SWAT simulations indicate that a turfgrass BMP can be used effectively to import manure P into an urban watershed and reduce in-stream P levels when compared to sod grown with inorganic fertilizers.