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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Reavalia??o dos caracteres da placa peritrem?tica e fest?es para a diagnose de Anocentor nitens (Neumann, 1897), na regi?o Sudeste / Reevaluation of peritremal plate characters and festoons for the diagnosis of Anocentor nitens (Neumann, 1897), in the Southeast region

GAZ?TA, Gilberto Salles 19 July 1993 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-04-10T18:37:45Z No. of bitstreams: 1 1993 - Gilberto Salles Gaz?ta.pdf: 930210 bytes, checksum: 0c425531817e903d01966c5e960cb65b (MD5) / Made available in DSpace on 2018-04-10T18:37:45Z (GMT). No. of bitstreams: 1 1993 - Gilberto Salles Gaz?ta.pdf: 930210 bytes, checksum: 0c425531817e903d01966c5e960cb65b (MD5) Previous issue date: 1993-07-19 / CNPq / Two hundred and four females and one hundred and twenty males of Anocentor nitens (Neumann, 1897), obtained of equines from 12 districts in the Southern part of Brazil were analyzed morphologically concerning its peritremal plate and festoons, that are taxonomics parameters utilized for diagnosis of this species. The number of peritremal plate should variation with predominant asymmetry. Variations observed in the peritremal plate of males and females of A. nitens suggest the formation of cline in Minas Gerais State going to Rio de Janeiro State and S?o Paulo State. The number of festoons in males and females of the species analyzed was equal to the one characterized in the diagnosis of A. nitens / Duzentos e quatro f?meas e cento e vinte machos de Anocentor nitens (Neumann, 1897), oriundos de equ?deos localizados em 12 munic?pios da regi?o Sudeste, foram analisados morfologicamente quanto aos caracteres placa peritrem?ticas e fest?es, par?metros taxon?micos utilizados na diagnose desta esp?cie. As varia??es ocorridas nas Placas Peritrem?tica de macho e f?meas de A. nitens, sugerem a forma??o de ?cline? a partir do Estado de Minas Gerais, em dire??o ao Estado do Rio de Janeiro e ao Estado de S?o Paulo. O n?mero de fest?es dos esp?cimes analisados foi igual, em machos e f?meas, ?quele caracterizados na diagnose de A. nitens.
2

Biodiversidade de helmintos de Scomber colias Gmelin, 1789 (Scombridae) da costa do Rio de Janeiro / Helminth biodiversity of Scomber colias Gmelin, 1789 (Scombridae) from the coast of Rio de Janeiro

MORENO, Aline Braga 25 February 2015 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-10T19:41:52Z No. of bitstreams: 1 2015 - Aline Braga Moreno.pdf: 3503961 bytes, checksum: 9ee745976fac8dfd56f36e7a0d024468 (MD5) / Made available in DSpace on 2018-05-10T19:41:52Z (GMT). No. of bitstreams: 1 2015 - Aline Braga Moreno.pdf: 3503961 bytes, checksum: 9ee745976fac8dfd56f36e7a0d024468 (MD5) Previous issue date: 2015-02-25 / CAPES / FAPERJ / Instituto Oswaldo Cruz / Previous studies on the helminth fauna of scombrid fish from the Brazilian coast included reports on Scomber japonicus Houttuyn, 1782 and Scomber colias Gmelin, 1789. Nowadays its considered that only S. colias occurs in the Atlantic Ocean, Mediterranean Sea and Black Sea, forming different populations. To assess and revise the parasite biodiversity of S. colias off the coast of Rio de Janeiro State, 69 fish from Public Markets of S?o Pedro da Aldeia and Serop?dica were collected and analyzed, from March 2013 to November 2014. Fish were examined and all organs were individualized in Petri dishes containing saline solution 0.7% to be analyzed under the stereo microscope. The parasites were fixed in 70% etanol, AFA or 4% formalin. The Nematoda were cleared and examined in temporary mounts with glycerin and Monogenea, Trematoda and Cestoda were stained with Semichon's acid carmine or Gomori's trichrome and examined as permanent mounts in Canada balsam. The taxonomic identification was made by measuring with the ocular micrometer, by illustrations using a camera clara, and by studies on scanning electron microscopy and molecular techniques. The prevalence, intensity amplitude, mean abundance and standard deviation were calculated per each parasite species. Parasite community study was made at the infracomunity level using the data of 50 fish collected in a single sample from S?o Pedro da Aldeia, which represents a component community. The mean richness, mean total abundance and the mean of Berger-Parker dominance index, mean Bray-Curtis similarity and the mean of Brillouin diversity index of the infracommunities were also calculated. Additionally, using the SIMPER procedure, were also identified the species that contributed most to the similarity between the infracommunities. We collected 578 parasites belonging to 9 taxa: Monogenea (Grubea cochlear and Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis and Allonematobothrioides scombri - new host record and new geographical distribution), Cestoda (Scolex pleuronectis) and Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larvae). Raphidascaris sp. was the most prevalent (64%), followed by K. scombri (60%) and A. laguncula (38%). The most abundant species was K. scombri (2.30% ? 3.73). The mean infracommunity richness was 2.14 ? 1.09 with a total mean abundance of 7.08 ? 7.24. The mean Berger-Parker dominance index was 0.67 ? 0.27, K.scombri, Raphidascaris sp. and A.laguncula being the most dominant species. The Bray-Curtis similarity index between infracommunities was relatively low (32.78 ? 26.20) and Raphidascaris sp. and Hysterothylacium sp. were contributing most to the similarity. / Os trabalhos pr?vios sobre a helmintofauna de peixes escombr?deos da costa brasileira inclu?ram refer?ncias em Scomber japonicus Houttuyn, 1782 e Scomber colias Gmelin, 1789. Hoje se considera que apenas S. colias ocorre no Oceano Atl?ntico, Mar Mediterr?neo e Mar Negro, onde forma popula??es distintas. Com o objetivo de avaliar e revisar a biodiversidade parasit?ria de S. colias, do litoral do Estado do Rio de Janeiro, foram coletados e analisados 69 peixes provenientes de Mercados P?blicos dos Munic?pios de S?o Pedro da Aldeia e Serop?dica, no per?odo de Mar?o de 2013 a Novembro de 2014. Os peixes foram necropsiados e todos os ?rg?os foram individualizados em placas de Petri com solu??o salina 0,7% para serem examinados no microsc?pio estereosc?pico. Os parasitos foram fixados em ?lcool a 70%, AFA ou formalina a 4%. Os Nematoda foram diafanizados e examinados em l?minas tempor?rias com glicerina e os Monogenea, Trematoda e Cestoda corados em Carmim de Semichon ou Tricr?mico de Gomori e examinados em l?minas permanentes montadas em B?lsamo do Canad?. A identifica??o taxon?mica foi feita atrav?s de medidas com aux?lio de ocular microm?trica, desenhos em c?mara clara, estudos por microscopia eletr?nica de varredura e t?cnicas moleculares. Foram calculadas a preval?ncia, amplitude de intensidade, abund?ncia m?dia e desvio padr?o para cada esp?cie de parasito. Os estudos de comunidades se realizaram a n?vel de infracomunidade com os dados de 50 peixes coletados em uma ?nica amostra em S?o Pedro de Aldeia, que representa uma comunidade componente. Foram tamb?m calculadas a riqueza m?dia, a abund?ncia total m?dia, ?ndice m?dio de domin?ncia de Berger-Parker e os ?ndices m?dios de similaridade de Bray-Curtis e de diversidade de Brioullin. Adicionalmente, utilizando o procedimento SIMPER, foram avaliadas as esp?cies que mais contribu?ram para a similaridade entre as infracomunidades. Foram coletados 578 parasitos, pertencentes a 9 taxa: Monogenea (Grubea cochlear e Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis e Allonematobothrioides scombri ? novo hospedeiro e nova distribui??o geogr?fica), Cestoda (Scolex pleuronectis) e Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larva). Raphidascaris sp. apresentou maior preval?ncia (64%), seguido por K. scombri (60%) e A. laguncula (38%). A esp?cie mais abundante foi K. scombri (2,30% ? 3,73). A riqueza m?dia de esp?cies a n?vel de infracomunidade foi de 2,14?1,09 com uma abund?ncia total de 7,08?7,24. As infracomunidades apresentaram um ?ndice de domin?ncia de Berger-Parker de 0,67?0,27 sendo as esp?cies mais dominantes K.scombri, Raphidascaris sp. e A.laguncula. O ?ndice de similaridade de Bray-Curtis entre as infracomunidades foi relativamente baixo (32,78?26,20) e as esp?cies Raphidascaris sp. e Hysterothylacium sp. foram as que mais contribu?ram para a similaridade.
3

Avalia??o micol?gica e micotoxicol?gica de ra??es fornecidas na dieta de til?pias em criat?rios

Barbosa, Tatiana Salom?o 09 February 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-07-29T12:59:33Z No. of bitstreams: 1 2011 - Tatiana Salom?o Barbosa.pdf: 562866 bytes, checksum: 6cdcd52bb7ab78c9cb4b4a47f918a85c (MD5) / Made available in DSpace on 2016-07-29T12:59:33Z (GMT). No. of bitstreams: 1 2011 - Tatiana Salom?o Barbosa.pdf: 562866 bytes, checksum: 6cdcd52bb7ab78c9cb4b4a47f918a85c (MD5) Previous issue date: 2011-02-09 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior- CAPES. / BARBOSA, Tatiana Salom?o. Mycological and mycotoxicological evaluation of feed supplied in the diet of tilapias in breeding. 2011. 37 p. Dissertation (Master?s Degree in Science and Technology of Food). Technology Institute, Federal Rural University of Rio de Janeiro, Serop?dica, RJ, 2011. Pisciculture is a specific branch of aquaculture aimed at raising fish in captivity. For its great advantages tilapia stand out as the queens of modern pisciculture and its cultivation has been pointed out by experts as a promising activity in the world and especially in Brazil due to its potential hydrological, climatic conditions and excellent satisfactory production of grains used in manufacturing ration. It is known these grains are highly susceptible to fungal contamination with possible formation of mycotoxins, and this constitutes a major problem worldwide. Among the genera evolved in mycotoxins production is Aspergillus, Penicillium e Fusarium. The most prevalent mycotoxins are aflatoxins, ochratoxin A, fumonisins, zearalenone and trichothecenes. These are substances capable of affecting the productivity with serious economic losses and risks to animal and human health. Then, this study aimed to determine the water activity and the total contaminant mycoflora, to establish the occurrence of species potentially mycotoxin-producing belonging to the genera Aspergillus, Penicillium and Fusarium, to determine the presence of aflatoxin B1, ochratoxin A, fumonisin B1 and to characterize mycotoxins profile of toxigenic strains isolated from feed supplied in the diet of tilapia in piscicultures. ,Samples (60) were collected from commercial feed directly from the fish-farming properties in Central and South regions of Rio de Janeiro. Water activity was measured . The total mycoflora was determined by serial decimal dilution plate and fungal colonies were identified at genera level. The identification of Aspergillus spp. and Penicillium spp. followed specific taxonomic keys. The profile was conducted to toxigenic strains potentially ochratoxin A and aflatoxin B1 producers isolated from the samples. The determination of the natural incidence of ochratoxin A and aflatoxin B1 was performed by thin layer chromatography and fumonisin B1 was determined by ELISA kits. Data were submitted to analysis of variance (ANOVA) and comparisons of means by Tukey's test (p < 0.05). The average of the aW of the samples was 0.61 ? 0.041 in the Central Region and 0.58 ? 0.060 in the South, both below the value considered optimal for fungal growth and mycotoxin production. Mean levels of fungal contamination were 1.0 x 103 CFU g-1 in the Central and 4.7 x 103 CFU g-1 in the South region, being within the recommended standards for international Good Manufacturing Practice for feed, which is 1.0 x 104 CFU g-1 The most isolated genera were Cladosporium (85%), Aspergillus (68%) and Penicillium (60%). The most frequent species were P. citrinum (31%), A. niger aggregate (21%) and A. flavus (20%). All isolates of A. niger aggregate were negative for the capacity to produce ochratoxin A. Among the A. flavus strains, 16% were positive for the production aflatoxin B1. Concerning the natural incidence of mycotoxins, the samples had detectable levels of fumonisin B1 (98%), aflatoxin B1 (55%) and ochratoxin A (2%). The detection of mycotoxins in almost all the feed samples analyzed highlights the need to implement good agriculture and manufacturing practices, in order to ensure the safety of feed and to reduce the risk of mycotoxicosis occurrence. / BARBOSA, Tatiana Salom?o. Avalia??o micol?gica e micotoxicol?gica de ra??es fornecidas na dieta de til?pias em criat?rios. 2011. 37 p. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos). Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2011. A piscicultura ? um ramo espec?fico da aq?icultura voltada para a cria??o de peixes em cativeiro. Por suas grandes vantagens as til?pias se destacam como as rainhas da piscicultura moderna e seu cultivo vem sendo apontado por especialistas como promissora atividade no mundo e principalmente no Brasil em decorr?ncia do seu potencial hidrogr?fico, condi??es clim?ticas excelentes e produ??o satisfat?ria de gr?os utilizados na fabrica??o de ra??o. Sabese que estes gr?os s?o altamente suscept?veis ? contamina??o f?ngica com poss?vel forma??o de micotoxinas, o constitui um problema de grande import?ncia em n?vel mundial. Dentre os g?neros f?ngicos envolvidos na produ??o de micotoxinas destacam-se o Aspergillus, Penicillium e Fusarium. As micotoxinas de maior preval?ncia s?o as aflatoxinas, ocratoxina A, fumonisinas, zearalenona e tricotecenos. Estas s?o subst?ncias capazes de afetar os par?metros produtivos, com graves perdas econ?micas e riscos para a sa?de animal e humana. Assim, este estudo objetivou determinar a atividade de ?gua e a micobiota total contaminante, estabelecer a ocorr?ncia de esp?cies potencialmente produtoras de micotoxinas pertencentes aos g?neros Aspergillus, Penicillium e Fusarium, determinar a presen?a de aflatoxina B1, ocratoxina A e fumonisina B1 e caracterizar o perfil tox?geno de cepas de esp?cies isoladas de amostras de ra??es fornecidas na alimenta??o de til?pias provenientes de pisciculturas. Para isto, foram coletadas 60 amostras de ra??es comerciais diretamente das propriedades piscicultoras nas regi?es Centro e Sul do Estado do Rio de Janeiro. As amostras tiveram sua atividade de ?gua aferida. Em seguida, a micobiota total foi determinada pelo m?todo de dilui??o decimal seriada em placa. Depois de isoladas, as col?nias foram identificadas em n?vel de g?nero. A identifica??o de Aspergillus spp. e Penicillium spp. seguiu chaves taxon?micas espec?ficas. O perfil tox?geno foi realizado para as cepas potencialmente produtoras de ocratoxina A e de aflatoxina B1 isoladas das amostras. A determina??o da incid?ncia natural destas micotoxinas foi realizada atrav?s de cromatografia em camada delgada. A determina??o da presen?a de fumonisina B1 nas amostras foi feita atrav?s de kits ELISA. Os resultados foram submetidos ? an?lise de vari?ncia (ANOVA) e compara??o de m?dias pelo teste de Tukey (p < 0,05). A m?dia obtida da Aa das amostras foi de 0,61 ? 0,041 na Regi?o Centro e de 0,58 ? 0,060 na Regi?o Sul, ambas abaixo do valor considerado ?timo para o crescimento f?ngico e produ??o de micotoxinas. Os n?veis m?dios de contamina??o f?ngica foram de 1,0 x 103 UFC g-1 no Centro e de 4,7 x 103 UFC g-1 no Sul do Estado, ficando dentro dos padr?es recomendados pelas Boas Pr?ticas de Fabrica??o internacional para a alimenta??o animal, que ? de 1,0 x 104 UFC g-1 de amostra. Os g?neros f?ngicos mais isolados foram Cladosporium (85%), Aspergillus (68%) e Penicillium (60%). As esp?cies mais freq?entes foram P. citrinum (31%), A. niger agregados (21%) e A. flavus (20%). Todas as cepas isoladas de A. niger agregados foram negativas para o teste de capacidade produtora de ocratoxina A. Dentre as cepas de A. flavus isoladas, 16% foram positivas para a produ??o de aflatoxina B1. Quanto ? incid?ncia natural de micotoxinas, 98% das amostras apresentaram n?veis detect?veis de fumonisina B1, 55% de aflatoxina B1 e 2% de ocratoxina A. A detec??o de micotoxinas em quase todas as amostras de ra??o analisadas ressalta a necessidade de implanta??o de boas pr?ticas de agricultura e de fabrica??o, a fim de se garantir um alimento mais seguro e reduzindo os riscos da ocorr?ncia de micotoxicoses.
4

O antagonismo com acetamida em experimentos com ovinos, caprinos e coelhos indica monofluoroacetato como princ?pio t?xico de Pseudocalymma elegans / Antagonism of acetamid in experiments with sheep, goats and rabbits indicates that monofluoroacetate is the toxic principle of Pseudocalymma elegans

Helayel, Michel Jos? Sales Abdalla 16 March 2011 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-19T11:49:34Z No. of bitstreams: 1 2011 - Michel Jos? Sales A. Helayel.pdf: 11350186 bytes, checksum: ab1a4bf6f150ee6e971e58207f995bff (MD5) / Made available in DSpace on 2016-09-19T11:49:34Z (GMT). No. of bitstreams: 1 2011 - Michel Jos? Sales A. Helayel.pdf: 11350186 bytes, checksum: ab1a4bf6f150ee6e971e58207f995bff (MD5) Previous issue date: 2011-03-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This study aimed to evaluate the protective effect of acetamid in experimental poisoning by Pseudocalymma elegans in sheep, goats and rabbits, in order to prove indirectly that monofluoroacetate (MF) is responsible for the clinical signs and death of animals that ingested the plant. Experiments were performed to determine for sheep and goats the lethal dose of P. elegans collected in Rio Bonito, RJ, in different seasons, and to adjust the dose of acetamid to be administered. In the first experiment, four animals received 1.0g/kg of fresh P. elegans, and two others were pretreated with 2.0g/kg of acetamid. None of the animals showed clinical signs or died. Possibly, the plant could be less toxic, since it was collected at the end of the rainy season. In the second experiment, two sheep and two goats received 0.67 and 1.0g/kg of the dried plant, after pretreatment with 2.0 and 3.0g/kg of acetamid, respectively. All animals died, as the administered doses of P. elegans were very high. In the third experiment, two sheep and two goats received 0.333g/kg of dried P. elegans after previous administration of 2.0g/kg of acetamid; a week later, the protocol above was repeated, but without the antidote. In experiments with rabbits, doses of 0.5 and 1.0g/kg of dried P. elegans were given after administration of 3.0g/kg of acetamid; seven days later, the same protocol was repeated, except the administration of acetamide. This procedure, when acetamid was administered before, prevented the appearance of clinical signs and death of sheep, goats and rabbits. But the animals not treated with acetamid showed symptoms of poisoning and died. Clinically, the sheep and goats had tachycardia, engorged jugular vein, positive venous pulse, lateral recumbence, and muscle tremors. In the "dramatic phase?, the animals fell into lateral position, stretched the limbs, were paddling and died within minutes. The rabbits showed apathy, muscle tremors, vocalization and lateral decumbence minutes before death. At postmortem examination, the sheep and goats had engorged jugular veins and atria, dilated Vena cava cranialis and caudalis, as well as pulmonary edema, hepatic congestion and edema of the gallbladder subserosa. In rabbits, the main macroscopic alterations were dilated atria, engorged Vena cava cranialis and caudalis, and congested liver and diaphragm vessels. Histopathology revealed, in two sheep and one goat, vacuolar-hydropic degeneration of the distal convoluted kidney tubules, together with caryopicnosis. In the rabbits, the liver showed severe congestion with numerous shock corpuscles. The experimental results show indirectly that MF is to be held responsible for death of the animals that ingested P. elegans; since "acetate donor" compounds, such as acetamid, are capable to reduce the competitive inhibition of MF for the same active site (Coenzyme A) which prevents the formation of fluorocitrate, its active metabolite, formed in the body through the socalled "lethal synthesis". / O presente trabalho teve como objetivo avaliar o efeito protetor da acetamida nas intoxica??es experimentais por Pseudocalymma elegans (Bignoniaceae) em ovinos, caprinos e coelhos, com a finalidade de comprovar indiretamente que o monofluoroacetato ? respons?vel pela sintomatologia e morte dos animais que ingerem essa planta. Foram realizados experimentos para determinar a dose letal da planta coletada em Rio Bonito, RJ, em diferentes ?pocas do ano para ovinos e caprinos e ajustar a dose de acetamida a ser administrada. No primeiro experimento, dois ovinos e dois caprinos receberam 1,0 g/kg de P. elegans fresca e um animal de cada esp?cie foi tratado previamente com 2,0 g/kg de acetamida. Nenhum animal apresentou altera??es cl?nicas ou morreu. Ao que tudo indica a planta poderia estar menos t?xica, j? que foi coletada no fim da esta??o das ?guas. No segundo experimento, dois ovinos e dois caprinos receberam 0,67 e 1,0 g/kg da planta dessecada, ap?s tratamento pr?vio, com 2,0 e 3,0 g/kg de acetamida, respectivamente. Todos os animais morreram, pois administramos doses muito altas de P. elegans. No terceiro experimento, dois ovinos e dois caprinos receberam, 0,333 g/kg de P. elegans dessecada, ap?s administra??o pr?via de 2,0 g/kg de acetamida. Uma semana depois, o protocolo acima foi repetido, por?m sem o ant?doto. Nos experimentos com coelhos, foram administradas doses de 0,5 e 1,0 g/kg de P. elegans dessecada ap?s a administra??o de 3,0 g/kg de acetamida. Sete dias depois, o mesmo protocolo foi repetido, com exce??o da administra??o de acetamida. Esta, quando administrada previamente, evitou o aparecimento dos sinais cl?nicos e a morte dos ovinos, caprinos e coelhos, j? os animais n?o tratados com acetamida apresentaram sintomatologia e morreram. Clinicamente, os ovinos e caprinos manifestaram taquicardia, jugulares ingurgitadas, pulso venoso positivo, dec?bito esternal e tremores musculares. Na ?fase dram?tica?, os animais ca?am em dec?bito lateral, esticavam os membros, faziam movimentos de pedalagem e morriam em poucos minutos. Nos coelhos observaram-se apatia, tremores musculares, dec?bito lateral e vocaliza??o minutos antes da morte. A avalia??o macrosc?pica revelou, nos ovinos e caprinos, jugulares ingurgitadas, aur?culas, veia cava caudal e cranial dilatadas, al?m de edema pulmonar, congest?o hep?tica e edema na subserosa da ves?cula biliar. Nos coelhos as principais altera??es observadas foram aur?culas dilatadas, veia cava caudal e cranial ingurgitadas, f?gado e vasos do diafragma congestos. O exame histopatol?gico revelou, em dois ovinos e um caprino, degenera??o hidr?pico-vacuolar dos t?bulos urin?feros contornados distais associada ? cariopicnose. Nos coelhos havia congest?o hep?tica acentuada com numerosos corp?sculos de choque. Nossos resultados comprovam, de forma indireta, que o MF ? respons?vel pela morte dos animais que ingerem essa planta, uma vez que compostos ?doadores de acetato? como a acetamida, s?o capazes de reduzir a inibi??o competitiva do MF pelo mesmo s?tio ativo (Coenzima A), o que impede a forma??o do fluorocitrato, seu metab?lito ativo, formado no organismo por meio da denominada ?s?ntese letal?.
5

Efeito de diferentes fontes de sangue sobre a performance reprodutiva de Aedes aegypti L., 1762 (D?ptera: Culicidae) alimentados atrav?s de membrana de silicone / Effect of different blood sources on the reproductive performance of Aedes aegypti L., 1762 (Diptera: Culicidae) fed through silicone membrane device

PINA, Isabelle Garcia 30 September 1997 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-14T17:34:23Z No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) / Made available in DSpace on 2017-02-14T17:34:23Z (GMT). No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) Previous issue date: 1997-09-30 / CNPq / This experimental work with Aedes aegypti L. (1762) was conducted in order to compare the effect of differents blood sources on feeding and egg production of mosquitoes? females. The blood-feeding device consisted of a plastic cage ? used for holding females during blood feeding ? with two lateral windows recovered with thin cloth to allow air exchange during feeding. In the top of the cage, there was an opening where the food?s container, recovered with silicone membrane, was attached. A larger vial filled with water at the initial temperature of 50 ?C was used over the apparatus to keep the food source warm (37,5 ? 0,5%). In each trial, three different treatments were tested: whole blood collected from mouse, bovine and human. To prevent coagulation, a 3,9% solution of sodium citrate was added to the blood. The control group was allowed to feed directly on human volunteers. The experiment was conducted in a climatic chamber, with temperature and humidity controlled at 28?0,5 ?C and 80?5%, respective values and the photoperiod of 12 hours of light and 12 hours of darkness. In each tested group, 30 females and 10 males mosquitoes were used. The females were allowed to feed during a period of 20-30 minutes and after that only the engorged females were transferred to another cages containing the oviposition sites. To evaluate the effect of different sources of blood on A. aegypti, the percentage of engorged females, pre-oviposition time and the number of eggs per engorged female were measured. Highest percentage of engorged females were among mosquitoes fed on human citrated blood (89%) and control (95%). The media oviposition on both treatments were 13,4 eggs for females fed on human citrated blood and 15,3 eggs for females of control group. The lots fed on mice and bovine citrated blood presented the following results respectively: 22,5% of engorged females and 9,2 eggs per female and 25,8% of engorged females and 10,3 eggs per female. / Foi comparado o efeito do sangue de camundongos, bovino e humano sobre o ingurgitamento e oviposi??o do mosquito Aedes aegypti L., 1762 (D?ptera:Culicidae). O aparato para alimenta??o artificial foi constitu?do por gaiolas de pl?stico, com 10 x 6 cm, com orif?cios laterais cobertos com tela de tule, e uma abertura superior, atrav?s da qual foi oferecido o sangue contido em uma c?lula montada em l?mina de microscopia com cola de silicone e membrana de silicone. O sangue foi mantido aquecido a 37,5 ? 0,5 ?C, durante o tempo de alimenta??o que variou de 20 a 30 minutos. O experimento foi conduzido dentro de uma c?mara climatizada, com temperatura de 28 ? 0,5 ?C, umidade relativa de 80 ? 5% e fotoperiodismo de 12 horas di?rias. Um total de 16 grupos de 30 f?meas de A. aegypti, com idades entre 4 a 7 dias, foram pr?-alimentados com solu??o a?ucarada de sacarose. Em cada grupo foram adicionados 10 machos para realiza??o de c?pula. O sangue de cada hospedeiro foi coletado assepticamente e adicionado de citrato de s?dio a 3,9%. O grupo controle foi alimentado diretamente em hospedeiro humano volunt?rio. Ap?s a alimenta??o, as f?meas ingurgitadas foram contadas e colocadas no interior de gaiolas com 50 x 50 x 50 cm, contendo frasco com ?gua limpa para oviposi??o. Foram registrados o n?mero de f?meas alimentadas em cada tratamento, o per?odo de pr?-postura e o n?mero m?dio de ovos por f?mea alimentada. Mosquitos alimentados com sangue de camundongos e de bovino tiveram performances de 22,5 e 25,8% de ingurgitamento e a postura foi em m?dia de 9,2 e 10,3 ovos por f?mea, respectivamente. F?meas alimentadas com sangue humano atrav?s da membrana ou diretamente no hospedeiro humano tiveram comportamentos diferentes com ?ndices de ingurgitamento de 89 e 95% e oviposi??o de, em m?dia, 13,4 e 15,3 ovos por f?mea, respectivamente. N?o houve varia??o no per?odo de pr?-postura (tr?s dias) entre os tratamentos realizados.
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Diagn?stico sorol?gico de Rickettsia spp. e Borrelia spp. em c?es no munic?pio de Serop?dica, RJ / Serological diagnosis of Rickettsia spp. and Borrelia spp. in dogs in the municipality of Serop?dica, RJ

CORDEIRO, Matheus Dias 29 February 2012 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-11T21:16:25Z No. of bitstreams: 1 2012 - Matheus Dias Cordeiro.pdf: 852495 bytes, checksum: c420562c23340ca737374426f8c5e0f7 (MD5) / Made available in DSpace on 2017-04-11T21:16:25Z (GMT). No. of bitstreams: 1 2012 - Matheus Dias Cordeiro.pdf: 852495 bytes, checksum: c420562c23340ca737374426f8c5e0f7 (MD5) Previous issue date: 2012-02-29 / CNPq / Brazilian Spotted Fever and Baggio-Yoshinari Syndrome are emerging diseasest characterized as the only known to tick-borne zoonoses in Brazil. This study aimed to avaluate IgG antibodies against Borrelia burgdorferi and Rickettsia rickettsii in stray and domiciled dogs from Serop?dica-RJ and studying the fauna of ixodida these animals. To investigate the prevalence of antibodies against R. rickettsii and B. burgdorferi was performed to collect blood from 293 dogs in four areas of the city of Serop?dica-RJ. Serum samples were processed by Indirect Immunofluorescence (IFA) against crude antigens of R. rickettsii. The same animals were tested for IgG antibodies against B. burgdorferi strain of North American origin, using the Indirect Enzyme-linked immunosorbent assay (ELISA). To confirm the presence of Borrelia spp., 102 positive samples were tested by Western Blotting (WB). Ticks were also collected from the inspection of the animals. The specimens found were manually removed and stored in polypropylene bottles, and later identified. The association between seropositive animals and variables, were performed using the chi-square and analysis of variance (ANOVA) with significance level of 5%. Of the 283 sera tested by IFA, 23.67% (67/283) showed reactivity against species-specific antigens of R. rickettsii. Thus, we found a prevalence of 21.11% (19/90) in "40 km", 21.84% (19/87) in "Km 49", 25% (8 / 32) in "54 km" and 28.38% (21/74) in UFRRJ. The titles ranged from 1:64 to 1:512. On the other hand, of the 293 animals studied, 154 (52.56%) were positive for homologous antibodies anti-B. burgdorferi. It was founded a prevalence of 43.75% (14/32) in the "54 km", 51.72% (45/87) in the "49 km", 46.67% (42/90) in the "40 km" and 63.1% (53/84) in UFRRJ. It was no significant difference (p<0.05) between the prevalence of antibodies against Borrelia sp. in stray dogs and domiciled. Dogs with access to the street had a frequency against R. rickettsii significantly (p<0.05) greater than animals kept in prison. There were no statistical differences (p>0.05) the relationship between the variables: presence of ticks, animal sex, habits, frequenting pastures and age of the animals with seropositivity observed in either species. Regarding ticks, at least 64.5% (189/293) dogs were infested with ticks at the moment of collection. Only two species of ticks were identified, Rhipicephalus sanguineus and Amblyomma cajennense. The presence of antibodies anti-R. rickettsii and anti-B. burgdorferi in dogs from Serop?dica-RJ is indicative of the presence of rickettsias of the Rock Mountain Spotted Fever Group and spirochetes dogs in this area. / A Febre Maculosa Brasileira e a S?ndrome de Baggio-Yoshinari s?o enfermidades emergentes, caracterizadas como as ?nicas zoonoses conhecidamente transmitidas por carrapatos, no Brasil. O presente estudo teve como objetivo detectar anticorpos da classe IgG contra Rickettsia rickettsii e Borrelia burgdorferi e estudar a fauna de Ixod?deos nesses animais. Para investigar a preval?ncia de anticorpos contra R. rickettsii e B. burgdorferi foi efetuada a coleta de sangue de 293 c?es, em quatro ?reas do munic?pio de Serop?dica-RJ. Os soros obtidos foram processados atrav?s da Rea??o de Imunofluoresc?ncia Indireta (RIFI), contra ant?genos brutos de R. rickettsii. Os mesmos animais foram submetidos ? pesquisa de anticorpos hom?logos da classe IgG contra B. burgdorferi cepa americana G39/40, utilizando Ensaio de Imunoadsor??o Enzim?tico (ELISA) Indireto. Para confirma??o da presen?a de Borrelia spp. no munic?pio, 102 amostras positivas foram testadas pelo Western Blotting (WB). A fauna de ixod?deos foi estudada atrav?s da coleta de carrapatos a partir da inspe??o das regi?es das orelhas, dorso e coxins palmares e plantares dos animais. Os esp?cimes encontrados foram removidos manualmente e acondicionados em frascos de polipropileno, e posteriormente, identificados. O estudo da associa??o entre animais soropositivos e as vari?veis avaliadas, foram realizados por meio do teste de Qui-quadrado e An?lise de Vari?ncia (ANOVA), com n?vel de signific?ncia de 5%. Dos 283 soros testados ? RIFI, 23,67% (67/283) apresentaram reatividade contra ant?genos esp?cie-espec?fico de R. rickettsii, sendo encontrada uma frequ?ncia de 21,11% (19/90) no ?Km 40?, 21,84% (19/87) no ?Km 49?, 25% (8/32) no ?Km 54? e 28,38% (21/74) no campus da UFRRJ. A titula??o variou entre 1:64 ? 1:512. Por outro lado, dos 293 animais estudados, 154 (52,56%) foram positivos para anticorpos hom?logos anti-B. burgdorferi, sendo encontrada uma frequ?ncia de 43,75% (14/32) no ?Km 54?, 51,72% (45/87) no ?Km 49?, 46,67% (42/90) no ?Km 40? e 63,1% (53/84) na UFRRJ. Houve diferen?a significativa (p<0,05) entre as preval?ncias de anticorpos contra Borrelia spp. encontradas em caninos errantes e domiciliados. Os c?es com acesso a rua tiveram uma frequ?ncia de anticorpos contra R. rickettsii significativamente (p<0,05) maior que animais mantidos preso. N?o foram observadas diferen?as estat?sticas (p>0,05) entre a rela??o das vari?veis: presen?a de carrapatos, sexo dos animais, h?bitos de frequentar pastos e idade dos animais com a soropositividade observada em nenhuma das duas esp?cies. Quanto aos carrapatos, 64,5% (189/293) dos c?es estavam infestados por carrapatos no momento da coleta. Apenas duas esp?cies de carrapatos foram identificadas, Rhipicephalus sanguineus e Amblyomma cajennense. A presen?a de anticorpos hom?logos anti-R. rickettsii e anti-B. burgdorferi em caninos de Serop?dica-RJ ? um indicativo da presen?a de rickettsia do grupo da Febre Maculosa e espiroquetas em c?es nesta ?rea.
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Aspectos epidemiol?gicos da Babesia canis vogeli em c?es da Baixada Fluminense, RJ / Aspects of Babesia canis vogeli in dogs from Baixada Fluminense, RJ

VILELA, Joice Aparecida Rezende 30 March 2012 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-05T17:41:38Z No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) / Made available in DSpace on 2017-05-05T17:41:38Z (GMT). No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) Previous issue date: 2012-03-30 / CAPES / The canine babesiosis, is a disease widely distributed in Brazil and worldwide, caused by? protozoa of the genus Babesia?spp. that parasitize erythrocytes and it?s transmitted by ticks? Ixodidae. This study aimed to characterize the epidemiology of canine babesiosis through? questionnaires and serological diagnosis by ELISA, diagnosis of Babesia? spp. infection in? dogs by real?time PCR technique, identify the subspecies of B. canis?by RFLP, characterize? the hematological aspects, diagnosis by direct microscopy, identification of ectoparasites and? diagnosis by real?time PCR, the agent of canine babesiosis in ticks from dogs from rural and? urban areas from Serop?dica city, RJ State. The present study evaluated 311 dogs, 167? (n=53,7%) of rural and 144 (n=46,3%) in urban areas. By ELISA technique, was found 39? dogs (12,54%) serologically positive for Babesia canis, with frequency of seropositivity of? 21,56% ???(n=36/167) in dogs of rural area and 2,08% (n=3/144) in dogs from urban area, and? therefore the rural area, the highest seropositivity (p<0,0001). The variables associated with? seropositivity were: area of residence of the dog, breed, age, presence of ticks, environment of? dog access, contact with other animals, the animal's habits, condition of cleanliness of the? environment, absence of veterinary care and animal wandering. The profile of the owner and? its management were striking features in parasitism by ticks and consequently the frequency? of seropositivity to the agent. The diagnostic by PCR real??time of blood samples from dogs? showed a frequency of 11,90% (n=37/311). The subspecies identified affecting dogs of this? region was B. canis vogeli. Of the total of 311 dogs evaluated, infestations by ectoparasites? were found in 252 (81,03%) dogs at the time of collection. Of these, 70,64% (n=178/252)? were infested with ticks. In relation to the infestation, Rhipicephalus sanguineus?was found in? 68,54% (n=122/178) of the dogs, Amblyomma cajennense?in 11,80% (n=21/178), A. ovale?in? 3,37% (n=6/178) and A. dubitatum? in 0,56% (n=1/178). Nymphs of R. sanguineus? and? Amblyomma sp. were observed in 13,48% (n=24/178) and 5,62% (n=10/178) of dogs infected? with ticks, respectively. The tick species most common in urban and rural areas was R. sanguineus, which was associated with positivity of B. canis?in dogs by molecular method.? The frequency of ticks positive for B. canis?was 5% (n=12/240). The DNA from B. canis?was? detected in nine R. sanguineus?ticks, an A. cajennense?adult and two nymphs of Amblyomma? sp. The main hematological changes in infected dogs were: anemia, thrombocytopenia and? hemoglobinemia. The study concluded that canine babesiosis caused by B. canis vogeli?occurs? in rural and urban areas from the Serop?dica being of great importance its occurrence in the? cities of the Rio de Janeiro State, which have characteristics similar expansion of urbanization? to rural areas with little distinction between the two areas and points to the importance of? diagnosis and identification of the pathogen and vectors in dogs, in order to know the? epidemiological conditions for the adoption of appropriate preventive measures, because in? addition to affect the animal health, many of these vectors are capable to transmit pathogens? to humans.? / A babesiose canina, patologia de ampla distribui??o no Brasil e no mundo, ? causada por? protozo?rios do g?nero Babesia? spp., que parasitam hem?cias e s?o transmitidos por? carrapatos Ixodidae. O presente trabalho objetivou determinar a epidemiologia da babesiose? canina atrav?s de aplica??o de question?rios e diagn?stico por ELISA, diagn?stico por? microscopia direta, diagn?stico de Babesia canis? por meio da PCR em tempo real,? identifica??o das subesp?cies de B. canis? por meio da RFLP, caracteriza??o das principais? altera??es hematol?gicas, identifica??o dos ectoparasitos e diagn?stico atrav?s da PCR em? tempo real do agente da babesiose canina em carrapatos de c?es do munic?pio de Serop?dica,? Baixada Fluminense ??RJ. No presente estudo foram avaliados 311 c?es, sendo 167 (n=53,7%)? de ?rea rural e 144 (n=46,3%) de ?rea urbana. Atrav?s do teste de ELISA, encontrou?se um? total de 39 c?es (12,54%) sorologicamente positivos para Babesia canis, com frequ?ncia de? 21,56% (n=36/167) nos c?es de ?rea rural e 2,08% (n=3/144) nos c?es de ?rea urbana, sendo,? portanto a ?rea rural, a de maior soropositividade (p<0,0001). As vari?veis que apresentaram? associa??o com a soropositividade foram: ?rea de domic?lio do c?o, defini??o racial, idade,? infesta??o por carrapatos, locais de acesso do c?o, contato com outros animais, h?bito do? animal, ambiente do c?o, aus?ncia de limpeza do ambiente, aus?ncia de assist?ncia? veterin?ria, aus?ncia de abrigo e origem errante. O perfil do propriet?rio e o manejo do c?o? apresentaram caracter?sticas marcantes no parasitismo por carrapatos e consequentemente na? frequ?ncia de soropositividade para o agente. O diagn?stico pela PCR em tempo real de? amostras sangu?neas de c?es revelou uma frequ?ncia de 11,90% (n=37/311). A subesp?cie? identificada acometendo c?es desta regi?o foi B. canis vogeli. Do total de 311 c?es avaliados,? foram observadas infesta??es por ectoparasitos em 252 (81,03%) c?es. Destes, 70,64%? (n=178/252) apresentaram?se infestados por carrapatos. Em rela??o ? infesta??o por? carrapatos, Rhipicephalus sanguineus foi encontrado em 68,54% (n=122/178) dos c?es,? Amblyomma cajennense em 11,80% (n=21/178), A. ovale em 3,37% (n=6/178) e A. dubitatum em 0,56% (n=1/178) dos c?es infestados por carrapatos. Ninfas de R. sanguineus e? Amblyomma sp. foram observadas em 13,48% (n=24/178) e 5,62% (n=10/178) dos c?es? infestados por carrapatos, respectivamente. A esp?cie de carrapato mais frequente nos meios? urbano e rural foi R. sanguineus, que apresentou associa??o com a positividade de B. canis? vogeli?nos c?es pelo m?todo molecular. A frequ?ncia de carrapatos positivos para B. canis foi? de 5% (n=12/240). O DNA de B. canis foi detectado em nove carrapatos adultos de R. sanguineus, em um adulto de A. cajennense? e em duas ninfas de Amblyomma? sp.. As? principais altera??es hematol?gicas nos c?es infectados foram: anemia, hemoglobinemia e? trombocitopenia. O estudo permitiu concluir que a babesiose canina causada por B. canis vogeli?ocorre nas ?reas rurais e urbanas do munic?pio estudado sendo de grande import?ncia a? sua ocorr?ncia nos munic?pios da Baixada Fluminense do Estado do Rio de Janeiro, os quais? apresentam caracter?sticas semelhantes de expans?o da urbaniza??o para o meio rural com? pequena distin??o entre as duas ?reas e aponta para a import?ncia do diagn?stico do pat?geno? e identifica??o de vetores nos c?es, visando o conhecimento das condi??es epidemiol?gicas? para a ado??o de medidas profil?ticas adequadas, pois al?m de afetarem a sanidade animal? muitos destes vetores s?o capazes de veicularem pat?genos transmiss?veis ao homem.??
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Parabasal?deos de animais dom?sticos: morfologia, diagn?stico e algumas considera??es epidemiol?gicas / Parabasalids of domestic animals: morphology, diagnosis and some epidemiological considerations

SANTOS, Caroline Spitz dos 26 February 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-22T18:03:31Z No. of bitstreams: 1 2016 - Caroline Spitz dos Santos.pdf: 4739307 bytes, checksum: 0073345f08ba619e059be0cc4a39b481 (MD5) / Made available in DSpace on 2017-06-22T18:03:31Z (GMT). No. of bitstreams: 1 2016 - Caroline Spitz dos Santos.pdf: 4739307 bytes, checksum: 0073345f08ba619e059be0cc4a39b481 (MD5) Previous issue date: 2016-02-26 / CAPES / The study on parabasalids in companion animals is gaining more attention for its association with diarrhea. The flagellate Pentatrichomonas hominis has been reported in association with domestic cats since the early 20th century. As a eurixenic parasite has been described in several hosts, including humans, dogs, bovines, rats, and a variety of wild animals. Another parabasalid with great importance in livestock, Tritrichomonas foetus has been also described in cats, pigs, and humans. These observations raise questions about the zoonotic potential of both species and their host specificity. This study aimed to diagnose parabasalid species found in cats using morphological and molecular analysis. Therefore, this study was divided into two parts to assess two different cat populations. In the first part, 41 animals in a trial cattery were evaluated. Twenty-six percent of the animals (11) were positive for P. hominis, at both techniques as fresh examination and culture. The DNA was extracted from the samples in culture and rRNA genes were amplified by polymerase chain reaction (PCR) using universal primers (TFR1 and 2) and other two specific species for T. foetus (TFR3 and 4) and P. hominis (TH3 and 5). Morphological analysis of trophozoites revealed the presence of five previous plagues core round and axostyle tapering uniform, characteristic way of P. hominis. The results of morphology were confirmed by molecular study. The sequencing of the isolates revealed a sequence with 100% similarity to P. hominis isolated from cats and dogs deposited in Genbank. This is the first study in Brazil with pointed out the presence of Parabasalids in cats by using morphological and molecular data and it is the one in the literature where P. hominis was isolated. In the second part of this study, 77 samples of feces from cats from the clinical care of HVPA-UFRRJ were examined. Only four of 77 samples tested were positive. Morphological analysis showed predominantly pear-shaped protozoa with three previous scourges, elongated nucleus and ax?stilo abruptly ending in characteristic fillet in T. fetus. In scanning electron microscopy and transmission, were visualized the identifying characters were similar to those previously reported for T. foetus. Molecular analysis confirmed the morphological diagnosis in the organism from four samples showed a sequence with 99.7 to 100% of similarity. It was deposited in Genbank as T. foetus. Despite the morphological analysis have recognized only T. foetus in the four samples examined, three of them were also positive for P. hominis in molecular analysis used as a differential diagnosis using species-specific primers (TH3 and TH5). The molecular analysis was used as a confirmatory tool for the presence of only one species present in evaluated feces. This demonstrated that not only T. foetus was identified in this study, but a co-infection by P. hominis cats could be considered. This indication was only confirmed as a diagnostic techniques when the morphological analysis and molecular biology were used to confirm both species. / O estudo sobre parabasal?deos em animais de companhia vem ganhando cada vez mais aten??o por sua associa??o a quadros de diarreia. O flagelado Pentatrichomonas hominis foi relatado em associa??o com gatos dom?sticos desde o in?cio do s?culo 20. Por ser um parasito eurix?nico, j? foi descrito em diversos hospedeiros, incluindo os seres humanos, c?es, bovinos, ratos e uma variedade de animais selvagens. Assim tamb?m outra esp?cie de parabasal?deo com grande import?ncia na pecu?ria, Tritrichomonas foetus j? foi descrito em gatos, su?nos, e em humanos tamb?m. Tais observa??es levantam d?vidas sobre o potencial zoon?tico de ambas as esp?cies e sua inespecificidade hospedeira. Este estudo teve por objetivos diagnosticar esp?cies de parabasal?deos encontrados em gatos utilizando de an?lise morfol?gica e molecular. Para tanto, este estudo foi dividido em duas partes para avaliar duas popula??es felinas distintas. Na primeira parte, 41 animais de um gatil de experimenta??o foram avaliados. Vinte e seis por cento dos animais (11) foram positivos para P. hominis, tanto no exame a fresco quanto na cultura. O DNA foi extra?do das amostras em cultura e os genes de rRNA foram amplificados por rea??o em cadeia da polimerase (PCR), utilizando iniciadores universais (TFR1 e 2) e outros dois esp?cies espec?ficos para T. foetus (TFR3 e 4) e P. hominis (TH3 e 5). A an?lise morfol?gica dos trofozo?tos revelou a presen?a de cinco flagelos anteriores, n?cleo redondo e ax?stilo afunilando de maneira uniforme, caracter?stico de P. hominis. Os resultados da morfologia foram confirmados pelo estudo molecular. O sequenciamento dos isolados revelou 100% de similaridade de sequ?ncia com P. hominis isolado de gato e de c?o depositados no Genbank. Este ? o primeiro estudo realizado no Brasil sobre a presen?a de parabasal?deos em gatos utilizando dados morfol?gicos e moleculares e o ?nico na literatura onde somente P. hominis foi isolado. Na segunda parte deste estudo, 77 amostras de fezes de gatos provenientes do atendimento cl?nico do HVPA-UFRRJ foram examinadas. Somente quatro amostras apresentaram positivas. A an?lise morfol?gica demonstrou protozo?rios predominantemente piriformes com tres flagelos anteriores, n?cleo alongado e ax?stilo terminando bruscamente em filete caracter?stico de T. foetus. Na microscopia eletr?nica de varredura e de transmiss?o, foram visualizados caracteres de identifica??o semelhantes aos descritos na literatura para T. foetus. ? an?lise molecular, confirmou o diagn?stico morfol?gico nas quatro amostras, e no sequenciamento apresentaram 99,7-100% de similaridade com sequencias de T. foetus depositadas no Genbank. Apesar da an?lise morfol?gica ter reconhecido somente T. foetus nas quatro amostras, tr?s delas foram positivas tamb?m para P. hominis na an?lise molecular utilizada como diagn?stico diferencial utilizando iniciadores esp?cie-espec?fica (TH3 e TH5). O estudo molecular foi utilizado como ferramenta confirmat?ria da presen?a de somente uma esp?cie presente nas fezes avaliadas. Isso demonstra que n?o s? T. foetus foi identificado pelo presente estudo, como tamb?m a coinfec??o por P. hominis em felinos. Estas informa??es s? foram confirmadas quando se utilizou as t?cnicas de diagn?stico em conjunto como an?lise morfol?gica simples e biologia molecular.
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Avalia??o da efici?ncia do extrato de ra?z Dahlstedti pentaphylla (Leguminosae, Papilionoidae, Millettiedae) sobre Boophilus microplus (CANESTRINI, 1887) na Regi?o do Vale do Para?ba - S?o Paulo, Brasil / Evaluate the efficiency of root extracts of Dahlstedt pentaphylla (Leguminosae, Papilionoidae, Millettiedae) about Boophilus microplus (Canestrini, 1887) in Para?ba Valley ? S?o Paulo, Brasil

PEREIRA, Jos? Roberto 25 May 2004 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-23T17:23:05Z No. of bitstreams: 1 2004 - Jos? Roberto Pererira.pdf: 1063290 bytes, checksum: ac86f4bd5958ba416f5739fd8e9c3de5 (MD5) / Made available in DSpace on 2017-06-23T17:23:05Z (GMT). No. of bitstreams: 1 2004 - Jos? Roberto Pererira.pdf: 1063290 bytes, checksum: ac86f4bd5958ba416f5739fd8e9c3de5 (MD5) Previous issue date: 2004-05-25 / Laboratory and field trials were performed to evaluate the efficiency of Timb? root extract (Dahlstedtia pentaphylla) (Taub). Burk. (Leguminosae, Papilionoidae, Millettiedae) on samples of Boophilus microplus (Canestrini, 1887) in bovines of the Para?ba Valley region ? S?o Paulo, Brasil. The ?in vitro? trials were carried out using immersion of engorged females, and ?in vivo? on bovines in the field by means of artificial infestation. The standard dilution was made from one part of root powder and three parts of ethanol this was considered the 100% solution. To determine the more efficient solvent to extract rotenone from the root, laboratory trials were conducted on B. microplus engorged females with in three solvents: water, ethanol a.p. and acetone a.p. The laboratory trials permitted the calculation of lethal doses at 90% (LD90) and 50% (LD50) for larvae between seventh and fourtheenth days of application and efficient concentrations at 90% (EC90) and 50% (EC50) on strain local and Mozo with B. microplus engorged females. For the larvae of the local strain (Para?ba Valley Regional Hub) LD50 calculated was 1:31,37 mL and LD90 was 1:85,24 mL. The EC90 for engorged females, of the same strain was of 1:10,19 mL and the EC50 1:34,94 mL. For the engorged females on the hypersersitive strain Mozo the EC90 was 1:23,91 mL and the EC50 1:60,46 mL. The mortality of engorged females in relation to the different kinds of solvents, was analyzed. In the field, the best results (76,10% of control) were obtained three days after application of the product extracted in ethanol, in 1:10, on animals. Then gradually between the seventh and fourteenth days the products lost efficiency, there was no significant difference between treatments and the control group after 21 days. / Foram realizados testes laboratoriais e de campo para avaliar a efici?ncia do extrato de ra?zes da planta Dahlstedtia pentaphylla (Taub.) Burk., (Leguminosae, Papilionoideae, Millettiae) sobre amostras de Boophilus microplus ( Canestrini, 1887 ) de bovinos da regi?o do Vale do Para?ba e da cepa sens?vel Mozo. Os testes foram efetuados ?in vitro?, pela t?cnica de imers?o de tel?ginas e ?in vivo?, sobre bovinos no campo, ap?s infesta??o artificial. As dilui??es foram obtidas a partir da extra??o de roten?ides utilizando?se uma parte de p? das ra?zes da planta para tr?s de etanol, sendo considerada padr?o 100%. Visando determinar o solvente mais eficiente para a extra??o de roten?ides das ra?zes, conduziram-se testes laboratoriais sobre tele?ginas de B. microplus em tr?s solventes: ?gua, etanol p.a e acetona p.a. Os testes laboratoriais permitiram calcular a Dose Letal 90% (DL90) e DL50 para larvas com idade entre sete e 21 dias e a Concentra??o Eficaz 90% (CE90) e CE50 sobre tele?ginas de B. microplus das cepas local e Mozo. Para as larvas da cepa local (Polo Regional do Vale do Para?ba) a DL50 calculada foi de 1: 231,37 mL e DL90 1: 85,24 mL. A CE90 para as tele?ginas, da mesma cepa, foi de 1:10,19 mL e a CE50 1: 34,94 mL. Para tele?ginas da cepa sens?vel Mozo a CE90 foi de 1: 23,91 mL e a CE50, 1: 60,46 mL. Analisou-se tamb?m a mortalidade das tele?ginas frente aos diferentes solventes. No campo os melhores resultados obtidos (76,10% de controle) foram obtidos tr?s dias ap?s a aplica??o do produto extra?do em etanol, na dilui??o 1: 10, sobre os animais. A partir da? gradualmente nos dias sete e quatorze os produtos foram perdendo efici?ncia, n?o apresentando mais diferen?a significativa entre os tratamentos e o grupo controle no dia +21.
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Estudos experimentais de Plasmodium juxtanucleare Versiani e Gomes, 1941 em Gallus gallus utilizando as t?cnicas microsc?picas e moleculares com ?nfase na padroniza??o de PCR em tempo real para o diagn?stico / Experimental studies Plasmodium juxtanucleare Versiani and Gomes, 1941 Gallus gallus using microscopic and molecular techniques with emphasis on standardization of real-time PCR for the diagnosis

VILELA, Thamyris Sampaio 27 February 2015 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-26T17:32:22Z No. of bitstreams: 1 2015 - Thamyris Sampaio Vilela.pdf: 3001387 bytes, checksum: d945149445f8ee2765222f5475c7eaa2 (MD5) / Made available in DSpace on 2017-06-26T17:32:22Z (GMT). No. of bitstreams: 1 2015 - Thamyris Sampaio Vilela.pdf: 3001387 bytes, checksum: d945149445f8ee2765222f5475c7eaa2 (MD5) Previous issue date: 2015-02-27 / CAPES / CNPq / FAPERJ / This study aimed to develop a real-time PCR (qPCR) assay for diagnosis of Plasmodium spp. using as target the 18S rDNA and Cyt b genes. A range of 101 blood samples were collected from Gallus gallus in poultry rustic breeds of in Rio de Janeiro, Brazil. The collected bloods were used to prepare blood smears and to extract the deoxyribonucleic acid (DNA) of these samples. There with, molecular protocols were tested, such as the already published conventional PCR (cPCR) and nested PCR (nPCR), designed for Plasmodium spp. In this study two qPCR protocols were developed using primers targeting the Cyt b and 18S rDNA genes. The qPCR detection limit for both genes was 10 copies of the target DNA, which were higher than the detection limit observed in nPCR and cPCR. In qPCR, 69.30% (n = 70/101) samples were positive targeting 18Sr DNA gene and 59.40% (n = 60/101) samples were positive targeting Cyt b gene. In nPCR and cPCR, 54.45% (n = 55/101) and 52.47% (n= 53/101) samples were positive, respectively. In blood smear microscopy, 31 (30.69%) samples were positive. There was no disagreement between the results (p > 0.05) of qPCR for 18Sr DNA and Cyt b genes. Additionally, qPCR was more sensitive than the other techniques discussed, mostly related to blood smear microscopy (p < 0.05). Therefore, the two qPCR developed in the study showed more sensitivity than other techniques and enabled the detection of Plasmodium spp. in poultry even in low parasitemia. / Este estudo teve como objetivo desenvolver um ensaio de PCR em tempo real (qPCR) para o diagn?stico de Plasmodium spp. utilizando os genes 18S rDNA e cyt b. Foram coletadas 101 amostras de sangue de aves da esp?cie Gallus gallus de cria??o r?stica ou org?nica no munic?pio de Serop?dica, Rio de Janeiro. Os sangues coletados foram utilizados na prepara??o de esfrega?os de sangue e para extra??o do ?cido desoxirribonucl?ico (DNA) destas amostras. Protocolos de ensaios moleculares foram testados, tal como o PCR convencional (cPCR), nestedPCR (nPCR) e qPCR para Plasmodium spp. Neste estudo dois protocolos de qPCR foram desenvolvidos utilizando oligoiniciadores desenhados com alvo no citocromo b e 18S rDNA. O limite de detec??o para os dois genes qPCR foi de 10 c?pias do alvo de DNA, que foram maiores do que o limite de detec??o observado em nPCR e cPCR. Em qPCR, 69,30% (n = 70/101) amostras foram positivas com alvo no gene 18SrDNA e 59,40%(n = 60/101) amostras positivas com alvo no gene cyt b. Em nPCR e cPCR, 54,45% (n = 55/101) e 52,47% (n = 53/101) amostras foram positivas, respectivamente. Em microscopia de esfrega?o de sangue, 31 (30,69%) amostras foram positivas. N?o houve discord?ncia entre os resultados (p> 0,05) de qPCR para os genes 18SrDNA e cyt b.. Al?m disso, qPCR foi mais sens?vel do que as outras t?cnicas discutidas, principalmente relacionado com a microscopia ?ptica (p <0,05). Portanto, os dois ensaios de qPCR desenvolvidos neste estudo mostraram mais sensibilidade do que outras t?cnicas e permitiu a detec??o de Plasmodium spp. em aves de cria??o r?stica mesmo com baixa parasitemia.

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