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Newly recognized rat parvoviruses : characterization and diagnostic assay development /Wan, Zhuohua, January 2001 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 2001. / "May 2001." Typescript. Vita. Includes bibliographical references (leaves 85-89). Also available on the Internet.
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Newly recognized rat parvoviruses characterization and diagnostic assay development /Wan, Zhuohua, January 2001 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 85-89). Also available on the Internet.
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Differentiating between living and non-living prokaryotic cells : development, evaluation, and application of a modified vital stain and probe method (mVSP)Howard-Jones, Michelle Hope 08 1900 (has links)
No description available.
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I. Development of a new assay and inhibitors for human cystathionine beta-synthase II. Asymmetric catalyst development guided by in situ enzymatic screening (ISES) /Shen, Weijun. January 1900 (has links)
Thesis (Ph.D.)--University of Nebraska-Lincoln, 2007. / Title from title screen (site viewed Aug. 1, 2007). PDF text: 292 p. : ill. (some col.) UMI publication number: AAT 3256642. Includes bibliographical references. Also available in microfilm and microfiche formats.
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Assessment of the effect of dosing regime and cell culture model on micronucleus induction in in vitro genotoxicity test systemsChapman, Katherine Emma January 2015 (has links)
No description available.
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A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeateNordmark, Tor Soren 24 November 1993 (has links)
The traditional filter paper assay for saccharifying cellulase
originally described by M. Mandels et al (1976) has been modified to
make possible low activity determinations of Trichoderma
cellulases. The enzymatic activity appears to decline during a
prolonged incubation period if no precautions have been taken. By
means of adding bovine serum albumin and potassium chloride as
protein stabilizers and sodium azide as an antimicrobial agent filter
paper activities in the range from 0.02 to 0.37 (IUPAC assay, 1987)
can be estimated by extending the incubation time up to 20 hours. Filter paper activity values obtained by this method may be
compared to those obtained by the IUPAC assay by using a conversion
factor from 1.4 to 1.7.
Acid whey and sweet whey permeate have been investigated as
media for growth and metabolite production by Trichoderma reesei
QM 9414 using shake flask cultures and spore inocula. In the case of
acid whey the mycelial growth after 2 weeks is 13 mg dry weight
/ml substrate. The specific growth rate is 0.29/day. The fungus
appears to metabolize the whey protein the first 2 weeks. The
alkalinity of acid whey rises continuously over a three week period
up to a pH of 8.5. In the case of whey permeate the maximal mycelial
weight gain is 4.4 mg/ml which appears after 8 days. A rise in net
soluble protein level comes after 3-5 days and reaches a maximum
value of 0.23 mg/ml after 2 weeks. The pH of whey permeate rises
continuously to 7.5 after 3 days and then slowly declines. The net
production of cellulases is low on both media. Dilution 1:6 of the
acid whey, supplementation with ammonium sulfate and pHadjustments
did not enhance the production of cellulases. Acid
whey supports a significant growth and sweet whey permeate shows
potential for extracellular protein production.
A literature review surveys the composition and uses of acid
whey, environmental aspects of whey wastes, the fungus
Trichoderma reesei, the mode of action of the Trichoderma reesei
cellulase system and the structure of cellulose in cotton and wood. / Graduation date: 1994
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Rapid assay for Bacillus proteinases in raw milk as detected by a simple casein denaturation methodFeijoo, Sergio C. 09 January 1991 (has links)
A casein agar diffusion method was developed to detect and quantify pertinent
levels of proteinases produced in raw milk supplies by heat resistant Bacillus
sporeformers. In order to optimize the required heat treatment conditions of raw milk
samples, trials that involved a combination of different temperatures and times were
evaluated. A heat treatment of 75°C for 20 min was the most effective for recovering the
highest number of surviving spores. A sporulation broth containing five different
minerals and supplemented with 0.2% nonfat dry milk was used to maximize spore
production in all heat-treated samples.
A β-casein based assay detected proteinase activity from raw milk samples that
ranged from 0.093 to 4.034 units/mg which corresponded to zones of β-casein
precipitation in the β-casein agar of 5.0 and 15.0 mm respectively, and was compared to
Protease Type VIII (from B. licheniformis). This assay correlated well with the
fluorescein isothiocyanate casein-labeled assay (FITC), R=0.995 (Protease Type VIII). Proteases of Bacillus origin such as Protease Type IX, X, XV and XXXI were also
evaluated but were rejected in favor of a broader range of activity expressed by Protease
Type VIII. For an initial set of 370 raw milk samples, no quality deterioration, such as
coagulation or bitter taste was observed in heat-treated (75°C for 20 min) and incubated
samples (7.2°C for 10 days). However, during the winter season, 18 of 75 incubated
samples (7.2°C for 10 days) tasted slightly bitter and exhibited a slight degree of casein
precipitation. One sample coagulated but exhibited no proteinase activity on the β-casein
agar gel, hence it was considered a false negative. The positive results for proteinase
activity from raw Grade A samples tested by the β-casein agar diffusion method did not
correlate either with fresh spore counts (R=0.21) or post-heat treatment incubation counts
(R=0.03) or with psychrotrophic sporeformer counts (R=0.06).
The β-casein agar diffusion method is simple, rapid and sensitive to Bacillus spp.
proteinases, but was unreliable in projecting results related to the psychrotrophic
sporeformer count. Consequently, further research is required to establish optimum
conditions (time and/or temperature) and inoculum volumes into sporulation broth for
attainment of a more positive correlation between β-casein agar precipitation zones and
psychrotrophic sporeformer populations of either raw or processed milk samples. / Graduation date: 1991
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Modified limiting dilution analysis : a mathematical model with biological interpretationMaier, Stefan H. 04 April 1994 (has links)
A mathematical model of Limiting Dilution Analysis for two limiting
parameters is presented and investigated. Limiting Dilution Analysis is a microbiological
cell assay developed for immunological application. In the given
case we deal with the interaction between B lymphocytes, macrophage derived
factor and T-independent antigens. The state of the art is that quantitative
statements are only possible if one cell type (in general the B cells) is limiting
and all others are in excess present.
The basis for this thesis is a set of experiments in which B cells and
macrophage derived factor are limiting and all other involved cells and factors
are in saturating amounts present. It is shown that so far presented suggestions
on modeling Limiting Dilution Analysis for two limiting cell-types are not
suitable for this problem. Further, a mathematical model based on data is
presented and interpreted in immunological terms with the help of a set of
partial differential equations. The basis for the interpretation of the model
are changes in affinity and saturation effects, both not incorporated in the so
far presented models of the assay. In particular the relevance of mathematical
interpretation of this process for the identification of new concepts as the
saturation effects is stressed.
The model of partial differential equations is highly non-linear but offers
the possibility of interpreting the highly interrelated processes apart from each
other. / Graduation date: 1994
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Development of a field-based assay for rapid detection of enterohemorrhagic Escherichia coli (EHEC)Willford, John Daniel. January 2008 (has links)
Thesis (Ph.D.)--University of Wyoming, 2008. / Title from PDF title page (viewed on August 5, 2009). Includes bibliographical references (p. 123-138).
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Development of an in vitro assay for MMP cleavageWu, Wing-kei, Ricky., 胡永基. January 2005 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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