abstract / Chemistry / Doctoral / Doctor of Philosophy

Wong, Cheuk Hon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 69-75). / Abstracts in English and Chinese. / Thesis Committee --- p.ii / Statement --- p.iii / Abstract --- p.iv / 摘要 --- p.V / Acknowledgements --- p.vi / General Abbreviations --- p.viii / Abbreviations of Chemicals --- p.X / List of Figures --- p.xi / List of Tables --- p.xii / Table of Contents --- p.xiii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Food insecurity and abiotic stress --- p.1 / Chapter 1.2 --- Overview of the molecular basis of salt tolerance in plants --- p.4 / Chapter 1.2.1 --- Stress perception --- p.4 / Chapter 1.2.2 --- Signal transduction --- p.5 / Chapter 1.2.2.1 --- ABA dependent pathway --- p.6 / Chapter 1.2.2.2 --- ABA independent pathway --- p.8 / Chapter 1.2.2.3 --- The SOS pathway --- p.9 / Chapter 1.2.3 --- Abiotic stress tolerance mechanisms --- p.9 / Chapter 1.2.3.1 --- Ion transporters --- p.10 / Chapter 1.2.3.2 --- Osmolytes --- p.10 / Chapter 1.2.3.3 --- Antioxidants and ROS scavenging genes --- p.11 / Chapter 1.2.3.4 --- Late Embryogenesis Abundant proteins --- p.12 / Chapter 1.2.3.5 --- "Heat shock factor, heat shock element and heat shock protein" --- p.12 / Chapter 1.2.3.5.1 --- Hsp families --- p.14 / Chapter 1.2.3.5.2 --- Hsp network --- p.18 / Chapter 1.3 --- Pervious studies on GmDNJl --- p.19 / Chapter 1.4 --- "Hypothesis, significances and objectives of this project" --- p.19 / Chapter Chapter 2 --- Materials and Methods --- p.21 / Chapter 2.1 --- Materials --- p.21 / Chapter 2.1.1 --- "Plants, strains, and vectors" --- p.21 / Chapter 2.1.2 --- Chemicals and reagents --- p.24 / Chapter 2.1.3 --- Commercial kits --- p.25 / Chapter 2.1.4 --- Primers --- p.26 / Chapter 2.1.5 --- Equipment and facilities --- p.27 / Chapter 2.1.6 --- Sequencing --- p.27 / Chapter 2.1.7 --- "Buffer, solution and medium" --- p.27 / Chapter 2.1.8 --- Software --- p.28 / Chapter 2.2 --- Methods --- p.29 / Chapter 2.2.1 --- Growth conditions of bacterial and yeast cultures --- p.29 / Chapter 2.2.2 --- Growth conditions and multiple abiotic stress treatments of Glycine max for GmDNJl expression study --- p.29 / Chapter 2.2.2.1 --- "RNA extraction, cDNA synthesis of real-time PCR" --- p.30 / Chapter 2.2.3 --- Growth conditions and multiple abiotic stress treatments of Arabidopsis thaliana for root length essay --- p.30 / Chapter 2.2.3.1 --- Surface sterilization of Arabidopsis thaliana seeds --- p.30 / Chapter 2.2.3.2 --- Abiotic stress treatments of Arabidopsis thaliana for root length essay --- p.31 / Chapter 2.2.4 --- Multiple abiotic stress treatments of Tobacco Bright Yellow-2 (BY2) cell for cell viability assay --- p.31 / Chapter 2.2.4.1 --- Construction of transgenic GmDNJl BY2 cell lines --- p.31 / Chapter 2.2.4.2 --- DNA extraction of transgenic GmDNJl BY2 cell lines by CTAB method --- p.32 / Chapter 2.2.4.3 --- PCR screening of transgenic GmDNJl BY2 cell lines --- p.33 / Chapter 2.2.4.4 --- Gene expression checking of GmDNJl in transgenic BY2 cell lines by real-time PCR --- p.34 / Chapter 2.2.4.4.1 --- RNA extraction of BY2 cell --- p.35 / Chapter 2.2.4.4.2 --- cDNA synthesis of BY2 cell RNA --- p.35 / Chapter 2.2.4.4.3 --- Real-time PCR ofBY2 cell cDNA --- p.36 / Chapter 2.2.4.5 --- Multiple abiotic stress treatments of BY2 cell lines for cell viability assay --- p.37 / Chapter 2.2.5 --- Yeast two-hybrid experiment --- p.37 / Chapter 2.2.5.1 --- Subcloning oi GmDNJl into pGBKT7 --- p.37 / Chapter 2.2.5.2 --- Transformation of pGBKT7 into Y187 --- p.38 / Chapter 2.2.5.3 --- Toxicity test of GAL4 GmDNJl fusion protein in Y187 --- p.38 / Chapter 2.2.5.4 --- Western blot analysis to check the expression of GAL4-GmDNJl in Y187 --- p.39 / Chapter 2.2.5.5 --- Screening of GmDNJl interacting protein partners by yeast mating --- p.39 / Chapter 2.2.5.6 --- Identification of positive interaction partners by retrieving plasmid from diploid yeast --- p.40 / Chapter Chapter 3 --- Results --- p.41 / Chapter 3.1 --- "Expression of GmDNJl was induced under dehydration stress, NaHCOs stress, oxidative stress and heat stress" --- p.41 / Chapter 3.2 --- Ectopic expression of GmDNJl in transgenic Arabidopsis does not confer extra tolerance under multiple stresses in root length essay --- p.44 / Chapter 3.3 --- Construction of transgenic GmDNJl BY2 cell --- p.47 / Chapter 3.3.1 --- PCR screening of GmDNJl in transgenic BY2 cell lines --- p.47 / Chapter 3.3.2 --- Gene expression checking of GmDNJl in transgenic BY2 cell lines by real-time PCR --- p.49 / Chapter 3.3.3 --- Ectopic expression of GmDNJl in transgenic BY2 cell lines do not enhance tolerance under multiple stresses in cell viability essay --- p.51 / Chapter 3.4 --- Yeast two-hybrid experiment --- p.53 / Chapter 3.4.1 --- Subcloning of pGBKTl-GmDNJl --- p.54 / Chapter 3.4.2 --- Toxicity test of GAL4 DNA BD-c-Myc-GmDNJ 1 fusion protein inY187 --- p.54 / Chapter 3.4.3 --- Western blotting showing expression of GAL4 DNA BD -c-Myc-GmDNJl in Y187 --- p.55 / Chapter 3.4.4 --- Screening of GmDNJl putative protein interacting partners by yeast mating --- p.56 / Chapter Chapter 4 --- Discussion --- p.60 / Chapter 4.1 --- "Expression of GmDNJl was induced under dehydration stress, NaHCOs stress, oxidative stress and heat stress" --- p.60 / Chapter 4.2 --- Ectopic expression of GmDNJl in transgenic Arabidopsis and BY2 cells did not confer extra tolerance under multiple stresses in root length essay --- p.63 / Chapter 4.3 --- Yeast two-hybrid experiment --- p.64 / Chapter 4.4 --- Putative function of GmDNJl --- p.65 / Chapter 4.5 --- Future Perspectives --- p.66 / Chapter Chapter 5 --- Conclusion --- p.68 / Chapter Chapter 6 --- References --- p.69 / Chapter Appendix I --- Chemical and reagents --- p.76 / Chapter Appendix II --- "Buffer, solution, gel and medium formulation" --- p.78 / Chapter Appendix III --- Supplementary results --- p.81

Thesis (Ph.D.)--Virginia Commonwealth University, 2009. / Prepared for: Dept. of Human Genetics. Title from title-page of electronic thesis. Bibliography: leaves 102-114.

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy

No description available.

Thesis (Doctor of Philosophy in Biomedical Sciences)--Ponce School of Medicine Pontificia Universidad Católica de Puerto Rico, 1994. / Includes bibliographical references (leaves 80-91).

Thesis (M.Med.Sc) - University of Queensland, 1977. / Includes bibliography.

>Magister Scientiae - MSc / Myocardial infarction, commonly known as a heart attack, is a condition where the blood supply to the heart tissue is cut off, starving the tissue from oxygen and nutrient supply, with consequent lethal damage to the heart tissue. This damage is as a result of the death of cardiomyocytes. Numerous studies demonstrated that the death of these cells is as a result of programmed cell death or apoptosis. Heat shock proteins can protect cardiomyocytes against cell death by inhibiting apoptosis. For this reason heat shock responsive proteins are emerging as therapeutic targets to suppress cell death in cardiomyocytes during myocardial infarction. RhoE and TIP41 are also amongst the genes that are upregulated in cardiomyocytes after heat stress. These genes do not encode classical heat shock proteins. The question that arises is whether the induction of RhoE during heat stress in cardiomyocytes has any cytoprotective role. This research project aims to investigate the potential cytoprotective role of RhoE and TIP41 in rat cardiomyocytes. Mutant cell lines that stably over-express RhoE and TIP41 were generated by transfecting H9c2 cells with the pcDNA-3.1-TOPO vector containing these genes. DNA transfections were performed using the metafectene transfection reagent. Over-expression was investigated using Western blot analysis. The mutant cell lines were treated with ceramide and camptothecin for a period of 24 hours and cell viability was assessed by the MTT assay. Two dimensional proteomic analysis was carried out to compare the proteomes of H9c2 and H9c2 cells that over-express RhoE. This research demonstrates that both RhoE and TIP41 are induced in response to heat stress and that the over-expression of RhoE is able to protect H9c2 against camptothecin induced cell death. Furthermore a proteomic 2D analysis demonstrates differential protein expression between H9c2 cells and H9c2 that over-express RhoE. Proteomic analysis demonstrates that the over-expression of RhoE leads to the down-regulation of Rho-GDI α. It can be concluded from this study that the expression of RhoE in response to heat shock is a cytoprotective event. The mechanism of cytoprotection is likely to involve Rho-GDI α.

No description available.

Thesis (Ph. D.)--University of Chicago, Dept. of Pathology, Dec. 2003. / Includes bibliographical references. Also available on the Internet.