Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-25T23:51:26Z No. of bitstreams: 1 TabataLoiseCunhaLima_DISSERT.pdf: 1403767 bytes, checksum: 7424c70f286f04743b45ac7531e6829b (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-28T20:23:59Z (GMT) No. of bitstreams: 1 TabataLoiseCunhaLima_DISSERT.pdf: 1403767 bytes, checksum: 7424c70f286f04743b45ac7531e6829b (MD5) / Made available in DSpace on 2016-04-28T20:23:59Z (GMT). No. of bitstreams: 1 TabataLoiseCunhaLima_DISSERT.pdf: 1403767 bytes, checksum: 7424c70f286f04743b45ac7531e6829b (MD5) Previous issue date: 2015-03-13 / A dengue ? uma doen?a de notifica??o compuls?ria e cerca de 50 a 100 milh?es de casos s?o registrados anualmente. Possui amplo espectro cl?nico e ? transmitida ao homem atrav?s da picada dos mosquitos do g?nero Aedes, tendo como principal vetor a esp?cie Aedes aegypti. O agente etiol?gico da doen?a ? o v?rus dengue (DENV) pertencente ao g?nero Flavivirus, fam?lia Flaviviridae e s?o conhecidos quatro sorotipos antigenicamente distintos (DENV-1, DENV-2, DENV-3 e DENV-4). Atualmente o tratamento da dengue ? apenas de suporte, feito atrav?s de intensa hidrata??o. Ainda n?o existe uma vacina comprovadamente eficaz ou tratamento espec?fico, o estudo de poss?veis antivirais que possam diminuir a viremia no paciente ? de alt?ssima relev?ncia, uma vez que a carga viral ? um dos fatores associado ao aparecimento das formas graves da doen?a (febre hemorr?gica da dengue e s?ndrome do choque da dengue). No presente estudo n?s avaliamos o potencial antiviral de extratos brutos obtidos a partir das folhas das plantas do Nordeste brasileiro Annona muricata (graviola) e Spondias mombin (caj?) contra o DENV-2 em cultura de c?lulas C6/36 e Vero. A avalia??o da a??o dos extratos brutos foi feita por meio da quantifica??o da carga viral atrav?s da PCR em Tempo Real (qRT-PCR) e pela t?cnica de contagem de unidades formadoras de placa (PFU). As concentra??es dos extratos de ambas as plantas utilizadas foram: 0,01, 0,1 e 1mg/mL. As culturas de c?lulas infectadas foram submetidas ao tratamento com os extratos durante os per?odos de 24-168h horas (7 dias). C?lulas Vero tratadas com o extrato da S. mombin n?o apresentaram redu??o na carga viral. Em contrapartida, quando estas c?lulas foram tratadas com o extrato da A. muricata, uma hora ap?s infec??o, observou-se uma redu??o significativa na carga viral nas primeiras horas (24h), quando comparadas com as c?lulas n?o tratadas utilizadas como controle positivo. Ao serem tratadas em intervalos de 24 horas apresentaram uma redu??o na carga viral nos dias subsequentes (at? o s?timo dia). N?o foi observada redu??o na carga viral em c?lulas C6/36 tratadas com ambos os extratos. De acordo com os nossos resultados, o extrato da planta A. muricata possui potencial antiviral promissor contra a infec??o pelo DENV-2 em cultura de c?lulas Vero. / Dengue is a reportable disease and about 50 to 100 million cases are reported annually. It has a wide clinical spectrum and is transmitted to humans through the bite of Aedes mosquitos, the main vector the Aedes aegypti species. The causative agent of disease is dengue virus (DENV) belonging to the genus Flavivirus, family Flaviviridae and are known four antigenically distinct serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). Currently the treatment of dengue is supportive, made by intense hydration. Although there is no proven effective vaccine or specific treatment, the study of potential antiviral drugs that can reduce viremia in patients is very high importance, since the viral load is one of the factors associated with the development of severe forms of the disease (hemorrhagic fever dengue and dengue shock syndrome). In the present study we evaluated the antiviral potential of crude extracts obtained from the leaves of plants in Northeastern Brazil Annona muricata (soursop) and Spondias mombin (caja) against DENV-2 in cultured C6/36 and Vero. The evaluation of the activity of the crude extracts was performed by the quantification of viral load by RT-PCR (qRT-PCR) and counting technique of plaque forming units (PFU). The concentrations of extracts of both plants used were 0.01, 0.1 and 1 mg/mL. The infected cell cultures were subjected to treatment with the extracts during periods of 24-168h hours (7 days). Vero cells treated with the S. mombin extract showed no reduction in viral load. In contrast, when these cells were treated with the extract of A. muricata, one hour after infection, significant reductions in viral load in the first hour was observed (24 h) when compared to untreated cells used as positive control. When they are treated at 24 hour intervals showed a reduction in viral load in subsequent days (until day). There was no reduction in viral load in C6/36 cells treated with both extracts. According to our results, the plant extract has antiviral A. muricata promising potential against infection by DENV-2 in Vero cell culture.

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-10-05T14:40:58Z No. of bitstreams: 1 2005 - Isabel da Concei??o Gama Silva e Lima.pdf: 2535972 bytes, checksum: 3693f1b4ed58c18a135a182df5e7e78d (MD5) / Made available in DSpace on 2016-10-05T14:40:58Z (GMT). No. of bitstreams: 1 2005 - Isabel da Concei??o Gama Silva e Lima.pdf: 2535972 bytes, checksum: 3693f1b4ed58c18a135a182df5e7e78d (MD5) Previous issue date: 2005-12-14 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES / The red mombin tree (Spondias purpurea L.) is originary from Central America and had adapted satisfactorily to the climatic conditions of some South-American countries as Brazil. This tree produces a fruit of good appearance, nutritive quality, and much appreciated aroma, which is consumed as in natura or processed fruit as frozen pulps, juices, candies, nectars, popsicles and ice creams. This evidence shows its increasing commercialization in grocery stores, supermarkets and restaurants of country. However, there are commercial losses, which can be attributed to biologic and phytopatologic causes that indicate alarming numbers to producers. The study of red mombin composition can contribute to popularize the use of this fruit, once that it is unknown to the most of Rio de Janeiro city people. This research had as objective the characterization of the red mombim fruit (Spondias purpurea) produced in West Zone of Rio de Janeiro and the formularization of products made from pulp aiming to expand the increasing of its productive chain. The centesimal composition demonstrated that the fruit fractions with peel present greater fiber contents, therefore, the consumption of the fruit with peel must be stimulated in order to get its benefits. The researched minerals were found in larger concentrations in the fruit fractions with peel, with prominence for iron and zinc contents. However, studies should be made in order to verify the biodisponibility of these minerals. The analytical data also demonstrated that the red mombin had high vitamin C content, which is an important aid in the iron absorption by the organism. The fractions with peel had a more intense color than the pulp, probably because of the amount of received solar radiation. The red mombin had the - criptoxantin xanthophyll as main carotenoid, one of the most important provitamin A compounds. The formulated red mombin products were jellies and fruit pastes with different concentrations of sucrose. The microbiological analysis performed on elaborated products showed that hygienical-sanitary and technological conditions were satisfactory. The sensorial analysis showed that the attributes appearance, color, aroma, texture and flavor got average around seven at nine point scale hedonic, which represents the category ?I like it moderately?. It indicates that exotic flavor of fruit was well accepted by consumers. The percentage of judgers demonstrated that purchase intentions for jelly and red mombin paste was fairly representative, 92 % and 82% respectively. It evidences the possibility to introduce the products made from red mombin to the market, increasing the period of useful life and the added-value of the fruit. / Frut?fera tropical t?pica do continente americano, a serigueleira (Spondias purpurea L.) ? origin?ria da Am?rica Central, tendo se adaptado satisfatoriamente ?s condi??es clim?ticas de alguns pa?ses da Am?rica do Sul, como o Brasil. Esta produz um fruto de boa apar?ncia, qualidade nutritiva, e aroma muito apreciados para o consumo como fruta in natura ou processada, como polpa, sucos, doces, n?ctares, picol?s e sorvetes, sendo evidente a crescente comercializa??o nos mercados, supermercados e restaurantes do Pa?s. Entretanto, existem perdas comerciais, as quais est?o ligadas principalmente a fatores biol?gicos e fitopatol?gicos, que indicam n?meros preocupantes para os produtores. O estudo da composi??o da seriguela pode contribuir para popularizar o uso deste fruto, desconhecido pela maior parte da popula??o da cidade do Rio de Janeiro. A pesquisa teve como objetivo a caracteriza??o qu?mica da fruta Seriguela cultivada na zona oeste do Rio de Janeiro e a formula??o de produtos a partir da polpa do fruto visando expandir o crescimento da sua cadeia produtiva. Os resultados da composi??o centesimal demonstraram que as fra??es com casca cont?m maior teor de fibras, portanto deve-se incentivar o consumo dos frutos com casca que representa h?bito alimentar saud?vel. Os minerais pesquisados foram encontrados em maior concentra??o nas fra??es da fruta com casca, com destaque para os teores de ferro e zinco. Entretanto, devem ser realizados ainda estudos no sentido de verificar a biodisponibididade desses minerais. Os dados anal?ticos demonstraram tamb?m que a seriguela possui alto teor de vitamina C, sendo importante aux?lio na absor??o do ferro pelo organismo. As fra??es com casca possu?am uma colora??o bem mais intensa que a polpa, provavelmente devido ? quantidade de radia??o solar recebida. A seriguela possui a xantofila - criptoxantina, importante por sua atividade pr?-vitam?nica A, como caroten?ide majorit?rio. Os produtos de seriguela formulados foram gel?ias e doces de corte com diferentes concentra??es de sacarose. As an?lises microbiol?gicas realizadas nas gel?ias e nos doces de corte elaborados atestaram que os produtos apresentavam condi??es higi?nico-sanit?rias satisfat?rias. A an?lise sensorial indicou que os atributos apar?ncia, cor, aroma, textura e sabor apresentaram m?dias pr?ximas a sete em uma escala hed?nica de nove pontos, que representa a impress?o ?gostei moderadamente?, indicando que o sabor ex?tico da fruta foi bem recebido pelos consumidores. O percentual de provadores que demonstrou inten??o de compra para gel?ia e doce de corte de seriguela foi bastante representativo, 92 % e 82% respectivamente, evidenciando a possibilidade de introduzir no mercado produtos a base de seriguela, aumentando a vida-de-prateleira e o valor agregado do fruto.