Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-04T19:56:38Z No. of bitstreams: 1 GiocondaDiasRodriguesLeao_TESE.pdf: 44025376 bytes, checksum: 9da025324f9108141027f1f3a1cc3e16 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-05T20:29:30Z (GMT) No. of bitstreams: 1 GiocondaDiasRodriguesLeao_TESE.pdf: 44025376 bytes, checksum: 9da025324f9108141027f1f3a1cc3e16 (MD5) / Made available in DSpace on 2016-01-05T20:29:30Z (GMT). No. of bitstreams: 1 GiocondaDiasRodriguesLeao_TESE.pdf: 44025376 bytes, checksum: 9da025324f9108141027f1f3a1cc3e16 (MD5) Previous issue date: 2013-05-17 / Background & Aims: HFE-associated Hereditary Hemochromatosis (HH) is one of the most frequent autosomal recessive disease in the caucasian population, caused by the high absorption and deposition of iron in several organs. This accumulation results in several clinical complications such as cirrhosis, arthritis, cardiopathies, diabetes, sexual disorders and skin darkening. Although most of the cases are homozygous individuals for the C282Y mutation, another two mutations, H63D and S65C, have been reported to be associated with milder forms of the disease. The objective is to avaluate the distribution of C282Y, H63D and S65C mutations in the HFE gene in patients with suspected HH in the state of Rio Grande do Norte, Brazil. Methods: Samples of peripheral blood were taken from 335 patients originating from Natal-RN, a city in northeastern Brazil with suspected of HH and which were screened for the HFE gene C282Y, H63D and S65C mutations, using molecular genetics assays (Polymerase Chain Reaction- Restriction Fragments Length Polymorphism). The main criterion for including such patients in the study was the increasing of persistent serum ferritin in individuals aged between 18 and 70 or older, both males and females. As to the exclusion criteria, individuals holding hemolytical anemia, talassemy and previously report of blood transfusion did not take part of the study. Results: Out of the 335 patients studied, 143 patients showed absence of mutation and 195 showed some kind of mutation in the HFE gene: 07/335 (2,08%) were homozigous C282Y, 25/335 heterozygous C282Y, 25/335 (7,46%) were homozigous H63D, 115/335 (34,32%) heterozygous H63D, 5/335 (1,48%) heterozygous S65D, 11/ 335 (3,28%) and were double heterozygous (H63D/C282Y). None patients were Homozygous S65D and S65D heterozygous (S65D/H63D and S65D/C282Y). Conclusions. The distribution of the HFE gene C282Y, H63D and S65C mutations found in our group matches the tendencies observed in other European countries. Due to the high prevalence of hemochromatosis, its seriousness and easy treatment, the genetic diagnosis of HH has become a dream, especially in the high risk group.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-03-12T15:13:48Z No. of bitstreams: 1 JoaoBatistaFigueredoDeOliveira_DISSERT.pdf: 2360795 bytes, checksum: 9eb1bc700876f6b50145bb832a02c344 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-03-15T14:36:30Z (GMT) No. of bitstreams: 1 JoaoBatistaFigueredoDeOliveira_DISSERT.pdf: 2360795 bytes, checksum: 9eb1bc700876f6b50145bb832a02c344 (MD5) / Made available in DSpace on 2018-03-15T14:36:30Z (GMT). No. of bitstreams: 1 JoaoBatistaFigueredoDeOliveira_DISSERT.pdf: 2360795 bytes, checksum: 9eb1bc700876f6b50145bb832a02c344 (MD5) Previous issue date: 2016-01-29 / Esta pesquisa ? uma cartografia, que tem por fundamento as abordagens de Deleuze e Guattari, realizada na Pra?a Mits, na cidade de Natal-RN, composta por juventudes LGBTTI. Nas reflex?es que comp?em o texto, pensamos aquela realidade, como espa?o de pr?ticas n?mades, fazendo uso do conceito de nomadismo formulado por Deleuze e Guattari. Quanto ? constru??o do objeto - ?corpos em muta??es? - ela se deu a partir das leituras de te?ricas/te?ricos e dos efeitos das afeta??es em campo sobre n?s pr?prios. Por essa constru??o, buscamos compreender os corpos como uma amplitude que abriga subjetividade, organismo, multid?o etc., na medida em que ?amos constatando, atrav?s da empiria que, de algum modo, essas dimens?es se atravessam. Utilizamos o termo muta??es, baseados nos relatos e observa??es de campo e focando as transforma??es que as/os jovens se permitem experimentar. A hip?tese norteadora ? que a Pra?a Mits se constitui em um espa?o que possibilita essas muta??es e que favorece a busca por express?es socioculturais das juventudes, que ocupam semanalmente aquele espa?o que, por sua vez, se constitui em um lugar de multiplicidades/diversidades em constantes movimentos de conex?es. / This research is a cartography, which is based in the approaches of Gilles Deleuze and Felix Guattari, developed in the Mits square, situated at Natal-RN city, composed of groups of youngs LGBTTI. Through the reflexions in the text, we think that reality as space of nomad practices, using from the Deleuze and Guattarriconcept of nomadism. About the object's construction ? ?bodies in changing? -it happened by the reading of theorical and of the effects of affectations in camp upon ourselves. By this construction, we try to comprehend the bodies as an amplitude which houses subjectivity, organism, crowd, etc., in that we were finding, by the empirical look that, in someway, these dimensions cross themselves. We use the term "mutation/changing" based on field's reports and observations and focusing the transformations that the young ones allow themselves to experiment. The guiding hypothesis is that the Mits Square constitute a space that allows this changing and encourages the search for social and cultural expressions of youths that weekly engross that space which, in other hand,is constitutedin a place of multiplicities/diversities in constant motion connections.

The giant barrel sponge, Xestospongia muta is a prominent component in southeast Florida reef communities and is often injured from anthropogenic or natural disturbances. The resulting complete or partial shearing of X. muta barrels frequently leads to mortality of loose fragments that do not typically reattach. This study examines the degree to which sponge size and injury severity affects recovery and growth, and explores artificial reattachment success and growth of fragments. In June 2008, 65 sponges were subjected to injury treatments of approximately 50% or 90% barrel removal. A new, simple method was developed to secure these removed barrels (fragments) to the substrate. For 15 months, all donor, fragment and control sponges were monitored for survival, recovery and growth (change in height), and fragments were additionally monitored for reattachment. All injured sponges showed signs of recovery within 1 month. Within 7 months the reattachment method had already proved successful with more than 80% of all fragments becoming attached; final reattachment success after 15 months was 87%. Survival remained high for donors (97%), fragments (94%) and controls (97%). Size class and injury treatment did not significantly affect survival for donors. However, larger fragments had significantly lower survival and reduced reattachment success. Fragment growth rates were significantly lower than donor and control sponges. All sponges showed significant increase in height from post-cutting to the final monitoring. No injured donors reached pre-cut heights, however those from the smaller size class are projected to reach pre-injury heights faster than the larger size class.

The genotoxicity of energetic compounds (i.e., explosives) that are known to be present in contaminated soils at military training sites has not been extensively investigated. Thus, the Salmonella mutagenicity and Muta(TM)Mouse assays were employed as in vitro assays to examine the mutagenic activity of twelve explosive compounds, as well as three soil samples from Canadian Forces Base Petawawa. Salmonella analyses employed strains TA98 (frameshift mutations) and TA100 (base-pair substitution mutations), as well as the metabolically-enhanced YG1041 (TA98 background) and YG1042 (TA100 background), with and without exogenous metabolic activation (S9). For Salmonella analyses, the results indicate that ten of the explosive compounds were mutagenic, and consistently elicited direct-acting, base-pair substitution activity. All three soil samples were also observed to be mutagenic, eliciting direct-acting, frameshift activity. Mutagenic potencies were significantly higher on the metabolically-enhanced strains for all compounds and soil samples. For Muta(TM)Mouse analyses on FE1 cells, the results indicate that the majority of explosive compounds did not exhibit mutagenic activity. All three soil samples elicited significant positive responses (PET 1 and PET 3 without S9, and PET 2 with S9), and although there is some evidence of a concentration-related trend, the responses were weak. Correspondence of the mutagenic activity observed with the two assay systems, for both the explosive compounds and soil samples, was negligible. The differential response is likely due to differences in metabolic capacity between the two assay systems. Furthermore, it is likely that there are unidentified compounds present in these soil samples that are, at least in part, responsible for the observed mutagenic activity. Additional testing of other explosive compounds, as well as soil samples from other military training sites, using a variety of in vitro and in vivo assays, is warranted in order to reliably estimate mutagenic hazard and subsequently assess risk to human health.

Willow grouse ( Lagopus lagopus) and rock ptarmigan (Lagopus muta) are popular game birds in Sweden. I have analysed hunting statistics from Jämtland county in Sweden and investigated if there is a significant correlation between willow grouse density and Catch-Per-Unit-Effort (CPUE) by nationalities and type of permits. The included nationalities were Swedish, Nordic and non-Nordic hunters. Type of permits that were analysed were day-, municipality and county permits. There was no correlation between the grouse density and CPUE variables. However, significant correlations were found when correlating yearly number of harvested grouse  with the CPUE variables. For willow grouse, significant correlations were found between Swedish, Non-Nordic, day and municipality permit hunters and for rock ptarmigan, Swedish, Nordic, day and municipality permit hunters. I also found that international hunters was more effective than Swedish hunters, as well as hunters with day permit, which was true for both species. There were also differences between the CPUE variables between two time periods, 2004-2012 and 2013-2021. In the second period, the CPUE of Swedish hunters, day and municipality permit hunters have decreased for willow grouse. The CPUE also decreased for non-Nordic during the second period for rock ptarmigans. Significant difference in hunting pressure were also found between the two time periods, where the later period had slightly higher hunting pressure than the first period. The results of this study strengthened previous studies where no correlation has been found between the grouse density and CPUE.

No description available.

Submitted by Kelson (kelson@ucb.br) on 2016-08-04T19:02:35Z No. of bitstreams: 1 TulioMachadoVianaDissertacao2015.pdf: 987297 bytes, checksum: bdacb68ff6e4820d3368ef3bf49e7a8a (MD5) / Made available in DSpace on 2016-08-04T19:02:35Z (GMT). No. of bitstreams: 1 TulioMachadoVianaDissertacao2015.pdf: 987297 bytes, checksum: bdacb68ff6e4820d3368ef3bf49e7a8a (MD5) Previous issue date: 2015-12-05 / This work aims to discuss, from the analysis of the Rcl 4374/PE, the use of the constitutional complaint as a instrument processual able to promote the update of the jurisprudence of the Supreme Court, which are firmed under abstract judicial review of the standards. It assumes that the Court???s decisions remain open to a constant process of reinterpretation, by itself, and the constitutional complaint can be the locus for assessment of those changes. It understands that the Constitution of a State, under the guise of being stable, can???t stifle in a way that fails to meet the interests of its people, leading to an institutional breakdown. At the same time, it cannot be subject to a process of deformation through constant changes in its text, destabilizing the legal relations. The question which arises, therefore, is the idea that the Constitution must be interpreted in a temporal context in which required the solution of a given case. Similarly, the judicial decisions which care about the relationship between the standards, whereas continuing legal relationship, aren???t precluded from being modified when there is a material change of context. So, when considering the occurrence of substantial changes in the factual relationships or general legal concept, this reinterpretative process can lead to an evolution in the jurisprudence set in an abstract control of the standards and result in an unconstitutionality, when it was considered constitutional before. Thus, it is proposed the understanding that the legal standards suffer a permanent process of updating of their meaning, which is why the study of the phenomenon of constitutional mutation and interpretation arises as being of great relevance for this update take place. The constitutional mutation is recognized as one of the most important contemporary discussions in Law, by providing a radical change in the interpretation of the Constitution, allowing its renewal, at the light of the social evolution, and offering meanings in line with the current reality / O presente trabalho discute, com base na an??lise da Rcl 4374/PE, o uso da reclama????o constitucional como um instrumento processual apto a promover a atualiza????o da jurisprud??ncia do Supremo Tribunal Federal firmada em sede controle abstrato de constitucionalidade das normas. Parte-se da premissa de que as decis??es da Corte permanecem abertas a um constante processo de reinterpreta????o pelo pr??prio Tribunal, e que a reclama????o constitucional pode ser o locus de aprecia????o dessas mudan??as. A Constitui????o de um Estado, sob o pretexto de ser est??vel, n??o pode se engessar de tal forma que deixe de atender aos interesses do seu povo, levando a uma ruptura institucional. Ao mesmo tempo, n??o pode estar sujeita a um processo de deforma????o por meio de constantes altera????es do seu texto, desestabilizando as rela????es jur??dicas. A quest??o que urge, desse modo, ?? a concep????o de que a Constitui????o deve ser interpretada no contexto temporal em que necess??ria a solu????o de uma determinada controv??rsia. No mesmo sentido, as decis??es judiciais que cuidem de rela????o entre normas, enquanto rela????o jur??dica continuada, n??o est??o impedidas de serem modificadas quando houver altera????o de contexto relevante. Assim, ao considerar a ocorr??ncia de mudan??as substanciais nas rela????es f??ticas ou da concep????o jur??dica geral, esse processo reinterpretativo pode conduzir a uma evolu????o da jurisprud??ncia firmada em controle abstrato de normas e resultar na declara????o de inconstitucionalidade de norma anteriormente declarada constitucional. Com isso, prop??e-se a compreens??o de que as normas jur??dicas sofrem um processo de atualiza????o permanente dos seus significados, raz??o pela qual o estudo do fen??meno da muta????o constitucional e da intepreta????o emerge como sendo de grande relev??ncia para que essa atualiza????o ocorra. Reconhece a muta????o constitucional como uma das discuss??es contempor??neas mais importantes do direito por ensejar uma mudan??a radical na interpreta????o da Constitui????o, permitindo sua renova????o ?? luz da evolu????o social e oferecendo-lhe significados consent??neos com a realidade atual

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-09-06T20:24:13Z No. of bitstreams: 1 ThiagoAndreCidral_DISSERT.pdf: 3578139 bytes, checksum: bcb8c85feab01e028e946691a0776a04 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-09-06T23:31:16Z (GMT) No. of bitstreams: 1 ThiagoAndreCidral_DISSERT.pdf: 3578139 bytes, checksum: bcb8c85feab01e028e946691a0776a04 (MD5) / Made available in DSpace on 2016-09-06T23:31:16Z (GMT). No. of bitstreams: 1 ThiagoAndreCidral_DISSERT.pdf: 3578139 bytes, checksum: bcb8c85feab01e028e946691a0776a04 (MD5) Previous issue date: 2016-01-15 / Os Estafilococos Coagulase Negativos (ECN) s?o microrganismos pertencentes ? microbiota normal da pele e de mucosas dos seres humanos e de animais. A maioria das infec??es causadas por ECN est?o relacionadas ao uso de dispositivos m?dicos invasivos que ao lesionar a integridade da pele servem de base para a forma??o de biofilmes, um importante fator de virul?ncia. Grande parte dos isolados de coagulase negativo s?o provenientes de hemoculturas e pontas de cateter e nos ?ltimos anos vem se tornando um grave problema no que diz respeito ? antibioticoterapia, em virtude do n?mero elevado de cepas multirresistentes descritas. O objetivo deste trabalho foi pesquisar resist?ncia ? linezolida em estafilococos coagulase negativos resistentes ? meticilina isolados de ponta de cateter e hemocultura de hospitais p?blicos e privados da cidade do Natal. Os isolados bacterianos foram coletados a partir de demanda espont?nea em Hospitais P?blicos e Privados. O g?nero Staphylococcus foi confirmado atrav?s dos testes de rotina como colora??o de Gram, prova da catalase da coagulase livre. A identifica??o a n?vel de esp?cie foi realizada atrav?s de testes bioqu?micos convencionais. Algumas amostras tiveram sua identifica??o confirmada pelos sistemas VITEK 2 e MALDI-TOF. O perfil de resist?ncia aos antimicrobianos foi avaliado atrav?s da t?cnica de disco-difus?o (CLSI 2013). A Concentra??o Inibit?ria M?nima para vancomicina e linezolida foi determinada atrav?s do uso de E-test e a presen?a dos genes mecA e cfr foi confirmada pela t?cnica da Rea??o em Cadeia da Polimerase. Algumas amostras tiveram a regi?o V da subunidade 23S do gene do rRNA sequenciadas e analisadas. Posteriormente, as mesmas foram submetidas a t?cnica do PFGE para determina??o do seu pulsotipo. Dos 43 estafilococos coagulase negativos resistentes ? oxacilina inclu?dos neste estudo, 33 (77%) foram identificados como S. epidermidis, 6 (14%) como S. haemolyticus, 3 (7%) como S. homins e 1 (2%) como S. capitis. Os isolados de hemocultura representaram 86% (37) e os de ponta de cateter 14% (6). As amostras apresentaram um perfil de multirresist?ncia, uma vez que 42 dos 43 isolados apresentaram resist?ncia ? 4 ou mais classes de drogas. Todas apresentaram o gene mecA. Nenhuma amostra apresentou resist?ncia ? vancomicina. Tr?s cepas de S. hominis e duas de S. epidermidis, apresentaram resist?ncia ? linezolida com CIM variando entre 6 e 64 ?L/mL. Quando investigadas, apresentaram duas muta??es pontuais (C2190T e G2603T) na regi?o V do gene para rRNA 23S. Nenhuma destas apresentou o gene cfr. O PFGE dos S. hominis revelou a presen?a de um ?nico pulsotipo em 3 hospitais, enquanto n?o foi encontrado semelhan?a gen?tica entre os S. epidermidis. Estes achados destacam a import?ncia da vigil?ncia continuada em rela??o a resist?ncia a linezolida no g?nero Staphylococcus. / Coagulase Negative Staphylococci (CoNS) belong to the normal microbiota of the skin and mucous membranes of humans and animals. The most of the infections caused by CoNS are a serious problem, since an elevated number of multi-drug resistant strains. The objective of this study was to investigate resistance to linezolid in methicillin-resistant coagulase negative staphylococci isolates from hospitals in the city of Natal. Bacterial samples were collected from spontaneous demand from Public and Private Hospitals of the city of Natal-RN. The identification staphylococci of the species were conducted by conventional biochemical. Some Samples had the identification to the species level confirmed by automated methodologies VITEK 2? and VITEK MS?. The resistance profile was evaluated with use of the disk diffusion technique (CLSI, 2013). The MIC to vancomycin and linezolid were determined by using E-test method. The antimicrobial resistance profile was evaluated by disk diffusion technique (CLSI 2013). The Minimum Inhibitory Concentration linezolid and vancomycin were determined by using E-test and the presence of the mecA gene and cfr was confirmed by the technique of Polymerase Chain Reaction. Some samples had the V region of the subunit 23S rRNA gene sequenced and subjected to PFGE technique for determining its pulsotype. Of the 43 coagulase negative staphylococci resistant to methicillin included in this study, 33 (77%) were identified as S. epidermidis, 6 (14%) as S. haemolyticus, 3 (7%) as S. homins and 1 (2%) as S. capitis. The catheter tip isolates accounted for 14% (6) and the blood culture 86% (37). Samples showed an alarming resistance profile, since 98% of the isolates were resistant to four or more class drugs. All were positive for mecA gene. No samples were resistant to vancomycin. Three S. hominis and two S. epidermidis exhibited linezolid resistance with MIC ranging from 6 to 64 ?L/mL. None of the samples had the cfr gene. When investigated, they showed two point mutations each (C2190T and G2603T) in the V region of the 23s rRNA gene. None of them was the cfr gene. The S.hominis of PFGE showed the presence of a single pulsotype in three hospitals, suggesting a clonal spread, while it was not found genetic similarity among S. epidermidis. These findings highlight the importance of continued vigilance of linezolid resistance in the genus Staphylococcus.

Made available in DSpace on 2016-06-02T20:21:30Z (GMT). No. of bitstreams: 1 5001.pdf: 2740278 bytes, checksum: 9d646e9df12b2864a43bac973517db51 (MD5) Previous issue date: 2013-02-25 / Universidade Federal de Sao Carlos / A serpente Lachesis muta rhombeata (Viperidae), popularmente conhecida como surucucu pico-de-jaca, é uma subespécie de Lachesis muta endêmica na Mata Atlântica brasileira, podendo ser encontrada do Ceará até o Rio de Janeiro. O gênero Lachesis apresenta uma peçonha complexa que engloba diferentes proteínas que possuem uma ampla variedade de atividades biológicas, como por exemplo, aquelas que afetam os fatores da coagulação sanguínea, agem na fibrinólise, provocam uma ação inflamatória no local da picada, e induzem um choque hipotensivo. Com o auxílio de técnicas proteômicas e de Bioinformática, este projeto teve como objetivo principal analisar o proteoma da peçonha de L. m. rhombeata, identificando as principais famílias proteicas presentes. Para isso, 200μg da peçonha desta serpente foram aplicados em géis bidimensionais-2D. As proteínas obtidas foram extraídas dos géis, tratadas, e posteriormente identificadas e analisadas por meio de espectrometria de massas (MALDI-TOF/TOF). Os arquivos brutos provenientes dessas análises foram aplicados no programa MASCOT utilizando para a identificação proteica o banco de dados SwissProt e como especificação taxonômica o banco de dados SNAKES contido no site do NCBI. Foram identificadas 11 famílias proteicas: PLA2s (37,93%), inibidores de proteases (22,41%), proteínas de sinalização intracelular (13,79%), NGFs (5,17%), metaloproteases (3,45%), fatores associados à DDB1 e CUL4 (3,45%), oxidorredutases (3,45%), proteínas de transdução de sinal (1,72%), componentes do complemento (1,72%), proteínas NipSnap (1,72%) e proteínas lin-7 (1,72%), das quais a grande maioria é de função metabólica e ajuda na difusão/entrada dos componentes tóxicos no organismo da vítima/presa. Cerca de 1-5mg da mesma peçonha também foram analisados por meio de FPLC e sequenciamento proteico e as proteínas obtidas foram identificadas por meio de busca no banco de dados nr (nonredundant protein sequences) do NCBI utilizando a ferramenta blast-p. As seguintes famílias proteicas foram encontradas: serinoproteases (30,03%), metaloproteases (24,17%), BPPs (19,64%), PLA2s (14,95%), lectinas tipo C (1,94%), LAAOs (1,40%) e metaloendopeptidases (0,73%), ressaltando a toxicidade da peçonha. Os dois resultados obtidos foram comparados com proteomas de outras serpentes do gênero Lachesis encontrados na literatura. Observou-se que há uma grande similaridade entre os proteomas das peçonhas deste gênero, mas que alguns componentes proteicos são diferenciais e assim, podem ajudar na taxonomia destas serpentes, mantendo-as em suas respectivas espécies e subespécies. Além disso, a peçonha também foi analisada por eletroforese unidimensional (SDS-PAGE) para avaliar se o espécime em estudo era um neonato, um juvenil ou um adulto. A presença de uma proteína similar à mutalisina II (metaloprotease) confirmou que a L. m. rhombeata utilizada neste estudo era uma adulta. O conhecimento do proteoma da peçonha de L. m. rhombeata auxiliará (1) os estudos envolvendo a Sistemática do gênero e (2) a produção de novos fármacos que poderão ser desenvolvidos para o tratamento de diversas doenças.

Orientador: Selma Maria de Almeida-Santos / Resumo: O conjunto de características manifestadas por uma espécie que levam ao sucesso reprodutivo pode ser chamado de estratégias reprodutivas. Isto inclui, por exemplo, o período reprodutivo, o modo reprodutivo, a sincronia reprodutiva entre machos e fêmeas, o tempo de fertilização e a frequência reprodutiva. O gênero Lachesis é composto de quatro espécies ovíparas restritas às florestas úmidas da América Central e América do Sul. Popularmente conhecidas como surucucu, são um grupo de importância médica, tal como Bothrops e Crotalus. Lachesis muta é a espécie com a maior distribuição, e ocorre na América no Sul, na Mata Atlântica e na Floresta Amazônica. Ao contrário de Bothrops e Crotalus, estudos sobre biologia reprodutiva para o gênero Lachesis são, em sua maioria, desenvolvidos em cativeiro e com foco no comportamento reprodutivo. Propomos, então, descrever em dois capítulos aspectos da biologia reprodutiva da espécie Lachesis muta investigados a partir da anatomia e morfologia de seus órgãos reprodutivos ao longo das estações do ano. No Capítulo 1, investigamos a biologia reprodutiva de L. muta da Floresta Amazônica e da Mata Atlântica brasileira a partir de dados de biometria e histologia de animais preservados em coleções biológicas e provenientes da natureza. No Capítulo 2, descrevemos o oviduto feminino em diferentes estágios do ciclo reprodutivo. Comparando tanto as duas populações quanto a espécie com outros crotalíneos neotropicais, nosso objetivo foi investigar possív... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The set of characteristics manifested by a species leading to reproductive success can be called reproductive strategies. These include, for example, the reproductive period, the reproductive mode, the reproductive synchrony between males and females, the time of fertilization, and the reproductive frequency. The genus Lachesis comprises four oviparous species restricted to Central and South American rainforests and popularly known as bushmasters, and is a group of medical importance, as are Bothrops and Crotalus. Lachesis muta is the most widely distributed and occurs in South America, at the Atlantic Forest and the Amazon Rainforest.Unlike Bothrops and Crotalus, reproductive biology studies for the genus Lachesis weremostly developed in captivity, focusing mainly on reproductive behavior. We propose to describe in two chapters aspects of the reproductive biology of the Lachesis muta species investigated from the anatomy and morphology of its reproductive organs over the seasons of the year. In Chapter 1, we investigate the reproductive biology of L. muta by biometric and histology data from biological collections, focusing on individuals collected in the wild. In Chapter 2, we describe the female oviduct at different stages of the reproductive cycle. Comparing both populations and species with the other neotropical crotalines, we aim to investigate possibles patterns for the Viperidae group, as well as the hypothesis of the influence of abiotic factors on the snake reproduc... (Complete abstract click electronic access below) / Mestre