This thesis describes the work of three research projects, the background research that motivated the work, and the resultant project findings. The three projects concerned: (i) Geometric stochastic resonance in a double cavity, (ii) Synchronisation of geometric stochastic resonance by a bi-harmonic drive, and (iii) Rectification of Brownian particles with oscillating radii in asymmetric corrugated channels. In the project 'Geometric stochastic resonance in a double cavity', we investigated synchronisation processes for the geometric stochastic resonance of particles diffusing across a porous membrane and subject to a periodic driving force. Non-interacting particle currents were driven through a symmetric membrane pore either parallel or perpendicular to the membrane. Then, harmonic mixing spectral current components were generated by the combined action of parallel and perpendicular drives. The role of the repulsive interaction of particles as a controlling factor with potential applications to the transport of colloids and biological molecules through narrow pores was also investigated. In 'Synchronisation of geometric stochastic resonance by a bi-harmonic drive', we simulated the stochastic dynamics of an elliptical particle using the Langevin equation. The particle was simultaneously driven by low and high frequency harmonic drives across a porous inter-cavity membrane. It was observed that the particle oscillated out of phase with the low frequency drive. This effect was due to the absolute negative mobility the particle would have exhibited if the low frequency drive had been replaced by a dc static force. It was also observed that the magnitude of this out-of-phase stochastic resonance depends on how the combined action of the driving forces and noise fluctuations affect the particle orientation, and as such was shown to be sensitive to the particle shape. This emphasises the importance of particle geometry, in addition to chamber geometry, to the realisation and optimisation of geometric stochastic resonance. In the project 'Rectification of Brownian particles with oscillating radii in asymmetric corrugated channels', we simulated the transport of a Brownian particle with an oscillating radius freely diffusing in an asymmetric corrugated channel over a range of driving forces for a series of temperatures and angular frequencies of radial oscillation. It was observed that there was a strong influence of self-oscillation frequency upon the average particle velocity. This effect can be used to control rectification of biologically active particles as well as for their separation according to their activity, for instance in the separation of living and dead cells. The background research is described in Chapter One and the research findings are described along with their related projects in Chapters Two and Three.

Ever since the big recession of the world economy 2007, the central banks in Europe have struggled to regain financial stability. Their goals have been hard to reach and 2014 The European Central bank (ECB) introduced negative interest rates for the first time in the world history. However, today, year 2016, many countries still have not been able to reach their inflation target. During this time with expansive monetary policies, many European Union (EU) members have faced rising level of household debts to GDP. This study focus on EU-members and uses a Vector Autoregressive method, Granger causality test and an impulse-response test to give a greater understanding about the association between the level of household debt and interest rate. Further, it aims to investigate if the negative interest rate has an impact on that association. However, our empirical results show that there is a significant negative association between the level of household debt and the interest rate in Austria, Belgium, Bulgaria, Finland, Germany, Italy, Poland, Romania, Slovakia, Spain and Sweden. Further, they show that there is a granger causality from the interest rate to the level of household debt for Belgium, Finland, Germany, Poland, Slovakia and Sweden. For all these countries, our findings show that a shock in the interest rate have a short-term effect on the level of household debt. Lastly, we found no statistical significant evidence for that the negative association between the interest rate and the level of household debt does increase when the interest rate is negative.

Doctor of Philosophy / Department of Agricultural Economics / John A. (Sean) Fox / The objective of this thesis is to investigate three design issues in experimental auctions: 1) the effects of allowing negative bids for a privately valued good, 2) the effects of introducing additional alternatives (substitutes) for the auctioned good in an endowment auction, and 3) respondent behavior in acquiring information. The thesis consists of three papers examining those issues. The first paper examines participants’ bidding behavior when negative bids are allowed for privately valued goods in an experimental auction. We focus on two questions: i) whether subjects with negative values tend to bid strategically – either overbidding or underbidding in an effort to enhancing earnings, and ii) the performance of random nth and 5th price auctions. We find that: a) WTP bids are demand revealing, b) subjects tend to underbid WTA values, c) controlling for risk attitude partially explains the bias in WTA bids, and d) negative values from random nth auctions tend to be below those from 5th price auctions. In the second paper we 1) investigate the effect of the availability of varying numbers of alternatives (substitutes) for a privately valued good on participants’ bidding behavior, and 2) identify whether the availability of additional alternatives: a) impacts the value of product information, and b) impacts the effect of new information on product valuations. We find that: a) allowing additional alternatives in a private value auction does not significantly decrease subjects’ bids, and b) the presence of additional alternatives in the auction decreases both the value and effect of product information. The third paper examines the effect of acquired information on auction participants’ bidding behavior. We focus on three questions: i) how subjects choose/value different types of information, ii) whether the value of acquired information about a product influences the subsequent valuation of the product itself, and iii) whether the effects of acquired information differ from those of exogenously provided information. We find that: a) subjects’ behaviors of acquiring different types of information about the product are influenced by their heterogeneous characteristics (i.e. prior beliefs, risk attitudes, prior knowledge, etc.), b) subjects place more weight on acquired information than on provided information in their decision-making process, and c) individual subjects have different values of information which caused different impacts on product valuation.

Clarifying processes associated with emerging externalizing behavior problems during early childhood was the focus of this study. Data were collected from 100 parent-child dyads when children were 2, 3, and 4 years. An incremental risk model was hypothesized to explain the emergence of externalizing behavior problems. Theoretically, children's temperamental propensity towards negative emotional reactivity increases risk for noncompliance, noncompliance that increases risk for externalizing behaviors by age 4. Parenting was identified as the mechanism by which children's progression along the incremental risk pathway is amplified or minimized; progression is only expected under conditions of harsh parenting. No statistical support emerged for the incremental risk model or the moderational effects of harsh parenting. Harsh parenting was a statistically significant predictor of children's noncompliance one year later. Implications of the current findings for future research are discussed.

Losing a loved one – a child or a spouse –is described as one of the most stressful or negative experience of a person’s life. Aging is associated with a higher risk of the death of close family members, yet few studies have investigated the impact of such losses on different health outcomes either by type of loss or by the combined loss of both a child and a spouse. This thesis is based on three studies examining the effect of bereavement on the health of older adults who have lost a child, spouse, or both and whether the different losses were associated with Life Satisfaction (LS) or mortality. The sample was collected from the Swedish National Study of Aging and Care (SNAC). The results showed that the loss of a child, spouse or both was experienced as among the three most important negative life events in the bereaved groups. About 70% of those bereaved of a child or a spouse mentioned these losses as among their three most important negative life experiences. In the child-and-spouse-bereaved group, 48% mentioned both losses while 40% mentioned only the loss of a child or a spouse, but not both. However, only marginally effects on LS and mortality after child, spouse or child-spouse bereavement in older adults was found. Longer time since the loss was associated with higher LS and lower mortality risk, and type of loss did not seem to determine LS or mortality. Gender differences were found: child-, spouse and child-and-spouse-bereaved men had lower LS than the corresponding groups of bereaved women, and furthermore, child-bereaved men had an increased mortality risk compared to child-bereaved women. Finally, significantly more women in the child-and-spouse-bereaved group compared to the men in this group, mentioned the loss of their child but not the spouse, among the three most important negative life events.

A unique undosciribed gramnegative rod is extensively characterized in this study. The cells of this unusual water isolate measure 1.2 X 6.5 microns, The most distinguishing characteristic of the bacterium is a polar tuft of 35-40 flagella that aggregate to function as a single organelle which is visible under phase contrast. Aging cells deposit poly- -hydroxybutyric acid granules which are bound by an inclusion membrane made up of four distinct layers. It also possesses an unusual exterior membrane outside the cell wall which contains large fibrils of protein running at a slight angle to the longitudinal axis of the cell. The guanosine-cytosine ratio was found to be 62.2$. The organism's taxonomic position was further investigated by immunological, morphological, and biochemical methods. It was found to be most closely akin to members of the genus Pseudo onas, although somewhat divergent from other species classified in this genus. After careful evaluation of the findings obtained during this study, the new bacterium was subsequently named Pseudomonas multiflagella.

O vírus de Leishmania 1-4 ( do inglês Leishmania RNA virus 1-4 ou LRV1-4) é um vírus da família Totiviridae, e que possui capsídeo icosaédrico e RNA dupla-fita que codifica duas proteínas (proteína capsidial e RNA polimerase). Dados recentes indicam o envolvimento do LRV1-4 na patogênese de Leishmania no hospedeiro humano, tornando seu estudo de fundamental importância para o entendimento dessa doença e de seu papel na relação parasito-hospedeiro. Há relatos sobre a purificação do vírus a partir do seu hospedeiro natural (Leishmania guyanensis) e a partir de sistemas de expressão heteróloga. Este trabalho tem por objetivo estabelecer os métodos de purificação para posteriores estudos estruturais por Microscopia Eletrônica de Transmissão por Contraste Negativo (NS-TEM) e por Crio-Microscopia Eletrônica (Cryo-EM). Os estudos aqui propostos irão permitir a construção de um modelo estrutural do capsídeo do LRV1-4 e sua identificação correta dentre os totivírus. Além das contribuições ao conhecimento da biologia/patogenia do LRV1-4 este estudo representa a primeira caracterização estrutural de um capsídeo viral realizada no Brasil, e assim um avanço importante para a área de virologia e biologia estrutural no pais. Foram realizadas ultracentrifugações biológicas, utilizando gradientes de sacarose, para a purificação do vírus a partir do extrato celular de L. guyanensis. As frações que apresentaram RNA viral foram analisadas por Microscopia Eletrônica de Transmissão (Campinas LNNano CNPEM). Além disso, foram realizadas tentativas de expressar a proteína do capsídeo (ORF2) em Leishmania tarentolae e Escherichia coli. Foram também realizados esforços para a obtenção de anticorpos a partir de peptídeos sintetizados após análise computacional da sequência de aminoácidos da proteína do capsídeo. As amostras obtidas a partir do hospedeiro natural do vírus se apresentaram heterogêneas quando analisadas por NS-TEM, de modo que não foi possível a realização de uma análise estrutural. Porém, a presença de partículas do tamanho esperado para o vírus em amostras em que foi detectado o RNA viral indicam que são necessários esforços para obtenção de uma amostra de maior pureza e homogênea. Além disso, não foi possível obter a proteína do capsídeo nos sistemas de expressão heteróloga. A presença de 25 resíduos de cisteína pode estar levando a proteína à degradação rápida em bactéria. Os experimentos de expressão em células de Leishmania ainda não foram conclusivos. Foi obtido um anticorpo anti-peptídeo que reconhece a proteína do capsídeo, tornando possíveis experimentos como imunolocalização e imunoprecipitação do vírus. / The Leishmania RNA virus 1-4 (LRV1-4) belongs to the Totiviridae family. It has an icosahedral capsid and a double-strand RNA encoding two proteins (capsid protein and RNA polymerase). Recent data indicate the involvement of LRV1-4 in the pathogenesis of Leishmania in the human host, making their study of fundamental importance for the understanding of this disease and its role in host-parasite relationship. There are reports on the purification of the virus from its natural host (Leishmania guyanensis) and from the same heterologous expression systems such as Escherichia coli.This work aims to stablish purification methods for further structural studies by Negative Stain Transmission Microscopy (NS-TEM) and Cryo-Electron Microscopy (Cryo-EM). The studies proposed here will allow the construction of a structural model of the coat protein of LRV1-4 and their correct identification amongst the Totiviridae. In addition to the contributions to the knowledge of the biology and pathogenesis of LRV1-4, this study represents the first structural characterization of a viral capsid held in Brazil and thus an important step forward for the field of virology and structural biology in the country. Sucrose for virus purification gradients were performed from the cell extract of L. guyanensis. Fractions that showed viral RNA were analised by Transmission Electron Microscopy (Campinas LNNano - CNPEM). Furthermore, attempts have been made to express the capsid protein (ORF2) in Leishmania tarentolae and Escherichia coli. There has also been made efforts to obtain antibodies from peptides synthesized accordingly to the computer analysis of the amino acid sequence of the capsid protein. The samples obtained from the natural host of the virus showed a heterogeneous distribution of particles when examined by NS-TEM so that it was not possible to perform a structural analysis. However, the presence of particles of the size expected for the virus particles in samples where the viral RNA was detected indicate that efforts are necessary to obtain a more homogeneous and pure sample. Moreover, it was not possible to obtain the capsid protein in heterologous expression systems. The presence of 25 cysteine residues could have led to the protein rapid degradation in the bacteria host. The expression experiments in Leishmania cells were not yet conclusive. It was also possible to obtain an anti-peptide antibody recognizing the capsid protein, enabling immunoprecipitation and immunolocalization experiments.

Thesis advisor: Donnah Canavan / This study examines the relationship between shyness/social phobia and other personality traits, as manifested in college students. Very few studies have compared shyness and social phobia, and even fewer have contrasted them. However, we believe that there are several important differences between the two. Mainly, one hypothesis is that social phobics long to be more extroverted and, thus, are more conflicted in many aspects of their personalities. Moreover, very few studies have found a conclusive link between shyness or social phobia and self-esteem. One of our main hypotheses is that there is a link between shyness/social phobia and low social self-esteem, although not necessarily overall self-esteem. We sought support for these hypotheses and others in the analysis of data obtained through a survey, composed of several standard questionnaires, as well as original material covering areas of self-image, behaviors, emotions, and personality traits. The results did indicate substantial differences between shyness and social phobia, with social phobics having higher levels of extraversion and, overall, more conflict within themselves. / Thesis (BA) — Boston College, 2006. / Submitted to: Boston College. College of Arts and Sciences. / Discipline: Psychology. / Discipline: College Honors Program.

Thesis advisor: Krzysztof Kempa / Though nature provides a plethora of materials to work with, their properties are very much restricted, forcing severe limitations on the devices that are built from them. A huge portion of current technology stands to be significantly advanced and even revolutionized by the emergence of a new class of “configurable” materials. This class, generally referred to as metamaterials, has become more feasible than ever due to advancements in nanotechnology and fabrication techniques. Notable among nature’s limitations is an ever-positive index of refraction. This barrier has only recently been broken, and the known paths to negative refraction are few and limited. This paper introduces two distinct three-dimensional crystals capable of all-angle negative refraction. One uses the familiar photonic band, while the other is the first of its kind to rely on polaritonic waves. Their mode structures are examined and a set of parameters are chosen at which a negative effective index of refraction can be harnessed for unrestricted sub-wavelength lensing, demonstrated via numerical simulation. This work is expected to enable experimental observation of polaritonic negative refraction and sub-wavelength lensing at microwave frequencies. / Thesis (BS) — Boston College, 2009. / Submitted to: Boston College. College of Arts and Sciences. / Discipline: College Honors Program. / Discipline: Physics.

Foi estudado o efeito de priming negativo associado à supressão do distrator palavra-cor de Stroop (Estudo 1), à supressão do local (Estudo 2) e à identidade do distrator (Estudos 3 e 4). No Estudo 1 constatou-se que a prática prévia em palavras-cor eliminou o efeito da ordem das condições experimentais sobre o índice de priming negativo. No Estudo 2, o efeito de priming negativo foi observado somente no hemicampo direito. A execução concorrente de uma tarefa verbal eliminou os efeitos de lateralidade, mas o efeito de priming negativo permaneceu significante. Estes achados são discutidos em termos de processamento interhemisférico sob condições que exigiriam maior controle da atenção. Os estudos 3 e 4 apresentam tarefas de comparação de pares de dígitos. A versão de papel e lápis da tarefa de comparação de dígitos permitiu avaliar de maneira simples e rápida o efeito de priming negativo. A versão computadorizada, revelou uma interação entre os componentes espacial e de identidade. Ambos os grupos de adultos jovens e idosos revelaram priming negativo nas tarefas de Stroop e de localização espacial. Nas tarefas de identificação do alvo somente os adultos jovens mostraram efeito de priming negativo. Os presentes achados são consistentes com a proposta de mecanismos inibitórios diferenciados na supressão da identidade e de localização espacial. / The development of sensitive and simple tests for the assessment of the negative priming effect has theoretical relevance to the elucidation of selective attention models, and also practical and potential clinical implications. The negative priming effect has been regarded as an index of inhibitory attentional processing and was proposed for the detection of syndromes that involve cognitive impairment. Diminished negative priming was reported in studies of individual differences, developmental stage, and clinical populations. However, evidences suggest that tasks requiring responses to the color feature, location or object identity of the stimuli may comprise distinct types of negative priming tasks. The following studies presents data for computerized and paper-and-pencil tasks to examine negative priming for Stroop color-word, location and identity distractors. All four studies take into account aging effects across the tasks. For comparisons between age-groups, proportional performance scores (ratio) were used. A first study employed a reading-sheet Stroop-color-word task, in which the participant is asked to name the colors of the ink in which words with incongruent color names have been printed. Color-word interference is indicated by increased time to complete the conflicting color-word condition compared with a nonconflicting condition with patches of color or strings of Xs. The greater strength of the interference, when the target ink-color of the present stimulus is the distracting color name of the previous stimulus, is attributed to the negative priming effect. A pilot experiment showed that the order of the list conditions containing unrelated and related stimuli affected the negative priming index. The analysis of data demonstrated that a practice trial in color naming of conflicting color-words before the color-word conditions eliminated the effect of the order of the lists. In addition, there was a reliable Stroop reverse interference after practice in color naming, as indicated by the fact that the incongruent color-ink affected post-test word-reading, whereas it had no effect in the pretest word-reading. With practice procedure, older and younger subjects did not differ in their proportional interference scores, whereas the negative priming and reverse effects were increased for older adults. Study 2 examined the negative-priming effect in a spatial localization task under single- and dual-task conditions. The task required the subject to detect the location of a target letter, ‘O’, while ignoring a distractor letter, ‘X’, when it was present. Significant negative-priming effects were observed under both task conditions, with increased response times for trials in which target location had matched the location of the distractor on the preceding. The magnitude of the negative priming effect was not different for older and younger adults. The performance in the single-task condition showed laterality effects with a right visual field advantage for control and target-alone trials, but not for related trials. In consequence, in the single-task condition, negative priming was observed only for targets displayed in the right hemifield. However, a concurrent digit span task, with a load level that had shown no affect on the dual-task coordination capacity, eliminated the laterality effects, but the negative priming effect remained. These results are considered as neuropsychological evidence that interhemispheric processes may operate under more controlled conditions. Studies 3 and 4 examined negative priming by using an identity-based task that required participants to select the greater of two-digits display or the digit that was paired with an asterisk. Study 3 presents data for a computerized version of the task. Negative-priming was expressed as a slowing in the time to name the digit that had been ignored in the preceding trial, compared to control trials with consecutive targets and distractors always different. Analysis of data revealed that negative priming was reliable only for younger adults, and only when target probe and distractor prime appeared at the same location, suggesting that suppression for location of distractor was underpinning the negative priming effect. However, response latencies for the control trials were facilitated when the target probe and the distractor prime shared the same location. Thus, local suppression affected negative priming for attended distractors with a cost in the response latency for ignored-repetition trials and with a gain in response latency for control trials when the locus of target-probe and distractor-prime was the same. In contrast, older adults’ performance showed local suppression for both ignored-repetition and control trials. This may explain the lack of negative priming for older adults in the digit-comparison task. Study 4 presents data for a new paper-and-pencil version of the digit-comparison task to obtain a practical measure of negative priming that do not require cumbersome technical equipment. In that task, subjects were asked to circle digits that were paired with asterisks and the greater of two digits in a series of digit pairs listed on a sheet of paper. For younger participants, but not for older participants, the time to complete the sheet with related pairs was slower than for unrelated pairs. In addition, the reduced scores of negative priming in older adults were associated with the lowest sustained attention scores from Toulouse-Piéron test. These results suggest that older adults’ performance in the digit-comparison task were mainly related to flexibility and sustained attentional scores, and the lower sustained attentional coefficient seemed to be the best predictor of diminished or reversed negative priming in older adults. Younger adults showed reliable negative priming across all tasks. In contrast, older adults showed negative priming in Stroop and spatial tasks, when compared with younger subjects performance, but reduced negative priming in identity suppression tasks. The findings are consistent with neurophysiological and behavioural evidence that identity and location suppressing may rely on separate inhibitory mechanisms, and that not all of these processes are weakened by factors associated with age.