Through the consideration of Adorno’s theory of ‘negative dialectics’ the existing society of art is in a state of alienation. This idea considers units of similarity abstracted from one another and thereby establishing an opposition; a negative dialectic relationship between creatives. “Instead of belonging to the world, man put himself rationally and critically opposite it...“ (Jencks & Baird, 1969, p. 216). The concept that a future can exist free from alienation lends toward a future of collaborative community considered within the context of cultural production. Culture is not merely the manifestation of the human mind into a creative material medium; it is also a mechanism of interaction developed through social behaviour such as a custom or an idea. Kristeva’s construct, ‘the abject’ (that which is neither subject nor object), is used to define the scope of this project. Producing culture using space is not defining object in space nor is it defining subject in space. The triadic interaction between object, subject and abject is important in understanding the cultural system within the built environment. This is conceptually intended to manifest the communal identity of creatives within the host building. Considering the explicit (whereby knowledge and social interaction are produced) and implicit (which considers action between object and subject) relationships reinforces the argument. The understanding of the relationship between cultural media and mediators is to be explored spatially in the adaptive reuse of the Sunnyside Post Office into a production house and exhibition space, utilising social applications to defining a model for a creative cohesion. / Dissertation (MInt(Prof))--University of Pretoria, 2014. / Architecture / MInt(Prof) / Unrestricted

A mixed-methods approach was utilised in order to explore the impact of group Music Therapy on the negative symptoms of forensic clients with schizophrenia. Eight participants were involved in this exploratory study at Valkenberg hospital in the Western Cape, South Africa. The Music Therapy intervention consisted of eight sessions over a period of six weeks. Qualitative data were collected through video footage, with three excerpts being selected for thick descriptions to be written and, subsequent to the process of therapy, eight semi-structured interviews were conducted with the participants. Quantitative data were generated by structured interviews with the eight participants individually using the PANNS test both before and after the music therapy process. Qualitative data were analysed using thematic analysis, while the quantitative data were presented through descriptive statistical analysis, and a paired sample t-test was conducted. Findings suggest that group Music Therapy affords forensic adult clients in a psychiatric hospital who are diagnosed with schizophrenia benefits in relation to their negative symptoms. The themes that emerged through thematic analysis were ‘Holding’, ‘Positive Experiences of Music Therapy’, ‘Togetherness’, ‘Activation’, ’Flexibility’ and ‘Inflexibility’. The quantitative data shows an overall improvement in the negative symptoms. A paired sample t-test calculates this improvement as too small to be statistically significant. However, the overall improvement shown in the quantitative data suggests the possibility that the group Music Therapy intervention may have effected a gradual positive shift over time and this requires further research. Although participants still displayed negative symptoms in their daily life in the ward, music therapy offered an environment in which well-being could be experienced and expressed. / Mini Dissertation (MMus)--University of Pretoria, 2016. / Music / MMus / Unrestricted

Purpose: This research sought to explore and systematically review the landscape of literature on the topics of workplace gossip and foreign nationals, in order to cre-ate an aerial shot of the areas of investigation favoured by researchers, the methods employed for inquiry, theories applied to the subject matter and finally openings in research for expansion and in-depth examination. Method: A total of 50 initial articles were drawn from elected databases. The data-bases included, Emeralds insight, Google scholar, Oxford, Taylor Francis, PubMed, Research gate, Sage, the South African journal of industrial psychology Springer link and Wiley online. Keyword searches were iteratively formulated, tracked and repli-cated for searches of each database. The yielded articles were originally pitted against predefined inclusion and exclusion criteria; this was done through a prelimi-nary review of the articles’ abstracts. The articles were subsequently grouped based on their research aims. The findings were written out in prose and presented a snap-shot of workplace gossip and foreign national research in the last decade. Findings: A number of themes were raised through the review process. Prosocial gossip works as an informal discipline system, though the processes of isolation and ostracism. Gossip is a viable tool for social control. Organisational identity and low power interact and also result in lower tendencies to gossip as a means of indirect aggression while high power and low organisational identity result in contrary effects. Greater employee identification with the organisation results in superior perceived cohesion. Job related gossip has an influence on employee cynicism and has a me-diating effect on the relationship between abusive supervisors and cynicism. Psycho-logical contract violation is a predictor of job-related gossip and employee cynicism while the presence of an abusive supervisor predicts both job-related and non-job related gossip. Furthermore, non-whites in white-dominated organisations, racial mi-norities and women experience gossip perpetrated by dominate groups. Dominant groups use gossip to discredit, undermine, exact group exclusion, communicate slurs, communicate subjective evaluations as truth, promote negative talk and opin-ions that minorities do not belong at the top of organisations. Minorities respond in two manners, by acquiescing or avoiding confrontation. Regarding immigrant eco-nomic assimilation, immigrants earn 34% less than their native counterparts. Immi-grants experience greater levels of unemployment than the country natives. Interestingly, immigrants seem to sort into low paying organisations. Moreover, longer re-ported occupation in a host country determines annual increases in pay of around 0.9%. Even more so, immigrants who work at majority native organisations report higher earnings. Residential location plays a role in earnings as the discrimination effect, means employers have biases about who they employ based on their residen-tial address. The relationship between immigrant-native isolation and exposure to immigrant neighbours is negative. Immigrants face barriers to integration through the non-recognition of their qualifications and perception of lower productivity. Employers favour local qualifications and accreditations over those held by immigrants. On the contrary, in some instances local qualifications seem to be considered sporadically and immigrants are put up against lesser qualified natives who are part of the organ-isations racial majorities when seeking career advancement. Immigrants assimilate through focusing on the following behaviours, work-related professional culture, un-derstanding workplace. Non-work related: higher exposure to natives in residential neighbourhoods and intermarrying with locals. Literature displayed that the words immigrant and expatriate are synonyms; however, in the existing international man-agement literature they describe two starkly different groups. Expatriates focused on in management literature are male, of Anglo-Saxon decent, hail from developed countries in North America or Western Europe. On the other hand, immigrants are male and female, hail from developing countries, are racial minorities, earn low wag-es and work in feminized roles that are unskilled and semiskilled. Regarding em-ployee wellness, findings showed that job satisfaction is an indicator of wellbeing. Furthermore, job satisfaction has been connected to organisational commitment in employees and has a negative relationship with employee absenteeism and employ-ee inclination to turnover. In general job satisfaction promotes positive behaviours and limits the negative. Environmental factors, job characteristics and work specific personal factors are just three referenced work-related factors linked to immigrant job satisfaction. Recommendations for future research: A definite need is an empirical enquiry into whether foreign nationals are targets of gossip. Though in light of the current study, this seems to be eminent that there is a need for evidence driven commentary re-search. Another suggestion is inquiry into how gossip influences the wellbeing of foreign nationals. Finally, I recommend exploring the question on what impact; gossip has on foreign national assimilation / Dissertation (MCom)--University of Pretoria, 2019. / Human Resource Management / MCom / Unrestricted

archives@tulane.edu / Triple negative breast cancers (TNBCs) are clinically and biologically aggressive, with higher recurrence and metastasis rates compared to other subtypes. Acquisition of a mesenchymal and migratory cell phenotype is consequential process that promotes metastasis. There are no clinically approved small molecule targeted therapies for TNBC; kinases are effective drug targets in cancer research. Although some kinases are known to regulate the mesenchymal phenotype, a large subset of the human kinome is understudied. There are many approaches to discovering novel kinase targets in cancer. Here, a phenotypic screen approach is described to identify understudied kinases using the Published Kinase Inhibitor Set (PKIS). Initial screens using TNBC cell lines (MDA-MB-231, BT549 and MDA-MB-157) identified 36 hits representative of twelve kinase inhibitor chemotypes based on reversal of the mesenchymal cell morphology. Our hits were further prioritized based on gene expression changes of the epithelial marker E-cadherin and migratory behavior. Active compounds were confirmed to reverse EMT on transcript and protein levels with qRT-PCR and Western blot. When pharmacologically similar compounds were more closely examined, different effects on cancer biology were observed (‘active’ versus ‘inactive’ compounds). Based on these observations, a kinase array was employed to compare both the active and inactive compounds to demonstrate how to identify candidate kinases responsible for the EMT reversal. Using this screening approach, small molecule inhibitors from the PKIS library (GSK346294A, GSK448459A, GSK237700A) were identified that were pharmacologically similar that reversed the mesenchymal phenotype in TNBC. These compounds have different biological effects in TNBC, despite having similar pharmacophores. Differential effects of the PKIS compounds on transwell migration, gene (qRT-PCR) and protein (Western blot) expressions, and mammosphere formation in TNBC cells was observed. In follow-up in vivo studies, our most active compound (GSK346294A) suppressed tumorigenesis and metastasis. RNA-sequencing confirmed downregulated pathways induced by GSK346294A treatment in TNBC cells included EMT, cytoskeletal rearrangement and cell cycle regulation. Because these compounds have different off-target activities, this approach can be used to identify candidate unique kinases responsible for the observed effects. NEK5 was one of these kinases candidates. NEK5 function remains understudied in cancer, and even more understudied in breast cancer. This study is the first, to our knowledge, to describe the function of NEK5 in breast cancer, specifically its roles in acquisition of a mesenchymal and migratory cell phenotype. Overexpression of NEK5 promotes a migratory and mesenchymal phenotype, and knockdown with a shRNA construct suppresses this migratory behavior. Data obtained using both qRT-PCR of the knockdown and overexpression cell lines, and follow-up RNA sequencing, revealed NEK5 regulates the PLAU/PAI-1/SRC axis. Furthermore, a role for NEK5 in resistance to SRC-targeting anticancer agents is demonstrated. The work described here demonstrates the utility of a novel approach to identify understudied kinases in cancer, and characterization of these kinases has potential impact in other metastatic diseases not limited to breast cancer. / 1 / Margarite Matossian

Constitutional Court: Negative Legislator? Abstract This diploma thesis deals with the question whether the Constitutional Court of the Czech Republic can be considered as a mere negative legislator in connection with deciding on proposals for annulment of laws or their individual parts according to Art. 87 par. a) of the Constitution or whether the Constitutional Court departs from this position and how. The diploma thesis is divided into theoretical and practical part, while the author seeks to rely mainly on the actual decision-making practice of the Constitutional Court. In the theoretical part, the thesis deals with the concept of the negative legislator and its origin in the ideas of Hans Kelsen. Kelsen's concept of constitutional justice as a negative legislator is presented. In the following subchapters, the theoretical part is devoted to acquainting the reader with the decision-making of the Constitutional Court on petitions to annul laws or their individual parts. The thesis also describes the binding of judges in decision- making and their binding for other subjects. In the practical part, the thesis is divided into six individual chapters, each of which deals separately with one type of decisions that the Constitutional Court deviates from its position as a negative legislator. In each chapter...

This thesis deals with the subject of proving negatives, i. e. proving non-existence of an object, a status or a process. The author of the thesis provides the reader with the definition of a negative and with a list of negatives mentioned in the case law of Czech public authorities. Also, the author analyses selected case law of Czech courts and describes proving negatives in specific cases. The thesis is divided into six chapters. The first chapter provides the definition of a negative. In this chapter the author points out potential problems linked to the identification of a negative, especially to the difference between a fact, a negative statement and a legal assessment. The second one addresses the possibility of a negative becoming the subject of proving as a practical way of finding of facts. The chapter also brings a brief analysis of proving a negative abroad, specifically in Slovakia, the Great Britain and the United States of America. The third, the fourth and the fifth chapter deal with the subject of proving negatives in civil proceedings, administrative proceedings and criminal proceedings respectively. In each of these chapters the author summarises the principles of proving related to corresponding type of proceedings and demonstrates proving negatives on specific cases (regarding...

Aerobic Gram negative bacteria such as Pseudomonas putida were reported to possess class A ATCases and to have a M.W. of 360 kD. The nucleotide sequence of the P. putida pyrBC was determined to answer this question once and for all. The expected regulatory gene was not found. It is shown that the P. putida pyrB gene is overlapped by pyrC by 4 bp. The P.putida pyrB is 1005 bp (335 aa) in length and the pyrC is 1275 bp (425 aa) long. Both of these genes complement E. coli mutants with their respective genotypes. Another finding borne out from the sequence is an effector binding site at the N-terminus of pyrB of P. putIda. The binding site shows that effectors compete with carbamoylphosphate for the active site. In this dissertation, it is shown that the ATCase of P.putida is a trimer of M.W. of 109 kD (3 x 36.4 kD) and that the gene encoding pyrB is overlapped by the pyrC gene which encodes DHOase. It is also shown that the pyrBC encoded enzymes copurify as a dodecameric complex with a M.W. of 484 kD.

Social media services are used by around 60% of the world's population and the use is growing. Studies have shown that social media have both positive and negative effects on users and as social media is used by such a large part of the population and is used within many aspects of society it can have a very high impact on society in general. That is why it is important to try to reduce the negative aspects of social media that individuals experience so that those negative aspects do not transfer from the service to society in general.  The aim of this study is to investigate both positive and negative aspects individuals experience when using Facebook and then take a deeper look at what is creating the negative experiences. The goal is to be able to find possible ways to change the aspects that create negative experiences to something less negative or positive instead.  To achieve the purpose and goal of this study a survey was sent out and follow up interviews were held with individuals to clarify what the positive and negative aspects were. In the interviews this study took a more detailed look at what is creating the negative experiences and how certain social media aspects might be changed to create less negative or more positive experiences.  The result concludes that the individuals that were interviewed indeed believe there are ways to change the aspects in social media that currently create negative experiences to something that creates more positive experiences. Some of those are using AI, giving users more control or transparency into the service they use and new ways to create trust between the users and the platform they are using. These results can be used by developers to create better platforms and services with less negative experiences for users.

Background: Blastomycosis is caused by a dimorphic fungus that can be difficult to diagnose in certain situations. The disease is sometimes serious and can be deadly. Diagnosis by fungal serology and urinary antigens is not easy to establish and unreliable. Culture is also time-consuming and is not easy to perform. Thus, documentation of such an organism on cytology offers a quick and cost-effective alternative. This report describes for the first time identification of the 'negative image' of Blastomyces budding yeast. Case: A 79-year-old man presented with a left lung nodule associated with mediastinal and hilar lymphadenopathy. Fine needle aspiration was performed, and a 'negative image' of a yeast with wide base budding was noted on Diff-Quik (DQ)-stained smears. Blastomyces species were confirmed with periodic acid-Schiff fungal stain. Additionally, the fungal capsule contained focally polarizable material on Congo red stain and lacked mucin with mucicarmine stain. Conclusion: Blastomyces yeast forms can be easily identified with DQ staining by their 'negative image'. This feature can be utilized as a quick and cost-effective cytological characteristic to further triage these specimens for confirmation. The information can be of great value to clinicians in making appropriate clinical decisions.

Background: Individuals experiencing negative and potentially traumatic life events are at increased risk for suicidal thoughts and behaviors; however, suicidal outcomes are not inevitable. Individuals who attribute negative life events to external, transient, and specific factors, rather than internal, stable, and global self-characteristics, may experience fewer deleterious outcomes, including suicidal behavior. Aims: This study examines the moderating effect of explanatory style on the relationship between negative life experiences and suicide ideation in a college student sample. Methods: A total of 138 participants (73% female) were recruited from a rural. Eastern college and completed a self-report psychosocial assessment. Results: Optimistic explanatory style mitigates the influence of negative and potentially traumatic life events on thoughts of suicide, above and beyond the effects of hopelessness and depression. Conclusions: Beliefs about the origin, pervasiveness, and potential recurrence of a negative life event may affect psychological outcomes. Optimistic explanatory style was associated with reduced suicide ideation, whereas pessimistic explanatory style was associated with increased thoughts of suicide. Optimistic reframing of negative life events for clients may have treatment implications for the prevention of suicidal activity.