SUMMARY Temporary and permanent shells are found in nature which transform with a growing organism and change their form while the organism develops or they are get rid off and grow up again thgrough the living cycle they serve as means of protection. In some cases, we can compare shells with person‘s emotinion states. Individ‘s innerworld, in which, he for some reasons closes in, we can call „shelter“ – he closes in willingly, in other cases the shell might be thrusted by other subject, or by other psychologically or physically stronger individs. Willinglly closed individ, is followed by such subjects as: closeness, not communicating, not improving, torpidity, separation from the world, from inovation. Individ closes in himself, separates from society, feel push off, misundertood. Either society is closed for individual for his not knowing, unwill to understand, to accept him the way he is, for losing relations, for interests missunderstanding, for difference of psichical possibilities. This is a topical issue which excites the minds of not only ordinary people but also artists, sociologist and scientists.

SUMMARY The master‘s thesis titled “Logic of Labyrinths and Alogisms” seeks to disclose the meaning of the labyrinth symbol and to go deep into it as a phenomenon existing in reality. The non-heterogeneity of this phenomenon has been disclosed by analysing the symbol of the labyrinth through the psychological, philosophical and mythological prisms. The author has made a conclusion that the labyrinth has two spheres of existence, which may be conditionally subdivided into logical and alogical. The author aimed at expressing the main concept of the theoretical studies of the master‘s thesis by using the artistic means. The principal aim was not only to maintain the classical depiction of the labyrinth but also to convey the phenomenon through the personal prism. Although the leather art employs a number of techniques and materials, the classical techniques and materials have been chosen in this master‘s thesis. Various art technologies of leather work have been paralleled in this creative project keeping to natural leather as a principal material. The analysis has come to the conclusion that the creative work of any artist may be compared with the search of the keys that could open the locks of sub-consciousness. During the creative process and with every new work the artist as if opens the lock on one of the doors that keep some secret behind them.

Methods for assessing small peripheral nerve fiber function objectively are limited. The cutaneous silent period (CuSP), a transient supression of electromyographic voluntary activity that follows painful stimuli, could serve as an objective functional measure of the A delta fibers. The aim of this study was to evaluate function of small diameter A delta nerve fibers using the CuSP in normals and in patients with both focal (carpal tunnel syndrome - CTS) and generalised (polyneuropathies - PNP) peripheral nerve injuries. The objectives of the study were the following: 1. To determine the CuSP normal values by age, gender, side, height and weight in healthy controls. 2. To study the topography and extension of the CuSP by changing recording and stimulating sites in healthy controls. 3. To evaluate the CuSP in patients with CTS of different severity and to compare these results with those of healthy controls. 4. To estimate the CuSP onset latency and duration in relation with median nerve evoked compound muscle action potential amplitude, distal latency and conduction velocity in patients with CTS. 5. To evaluate the CuSP in patients with PNP of different types and to compare these results with those of healthy controls. 6. To estimate the CuSP onset latency and duration in relation with median nerve evoked compound muscle action potential amplitude, distal latency and conduction velocity in patients with PNP. We investigated the CuSP of the upper and lower limbs of 50 normal... [to full text]

The master’s thesis titled “Book – Letter to Mankind” may be characterised as having an unexpected solution of depicting a letter. The collection comprises various art techniques of leather work including the author’s technique. The works are distinguished by their novelty, the delicate thematic solution and the application of aesthetic qualities of used materials. The principal object of the collection is the book as a letter bearing its own history, the interrelation between a man and a letter and an attempt to express this idea within the creative activity. It is a three-piece collection aiming at the development of the main idea “Book – Letter to Mankind”. The collection of works was performed with the reference to the trends of Conceptualism. The used materials include natural leather, pasteboard, textile, blocks of old books and few auxiliary materials. The reference was made to the works of conceptual art as well. After the analysis of the topic through the creative process and theoretical research the author of this master’s thesis came to the conclusion that any object bearing some information of mankind could be qualified as a letter.

Stiffnes of leg's receiver and skin and soft tissues at contact place are researched in the master thesis. Using the finite elements method with digital ANSYS programmable packet was made non-linear model of leg's prothesis receiver and stump of soft tissues . Made calculiation have shown that deformation ratio in stump and receiver contact declines when the lood increases. Therefore it is very important to evaluate not only the height of desabled persion but also his weight durind estimation the magnitude of deviations of leg prosthesis manufacturing and assembly.

Teoriniame darbe yra nagrinėjama žalčio semantinė prasmė, jo kultas Lietuvoje, įvaizdis tautodailėje ir tautosakoje, taip pat apžvelgiami žalčio motyvai šiuolaikiniame mene. Senųjų tikėjimų ir pasaulėjautos atmintis tebėra kiekvieno lietuvio sielos dalis. Kas paveldėta iš gilios senovės, neišnyksta be pėdsakų. Bėgant metamas, religijos ir socialinės struktūros keičiasi, daugelis mitinių būtybių bei simbolių išlieka transformuota forma. Skaitant įvairius žurnalistinius straipsnius, stebint šiandieninę visuomenę, pastebimas žmonių pasimetimas tarp daugybės informacijos, pasiūlymų, patarimų, kaip gyventi, kaip mąstyti, ką valgyti ir t.t. Tokiame sraute informacijos žmogus natūraliai pradeda abejoti, ar verta laikytis tradicijų, papročių, ar verta tikėti, pasitikėti. / In theoretical work is analyzed serpent's semantic meaning, his cult in Lithuania, serpent's image in folk art and folklore, also his motifs in contemporary art. The old beliefs and worldview memory is still part of the soul of every Lithuanian. Religion and social structure is changing over the years, many mythical beings and symbols remain transformed forms.Reading journalistic articles, monitoring today's society is noticeable human’s confusion in a lot of kinds of information, suggestions and tips how to live, how to think, what to eat and so on. I With these leather wall composition I am trying to show certain direction, which helps to bring back faith in certain values, which do not sacrifice your beliefs and remain confident.

Acceleration of the regeneration process is necessary for severe full-thickness skin injuries, such as burns, trauma or chronic ulcers. The aim of this dissertation was to investigate the influence of bone marrow-derived stem cells on skin regeneration using full-thickness wound model in BALB/c mouse. Lin¯ cells were isolated and identified. Regenerative properties of purified cells were evaluated in vitro. Wound healing after Lin¯ cells transplantation was examined histologically and cytokine gene expression was determined by quantitative RT-PCR. The results revealed that Lin¯ population is heterogeneous and contains distinct undifferentiated cell types. In vitro experiments showed that skin tissue extracellular matrix components enhance proliferation and migration of Lin¯ cells. Histologic analysis indicated that inhibition of inflammation, re-epithelization, formation of skin appendages and extracellular collagen, were most effective after cell transplantation with type I collagen. Quantitative analysis revealed that transplanted Lin¯ cells decrease expression of proinflammatory cytokine TNF-α and increase anti-inflammatory IL-10 expression. Also, gene expression of growth factors TGF-β and VEGF, which enhance regeneration, was improved in different healing phases. The results obtained provide additional knowledge for possible therapeutic application of stem/progenitor cells in difficult-to-heal wounds. / Odos žaizdų regeneracijos skatinimas yra aktualus pažeidus didelį paviršiaus plotą bei giliuosius audinio sluoksnius. Šio darbo tikslas buvo ištirti kaulų čiulpų Lin¯ ląstelių įtaką odos regeneracijai in vivo, taikant BALB/c linijos pelių visų odos sluoksnių pažeidimo modelį. Darbo metu buvo išskirtos bei identifikuotos Lin¯ ląstelės. Jų regeneracinės savybės buvo įvertintos in vitro. Buvo atlikta histologinė gyjančio audinio analizė bei nustatyti citokinų genų raiškos pokyčiai. Rezultatai parodė, kad Lin¯ ląstelių populiacija yra heterogeniška, sudaryta iš nediferencijuotų ląstelių. Nustatyta, kad odos tarpląstelinės medžiagos komponentai skatina Lin¯ ląstelių proliferaciją ir migraciją in vitro. Histologinė analizė parodė, kad po ląstelių transplantacijos I tipo kolageno gelyje anksčiausiai prasideda uždegimo slopinimas, greičiausiai baigiamas reepitelizacijos procesas, atsinaujinusioje dermoje susiformuoja tvarkingai išsidėsčiusios pagalbinės odos struktūros bei natyviam audiniui būdingas tarpląstelinės medžiagos kolagenas. Atlikus kiekybinę analizę nustatyta, kad po Lin¯ ląstelių transplantacijos sumažėja uždegiminio citokino TNF-α raiška bei padidėja priešuždegiminio IL-10 raiška. Skirtingose gijimo stadijose padidėja augimo veiksnių, skatinančių audinio regeneraciją, TGF-β ir VEGF genų raiška. Gauti rezultatai papildo žinias, reikalingas vystyti metodus, skirtus sutrikusio gijimo žaizdų regeneracijos skatinimui.

Magistrinio darbo pavadinimas: - “Šunų odos bakterinių ligų sukėlėjų išskyrimas ir jautrumo antimikrobinėms medžiagoms nustatymas““ Autorius: Jogaila Šlekys Magistrinio darbo vadovas: Prof. dr. Jūratė Šiugždaitė Darbo tikslas: Išskirti mikroorganizmus iš šunų odos ir nustatyti jautrumą antimikrobinėms medžiagoms. Rezultatai: Iš 60 tirtų šunų odos mėginių 15 (25 proc.) buvo identifikuoti Staphylococcus aureus mikroorganizmai. 10-tyje mėginių – Streptococcus spp. (16.5 proc.) mikroorganizmai ir 25-se atvejuose (42 proc.) buvo identifikuoti Staphylococcus intermedius mikroorganizmai. 10-tyje mėginių (16,5 proc.) mikroorganizmai neišaugo. Mes nustatėme, kad šunys nuo 1 iki 5 metų amžiaus sirgo daugiausiai (64 proc.). Iš visų tyrime dalyvavusių šunų, sirgusių odos bakterinėmis ligomis , (52 proc.) buvo patelės ir (48 proc.) patinai. Staphylococcus intermedius ir Staphylococcus aureus iškyrimui įtakos turėjo: laikymo sąlygos ir amžius. Sezoniškumas įtakos turėjo Staphylococcus intermedius išskyrimui. Atlikus tyrimus nustatėme, kad Staphylococcus aureus padermės jautriausios buvo ampicilinui (90%) ir cefaleksinui (89%). Staphylococcus intermedius jautriausi buvo cefaleksinui (90 proc.) ir cefovecinui (80 proc.). Streptococcus spp. mikroorganizmai jautriausi buvo tetraciklinui (100 proc.) ir trimetoprimui (100 proc.). Labiausiai mikroorganizmai buvo atsparūs amoksicilinui (100 proc.). Išvados: 1. Atlikus tyrimus nustatėme, kad vyraujantys šunų odos bakterinių ligų sukėlėjai yra... [toliau žr. visą tekstą] / Master final assignment: - “Isolation of dogs bacterial skin disease pathogens and determination of antimicrobial susceptibility“ Author: Jogaila Šlekys Tutor: Prof. dr. Jūratė Šiugždaitė Aim of the study: To evaluate pathogens from canine bacterial skin diseases and determine antimicrobial susceptibility. Results: 15 (25 %) out of 60 cases, where skin smears were taken from domestic animals, pathogenic strains of Staphylococcus aureus were observed, in 10 cases – Streptococcus spp. (16.5%) microorgarnisms and in 25 cases (42%) were determined Staphylococcus intermedius. In 10 samples (16,5%) strains of microorganisms didn‘t occur. We determined, that canine from 1 to 5 years old were mostly affected (64%). We determined that among all of investigated animals morbidity of skin bacterial diseases in females were 52% and in males 48%. Staphylococcus intermedius and Staphylococcus aureus isolation influenced by: canine housing conditions and age. Seasonality affected the Staphylococcus intermedius isolation. During research it was seen that strains of Staphylococcus aureus were most sensitive for Ampicillin (90%) and Cephalexin (89%). Staphylococcus intermedius were most sensitive for Cephalexin (90%) and Cefovecin (80%). Strains of Streptococcus spp. microorganisms mostly showed sensitivity for Tetracycline (100%) and Trimethoprim (100%). Gentamicin was also effective – (75%). Most microorganisms were Amoxicillin resistant (100%). Conclusion: 1. During the research it was... [to full text]

The aim of the study was to evaluate the expression of immunosuppressive (FOXP3, NKG2A), and cytotoxic (perforin) or cytotoxic / immunomodulating (IFNγ) T-cell properties representing biomarkers in the peripheral blood CD8h T-cell population of patients with advanced renal cell carcinoma (RCC) or high risk cutaneous melanoma and healthy controls by multicolour flow cytometry. Determination of the percentage of functionally competing T-cell subsets (especially immunosuppressive) in the CD8hCD57+ T-cell subpopulation in future may serve as one of parameters enabling to assess the overall status of antitumor immune response and select cancer patients most suitable for antitumor immunotherapy while dismissing those to whom it would be ineffective or even harmful. / Darbo tikslas buvo įvertinti imunosupresines (FOXP3, NKG2A), citotoksines (perforin) bei citotoksines / imunomoduliuojančias (IFNγ) savybes atspindinčių žymenų raiškos skirtumus išplitusiu inkstų vėžiu ar didelės rizikos odos melanoma sergančių pacientų periferinio kraujo CD8h T limfocitų populiacijoje, lyginant su kontroline grupe. Skirtingas T limfocitų savybes atspindinčių žymenų raiška buvo tiriama tėkmės citometrijos būdu. Nustatyta, kad inkstų vėžiu ar odos melanoma sergančių pacientų periferiniame kraujyje Įvairių subpopuliacijų (ypač imunosupresinės) nuošimčio nustatymas CD8hCD57+ T limfocitų populiacijoje ateityje gali būti naudingas klinikinėje praktikoje, individualizuojant priešnavikinę imunoterapiją ir selektyviai parenkant tik tuos pacientus, kuriems imuninės sistemos aktyvinimas sukeltų navikinių ląstelių naikinimą, o ne dar labiau gilintų imunosupresiją.

Darbo tikslas buvo įvertinti imunosupresines (FOXP3, NKG2A), citotoksines (perforin) bei citotoksines / imunomoduliuojančias (IFNγ) savybes atspindinčių žymenų raiškos skirtumus išplitusiu inkstų vėžiu ar didelės rizikos odos melanoma sergančių pacientų periferinio kraujo CD8h T limfocitų populiacijoje, lyginant su kontroline grupe. Skirtingas T limfocitų savybes atspindinčių žymenų raiška buvo tiriama tėkmės citometrijos būdu. Nustatyta, kad inkstų vėžiu ar odos melanoma sergančių pacientų periferiniame kraujyje Įvairių subpopuliacijų (ypač imunosupresinės) nuošimčio nustatymas CD8hCD57+ T limfocitų populiacijoje ateityje gali būti naudingas klinikinėje praktikoje, individualizuojant priešnavikinę imunoterapiją ir selektyviai parenkant tik tuos pacientus, kuriems imuninės sistemos aktyvinimas sukeltų navikinių ląstelių naikinimą, o ne dar labiau gilintų imunosupresiją. / The aim of the study was to evaluate the expression of immunosuppressive (FOXP3, NKG2A), and cytotoxic (perforin) or cytotoxic / immunomodulating (IFNγ) T-cell properties representing biomarkers in the peripheral blood CD8h T-cell population of patients with advanced renal cell carcinoma (RCC) or high risk cutaneous melanoma and healthy controls by multicolour flow cytometry. Determination of the percentage of functionally competing T-cell subsets (especially immunosuppressive) in the CD8hCD57+ T-cell subpopulation in future may serve as one of parameters enabling to assess the overall status of antitumor immune response and select cancer patients most suitable for antitumor immunotherapy while dismissing those to whom it would be ineffective or even harmful.