No description available.

Boron neutron capture therapy (BNCT) has the potential to specifically destroy tumor cells without damaging the tissues infiltrated by the tumor. BNCT is a binary treatment method based on the combination of two agents that have no effect when applied individually: 10B and thermal neutrons. Exclusively, the combination of both produces an effect, whose extent depends on the amount of 10B in the tumor but also on the organs at risk. It is not yet possible to determine the 10B concentration in a specific tissue using non-invasive methods. At present, it is only possible to measure the 10B concentration in blood and to estimate the boron concentration in tissues based on the assumption that there is a fixed uptake of 10B from the blood into tissues. On this imprecise assumption, BNCT can hardly be developed further. A therapeutic approach, combining the boron carrier for therapeutic purposes with an imaging tool, might allow us to determine the 10B concentration in a specific tissue using a non-invasive method. This review provides an overview of the current clinical protocols and preclinical experiments and results on how innovative drug development for boron delivery systems can also incorporate concurrent imaging. The last section focuses on the importance of proteomics for further optimization of BNCT, a highly precise and personalized therapeutic approach.

No description available.

Near field imaging using microwave in medical applications has gain much attention recently as various researches show its high ability and accuracy in illuminating object comparing to the well-known screening tools such as Magnetic Resonance Imaging (MRI), digital mammography, ultrasound etc. This has encourage and motivate scientists continue to exploit the potential of microwave imaging so that a better and more powerful sensing tools can be developed. This thesis documents the development of antenna design for microwave imaging application such as breast cancer detection. The application is similar to the concept of Ground Penetrating Radar (GPR) but operating at higher frequency band. In these systems a short pulse is transmitted from an antenna to the medium and the backscattered response is investigated for diagnose. In order to accommodate such a short pulse, a very wideband antenna with a minimal internal reflection is required. Printed monopole and planar metal plate antenna is implemented to achieve the necessary operating wide bandwidth. The development of new compact printed planar metal plate ultra wide bandwidth antenna is presented. A generalized parametric study is carried out using two well-known software packages to achieve optimum antenna performance. The Prototype antennas are tested and analysed experimentally, in which a reasonable agreement was achieved with the simulations. The antennas present an excellent relative wide bandwidth of 67% with acceptable range of power gain between 3.5 to 7 dBi. A new compact size air-dielectric microstrip patch-antenna designs proposed for breast cancer detection are presented. The antennas consist of a radiating patch mounted on two vertical plates, fed by coaxial cable. The antennas show a wide bandwidth that were verified by the simulations and also confirmed experimentally. The prototype antennas show excellent performance in terms the input impedance and radiation performance over the target range bandwidth from 4 GHz to 8 GHz. A mono-static model with a homogeneous dielectric box having similar properties to human tissue is used to study the interaction of the antenna with tissue. The numerical results in terms the matching required of new optimised antennas were promising. An experimental setup of sensor array for early-stage breast-cancer detection is developed. The arrangement of two elements separated by short distance that confined equivalent medium of breast tissues were modelled and implemented. The operation performances due to several orientations of the antennas locations were performed to determine the sensitivity limits with and without small size equivalent cancer cells model. In addition, a resistively loaded bow tie antenna, intended for applications in breast cancer detection, is adaptively modified through modelling and genetic optimisation is presented. The required wideband operating characteristic is achieved through manipulating the resistive loading of the antenna structure, the number of wires, and their angular separation within the equivalent wire assembly. The results show an acceptable impedance bandwidth of 100.75 %, with a VSWR < 2, over the interval from 3.3 GHz to 10.0 GHz. Feasibility studies were made on the antenna sensitivity for operation in a tissue equivalent dielectric medium. The simulated and measured results are all in close agreement.

Within the archaeological record ‘mounds’ are often ubiquitous. They are common in many ancient cultures, and they vary in size, construction techniques and use. This research is focused upon optimizing the use of remote sensing for the non-invasive study of mounds both in the United States and the United Kingdom. This thesis presents three representative earthen mound sites and proposes a comprehensive and modular survey methodology to guide the planning and execution of a mound survey tailored to the unique requirements presented by the cultural resource at a particular location. In doing so, the research has provided optimized approaches to high resolution three-dimensional topographic models using a variety of digital methods. These models have been shown to accurately capture the variability of the modern ground surface, which is of vital importance to the management of the mounds. Furthermore, these models have proved vital for integrating geophysical methods into the holistic workspace, thereby providing a better archaeological understanding of the below ground remains. Every mound surveyed presented different challenges, and therefore had to be approached in a slightly different way. However, the general methodology was highly effective for both characterizing below-ground archaeological and natural anomalies, and for assessing the state of preservation of all mounds surveyed. As a result, a flowchart has been generated for non-invasive assessment of mounds in general. If followed, this will allow the production of a “snapshot” of the mound or mound group at a fixed point in time with the resolution necessary to produce useful and insightful interpretation. While this research focuses on the application of geophysical and topographic survey in the United Kingdom and United States to a mound or mound group, this methodology and the associated outcomes can be valuable more globally not only for archaeology, but also heritage management.

No description available.

<p>We evaluated 3-D imaging of coarse root structure and biomass using ground-penetrating radar (GPR). GPR surveys were conducted in a white pine forest in southern Ontario, Canada. GPR profiles were obtained across two test plots (6 and 17 m² area), using 1-GHz GPR and a MEMS (micro-electro-mechanical systems) accelerometer. Test plot surveys evaluated the effects of micro-topography, soil moisture content, and root diameter and spacing. In addition, with the aid of the outcome of the control test plots two other plots (25 and 400 m²area) were surveyed with varying line sample spacing to investigate the restraints on resolution brought about by line sampling density.</p> <p>Accounting for antenna tilt is necessary to determine an accurate and more precise position of root mass. The antenna tilt was >45^o pitch, >28^o roll and up to 10^o yaw due to surface micro-topography of the forest floor. Vector 3-D imaging enhanced the diffraction amplitude (15.5% increase) and centralized the position of the root. Radial surveys provided root continuity and produced better root imaging.</p> <p>GPR largely underestimates coarse root biomass when a line spacing of 25 cm is used. However similar results are found with smaller line spacing (12.5 cm). A maximum line spacing of 10 cm provided continuous root structure and differentiation of roots spaced 10 cm apart and greater. A sampling line spacing of 5 cm and an inline sampling interval of 0.5 cm in low soil moisture conditions provided the detection of roots that were a minimum of 1.4 cm in diameter.</p> / Master of Science (MSc)

[EN] Over time, due to multiple operational and maintenance activities, the networks of water supply systems (WSSs) undergo interventions, modifications or even are closed. In many cases, these activities are not properly registered. Knowledge of the paths and characteristics (status and age, etc.) of the WSS pipes is obviously necessary for efficient and dynamic management of such systems. This problem is greatly augmented by considering the detection and control of leaks. Access to reliable leakage information is a complex task. In many cases, leaks are detected when the damage is already considerable, which brings high social and economic costs. In this sense, non-destructive methods (e.g., ground penetrating radar - GPR) may be a constructive response to these problems, since they allow, as evidenced in this thesis, to ascertain paths of pipes, identify component characteristics, and detect primordial water leaks. Selection of GPR in this work is justified by its characteristics as non-destructive technique that allows studying both metallic and non-metallic objects. Although the capture of information with GPR is usually successful, such aspects as the capture settings, the large volume of generated information, and the use and interpretation of such information require high level of skill and experience. This dissertation may be seen as a step forward towards the development of tools able to tackle the problem of lack of knowledge on the WSS buried assets. The main objective of this doctoral work is thus to generate tools and assess their feasibility of application to the characterization of components of WSSs from GPR images. In this work we have carried out laboratory tests specifically designed to propose, develop and evaluate methods for the characterization of the WSS buried components. Additionally, we have conducted field tests, which have enabled us to determine the feasibility of implementing such methodologies under uncontrolled conditions. The methodologies developed are based on techniques of intelligent data analysis. The basic principle of this work has involved the processing of data obtained through the GPR to look for useful information about WSS components, with special emphasis on the pipes. After performing numerous activities, one can conclude that, using GPR images, it is feasible to obtain more information than the typical identification of hyperbolae currently performed. In addition, this information can be observed directly, e.g. more simply, using the methodologies proposed in this doctoral work. These methodologies also prove that it is feasible to identify patterns (especially with the preprocessing algorithm termed Agent race) that provide fairly good approximation of the location of leaks in WSSs. Also, in the case of pipes, one can obtain such other characteristics as diameter and material. The main outcomes of this thesis consist in a series of tools we have developed to locate, identify and visualize WSS components from GPR images. Most interestingly, the data are synthesized and reduced so that the characteristics of the different components of the images recorded in GPR are preserved. The ultimate goal is that the developed tools facilitate decision-making in the technical management of WSSs, and that such tools can even be operated by personnel with limited experience in handling non-destructive methodologies, specifically GPR. / [ES] Con el paso del tiempo, y debido a múltiples actividades operacionales y de mantenimiento, las redes de los sistemas de abastecimiento de agua (SAAs) sufren intervenciones, modificaciones o incluso, son clausuradas, sin que, en muchos casos, estas actividades sean correctamente registradas. El conocimiento de los trazados y características (estado y edad, entre otros) de las tuberías en los SAAs es obviamente necesario para una gestión eficiente y dinámica de tales sistemas. A esta problemática se suma la detección y el control de las fugas de agua. El acceso a información fiable sobre las fugas es una tarea compleja. En muchos casos, las fugas son detectadas cuando los daños en la red son ya considerables, lo que trae consigo altos costes sociales y económicos. En este sentido, los métodos no destructivos (por ejemplo, ground penetrating radar - GPR), pueden ser una respuesta a estas problemáticas, ya que permiten, como se pone de manifiesto en esta tesis, localizar los trazados de las tuberías, identificar características de los componentes y detectar las fugas de agua cuando aún no son significativas. La selección del GPR, en este trabajo se justifica por sus características como técnica no destructiva, que permite estudiar tanto objetos metálicos como no metálicos. Aunque la captura de información con GPR suele ser exitosa, la configuración de la captura, el gran volumen de información, y el uso y la interpretación de la información requieren de alto nivel de habilidad y experiencia por parte del personal. Esta tesis doctoral se plantea como un avance hacia el desarrollo de herramientas que permitan responder a la problemática del desconocimiento de los activos enterrados de los SAAs. El objetivo principal de este trabajo doctoral es, pues, generar herramientas y evaluar la viabilidad de su aplicación en la caracterización de componentes de un SAA, a partir de imágenes GPR. En este trabajo hemos realizado ensayos de laboratorio específicamente diseñados para plantear, elaborar y evaluar metodologías para la caracterización de los componentes enterrados de los SAAs. Adicionalmente, hemos realizado ensayos de campo, que han permitido determinar la viabilidad de aplicación de tales metodologías bajo condiciones no controladas. Las metodologías elaboradas están basadas en técnicas de análisis inteligentes de datos. El principio básico de este trabajo ha consistido en el tratamiento adecuado de los datos obtenidos mediante el GPR, a fin de buscar información de utilidad para los SAAs respecto a sus componentes, con especial énfasis en las tuberías. Tras la realización de múltiples actividades, se puede concluir que es viable obtener más información de las imágenes de GPR que la que actualmente se obtiene con la típica identificación de hipérbolas. Esta información, además, puede ser observada directamente, de manera más sencilla, mediante las metodologías planteadas en este trabajo doctoral. Con estas metodologías se ha probado que también es viable la identificación de patrones (especialmente el pre-procesado con el algoritmo Agent race) que proporcionan aproximación bastante acertada de la localización de las fugas de agua en los SAAs. También, en el caso de las tuberías, se puede obtener otro tipo de características tales como el diámetro y el material. Como resultado de esta tesis se han desarrollado una serie de herramientas que permiten visualizar, identificar y localizar componentes de los SAAs a partir de imágenes de GPR. El resultado más interesante es que los resultados obtenidos son sintetizados y reducidos de manera que preservan las características de los diferentes componentes registrados en las imágenes de GPR. El objetivo último es que las herramientas desarrolladas faciliten la toma de decisiones en la gestión técnica de los SAAs y que tales herramientas puedan ser operadas incluso por personal con una experiencia limitada en el manejo / [CA] Amb el temps, a causa de les múltiples activitats d'operació i manteniment, les xarxes de sistemes d'abastament d'aigua (SAAs) se sotmeten a intervencions, modificacions o fins i tot estan tancades. En molts casos, aquestes activitats no estan degudament registrats. El coneixement dels camins i característiques (estat i edat, etc.) de les canonades d'aigua i sanejament fa evident la necessitat d'una gestió eficient i dinàmica d'aquests sistemes. Aquest problema es veu augmentat en gran mesura tenint en compte la detecció i control de fuites. L'accés a informació fiable sobre les fuites és una tasca complexa. En molts casos, les fugues es detecten quan el dany ja és considerable, el que porta costos socials i econòmics. En aquest sentit, els mètodes no destructius (per exemple, ground penetrating radar - GPR) poden ser una resposta constructiva a aquests problemes, ja que permeten, com s'evidencia en aquesta tesi, per determinar rutes de canonades, identificar les característiques dels components, i detectar les fuites d'aigua quan encara no són significatives. La selecció del GPR en aquest treball es justifica per les seves característiques com a tècnica no destructiva que permet estudiar tant objectes metàl·lics i no metàl·lics. Tot i que la captura d'informació amb GPR sol ser reeixida, aspectes com ara la configuració de captura, el gran volum d'informació que es genera, i l'ús i la interpretació d'aquesta informació requereix alt nivell d'habilitat i experiència. Aquesta tesi pot ser vista com un pas endavant cap al desenvolupament d'eines capaces d'abordar el problema de la manca de coneixement sobre els actius d'aigua i sanejament enterrat. L'objectiu principal d'aquest treball doctoral és, doncs, generar eines i avaluar la seva factibilitat d'aplicació a la caracterització dels components de los SAAs, a partir d'imatges GPR. En aquest treball s'han dut a terme proves de laboratori específicament dissenyats per proposar, desenvolupar i avaluar mètodes per a la caracterització dels components d'aigua i sanejament soterrat. A més, hem dut a terme proves de camp, que ens han permès determinar la viabilitat de la implementació d'aquestes metodologies en condicions no controlades. Les metodologies desenvolupades es basen en tècniques d'anàlisi intel·ligent de dades. El principi bàsic d'aquest treball ha consistit en el tractament de dades obtingudes a través del GPR per buscar informació útil sobre els components d'SAA, amb especial èmfasi en la canonades. Després de realitzar nombroses activitats, es pot concloure que, amb l'ús d'imatges de GPR, és factible obtenir més informació que la identificació típica d'hipèrboles realitzat actualment. A més, aquesta informació pot ser observada directament, per exemple, més simplement, utilitzant les metodologies proposades en aquest treball doctoral. Aquestes metodologies també demostren que és factible per identificar patrons (especialment el pre-processat amb l'algoritme Agent race) que proporcionen bastant bona aproximació de la localització de fuites en SAAs. També, en el cas de tubs, es pot obtenir altres característiques com ara el diàmetre i el material. Els principals resultats d'aquesta tesi consisteixen en una sèrie d'eines que hem desenvolupat per localitzar, identificar i visualitzar els components dels SAAS a partir d'imatges GPR. El resultat més interessant és que els resultats obtinguts són sintetitzats i reduïts de manera que preserven les característiques dels diferents components registrats en les imatges de GPR. L'objectiu final és que les eines desenvolupades faciliten la presa de decisions en la gestió tècnica de SAA, i que tals eines poden fins i tot ser operades per personal amb poca experiència en el maneig de metodologies no destructives, específicament GPR. / Ayala Cabrera, D. (2015). Characterization of components of water supply systems from GPR images and tools of intelligent data analysis [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/59235 / Premios Extraordinarios de tesis doctorales

This thesis investigates the application of In-Situ Recycling and provides guidelines for localities to aid in the selection of recycling methods, as well as documents a local government's experience with Cold In-Place Recycling. The recycling methods discussed in this study include Cold In-Place Recycling (CIR), Hot In-Place Recycling (HIR), and Full Depth Reclamation (FDR). These methods are performed onsite and in-place in a continuous process of milling, mixing, and placement. The In-Situ Recycling guidelines include suggestions based on: traffic characteristics, existing road condition, distress types, road access, local climate, road geometry, and other road characteristics. The guidelines are based on information from sources including NCHRP Synthesis 421, American Recycling and Reclamation Association (ARRA), FHWA, and state agencies with recycling experience. This study also resulted in documenting obstacles that localities may face when in-situ recycling, as well as the impact of limited experience with recycling. The study also evaluated the construction of Cold In-Place Recycled pavement sections in Christiansburg, VA, using Falling Weight Deflectometer (FWD) and Ground Penetrating Radar (GPR). Additionally, using the FWD and GPR data, alternate recycled designs were proposed in addition to a cost comparison to a conventional design. / Master of Science

Limitierend für pharmakologische Therapien ist oft die Unfähigkeit des Wirkstoffes, biologische Membranen zu überwinden, weswegen häufig Transportmoleküle wie z.B. zellpenetrierende Peptide (CPPs, cell penetrating peptides) benutzt werden. Von den über 100 beschriebenen CPPs wurde bisher nur eine kleine Anzahl systematisch verglichen, was die Auswahl des „richtigen“ CPPs für eine Anwendung erschwert. Ziel dieser vorliegenden Arbeit war es, das pro-apoptotische Peptid KLA mittels CPPs spezifisch in Krebszellen zu transportieren. Untersucht wurden: (I) Verschiedene CPPs in unterschiedlichen Zelltypen zur Selektion der „besten“ CPPs; (II) Der Einflusses des CPP C-Terminus auf die Internalisierung und zelluläre Verteilung; (III) Der zelluläre KLA-Transport mittels CPPs via einer nicht-kovalenten Administration. 22 verschiedene CPPs wurden in sieben Zelltypen untersucht, wobei Toxizität, Zellaufnahme und zelluläre Lokalisation mittels Fluorescein markierten CPPs in Fluoreszenzspektroskopie und konfokaler Mikroskopie betrachtet wurden. Abhängig von der Zellaufnahme wurden die CPPs in drei Gruppen klassifiziert. Die Untersuchung carboxylierter und carboxyamidierter CPP C-Termini ergab, dass in den meisten Fällen ein Carboxyamid die zelluläre Aufnahme begünstigte. Drei CPPs (MPG, Penetratin und Integrin) wurden ausgewählt, um das pro-apoptotische KLA Peptid in zwei Krebszelllinien (MCF-7 Brustkrebszellen und leukämische RAW264.7 Makrophagen) im Vergleich zu Fibroblasten (Cos-7) nicht-kovalent zu transportieren. Der erfolgreiche KLA-Transport hing vom CPP, dessen C-Terminus und der Zelllinie ab. Die Analyse der Viabilität nach CPP:KLA Administration ergab, dass MPG-CONH2:KLA (1:2) toxisch für Makrophagen und Brustkrebszellen, aber nicht für Fibroblasten war. Die Toxizität konnte der Apoptose zugeordnet werden. Die vorliegende Arbeit liefert wichtige Informationen über die Auswahl des passenden CPPs für den nicht-kovalenten Transport des pro-apoptotischen KLA-Peptids. / Limitations in a pharmacological therapy are often due to the inability of drugs to overcome the cell membrane and therefore transporting molecules are being used, e.g. cell penetrating peptides (CPPs). Only a few of the over 100 described CPPs have been compared systematically making the choice of “the” CPP for a given application difficult. The goal of the presented work is the CPP mediated delivery of the pro-apoptotic peptide KLA in breast cancer cells as proof of principle for a therapeutical application. Analysed were (I) Different CPPs in various cell types to select the “best” one, (II) The influence of the CPP C-termini on uptake and localisation, (III) The cellular KLA delivery via a non-covalent CPP administration. 22 CPPs were compared in seven cell types thereby looking at toxicity, cellular uptake and subcellular localisation using fluorescein labelled CPPs for fluorescence spectroscopy and confocal microscopy. The resulting uptake information allowed the classification of the CPPs in three main groups. The evaluation of carboxylated and carboxyamidated CPP C-termini revealed that a carboxyamide mostly enhanced the cellular CPP uptake. Three CPPs were selected (MPG, penetratin and integrin) to deliver the pro-apoptotic KLA peptide in two cancer cell lines (breast cancer MCF-7 cells and RAW264.7 macrophages) compared to fibroblasts (Cos-7) via the non-covalent strategy. A successful KLA delivery depended on the applied CPP, its C-terminus and the used cell line. The biological activity of the pro-apoptotic KLA peptide was determined via the cell viability (MTT assay). The co-incubation of MPG-CONH2:KLA (1:2) was able to induce toxicity in breast cancer cells and leukaemic macrophages, but not in fibroblasts. The viability reductions were then assigned to apoptosis. This work provides important information for the choice of an adequate CPP for the pro-apoptotic KLA peptide delivery and presents the advantage of the non-covalent delivery strategy.