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The non-linear stress-strain behavior of the human periodontal ligament and its effect on finite element models of dental structuresDurkee, Mark Carlton, January 1996 (has links)
Thesis (Ph. D.)--University of Maryland, 1996. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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The non-linear stress-strain behavior of the human periodontal ligament and its effect on finite element models of dental structuresDurkee, Mark Carlton, January 1996 (has links)
Thesis (Ph. D.)--University of Maryland, 1996. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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A study of periodontal ligament mesial to the mouse mandibular first molar /Freezer, Simon Richard. January 1984 (has links) (PDF)
Thesis (M.D.S.)--University of Adelaide, 1985. / Includes bibliographical references (leaves 184-220).
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Timecourse, dynamics, stability, and molecular determinants of fibroblast-traction-mediated collagen patterning /Sawhney, Ravi Kumar. January 2001 (has links)
Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 96-101).
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New attachment following the surgical treatment of periodontitis in dogs a thesis submitted in partial fulfillment ... of periodontics ... /Card, Steven J. January 1986 (has links)
Thesis (M.S.)--University of Michigan, 1986.
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Innervation of the human periodontal membrane and gingiva thesis submitted in partial fulfillment ... orthodontics /Kirstine, William D. January 1957 (has links)
Thesis (M.S.)--University of Michigan, 1957.
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New attachment following the surgical treatment of periodontitis in dogs a thesis submitted in partial fulfillment ... of periodontics ... /Card, Steven J. January 1986 (has links)
Thesis (M.S.)--University of Michigan, 1986.
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A study of the terminal nerve endings in the periodontal membrane and gingiva thesis submitted as partial fulfillment ... /Gach, Lewis. January 1956 (has links)
Thesis (M.S.)--University of Michigan, 1956.
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The effect of intermittent tensile strain on RANKL, OPG, M-CSF and IL-1β expression by periodontal ligament fibroblasts in vitroGaffey, Benjamin James, n/a January 2007 (has links)
Mechanical stress has been shown to play a role in bone remodelling during orthodontic tooth movement. Receptor activator of nuclear factor kβ - ligand (RANKL), osteoprotegerin (OPG), monocyte colony stimulating factor (M-CSF) and interleukin 1-β (IL-1β) play key roles in the regulation of bone remodelling, but the role of these cytokines in orthodontic tooth movement is poorly understood.
Aim: The aim of this experiment was to examine the response of periodontal ligament (PDL) fibroblasts in monolayer culture to intermittent tensile stress as regards RANKL, OPG, M-CSF and IL-1β production.
Methods: Human PDL fibroblasts were dissected from premolars extracted for orthodontic purposes. Explants were seeded out in 1cm wells and grown to confluence in Dulbecco�s modification of Eagle�s medium, containing 10% foetal calf serum and antibiotics, at 37�C in a humidified atmosphere of 5% CO₂/95% air. Upon reaching confluence, the cells were passaged into sequentially larger flasks. Fibroblasts were passaged 6 times. After reaching confluence in T175 flasks, the cells were detached and plated at a cell density of 10⁵/dish in 35mm Bioflex� Plates coated with type 1 collagen. The cells were placed under a continuous uni-axial strain of 12% for 6s of every 90s by a Flexercell FX 4000C[TM] for 0, 12, 24 and 48 hours. Cells were then detached and stored in RNAlater. Quantitative RT-PCR was used to determine the mRNA of the cytokines of interest.
Results: Tensile force led to the down regulation of mRNA expression for OPG and IL-1β at 12 and 24 hours respectively, while M-CSF was up-regulated at 6 hours. RANKL was not detected at a significant level for quantification.
Conclusion: This osteoclastic-type response indicates the complexity of mechanotransduction in an in vitro setting.
Acknowledgments: This research was supported by the New Zealand Dental Research Foundation, the New Zealand Lottery Grants Board and the New Zealand Association of Orthodontists.
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The effect of intermittent tensile strain on RANKL, OPG, M-CSF and IL-1β expression by periodontal ligament fibroblasts in vitroGaffey, Benjamin James, n/a January 2007 (has links)
Mechanical stress has been shown to play a role in bone remodelling during orthodontic tooth movement. Receptor activator of nuclear factor kβ - ligand (RANKL), osteoprotegerin (OPG), monocyte colony stimulating factor (M-CSF) and interleukin 1-β (IL-1β) play key roles in the regulation of bone remodelling, but the role of these cytokines in orthodontic tooth movement is poorly understood.
Aim: The aim of this experiment was to examine the response of periodontal ligament (PDL) fibroblasts in monolayer culture to intermittent tensile stress as regards RANKL, OPG, M-CSF and IL-1β production.
Methods: Human PDL fibroblasts were dissected from premolars extracted for orthodontic purposes. Explants were seeded out in 1cm wells and grown to confluence in Dulbecco�s modification of Eagle�s medium, containing 10% foetal calf serum and antibiotics, at 37�C in a humidified atmosphere of 5% CO₂/95% air. Upon reaching confluence, the cells were passaged into sequentially larger flasks. Fibroblasts were passaged 6 times. After reaching confluence in T175 flasks, the cells were detached and plated at a cell density of 10⁵/dish in 35mm Bioflex� Plates coated with type 1 collagen. The cells were placed under a continuous uni-axial strain of 12% for 6s of every 90s by a Flexercell FX 4000C[TM] for 0, 12, 24 and 48 hours. Cells were then detached and stored in RNAlater. Quantitative RT-PCR was used to determine the mRNA of the cytokines of interest.
Results: Tensile force led to the down regulation of mRNA expression for OPG and IL-1β at 12 and 24 hours respectively, while M-CSF was up-regulated at 6 hours. RANKL was not detected at a significant level for quantification.
Conclusion: This osteoclastic-type response indicates the complexity of mechanotransduction in an in vitro setting.
Acknowledgments: This research was supported by the New Zealand Dental Research Foundation, the New Zealand Lottery Grants Board and the New Zealand Association of Orthodontists.
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