Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1959. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 81-85).

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No. of bitstreams: 1 silva_jt_me_jabo.pdf.pdf: 1474757 bytes, checksum: 4d1f43374ae32ef5697a09a929e48a61 (MD5) Previous issue date: 2017-10-20 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Para um bom desenvolvimento e produtividade a cultura de eucalipto exige, principalmente nos primeiros estágios do seu desenvolvimento, grande quantidades de fósforo (P), que é um nutriente essencial para o metabolismo do eucalipto, sua ausência afeta o desenvolvimento comprometendo sua produtividade. Devido à baixa disponibilidade desse nutriente nos solos brasileiros, altas doses de adubos fosfatados são utilizadas a fim de suprir a necessidade nutricional da planta, o que não obtém sucesso, devido à precipitação e combinação com as partículas do solo. O objetivo do presente estudo foi avaliar a atividade das bactérias Azospirillum brasilense e Bacillus subtilis em solubilizar fósforo para mudas de eucalipto no período inicial do seu desenvolvimento. As mudas foram replantadas em vasos e receberam os inóculos em três doses, sendo 1ml, 10ml e 20ml na concentração 1x10⁷. Após o plantio as mudas foram aspergidas diariamente com água e permaneceram em condições ambientais, sendo avaliadas nos períodos de 40 e 70 dias após o plantio. Comparando os parâmetros de crescimento das mudas de eucalipto e os períodos de avaliação, não houve diferenças significativas entre os tratamentos. O número de bactérias totais variou entre tratamentos e períodos, mas não se verificou aumento significativo quando comparados ao controle. As concentrações de fósforo solúvel no solo, não apresentaram diferenças significativas entre si, os tratamentos que receberam os inóculos bacterianos apresentaram concentrações de fósforo no solo semelhantes quando comparados ao controle. As quantidades de fósforo nas folhas variaram entre os tratamentos e controle, e não verificou aumento nas concentrações entre os tratamentos. Os resultados sugerem que a inoculação do Azospirillum brasilense e Bacillus subtilis solubilizadores de fósforo no período inicial de desenvolvimento da cultura de eucalipto não apresentaram diferenças entre os tratamentos e as doses, embora não apresentassem uma promoção no crescimento das plantas, as concentrações de fósforo mostraram-se semelhantes ao controle. / For a good development and productivity the eucalyptus crop demands, mainly in the early stages of its development, great amounts of phosphorus (P), which is an essential nutrient for the metabolism of eucalyptus, its absence affects the development compromising its productivity. Due to the low availability of this nutrient in Brazilian soils, high doses of phosphate fertilizers are used in order to supply the nutritional need of the plant, which is not successful due to precipitation and combination with the soil particles. The objective of the present study was to evaluate the activity of the bacteria Azospirillum brasilense and Bacillus subtilis in solubilizing phosphorus for eucalyptus seedlings in the initial period of its development. The seedlings were replanted in pots and inoculated in three doses, being 1ml, 10ml and 20ml in the 1x10 concentração concentration. After planting the seedlings were sprayed daily with water and remained in environmental conditions, being evaluated in the periods of 40 and 70 days after planting. Comparing the growth parameters of eucalyptus seedlings and the evaluation periods, there were no significant differences between treatments. The number of total bacteria varied between treatments and periods, but there was no significant increase when compared to the control. The concentrations of soluble phosphorus in the soil did not present significant differences among them, the treatments that received the bacterial inocula had similar concentrations of phosphorus in the soil when compared to the control. The amounts of phosphorus in the leaves varied between the treatments and control, and did not verify increase in the concentrations between the treatments. The results suggest that the inoculation of Azospirillum brasilense and Bacillus subtilis phosphorus solubilizers in the initial period of development of the eucalyptus crop showed no differences between the treatments and the doses, although they did not present a promotion in the growth of the plants, if similar to the control.

Magister Scientiae - MSc (Biotechnology) / Endophytic bacteria are known to have an endosymbiotic relationship with plants and provide them with many beneficial properties. These bacteria stimulate plant hormones, provide protection from pathogens and increase nutrient availability in the environment. In this study some of these potential growth factors were tested. Endophytic bacteria have the potential to be of great value for the increase of crop production. They offer a variety of processes that aid in plant growth promotion in an ecofriendly manner. The use of endophytic bacteria provides a cheaper and cleaner approach compared to industrial made fertilizers. They also have potential uses in bioremediation to clean the environment polluted by industrial processes. Endophytes were isolated and showed significant growth improvement. Each isolate displayed different morphologies. Isolates were tested for classical growth promotion mechanisms such as the ability to solubilize phosphate, Indole-3-acetic acid and siderophore production. Inductively Coupled Plasma Optical Emission Spectrometry was performed to measure the effect of the isolates on the plants nutrient profile. The isolates were then tested again while the plants were under heavy metal stress to determine if they were still capable of growth promotion. The plants were then assayed for cell death using Evans blue and biomass was measured to determine the effect of vanadium stress. Inductively Coupled Plasma Optical Emission Spectrometry was performed again to assess the change in nutrient profile while under vanadium stress. / 2021-08-31

The germination rates of four plant species (Allium schoenoprasum L., Dianthus gratianopolitanus ‘Grandiflorus’ Vill., Sedum kamtschaticum Fisch. & C.A. Mey. Spp. ellacombianum (Praeger) R.T. Clausen, and Talinum calycinum Engelm.) were compared with six fertility treatments (control, fertilizer - once a week, double fertilizer - twice a week, mycorrhizae, vermicompost, and green roof mix) that also evaluated a peat based greenhouse medium verses a lightweight aggregate medium integrated. The greenhouse medium had higher germination rates than the lightweight aggregate medium. The control, fertilizer, and double fertilizer, which were not applied until a month into the study, provided similar germination results. The seedlings obtained from the germination study were grown into 3.81cm x 7.62cm plugs, with plant height, leaf count/width, and dry weight taken three months later. The greenhouse medium treatments control, fertilizer, and double fertilizer had the most leaves, and provided the tallest and widest leaves as well as the greatest biomass. The lightweight aggregate medium control displayed the lowest growth in this study. The fertility treatment that showed the most potential was the green roof mix. This mix significantly improved plant growth in the lightweight aggregate medium, which indicates the potential to improve greenhouse medium based plug establishment on extensive green roofs with aggregates.

Orientador: Elizabeth Orika Ono / Coorientador: Nobuyoshi Narita / Banca: João Domingos Rodrigues / Banca: Jose Carlos Cavichioli / Resumo: O mercado de frutas exóticas no Brasil tem ganhado prestígio e o cultivo da pitaya apresenta-se promissor. Porém, o seu ciclo é limitado ao longo do ano, principalmente, pela influência do fotoperíodo no seu florescimento. A utilização de regulador vegetal, sobretudo a giberelina (GA), pode substituir a necessidade de dias longos. Nesse sentido se estudou diferentes épocas de aplicação e várias concentrações de GA3 no período não indutivo com o objetivo de induzir e antecipar o florescimento. O delineamento experimental utilizado foi blocos ao acaso em esquema fatorial 3x5, sendo três épocas de aplicação e cinco concentrações do regulador vegetal. As épocas de aplicação iniciaram em maio, junho e julho, sendo realizadas três aplicações a cada 30 dias. As concentrações estudadas foram 0, 100, 200, 300 e 400 mg L-1 de GA3. A época de aplicação de GA3 não influenciou em nenhuma das características estudadas, por outro lado o fator concentração, apesar de não ter antecipado o florescimento, proporcionou aumento na fixação de frutos, número de frutos, massa média de frutos e produtividade. Com base nos resultados obtidos é possível concluir que a aplicação de GA3 foi benéfica às características agronômicas da pitaya / Abstract: The exotic fruit market in Brazil has gained prestige and cultivation of pitaya presents promising. But the cycle is limited throughout the year, and its flowering by photoperiod. The use of plant growth regulator, especially gibberellins, can replace the need for long days, in this sense, we studied different application periods and some concentrations of GA in non-inductive period in order to anticipate flowering. The experimental design was randomized blocks in factorial scheme 3x5, being three times of application and five concentrations of the GA3. The application periods began in May, June and July, and three times application, one every 30 days. The concentrations studied were 0, 100, 200, 300 and 400 mg L-1 GA3 application timing did not influence any of the traits on the other hand the concentration factor, despite not having anticipated the flowering could provide increased fruit set, fruit number, mean fruit mass and productivity. Based on the results, we concluded that the GA3 application was beneficial to the agronomic characteristics of pitaya / Mestre

Orientador : Elizabeth Orika Ono / Banca: João Domingos Rodrigues / Banca: Vandeir Francisco Guimarães / Banca: Manoel Abilio de Queiroz / Banca: Silvio José Bicudo / Resumo: Tratamentos com reguladores vegetais foram estudados em plantas de soja (Glycine max (L.) Merrill cv. BRS-184) para avaliar o teor de clorofila das folhas, a altura da planta, o número de ramificações e altura de vagens. O experimento foi conduzido em casa de vegetação do Departamento de Botânica do Instituto de Biociências de Botucatu - UNESP. O delineamento experimental utilizado foi inteiramente casualizado, com três repetições e oito tratamentos (testemunha; GA3 100 mg L-1; BAP 100 mg L-1; IBA 100 mg L-1; Stimulate® (IBA+GA3+Cinetina) 20 mL L-1; cloreto de mepiquat 100 mg L-1; cloreto de mepiquat 100 mg L-1+BAP 100 mg L-1+IBA 100 mg L-1 e ethephon 600 mg L-1. Os tratamentos foram aplicados três vezes, espaçados em 30 dias durante o ciclo da planta a partir do 43º dia e foram realizadas seis avaliações espaçados em 13 dias. Os tratamentos com BAP, ethephon e cloreto de mepiquat + IBA + BAP mantiveram o teor de clorofila nas folhas alto até o final do ciclo da planta. A altura de plantas foi menor que a testemunha em todos os tratamentos a partir de 90 dias após a semeadura, com exceção do tratamento com GA3, que manteve a altura de plantas superior durante todo ciclo da cultura. As ramificações das plantas foram incrementadas pelo tratamento com ethephon, que apresentou, a altura da primeira vagem inferior à testemunha. No tratamento com GA3, a altura da primeira vagem foi superior. / Abstract: Treatments with plant growth regulators were studied in soybean plants (Glycine max (L.) Merrill cv. BRS-184) to assess the leaf chlorophyll content, the ramification number and the height of the pods. The experiment has been carried out at the greenhouse of the Botanic Department, Bioscience Institute, University of the Sao Paulo State - UNESP, campus of Rubiao Junior, Botucatu, Brazil. The used experimental design delineation was completely randomized block with three replications and eight treatments (control; GA3 100 mg L-1; BAP 100 mg L-1; IBA 100 mg L-1; Stimulate® (IBA+GA3+Cinetin) 20 mL L-1; mepiquat chloride 100 mg L-1; mepiquat chloride 100 mg L-1+BAP 100 mg L-1+IBA 100 mg L-1 and ethephon 600 mg L-1. The treatments were applied three times every 30 days during the plant cycle after 43 days and six evaluations have been made every 13 days. The treatments with BAP, ethephon and mepiquat chloride + IBA + BAP maintained the high chlorophyll content until the end of the plant cycle. The height of the plants was lower than the control in all treatments after the 90th day of sowing, excepting the treatment with GA3, which maintained the height of the plants during the cycle of cultivation. The plant stems were increased by the ethephon treatment; however, the height of the first pod was the lowest in this treatment and the highest in the treatment with GA3. / Doutor

Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-05-04T13:51:09Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) dissertaçãoSILVA2016.pdf: 1538144 bytes, checksum: 5e9ed18ffcbde4ab9a92d5f040477ab7 (MD5) / Made available in DSpace on 2017-05-04T13:51:09Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) dissertaçãoSILVA2016.pdf: 1538144 bytes, checksum: 5e9ed18ffcbde4ab9a92d5f040477ab7 (MD5) Previous issue date: 2016-02-21 / FACEPE / O paclobutrazol (PBZ) é o regulador vegetal utilizado no manejo da produção da mangueira na maioria dos pomares sob as condições semiáridas do Nordeste. Apesar da sua eficiência na produtividade, estudos demonstram que o PBZ permanece ativo no solo por muito tempo e isso pode afetar o desenvolvimento das próximas colheitas, reduzindo o vigor vegetativo. A biodegradação do paclobutrazolem solo não saturado e com histórico de aplicação,foi avaliada durante 49 dias usando glicerol e resíduos industriais (glicerol do biodiesel, torta de amendoim e torta de gergelim) como fontes de adicionais de carbono. Os resíduos agroindustriais (torta de amendoime e torta de gergelim) além de carbono, também são fontes de nitrogênio. Foram realizados estudos cinéticos do consumo de PBZ, além da contagem dos micro-organismos totais, do perfil do pH e da fitotoxicidade no início e final dos experimentos. Adicionalmente, estudos investigativos acerca de produtos da biodegradação foram realizados, com análises de espectroscopia de infravermelho. A torta de amendoim foi o melhor resíduo agroindustrial, sendo usado nas quatro estratégias realizadas na próxima etapa. Nessas quatro estratégias de biodegradação foram realizados experimentos usando 100, 80, 50 e 20 % m/m do solo sem histórico com a adicão de 0, 20, 50 e 80 % m/m de solo com histórico, respectivamente. A adição de solo com histórico (50 ou 80% m/m) e a torta de amendoim favoreceram a biodegradação dosolo sem histórico, cuja biodegradação foi maior que 95%. O resíduoselecionado apresentou-se como uma adequada fonte de carbono e de nitrogênio, e, o solo com histórico um eficiente reservatório de micro-organismos capazes de degradar o PBZ. / Paclobutrazol (PBZ) is a growth regulator used in the management of mango production in most orchards in semi-arid conditions of the Northeast. Despite its efficiency in productivity, studies show that the PBZ remains active in the soil for a long time and this may affect the development of the next crop, reducing plant vigor. The biodegradation of paclobutrazol (PBZ) was evaluated during 49 days using glycerol and agro-industrial wastes (biodieselderived glycerol, peanut cake and sesame cake) as additional carbon sources and unsaturated soil with a history of PBZ application. Kinetic studies were performed of PBZ consumption beyond count of total microorganisms, pH profile and phytotoxicity at the beginning and end of the experiments. Additionally, investigative studies on the caracterization of biodegradation samples were performed with infrared spectroscopy analysis. Biodieselderived peanut cake was the best agro-industrial waste, andthis was used in the following four strategies. Four biodegradation strategies were performed using 100 %, 80 %, 50 % and 20 % of soil without history with the addition of 0 %, 20 %, 50 % and 80 % of soil with history, respectively. The addition of soil with history (50 or 80%) and the selected waste (peanut cake) favored biodegradation of soil without history, with rates about 95 %. The peanut cake waste proved to be an adequate source of carbon and nitrogen, and the soil with history, proved to be an efficient reservoir of microorganisms capable of biodegrading PBZ.

Plant growth and survival are affected by the nutrients available in the environment. Nitrogen (N) is most often the limiting nutrient in terrestrial ecosystems, particularly in temperate and boreal forests, such as those on Vancouver Island. To overcome the challenge of limited nutrient availability, plants have evolved symbiotic relationships with fungi, called mycorrhizae. While research on the importance of mycorrhizal symbioses for N uptake by plants continues to grow, we have a limited understanding of the mechanisms of N uptake and transfer by mycorrhizae. This knowledge is crucial to fully understand N uptake and assimilation by plants. This study aimed to determine the influence of soil N availability on conifer growth and foliar N content, and on the N form preferences and sporocarp N content of associated mycorrhizae. Inorganic and organic soil N production was determined for two sites, Fairy Lake and San Juan, near Port Renfrew British Columbia, under pure plantations of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco), Sitka spruce (Picea sitchensis [Bong.] Carr.), western redcedar (Thuja plicata Donn ex D. Don in Lamb) and western hemlock (Tsuga heterophylla [Raf.] Sarg.). Ammonium, nitrate and amino acid production contrasted between the sites, with relatively higher N production in San Juan compared to Fairy Lake. This indicated differences in soil N cycling, most likely due to differences in moisture and topography. In general, conifer species did not affect inorganic and organic soil N production. Growth of conifers increased with increasing N availability, and differed between species, with Douglas-fir and Sitka spruce having the greatest growth and western redcedar having the least growth. Foliar %N and 15N were found to differ among the conifer species, and western redcedar had the lowest foliar N concentrations. While site quality was not reflected in foliar %N, foliar 15N was found to increase with increasing 15N of the forest floor. Ectomycorrhizal (ECM) sporocarps reflected site quality, with greater N concentrations but lower 15N values on the higher N site. Sporocarp 15N concentrations were higher than foliar 15N concentrations, suggesting N isotope fractionation by mycorrhizae. Finally, site N availability was not related to the rates of N form uptake by ECM genera. Both ECM and arbuscular mycorrhizae (AM) did not have substantial nitrate uptake, despite a greater supply of nitrate. Ammonium was found to be taken up at higher rates than nitrate in the ECM and AM roots, suggesting a preference for ammonium, possibly due to ammonium being energetically cheaper to metabolize and suppressing nitrate transporters in mycorrhizal fungi. Differences in proportions of N form uptake and sporocarp N content among ECM genera were seen, indicating potential niche formation based on functional traits such as N form uptake and mycelial morphology. Knowing how mycorrhizae respond to different N forms and rates of N supply will not only increase our knowledge of N dynamics in mycorrhizal symbioses, but will help predict the effects environmental changes, such as disturbance and N deposition, may have on these systems. / Graduate / 2018-08-09