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Quantitation of cyanide detoxification product ß-cyanoalanine by LC-MS/MS in plant tissue and mitochondrial preparationWu, Jianfeng, 吳劍鋒 January 2014 (has links)
abstract / Biological Sciences / Master / Master of Philosophy
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Cloning and expression of adropin a novel secreted peptide related to obesity and its related disorders /Wen, Yongna, Wendy. January 2009 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2009. / Includes bibliographical references (p. 54-61).
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Biosynthesis and translocation of secondary metabolite glycosides in the grapevine Vitis vinifera L. /Gholami, Mansour. January 1996 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 1996? / Copies of author's previously published articles inserted. Includes bibliographical references (leaves 121-144).
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Cloning and expression of adropin: a novel secreted peptide related to obesity and its related disordersWen, Yongna, Wendy., 溫詠娜. January 2009 (has links)
published_or_final_version / Pharmacology and Pharmacy / Master / Master of Medical Sciences
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Some effects of minor nutrients on the growth and metabolism of plantsPossingham, John V. January 1956 (has links)
Investigations are described which were carried out to analyse the way in which certain mineral element deficiencies restrict the growth and development of plants. The plant system used in this work was excised pea roots grown in sterile culture media, and the deficiencies studied were those of iron, magnesium and molybdenum. Growth was measured at the cell level and related to other characteristics of the system; two different experimental designs being employed to assess the effects of deficiencies. In the first, roots were grown in full nutrient and in deficient media and growth was measured on samples taken after growing periods of 0, 3, 5, 7, 9 and 11 days; while in the second, roots were grown for 7 days in full nutrient and in deficient media and serial one centimetre sections taken from these roots were compared. The first approach assessed the effects of the deficiency on overall growth, and the second gave an indication of the effects of the deficiency on the longitudinal differentiation of pea roots. Both experimental approaches were employed when examining iron and magnesium deficient roots, but only the second when examining molybdenum deficient roots. Roots deficient in iron and magnesium were obtained by culturing tips cut from germinated seeds in deficient media, but two successive tip passages were necessary to obtain roots deficient in molybdenum. Growth was assessed basically in terms of length, cell volume, protein nitrogen, and rate of oxygen uptake. However with iron deficient roots measurements of invertase activity, sensitivity of the oxygen uptake to cyanide, and the frequencies of cells in the different stages of division were also made. The techniques involved in the culture of deficient and full nutrient roots, and the analytical techniques are described. It has been shown that iron deficiency markedly affects the growth and development of excised pea roots. Growth in terms of length and cell number per root is stopped after 7 days and no further increases occur between days 7 and 11. Although iron deficiency stops cell division, measurements made at day 7 indicate that this deficiency does not restrict the process of cell expansion. In fact 7 day old iron deficient roots carry larger cells in the terminal centimetre than full nutrient roots. By 11 days the iron deficient roots have a pronounced swelling at the terminal end, and it is suggested that this is brought about by an abnormal expansion of the cells in the lateral direction. Some cells containing mitotic figures are present in the tips of 7 and 11 day old iron deficient roots. However there are fewer cells in the division stages of prophase and metaphase and practically no cells in the stages of telophase and anaphase in the deficient roots when comparisons were made with full nutrient roots. The protein nitrogen content of iron deficient roots is lower than that of full nutrient roots at day 7, but there is a considerable increase in both deficient and full nutrient roots between days 7 and 11. The trend of the derived quantity, average protein nitrogen content per cell, is the same in both groups of roots up to day 7, but from day 7 to day 11 it increases sharply in the deficient roots but does not change in the full nutrient roots. This result indicates that cell division was not stopped in the deficient roots by a shortage of protein nitrogen as such. At the day 7 stage the distribution of protein nitrogen along the length of deficient roots is different to that in full nutrient roots. The front sections of the deficient roots contain an increased content and the back sections a decreased content when compared with full nutrient roots. On a per cell basis the situation is the same, as the cells in the front sections of deficient roots have a higher average protein content and those in the back sections a lower content when compared with the cells of full nutrient roots. The accumulation of protein nitrogen in the front sections of iron deficient roots is most probably associated with the cessation of active cell division in the meristem. Evidence is available which suggests that under normal conditions the formation and development of cells in the apex of the root is dependent on substrates synthesised in the mature regions of the root and translocated forward. It is considered that in iron deficient roots precursors of protein are no longer removed by the demands of the meristem and they condense to form protein in the regions adjacent to the apex. Invertase activity per unit protein nitrogen is the same in both full nutrient and iron deficient roots at all stages. Further, there is no difference in invertase activity when the corresponding sections of full nutrient and deficient roots are compared at day 7. It is clear that in this one respect the protein of iron deficient roots is similar to that of full nutrient roots. The rate of oxygen intake per root of iron deficient roots is lower than that of full nutrient roots at the early day 3 stage, but there are large increases in the rates in both deficient and full nutrient roots between days 3 and 11. It is of some significance that iron deficiency clearly reduces the rate of oxygen uptake at a stage before the process of cell division is stopped. On a per unit protein nitrogen basis the rate of oxygen uptake of deficient roots is lower than that of full nutrient roots after day 3. It is suggested that the effects of days 3 and 5 are a direct effect of iron deficiency but the effects at days 9 and 11 are influenced by the fact that cell division stops at day 7. The results from 7 day roots show that the effect of iron deficiency in reducing the rate of oxygen intake per unit protein nitrogen is confined to the front three sections of the root as iron deficiency does not alter the rates in the back three sections. Iron recovery experiments show that iron deficient roots 7, 9 and 11 days old can resume cell division and grow when they are transferred to a full nutrient medium. It is of interest that in these experiments the recovery in terms of an increased rate of oxygen uptake is greater than the recovery in terms of length and protein nitrogen. Experiments in which the rate of oxygen uptake of deficient and full nutrient roots were measured in the presence and absence of cyanide show that in both groups of roots there is a large fraction of the respiration insensitive to cyanide. The activity of this cyanide insensitive system increases considerably from day 3 to day 11 in both the full nutrient and iron deficient roots. Increases, after day 3 in the activity of this cyanide insensitive system, which would not contain iron, account for the large increase in the total rate of oxygen uptake of iron deficient roots between days 3 and 11. The activity of the cyanide sensitive system involved in respiration decreases in both groups of roots between days 0 and 5. It increases from day 5 to 11 in full nutrient roots, but does not increase in deficient roots over this period. That synthesis of a cyanide sensitive system involved in respiration stops at or about the same stage as cell division in iron deficient roots is considered to be highly important. This cyanide sensitive system most probably corresponds to the iron containing cytochrome/cytochrome oxidase system, and there is other circumstantial evidence that this system is important in the process of cell division. It is important to note that the activity of the cyanide sensitive system was the same in the tips of deficient and full nutrient roots at the day 7 stage. It may be that a certain minimum level of activity per cell is necessary to maintain division; a slight reduction stopping cell division completely, but not being capable of detection by the method of measurement.
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Some factors affecting uptake and utilization of ions by stem segmentsPalmer, J. M. January 1962 (has links)
No description available.
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Synthetic approaches to quinolizidine alkaloids.Jungmann, Christa Maria January 1992 (has links)
A Dissertation submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the Degree of
Master of Science. / An outline of reported synthetic routes to the Lupine
alkaloids, epilamprolobine [2] and lamprolobine [3] and a
review of the use of vinylogous amides and urethanes as
precursors for the synthesis of alkaloids are presented in
Chapter 1. This is followed by a presentation of our
strategy for synthesis of the two Lupine alkaloids.
Vinylogous cyanamide intermediate 1- (3-hydroxypropyl) -2-
cyanomethylenepiperidine [68] plays a key role in this
strategy, since exploitation of its ambident nucleophilicity forms the central theme of this project,
The successful route to the intermediate [68] involved the
preliminary preparation of the tertiary thiolactam, 1-(2-
ethoxycarbonylethyl)piperidine-2-thione [83][ by thiation
of the secondary lactam 2-piperidinone [72] and conjugate
addition at nitrogen with ethyl acrylate in a Michael
reaction. Sulphur extrusion of the salt made from [83] and
bromoacetonitrile and subsequent reduction of the ester
group provided the pivotal vinylogous cyanamide intermediate.
A number; of alternative routes based on 5-
bromopentanoic acid [80], 1-allyl-2-piperidinone [73] and
thiolactams [84J and [105] were unsuccessful.
Cyclisation of the intermediate [68] was achieved by an
intramolecular c-alkylative ring closure via the
corresponding tosylate [l16] to forln an unsaturated
functionalised quinolizidine [69]. Stereoselective carboncarbon
double bond reduction and nitrile reduction resulted
in the synthesis of two quinolizidines. lupinamine [11] and
epilupinamine [112]. Further transformations led to the
formation of the derivatives, N-acetyllupinamine [113] and
N-acetylepilupinamine [114], and also to the target
alkaloids, epilamprolcbine [2] and lamprolobine [3]. / Andrew Chakane 2018
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Biosynthesis and translocation of secondary metabolite glycosides in the grapevine Vitis vinifera L.Gholami, Mansour. January 1996 (has links) (PDF)
Copies of author's previously published articles inserted. Bibliography: leaves 121-144. This study investigates the site of biosynthesis of flavour compounds in the grapevine. Most of the secondary metabolites, including flavour compounds, are glycosylated and stored in plant tissues as glycosides. The chemical properties of these compounds, especially their water solubility, suggests that glycosides might be forms of translocated secondary metabolites in plants.
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Biosynthesis and translocation of secondary metabolite glycosides in the grapevine Vitis vinifera L. / by Mansour Gholami.Gholami, Mansour January 1996 (has links)
Copies of author's previously published articles inserted. / Bibliography: leaves 121-144. / xiii, 150 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This study investigates the site of biosynthesis of flavour compounds in the grapevine. Most of the secondary metabolites, including flavour compounds, are glycosylated and stored in plant tissues as glycosides. The chemical properties of these compounds, especially their water solubility, suggests that glycosides might be forms of translocated secondary metabolites in plants. / Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 1996?
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Studies on thiarubrine, a naturally occurring disulfide polyineConstabel, Carsten Peter January 1988 (has links)
Chemical and biological aspects of thiarubrine, a highly antifungal dithiacyclohexadiene polyine, were investigated. A tissue culture system for the production of thiarubrines was developed by culturing hairy roots of Chaenactis douglasii induced by Agrobacterium rhizogenes strain TR7. One culture line accumulated two times the levels of thiarubrines of nontransformed control root cultures, while maintaining rapid growth. The combination of fast growth and high thiarubrine accumulation could not be duplicated in controls by adding exogenous NAA to the culture medium. Hairy root cultures also produced less thiarubrine B relative to thiarubrine A compared to controls. Thiarubrine synthesis appears to be closely correlated with degree of tissue differentiation; it is suggested that it may be more practical to improve the growth rate of thiarubrine-producing root cultures by transformation rather than seek to induce synthesis in fast-growing suspension cultures.
The biosynthetic relation between thiarubrines and the always co-occurring thiophenes was investigated by performing ³⁵S tracer experiments with C. douglasii hairy root cultures. It is possible that the thiophenes are not actively synthesized by the roots but rather are products of thiarubrine decomposition resulting from the extraction procedures and other manipulations of the cultures. The in vitro conversion of thiarubrine to thiophene can be induced by light, heat and other agents. No turnover of thiarubrines could be detected in the cultures in late logarithmic or stationary phases of the growth cycle. I
Thiarubrines show strong light-independent antibacterial and antifungal activity. The mechanism of action of thiarubrine against E. coli and S. cerevisiae was investigated using comparative disk bioassays. A very similiar polyine from Rudbeckia hirta was as active as thiarubrine in the dark, indicating the central role of the disulfide ring in toxicity of the compounds. Visible light enhanced this activity suggesting that decomposition of the disulfide ring is important for its antibiotic effects. The photodegradation product, a thiophene, is phototoxic, probably via both type I and type II photosensitization mechanisms.
The root culture extracts of Rudbeckia hirta yielded a new isomer of a known dithiacyclohexadiene polyine. MS and NMR analyses confirmed the cis configuration of this isomer. / Science, Faculty of / Botany, Department of / Graduate
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