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Harnessing a novel compact CRISPR-Cas13b for SARS-CoV-2 diagnosticsWang, Qiaochu 04 1900 (has links)
The outbreak of infectious diseases across the world results in huge disasters for public
health. Rapid and effective diagnostic methods are crucial for disease identification and
transmission control. Since first identified in late 2019, the pandemic of COVID-19
caused by the SARS-CoV-2 virus resulted in unprecedented catastrophe globally. To
control the further spread of COVID-19, there is an urgent need for rapid, accurate,
cost-effective, and efficient diagnostics. Recently, many CRISPR-based diagnostics
have been developed by coupling isothermal amplification methods with Cas proteinmediated
nucleic acid detection. Compared with conventional methods like RT-qPCR,
CRISPR-based assays are more cost-effective and efficient without sacrificing
sensitivity and specificity. Here, I developed a Cas13-based assay for SARS-CoV-2
detection with a novel compact Cas13b protein. In this assay, the Cas13 detection is
combined with RT-LAMP, achieving the detection of viral RNA as low as 4 copies/μl.
By utilizing a simple LED-based visualizer (P51™) instead of a plate reader, the
detection result can be visualized directly without using sophisticated instruments. The
compact Cas13b-mediated viral detection together with P51™-based visualization
enable rapid, sensitive, and portable diagnostics for SARS-CoV-2, showing great
potential in application to point-of-care testing.
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A Whole Blood/Plasma Separation Lab Chip using Hetero-packed Beads and Membrane Filters for Point-of-Care Test (POCT)Shi, Shaojie 05 October 2021 (has links)
No description available.
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Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like LesionsFrimpong, Michael, Simpson, Shirley Victoria, Ahor, Hubert Senanu, Agbanyo, Abigail, Gyabaah, Solomon, Agbavor, Bernadette, Amanor, Ivy Brago, Addo, Kennedy Kwasi, Böhlken-Fascher, Susanne, Kissenkötter, Jonas, Abd El Wahed, Ahmed, Phillips, Richard Odame 21 April 2023 (has links)
Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effective diagnosis is pivotal in the successful implementation of this control program. Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification technique offers a wider range of differentiation of pathogens including those isolated from chronic skin ulcers with similar characteristics such as Haemophilus ducreyi (H. ducreyi). We have developed a RPA assay for the simultaneous detection of Treponema pallidum (T. pallidum) and H. ducreyi (TPHD-RPA). The assay demonstrated no cross-reaction with other pathogens and enable detection of T. pallidum and H. ducreyi within 15 min at 42 °C. The RPA assay was validated with 49 clinical samples from individuals confirmed to have yaws by serological tests. Comparing the developed assay with commercial multiplex real-time PCR, the assay demonstrated 94% and 95% sensitivity for T. pallidum and H. ducreyi, respectively and 100% specificity. This simple novel TPHD-RPA assay enables the rapid detection of both T. pallidum and H. ducreyi in yaws-like lesions. This test could support the yaws eradication efforts by ensuring reliable diagnosis, to enable monitoring of program success and planning of follow-up interventions at the community level.
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