Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T14:01:53Z No. of bitstreams: 5 13.pdf: 1084974 bytes, checksum: 385f250239d33b253937c1a0da7c2522 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T11:17:33Z (GMT) No. of bitstreams: 5 13.pdf: 1084974 bytes, checksum: 385f250239d33b253937c1a0da7c2522 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T11:17:33Z (GMT). No. of bitstreams: 5 13.pdf: 1084974 bytes, checksum: 385f250239d33b253937c1a0da7c2522 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2012 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Este trabalho teve por objetivo avaliar a germina??o in vitro de sucupira-preta (Bowdichia virgilioides) e adaptar um procedimento b?sico de micropropaga??o para essa mesma esp?cie. Para a germina??o in vitro, sementes escarificadas e n?o escarificadas foram inoculadas em diferentes concentra??es e formula??es de meios de cultura suplementados com aditivos (PVP e carv?o ativado). Posteriormente, as plantas foram transferidas para tubetes e aclimatadas em casa de vegeta??o. Para a micropropaga??o, explantes foram inoculados em meio de cultura WPM, suplementado com concentra??es de BAP, constituindo a fase de multiplica??o. No alongamento, os tratamentos foram constitu?dos de combina??es de ANA e BAP adicionadas ao meio de cultura. Para a fase o enraizamento, brota??es foram inoculadas em meio contendo concentra??es de AIB ou combina??es dos aditivos (PVP e carv?o ativado) com concentra??es de ANA. Na aclimata??o, as plantas foram transplantadas para substrato e cobertas com saco de polietileno que foram, posteriormente, retirados, perfurados ou n?o-retirados, e mantidas em ambiente de laborat?rio, constituindo os tratamentos da pr?-aclimata??o. A aclimata??o em casa de vegeta??o foi realizada ap?s o per?odo de pr?-aclimata??o. A germina??o in vitro de sementes escarificadas de sucupira-preta foi alcan?ada utilizando-se os meios de cultura MS e WPM a 50% e ocorreu independentemente do tipo de aditivo utilizado. A aclimata??o de plantas germinadas in vitro ocorreu independentemente do hist?rico de aditivos ou meios de cultura utilizados. Durante a micropropaga??o da esp?cie, a multiplica??o foi alcan?ada utilizando-se segmentos cotiledonares e a concentra??o de 0,3 mg.L-1 de BAP adicionada ao meio. A combina??o de 0,3 mg.L-1 de ANA com 0,03 mg.L-1 de BAP promoveu o alongamento. A indu??o de ra?zes ocorre na aus?ncia de AIB ou em resposta ?s concentra??es de 0,5 e 2,5 mg.L-1. O carv?o ativado adicionado ao meio de cultura de enraizamento melhorou a qualidade das brota??es. A aclimata??o foi alcan?ada utilizando-se uma cobertura pl?stica entorno da planta. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT This study aimed to evaluate the in vitro germination of sucupira-preta (Bowdichia virgilioides) and develop a basic micropropagation procedure for that species.? For in vitro germination seeds scarified or not scarified were inoculated at different concentrations and types of culture medium supplemented with two types of additives (PVP or activated charcoal). Subsequently, plants were transferred to tubes and acclimatized in the greenhouse.?For micropropagation, explants were inoculated in culture medium WPM supplemented with BAP being the multiplication phase.?For elongation, the treatments were combinations of BAP with ANA that were added to the medium of culture.?For rooting, shoots were inoculated in culture medium containing concentrations of AIB or combinations of additives (PVP or activated charcoal) with concentrations ANA.?For the period of acclimatization, the plants were transplanted and covered with polythene bags which were subsequently removed or not, and kept in closed environment, providing treatments for pre-acclimatization, and later, in a greenhouse constituting acclimatization.?The culture media MS and WPM 50% promoted in vitro germination of scarified seeds.?The germination of the species occurred regardless of the type of additive used.?The acclimatization of plants germinated in vitro occurred regardless of history or culture media additives used.?During the micropropagation, explants obtained from cotyledon segments and the concentration of 0,3 mg L-1 BAP added to culture medium promoted multiplication.?The combination of 0,3 mg.L-1 ANA with 0,03 mg.L-1 BAP promoted elongation.?The induction of roots occurred in absence of AIB or in response to concentrations of 0,5 and 2,5 mg L-1.?Charcoal activated added to the culture medium increased rooting quality of the shoots.?The plastic cover around the plant promoted acclimatization.