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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
41

Efeito do ácido linoléico conjugado TRANS-10, CIS-12 na regulação do acúmulo de lípides e expressão gênica em embriões produzidos in vitro

Batista, Ribrio Ivan Tavares Pereira 25 February 2010 (has links)
Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-09-16T11:53:05Z No. of bitstreams: 1 ribrioivantavarespereirabatista.pdf: 1023205 bytes, checksum: 9149da7ed1e431e5565e4816dc74a814 (MD5) Approved for entry into archive by Diamantino Mayra (mayra.diamantino@ufjf.edu.br) on 2016-09-26T20:21:30Z (GMT) No. of bitstreams: 1 ribrioivantavarespereirabatista.pdf: 1023205 bytes, checksum: 9149da7ed1e431e5565e4816dc74a814 (MD5) Made available in DSpace on 2016-09-26T20:21:30Z (GMT). No. of bitstreams: 1 ribrioivantavarespereirabatista.pdf: 1023205 bytes, checksum: 9149da7ed1e431e5565e4816dc74a814 (MD5) Previous issue date: 2010-02-25 FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais A suplementação do ácido linoléico conjugado trans-10, cis-10 no meio de cultivo, representa uma importante alternativa para aumento da sobrevivência dos embriões após a criopreservação. No entanto este isômero de CLA no cultivo in vitro sem antioxidante pode aumentar a taxa apoptótica. O objetivo do presente estudo foi avaliar o efeito da adição CLA trans-10, cis-12 no cultivo in vitro de embriões sem antioxidante. Zigotos bovinos (n = 1.694) foram divididos em dois tratamentos: (T1) grupo controle, zigotos cultivados no meio CR2aa suplementado com soro fetal (n = 815); (T2) zigotos cultivados no meio CR2aa suplementado com soro fetal mais 100 µM CLA trans-10, cis-12. Os embriões foram avaliados quanto a desenvolvimento, quantidade de lípides e criotolerância. Transcritos dos RNA mensageiros (RNAm) dos genes selecionados foram mensurados pelo Real Time PCR. Suplementação de CLA trans-10, cis-12 não afeta significativamente a taxa de blastocisto (31,8% e 34,1% T1 e T2, respectivamente, p = 0,20) e nível dos RNAm dos genes relacionados com stress celular, apoptose e síntese de novo de ácido graxo. Quantidade de lípides e transcritos do RNAm do gene 1-acilglicerol-3-fosfato oaciltransferase 1 enzima relacionado a síntese de triglicérides foram significativamente reduzidos nos embriões cultivados na presença de CLA trans-10, cis-12 em comparação com o grupo controle. Teve aumento significativo na taxa de re-expansão dos blastocistos cultivados na presença de CLA trans-10, cis-12, após o descongelamento (34.4 e 56.3% para T1 e T2, respectivamente p = 0,002). Essa diferença não persistiu na taxa de eclosão (14,0% e 16,5% para T1 e T2, respectivamente, P = 0,62). Esses resultados mostram que o CLA trans-10, cis-12 reduz o acúmulo de lípides nos embriões pela redução nos níveis dos transcritos do gene 1-acilglicerol-3-fosfato o-aciltransferase 1 sem afetar a qualidade do embrião. Adicionalmente, este ácido graxo aumenta a taxa de re-expansão, no entanto, não melhora a taxa de eclosão. Supplementation of conjugated linoleic acid trans-10, cis-10 in the culture medium, represents an important alternative to increasing the survival of embryos after cryopreservation. However the addition of culture media with CLA trans-10, cis-12 without antioxidant may increase the apoptotic rate. The aim of this study was to evaluate the effect of adding CLA trans-10, cis-12 in vitro culture of embryos without antioxidant. Bovine zygotes (n = 1,694) were divided into two treatments: (T1) control group, zygotes cultured in CR2aa medium supplemented with fetal calf serum (n = 815), (T2) zygotes cultured in CR2aa medium supplemented with fetal calf serum plus 100 µM CLA trans-10, cis-12. Embryos were evaluated for development, amount of lipids and cryotolerance. Transcripts of messenger RNA (mRNA) of selected genes were measured by real time PCR. Supplementation of CLA trans-10, cis-12 did not significantly affect the blastocyst rate (31.8% and 34.1% T1 and T2, respectively, p = 0,20) and the mRNA level of genes related to cell stress, apoptosis and de novo synthesis of fatty acid . Lipids and transcripts of the mRNA of the gene 1-acilglicerol-3-phosphate o-acyltransferase 1 enzyme related to the synthesis of triglycerides were significantly reduced in embryos cultured in the presence of CLA trans-10, cis-12 in comparison the control group. Had increased rate re-expansion of blastocysts cultured in the presence of CLA trans-10, cis-12, after thawing (34.4 and 56.3% for T1 and T2, respectively p = 0,002). This difference did not persist in the hatching rate (14.0 and 16.5% for T1 and T2, respectively, P = 0,62). These results show that the CLA trans-10, cis-12 reduces the accumulation of lipids in the embryos by reducing the levels of gene transcripts acilglicerol-1-3-phosphate oacyltransferase 1 without affecting the quality of the embryo. Additionally, this fatty acid increases the rate re-expansion, but does not improve the hatching rate.
42

DEVELOPMENT OF A NEW ALLELIC DISCRIMINATION REAL-TIME PCR ASSAY FOR THE DIAGNOSIS OF EQUINE HERPESVIRUS-1 AND CHARACTERIZATION OF THE VIRULENCE DETERMINANTS OF THE VIRUS

Smith, Kathryn L 1 January 2013 (has links)
Equine herpesvirus-1 (EHV-1) can cause acute upper respiratory tract disease, abortion, neonatal death and neurological disease in horses. Rapid, accurate and timely diagnosis of EHV-1 infection in horses is important to curtail the spread of this pathogen. It has been reported that the neuropathogenic phenotype of EHV-1 can result from a single non-synonymous nucleotide substitution at position 2254 (A→G2254) in open reading frame 30 (ORF30). This was the basis for the development of an allelic discrimination, real-time PCR assay to distinguish between potential neuropathogenic and non-neuropathogenic EHV-1 strains. However, PCR analysis of a panel of EHV-1 abortion isolates revealed that other point mutations within ORF30 could produce false negative results with this previously described assay. Therefore, one of the objectives of this dissertation project was to develop a more sensitive and specific allelic discrimination real-time PCR assay for the detection of EHV-1. This was achieved by redesigning the primers and probes targeting ORF30. The new assay was ten times more sensitive than the original assay, with a lower detection limit of 10 infectious virus particles. While mutations within EHV-1’s genome can hinder diagnosis, they can also impact the virulence of the virus. Objective two, therefore, was to determine if sequential cell passage of T953 would induce sufficient attenuation of the EHV-1 genome to produce a low virulence phenotype. Two separate groups of 28 BALB/c mice were inoculated with either the parental strain or passage 135 (T953 P135) of EHV-1 strain T953. The animals were observed for fourteen days, euthanized and their tissues analyzed for the presence of EHV-1. At the conclusion of the fourteen day observation period, all of the mice infected with T953 P135 survived and regained their pre-inoculation body condition. Furthermore, there were significant differences in virus titer and viral DNA concentrations between T953 P135 and the parental strain, further confirming the attenuated phenotype of the virus. Taken together, data from this study clearly demonstrates that sequential cell culture passage of the neuropathogenic T953 strain of EHV-1 results in attenuation for young adult BALB/C mice.
43

Stadienspezifische Expression und Lokalisation Kalzium-abhängiger Proteinkinasen (CDPK) von Cryptosporidium parvum in der In-vitro-Kultur

Etzold, Manja 28 September 2015 (has links) (PDF)
Die Kryptosporidiose stellt aufgrund ihres zoonotischen Charakters und der Entwicklung chronischer Durchfälle bei Immunsupprimierten ein hohes Gesundheitsrisiko für den Menschen, aber ebenso für Tiere dar. Derzeit verfügbare Therapeutika ermöglichen keine zuverlässige Bekämpfung klinischer Symptome oder eine Erregerelimination, daher ist die Erforschung neuer Therapieansätze dringend notwendig. CDPK stellen in diesem Zusammenhang interessante Zielmoleküle dar, da sie zwar in Pflanzen und Protisten einschließlich Apikomplexa, jedoch nicht in Pilzen und Säugetieren vorkommen. Trotz der Entdeckung vielversprechender neuer Wirkstoffe gegen CpCDPK1 in den letzten Jahren ist zur Lokalisation und Funktion von CDPK in C. parvum wenig bekannt.Diese Arbeit belegt die Transkription von sechs CpCDPK in vitro und beschreibt erstmals die Länge der 3’UTR von CpCDPK. Die Translation wurde durch den Nachweis spezifischen Proteins in Sporozoiten im Immunoblot sowie die Lokalisation von CpCDPK1 mit Hilfe der Immunfluoreszenz belegt. Möglicherweise wird die CpCDPK1 durch N-Myristoylierung an Membranen gebunden, an die Oberfläche von Zoiten gebracht und sezerniert. Eine Rolle des Enzyms im Invasions- und Egressmechanismus des Parasiten wird diskutiert.
44

Biodegradace 17alfa-ethinylestradiolu enzymy ligninolytických hub Biodegradation of 17alfa-ethinylestradiol by enzymes of ligninolytic fungi

Přenosilová, Lenka 2012 (has links)
This work is aimed at the study of the effect of 17α-ethinylestradiol (EE2) on the production and characteristics of ligninolytic enzymes (laccase, Mn-dependent peroxidase and lignin peroxidase) in I. lacteus, T. versicolor, P. chrysosporium and P. ostreatus cultures grown on two types of liquid media. Enzyme activity production in fungal cultures was affected by the composition of culture medium. In the case of P. chrysosporium, the addition of EE2 to the complex- medium cultures led to a MnP activity stimulation and simultaneously LiP production was partially repressed in these cultures. In the mineral MM medium, no effect of EE2 on enzyme production by P. chrysosporium was observed. In EE2 treated MM cultures of P. ostreatus lower MnP activities were found when compared to biotic controls. In the case of T. versicolor cultures, the addition of EE2 to the complex medium caused laccase and LiP stimulation in the cultures. In the MM medium, however, only laccase production was affected by EE2. I. lacteus MnP production was partially repressed by EE2 in MM medium. In contrast to that, significantly higher MnP activities were detected in complex- medium I. lacteus cultures after the treatment with EE2. Further EE2 degradation by the fungal cultures was studied. The highest degradation effeciency was...
45

BK-polyomavirová infekce u pacientů po kombinované transplantaci ledviny a pankreatu BK-polyomavirus infection in patients after simultaneous pancreas and kidney transplantation

Mindlová, Martina 2011 (has links)
Introduction. The aim of the study was to introduce a new BKV PCR protocol in our centre and to verify its accuracy as well as to assess the prevalence, risk factors of BK virus replication, course of BKV infection and therapeutic approaches in simultaneous pancreas and kidney (SPK) recipients in order to design a screening protocol. Methods. The results analysed by both Affigene® and Transplantation Virology, Basel PCR protocols were compared. Thereafter 183 SPK patients were examined to assess the prevalence of BK viremia, viruria and BKVN and to identify the risk factors of BKV replication. The cases of retransplantation after a graft loss due to BKVN were retrospectively described. Results. 100 of results were analysed according to the Affigene ® and Transplantation Virology, Basel PCR protocols with the accordance of 95%, Rho = 0,946, 95% CI: 0.920 - 0.963, P<0,0001, Bland-Altman plot analyses: bias Basel PCR protocol/Affigene® BKV trender: -0,1 (mean) *±1.96 SD: -1,6 - 1,3] for both methods. Point-prevalence was assessed in 183 patients; Viruria found in 17,3 %, viremia in 3.8% of patients. High-level viruria >107 copies/mL detected in 3,7% of patiets, high-level virémia >104 in 1,6% of patients simultaneously with high-level viruria. BKVN was found in 0,5% of patients. Diabetes duration...
46

Characterization of the LYCD-Dependent Transcriptional Response in the THP-1 Cell Culture Monocytes

Osterburg, Andrew Robert 29 September 2005 (has links)
No description available.
47

The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells

Cashdollar, Jennifer Leigh 2006 (has links)
No description available.
48

THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS

MA, CI, Miss 8 October 2007 (has links)
No description available.
49

THE DEVELOPMENT AND MOLECULAR EXPRESSION IN MAMMALIAN CELLS OF AN HA-TAGGED PLASMID ENCODING FOR THE TARGET OF RAPAMYCIN (mTOR)

Dougherty, Kevin S. 18 December 2007 (has links)
No description available.
50

Vestibular schwannoma: dissecting the pathologic process and clinical applications

Welling, Duane Bradley 5 September 2003 (has links)
No description available.

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