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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
41

DEVELOPMENT OF A NEW ALLELIC DISCRIMINATION REAL-TIME PCR ASSAY FOR THE DIAGNOSIS OF EQUINE HERPESVIRUS-1 AND CHARACTERIZATION OF THE VIRULENCE DETERMINANTS OF THE VIRUS

Smith, Kathryn L 1 January 2013 (has links)
Equine herpesvirus-1 (EHV-1) can cause acute upper respiratory tract disease, abortion, neonatal death and neurological disease in horses. Rapid, accurate and timely diagnosis of EHV-1 infection in horses is important to curtail the spread of this pathogen. It has been reported that the neuropathogenic phenotype of EHV-1 can result from a single non-synonymous nucleotide substitution at position 2254 (A→G2254) in open reading frame 30 (ORF30). This was the basis for the development of an allelic discrimination, real-time PCR assay to distinguish between potential neuropathogenic and non-neuropathogenic EHV-1 strains. However, PCR analysis of a panel of EHV-1 abortion isolates revealed that other point mutations within ORF30 could produce false negative results with this previously described assay. Therefore, one of the objectives of this dissertation project was to develop a more sensitive and specific allelic discrimination real-time PCR assay for the detection of EHV-1. This was achieved by redesigning the primers and probes targeting ORF30. The new assay was ten times more sensitive than the original assay, with a lower detection limit of 10 infectious virus particles. While mutations within EHV-1’s genome can hinder diagnosis, they can also impact the virulence of the virus. Objective two, therefore, was to determine if sequential cell passage of T953 would induce sufficient attenuation of the EHV-1 genome to produce a low virulence phenotype. Two separate groups of 28 BALB/c mice were inoculated with either the parental strain or passage 135 (T953 P135) of EHV-1 strain T953. The animals were observed for fourteen days, euthanized and their tissues analyzed for the presence of EHV-1. At the conclusion of the fourteen day observation period, all of the mice infected with T953 P135 survived and regained their pre-inoculation body condition. Furthermore, there were significant differences in virus titer and viral DNA concentrations between T953 P135 and the parental strain, further confirming the attenuated phenotype of the virus. Taken together, data from this study clearly demonstrates that sequential cell culture passage of the neuropathogenic T953 strain of EHV-1 results in attenuation for young adult BALB/C mice.
42

Stadienspezifische Expression und Lokalisation Kalzium-abhängiger Proteinkinasen (CDPK) von Cryptosporidium parvum in der In-vitro-Kultur

Etzold, Manja 28 September 2015 (has links) (PDF)
Die Kryptosporidiose stellt aufgrund ihres zoonotischen Charakters und der Entwicklung chronischer Durchfälle bei Immunsupprimierten ein hohes Gesundheitsrisiko für den Menschen, aber ebenso für Tiere dar. Derzeit verfügbare Therapeutika ermöglichen keine zuverlässige Bekämpfung klinischer Symptome oder eine Erregerelimination, daher ist die Erforschung neuer Therapieansätze dringend notwendig. CDPK stellen in diesem Zusammenhang interessante Zielmoleküle dar, da sie zwar in Pflanzen und Protisten einschließlich Apikomplexa, jedoch nicht in Pilzen und Säugetieren vorkommen. Trotz der Entdeckung vielversprechender neuer Wirkstoffe gegen CpCDPK1 in den letzten Jahren ist zur Lokalisation und Funktion von CDPK in C. parvum wenig bekannt.Diese Arbeit belegt die Transkription von sechs CpCDPK in vitro und beschreibt erstmals die Länge der 3’UTR von CpCDPK. Die Translation wurde durch den Nachweis spezifischen Proteins in Sporozoiten im Immunoblot sowie die Lokalisation von CpCDPK1 mit Hilfe der Immunfluoreszenz belegt. Möglicherweise wird die CpCDPK1 durch N-Myristoylierung an Membranen gebunden, an die Oberfläche von Zoiten gebracht und sezerniert. Eine Rolle des Enzyms im Invasions- und Egressmechanismus des Parasiten wird diskutiert.
43

BK-polyomavirová infekce u pacientů po kombinované transplantaci ledviny a pankreatu BK-polyomavirus infection in patients after simultaneous pancreas and kidney transplantation

Mindlová, Martina 2011 (has links)
Introduction. The aim of the study was to introduce a new BKV PCR protocol in our centre and to verify its accuracy as well as to assess the prevalence, risk factors of BK virus replication, course of BKV infection and therapeutic approaches in simultaneous pancreas and kidney (SPK) recipients in order to design a screening protocol. Methods. The results analysed by both Affigene® and Transplantation Virology, Basel PCR protocols were compared. Thereafter 183 SPK patients were examined to assess the prevalence of BK viremia, viruria and BKVN and to identify the risk factors of BKV replication. The cases of retransplantation after a graft loss due to BKVN were retrospectively described. Results. 100 of results were analysed according to the Affigene ® and Transplantation Virology, Basel PCR protocols with the accordance of 95%, Rho = 0,946, 95% CI: 0.920 - 0.963, P<0,0001, Bland-Altman plot analyses: bias Basel PCR protocol/Affigene® BKV trender: -0,1 (mean) *±1.96 SD: -1,6 - 1,3] for both methods. Point-prevalence was assessed in 183 patients; Viruria found in 17,3 %, viremia in 3.8% of patients. High-level viruria >107 copies/mL detected in 3,7% of patiets, high-level virémia >104 in 1,6% of patients simultaneously with high-level viruria. BKVN was found in 0,5% of patients. Diabetes duration...
44

Biodegradace 17alfa-ethinylestradiolu enzymy ligninolytických hub Biodegradation of 17alfa-ethinylestradiol by enzymes of ligninolytic fungi

Přenosilová, Lenka 2012 (has links)
This work is aimed at the study of the effect of 17α-ethinylestradiol (EE2) on the production and characteristics of ligninolytic enzymes (laccase, Mn-dependent peroxidase and lignin peroxidase) in I. lacteus, T. versicolor, P. chrysosporium and P. ostreatus cultures grown on two types of liquid media. Enzyme activity production in fungal cultures was affected by the composition of culture medium. In the case of P. chrysosporium, the addition of EE2 to the complex- medium cultures led to a MnP activity stimulation and simultaneously LiP production was partially repressed in these cultures. In the mineral MM medium, no effect of EE2 on enzyme production by P. chrysosporium was observed. In EE2 treated MM cultures of P. ostreatus lower MnP activities were found when compared to biotic controls. In the case of T. versicolor cultures, the addition of EE2 to the complex medium caused laccase and LiP stimulation in the cultures. In the MM medium, however, only laccase production was affected by EE2. I. lacteus MnP production was partially repressed by EE2 in MM medium. In contrast to that, significantly higher MnP activities were detected in complex- medium I. lacteus cultures after the treatment with EE2. Further EE2 degradation by the fungal cultures was studied. The highest degradation effeciency was...
45

Characterization of the LYCD-Dependent Transcriptional Response in the THP-1 Cell Culture Monocytes

Osterburg, Andrew Robert 29 September 2005 (has links)
No description available.
46

The effects of poliovirus and astrovirus infection on dicer mRNA regulation in Caco-2 cells

Cashdollar, Jennifer Leigh 2006 (has links)
No description available.
47

THE TRANSCRIPTION FACTOR BTB AND CNC HOMOLOG 1 IN THE REGULATION OF CELL DIFFERENTIATION AND ORGANOGENESIS

MA, CI, Miss 8 October 2007 (has links)
No description available.
48

THE DEVELOPMENT AND MOLECULAR EXPRESSION IN MAMMALIAN CELLS OF AN HA-TAGGED PLASMID ENCODING FOR THE TARGET OF RAPAMYCIN (mTOR)

Dougherty, Kevin S. 18 December 2007 (has links)
No description available.
49

Vestibular schwannoma: dissecting the pathologic process and clinical applications

Welling, Duane Bradley 5 September 2003 (has links)
No description available.
50

Cryptosporidium studies: maintenance of stable populations through in vivo propagation and molecular detection strategies

Ramirez, Norma E 18 March 2005 (has links)
No description available.

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