Thesis (M.Med.(Haematology))--University of the Witwatersrand, Faculty of Health Sciences, 1992.

Thesis (M.S.)--Case Western Reserve University, 2008. / [School of Medicine] Department of Pathology. Includes bibliographical references.

Thesis (MTech (Medical Technology))--Cape Technikon, Cape Town, 2002 / Erythropoietic response is the first indication of bone marrow recovery following bone marrow or peripheral blood stem cell transplantation. Manual reticulocyte counting has not only proven to be outdated but an extremely crude method of analysis, particularly if accurate and reliable means of assessing erythroid response is required to assess bone marrow recovery. Automated methods allow for the quantification of maturation within each reticulocyte, by measuring the amount of RNA present. The method of choice for our reticulocyte analysis was the Reticulocyte Maturation Index (RMI). The RMI was obtained by dividing the number of immature reticulocytes counted by the total number of reticulocytes counted producing a reportable value of International Units (IU). A normal Reticulocyte Maturation Index is 0.20 to 0.50 IU. The aim of the study was multifold. We wanted to prove that the Reticulocyte Maturation Index (RMI) is indeed the fastest means to assess bone marrow recovery in various types of transplants, including Bone Marrow Transplant (BMT) and Peripheral Blood Stem Cell Transplant (PBSCT). We also wanted to draw comparisons between allogeneic and autologous transplants, as well as further assessing different disease types. This was done by measuring the Reticulocyte Maturation Index (RMI), Absolute Neutrophil Count (ANe) and the Platelet Count (PLT) within the various groups. We further wanted to assess the effect of preconditioning treatment, Mononuclear Counts (MNC) and Colony Forming Unit - Granulocyte and Monocyte Counts (CFU-GM) on the early RMI response. These comparisons resulted in a need to establish a working range to determine patients response therein, and final outcome of the transplants. Finally we wanted to establish whether the "day 14" marrow biopsy is necessary, particularly if the three peripheral blood parameters, RMI, ANC and PLT were used as routine procedure following transplantation. The Reticulocyte Maturation Index (RMI) was measured on the Coulter EPICS ProfIle II flow cytometer; the ANC and PLT were measured on the Technicon H2 Haematology System. All other results such as the Mononuclear Counts (MNC), Colony Forming Unit - Granulocyte and Monocyte counts (CFU-GM), "day 14" and "day 28" bone marrow biopsies were retrieved from laboratory records. Forty nine transplant patients were evaluated for RMI over a period of six months, at the Department of Haematology, Groote Schuur Hospital, Cape Town. Four patients failed to engraft; and were not used in the calculations; but were evaluated as an aspect of the study in the final analysis. Forty five patients were analysed to establish the values used in the study, these patients were divided into eleven groups.

Orientador: André Fattori / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-23T14:36:17Z (GMT). No. of bitstreams: 1 Silva_JoaoCarlose_M.pdf: 3394200 bytes, checksum: 8d2a029f1ee7e0d42f602adc20337a62 (MD5) Previous issue date: 2013 / Resumo: Estudos demonstram a importância da anemia no contexto da senilidade, tanto por sua relação com perdas funcionais e redução da sobrevida, como também por ser identificada como um fator adicional para o desenvolvimento do fenótipo. De fato, a fragilidade e a anemia em indivíduos idosos parecem compartilhar alguns eventos fisiopatogênicos comuns relacionados ao processo inflamatório crônico. Doenças associadas ao envelhecimento, como aterosclerose, diabetes, doenças renais, alterações cardiovasculares e osteodegenerativas cursam com inflamação crônica e podem ter o seu impacto clínico agravado pelaconcomitância com a anemia. Os modelos mais utilizados no entendimento da fisiopatologia da fragilidade baseiam-se na interpretação da síndrome como resultado do acúmulo de déficits ao longo da vida33, resultando em maior vulnerabilidade biológica ou, ainda, como um estado de decréscimo do metabolismo global do individuo, associado a desregularão neuroendócrina e imunológica, que, também neste caso, cursa com redução da capacidade homeostática. Neste trabalho sugerimos que as alterações hematimétricas contribuem para o fenótipo como um agente estressor, mas também, representa um componente equivalente das perdas relacionadas à sarcopenia no modelo proposto por Friedet al. OBJETIVO GERAL:Este trabalho foi conduzido usando os dados do estudo multicêntrico populacional sobre fragilidade designada pela sigla FIBRA, acrônimo de Fragilidade em Idosos Brasileiros, em uma amostra de 255 idosos recrutados no domicilio, na urbana de Campinas. O fenótipo de fragilidade foi determinado utilizando os critérios do Cardiovascular Health Study e no Women Health andAgingStudy, já demonstrados e validados em publicações anteriores; os pontos de corte das variáveis de fragilidade, ajustados para a população brasileira, foram anteriormente publicados por nosso grupo.As amostras de sangue foram coletadas por de punção venosa utilizando material estéril, e acondicionadas protegidas da luz em tubo seco e tubo de EDTA. O material foi encaminhado ao laboratório de Hematologia do Hospital Universitário da Unicamp para a realização de hemograma e contagem de reticulócitos. Resultados: No modelo I da regressão multivariada, no qual foram incluídas apenas as variáveis hematimétricas, a classificação geral de fragilidade esteve relacionada à idade e o nível de HGB sérica; a contagem de RETAbs (contagem de reticulocitos absolutos) apareceu associada aos critérios individuais perda ponderal e atividade física (dados não mostrados em tabelas). No modelo II, no qual se introduziram as quantificações de citocinas inflamatórias, foram selecionadas como variáveis significativas a idade e a PCRus (proteína C reativa ultra sensível), sendo que cada um ano de avanço etário representou aumento de 1,20 vezes na chance de ser frágil, e cada aumento de 1 unidade no nível de PCRus sérica significou uma elevação de 5,56 vezes na chance de apresentar o fenótipo. Também no modelo II da análise multivariada verificou-se que para a diminuição de 10x106/mL na contagem de RETAbs, o grupo apresentou aumento de 40% na chance de ser frágil, enquanto que a cada elevação de 10x106/mL na contagem de RETAbs houve também aumento de 30% da chance de ser frágil. CONCLUSAO: estes achados sugerem que no conjunto de fragilidade, queda da concentração de hemoglobina, diminuição da contagem absoluta de reticulócitos e níveis séricos de PCRus talvez devam ser compreendidos como um componente constitutivo da fragilidade, estreitamente correlacionada ao processo de sarcopenia evidenciado pela perda ponderal. Neste sentido, os valores reduzidos dos índices hematimétricos, não somente indicam a participação da baixa oxigenação tecidual no processo fisiopatogênico da fragilidade, como também podem ser utilizados como mais um critério definidor do fenótipo. Este estudo FIBRA indica que futuras pesquisas de caráter longitudinal possam indicar os valores de hemoglobina e de reticulócitos absolutos que possam ser utilizados como critério definidor de fragilidade / Abstract: Studies have shown the importance of anemia in the context of senility both because of its relationship with functional loss and reduced survival and because it has been identified as an additional factor leading to the development of the phenotype. In fact, frailty and anemia in the elderly appear to share certain common pathophysiological events related to chronic inflammatory processes. Diseases associated with aging, such as atherosclerosis, diabetes, kidney disease, cardiovascular changes and osteodegenerative diseases, progress with chronic inflammation and their clinical impact may be aggravated if they occur concomitantly with anemia. The models most commonly used to understand the pathophysiology of frailty are based on an interpretation of the syndrome as the result of an accumulation of deficits during an individual's life, resulting in greater biological vulnerability or even a reduction in an individual's overall metabolism associated with neuroendocrine and immune dysregulation, which in this case is also accompanied by reduced homeostatic capacity19,34. We suggest that abnormal red cell indices contribute to the phenotype as a stressor but also play a similar role to that of sarcopenia in the model proposed by Fried et al., 2001. GENERAL OBJECTIVE: The study data were collected from a sample of residents in urban Campinas as part of the FIBRA (Frailty in Brazilian Seniors) multicenter population-based study. The frailty phenotype was determined using the criteria in the Cardiovascular Health Study and the Women's Health and Aging Study, which were described and validated in previous publications; the cut-off points for frailty variables adjusted for the Brazilian population were published previously by our group. Blood samples were collected by venipuncture using sterile material and stored away from light in dry collection tubes and EDTA tubes. The samples were sent to the Hematology Laboratory at Unicamp University Hospital for complete blood counts and reticulocyte counts. RESULTS: In multivariate regression model I, which included only red cell variables, the general frailty classification was related to age and serum hemoglobin (Hb) level. Absolute reticulocyte count (RetAbs) was associated with the individual criteria weight loss and reduced physical activity (data not shown in the tables). In model II, which also included inflammatory cytokine levels, age and hsCRP (high sensible C Reactive Protein) were selected as significant variables. Each additional year of age represented a 1.2-fold increase in the chance of being frail, and each 1-unit increase in serum hsCRP represented a 5.56-fold increase in the chance of having the frailty phenotype. Multivariate analysis using model II revealed that a reduction of 10x106/mL in RetAbs count resulted in a 40% increase in the chance of being frail, and that each 10x106/mL increase in this parameter also led to an increase (30%) in the chance of being frail. CONCLUSION: Our findings suggest that reduced Hb (hemoglobin) concentration, reduced RetAbs count and elevated serum hsCRP levels should perhaps be considered components of frailty, which is closely correlated with sarcopenia, as evidenced by weight loss. In this context, reduced red cell indices not only indicate that poor tissue oxygenation plays a role in the pathophysiology of frailty but can also be used as another criterion to define the phenotype. This FIBRA study has shown that future longitudinal studies may help determine Hb levels and RetAbs counts that can be used as criteria to define frailty / Mestrado / Gerontologia / Mestre em Gerontologia

A Fosfoetanolamina Sintética (FO-S), monoester-fosfolípide tem importante papel sobre a proliferação celular, indução da apoptose, em células tumorais, sem, contudo, afetar as células normais. Neste estudo foi avaliado o comportamento biológico e efeitos da toxicidade aguda da fosfoetanolamina sintética (FO-S), em experimentos de dose única e repetidas em camundongos sadios, contribuindo para validação pré-clínica. Os camundongos Balb-c de ambos os sexos receberam o composto FO-S via endovenosa nas doses de 50, 100, 250, 500 e 1000 mg/kg em dose única, e 50, 100 e 250 mg/kg em doses repetidas. No grupo dose única os animais que receberam 500 e 1000 mg/kg de FO-S apresentaram sinais de toxicidade, tais como: mortalidade de 33% dos animais; rebaixamento no sistema nervoso central e autônomo; flutuações das análises hematológicas; aumento dos níveis de TGO e TGP; diminuição de creatinina; análises da medula óssea mostraram diminuição das populações mieloides e linfoides; diminuição de células na fase G0/G1 do ciclo celular, assim como na fase S, e aumento na fase G2/M; alterações histológicas no coração, fígado e rins como necrose, esteatose, hialinização e hiperemia respectivamente. Tanto no grupo dose única, como no grupo doses repetidas, ocorreu aumento no número de reticulócitos no 7º dia após a aplicação, como resposta da medula óssea reativa, e as análises da sua celularidade revelaram atividade positiva do potencial elétrico mitocondrial. No grupo doses repetidas, os animais que receberam 50 mg/kg de FO-S apresentaram no 14º anemia leve, o grupo 100 mg apresentou aumento no número de leucócitos e linfócitos e o grupo 250 mg flutuações nos valores quantitativos de plaquetas, que retornaram à normalidade após 14 dias. As análises da medula óssea revelaram aumento de células no compartimento mieloide no grupo que recebeu 50 mg e aumento da celularidade no compartimento eritroide. A expressão dos marcadores de células precursoras hematopoiéticas da medula óssea, CD34, se mostrou aumentada no grupo que recebeu 250 mg aos 14 dias e diminuição do marcadores de células mielódes e subtipos de linfócitos, CD43, nos grupos que receberam 100 e 250 mg/kg aos 7 e 14 dias. Os animais que receberam 250 mg/kg apresentaram alterações no parênquima pulmonar. A análise de autofagia por citometria de fluxo que não revelou resposta negativa, como estresse oxidativo, apresentando produção normal de vacúolos autofágicos, assim como o teste de presença de micronúcleos não demonstrou danos às células por alterações genéticas induzidas por toxicidade / Synthetic Phosphoethanolamine (FO-S), a monoester phospholipid has an important role on cell proliferation, induction of apoptosis, in tumor cells, without, however, significantly affecting normal cells. In this study the biological behavior and effects of acute toxicity of synthetic phosphoethanolamine (FO-S) were evaluated in single dose and repeated experiments in healthy mice, contributing to the pre-clinical validation of this compound as antitumor phospholipid. Mice of both sexes received intravenous FO-S compound at doses of 50, 100, 250, 500 and 1000 mg / kg in single dose, and 50, 100 and 250 mg / kg in repeated doses. In the single dose group, animals receiving 500 and 1000 mg / kg FO-S showed signs of toxicity, such as: 33% mortality of animals; lowering in the central and autonomic nervous system; fluctuations in hematological analyzes; increased levels of TGO and TGP; decreased creatinine; bone marrow analysis showed decreased myeloid and lymphoid populations; decrease of cells in the G0 / G1 phase of the cell cycle, as well as in the S phase, and increase in the G2 / M phase; histological changes in the heart, liver and kidneys such as hyperemia, necrosis and hyalinization. In both the single dose and repeated dose groups, there was an increase in the number of reticulocytes on the 7th day after application, as a response of the reactive bone marrow, and the cellularity analysis showed positive mitochondrial electrical potential activity. In the repeated dose group, animals receiving 50 mg / kg FO-S presented in the 14th mild anemia, the 100 mg group showed an increase in the number of leukocytes and lymphocytes and the group 250 mg fluctuations in the quantitative platelet values, which returned to the normality at 14 days. Bone marrow analysis revealed increased cell count in the myeloid compartment in the 50 mg group and increased cellularity in the erythroid compartment. Expression of CD34 marrow hematopoietic precursor cell markers was shown to be increased in the 250 mg group at 14 days and a decrease in myelodystic cell markers and CD43 lymphocyte subtypes in the groups receiving 100 and 250 mg / kg at 7 and 14 days. Animals that received 250 mg / kg presented changes in the lung parenchyma. Autophagy analysis was performed by flow cytometry that revealed no negative response, such as oxidative stress, presenting normal production of autophagic vacuoles, as well as the presence of micronuclei test did not demonstrate damage to the cells by genetic changes induced by toxicity

Cells often exist in heterogeneous mixtures. Density provides a property to separate several types of cells from the mixed sample in which they originate. Density-based separation methods provide a standard method to quickly separate or enrich specific populations of cells, such as lymphocytes from whole blood. This dissertation explores the use of aqueous multiphase systems (AMPS) as self-forming step-gradients in density for the separation of cells. AMPS were first discovered over a hundred years ago as aqueous two-phase systems. Density as a tool to separate cells is at least as old. Despite this long history, the work in this thesis is the first work to use AMPS to perform density-based separations on cells. This combination provides a powerful technique to separate cells and enable new testing at the point-of-care. Chapter 1 provides a short overview of aqueous multiphase systems and density-based separations of cells. Chapter 2 describes the process of taking technology, including AMPS, from a demonstration in a laboratory to a large scale evaluation in a field setting. In Chapter 3 and Appendix I, AMPS provide a means to enrich reticulocytes from whole blood as a means to grow malaria parasites. Chapter 4 and Appendix II describe the development and proof-of-prinicple of a density-based diagnostic test for sickle cell disease (SCD) using AMPS. Chapter 5 and Appendix III detail the results of a large scale field evaluation of a rapid test for SCD using AMPS in Zambia. Demonstrations of AMPS for density- and size-based separations are provided in Appendices IV and V. Appendix VI demonstrates the general usefulness of density to separate crystal polymorphs with another density-based separation method: magnetic levitation in a paramagnetic fluid. Beyond density, novel combinations of technology, such as electrochemistry and telecommunications provide opportunities for enabling global health (Appendix VII). / Engineering and Applied Sciences

Utilization of iron by hemoglobin-producing cells is highly efficient. The acquisition of iron from plasma requires the binding of diferric transferrin (Tf) to its cognate receptor (Tf-R) on the erythroid cell membrane, followed by internalization of the Tf - Tf-R complexes via receptor-mediated endocytosis. Through a poorly understood mechanism, iron is targeted to mitochondria, the site of heme biosynthesis. We believe that a direct interaction between iron-containing endosomes and mitochondria is essential for iron transfer to mitochondria and its efficient incorporation into heme. / In order to illustrate the interaction between endosomes and mitochondria, we have employed flow cytometry. Flow cytometry analysis of reticulocytes (erythrocyte precursors which still synthesize hemoglobin) stained with fluorescent dyes specific to mitochondria and endosomes revealed three distinct populations: mitochondria, endosomes and a population labeled with both dyes. This double-labeled population suggests a population composed of endosomes associated with mitochondria. Using non-fluorescent diferric-Tf, we were able to remove the double population, leaving only the endosomal and the mitochondrial population. This finding has confirmed that the double population is the result of the interaction between the two organelles. / Additionally, we established a cell-free assay consisting of fluorescent mitochondria and endosomes isolated from erythroid cells. Using confocal microscopy, we demonstrated a colocalization between the two organelles. We repeated the assay using fluorescent mitochondria and endosomes isolated from HeLa spinner cells. Using the mitochondrial uncoupler CCCP, we were able to significantly reduce the colocalization between the two organelles, indicating that the interaction between the organelles is specific and that the mitochondrial potential is a requirement for organellar interaction. / Based on our results from flow cytometry and confocal microscopy, we conclude that a specific and direct interaction exists between the two organelles.

With the sharp increase on the demand for lamb meat, Brazilian sheep industry has experienced an unprecedented growth on the national flock. Among the limiting factors in sheep production anemia due to worm infection is a major cause of losses. The objective of the present work was to evaluate the oxidative status and the red blood cell profile in lambs with bleeding induced anemia, supplemented or not with dextran iron. Ten 5 to 7 month-old ram lambs were used. Day zero was defined when each experimental animal attained 15% packed cell volume after successive bleedings. Animals were then allocated into 2 experimental groups: Treated group which received a single injection of dextran iron (25 mg/kg i.m.) and Control animals that received no treatment. Blood samples were collected on days zero, 7, 14 and 21. At days 7 and 21 there was an increase in the tiobarbituric acid reactive species (TBARS) in treated animals. The same group presented reduced levels of non-protein Thiol groups (NPTH) at days 7, 14 and 21. At day 7 osmotic fragility of red blood cells was increased in control animals as compared to the treated group. Red blood cell profile recovered faster in treated than control animals. In conclusion, iron supplementation eventhough inducing oxidative stress accelerated the recovery of hematological profile. The results can suggest the use of iron injections in conjunction with wormers in the treatment of haemonchus infections / A potencialidade do mercado de carne ovina é uma realidade, devido a isto a ovinocultura brasileira tem se desenvolvido de maneira bastante acentuada. Entre os fatores limitantes para a produção de ovinos a anemia verminótica é a principal causa de prejuízos para os produtores. O objetivo deste trabalho foi avaliar o status oxidativo e a recuperação do quadro hematológico de cordeiros com anemia induzida por sangramento e suplementados ou não com ferro. Foram utilizados 10 cordeiros machos entre 5 e 7 meses de idade. O dia zero do experimento foi considerado quando cada animal atingiu o hematócrito 15% após a indução da anemia e foi alocado em um dos grupos experimentais. O grupo tratado recebeu uma injeção de 25 mg/kg de peso vivo de ferro dextrano intramuscular em dose única e o grupo controle não foi tratado. As coletas de sangue foram feitas nos dias zero, 7, 14 e 21. Nos dias 7 e 21 após ao tratamento houve aumento nos valores de espécies reativas ao ácido tiobarbitúrico (TBARS) e diminuição nos valores dos grupamentos tióis não protéicos (NPTH) nos dias 7, 14 e 21 no grupo tratado. No dia 7, o teste de fragilidade osmótica eritrocitária indicou um aumento na resistência da membrana dos eritrócitos dos animais do grupo tratado quando comparado aos animais do grupo controle. A recuperação da série vermelha do sangue (eritrócitos, hematócrito e hemoglobina) foi mais rápida no grupo tratado. A suplementação de ferro apesar de aumentar o estresse oxidativo acelerou a recuperação do quadro hematológico, não aumentando a porcentagem de hemólise pela ação das espécies reativas de oxigênio sobre a membrana eritrocitária. Pode-se sugerir também apartir destes resultados a utilização de ferro como tratamento auxiliar para verminose junto com anti-helmínticos

A Fosfoetanolamina Sintética (FO-S), monoester-fosfolípide tem importante papel sobre a proliferação celular, indução da apoptose, em células tumorais, sem, contudo, afetar as células normais. Neste estudo foi avaliado o comportamento biológico e efeitos da toxicidade aguda da fosfoetanolamina sintética (FO-S), em experimentos de dose única e repetidas em camundongos sadios, contribuindo para validação pré-clínica. Os camundongos Balb-c de ambos os sexos receberam o composto FO-S via endovenosa nas doses de 50, 100, 250, 500 e 1000 mg/kg em dose única, e 50, 100 e 250 mg/kg em doses repetidas. No grupo dose única os animais que receberam 500 e 1000 mg/kg de FO-S apresentaram sinais de toxicidade, tais como: mortalidade de 33% dos animais; rebaixamento no sistema nervoso central e autônomo; flutuações das análises hematológicas; aumento dos níveis de TGO e TGP; diminuição de creatinina; análises da medula óssea mostraram diminuição das populações mieloides e linfoides; diminuição de células na fase G0/G1 do ciclo celular, assim como na fase S, e aumento na fase G2/M; alterações histológicas no coração, fígado e rins como necrose, esteatose, hialinização e hiperemia respectivamente. Tanto no grupo dose única, como no grupo doses repetidas, ocorreu aumento no número de reticulócitos no 7º dia após a aplicação, como resposta da medula óssea reativa, e as análises da sua celularidade revelaram atividade positiva do potencial elétrico mitocondrial. No grupo doses repetidas, os animais que receberam 50 mg/kg de FO-S apresentaram no 14º anemia leve, o grupo 100 mg apresentou aumento no número de leucócitos e linfócitos e o grupo 250 mg flutuações nos valores quantitativos de plaquetas, que retornaram à normalidade após 14 dias. As análises da medula óssea revelaram aumento de células no compartimento mieloide no grupo que recebeu 50 mg e aumento da celularidade no compartimento eritroide. A expressão dos marcadores de células precursoras hematopoiéticas da medula óssea, CD34, se mostrou aumentada no grupo que recebeu 250 mg aos 14 dias e diminuição do marcadores de células mielódes e subtipos de linfócitos, CD43, nos grupos que receberam 100 e 250 mg/kg aos 7 e 14 dias. Os animais que receberam 250 mg/kg apresentaram alterações no parênquima pulmonar. A análise de autofagia por citometria de fluxo que não revelou resposta negativa, como estresse oxidativo, apresentando produção normal de vacúolos autofágicos, assim como o teste de presença de micronúcleos não demonstrou danos às células por alterações genéticas induzidas por toxicidade / Synthetic Phosphoethanolamine (FO-S), a monoester phospholipid has an important role on cell proliferation, induction of apoptosis, in tumor cells, without, however, significantly affecting normal cells. In this study the biological behavior and effects of acute toxicity of synthetic phosphoethanolamine (FO-S) were evaluated in single dose and repeated experiments in healthy mice, contributing to the pre-clinical validation of this compound as antitumor phospholipid. Mice of both sexes received intravenous FO-S compound at doses of 50, 100, 250, 500 and 1000 mg / kg in single dose, and 50, 100 and 250 mg / kg in repeated doses. In the single dose group, animals receiving 500 and 1000 mg / kg FO-S showed signs of toxicity, such as: 33% mortality of animals; lowering in the central and autonomic nervous system; fluctuations in hematological analyzes; increased levels of TGO and TGP; decreased creatinine; bone marrow analysis showed decreased myeloid and lymphoid populations; decrease of cells in the G0 / G1 phase of the cell cycle, as well as in the S phase, and increase in the G2 / M phase; histological changes in the heart, liver and kidneys such as hyperemia, necrosis and hyalinization. In both the single dose and repeated dose groups, there was an increase in the number of reticulocytes on the 7th day after application, as a response of the reactive bone marrow, and the cellularity analysis showed positive mitochondrial electrical potential activity. In the repeated dose group, animals receiving 50 mg / kg FO-S presented in the 14th mild anemia, the 100 mg group showed an increase in the number of leukocytes and lymphocytes and the group 250 mg fluctuations in the quantitative platelet values, which returned to the normality at 14 days. Bone marrow analysis revealed increased cell count in the myeloid compartment in the 50 mg group and increased cellularity in the erythroid compartment. Expression of CD34 marrow hematopoietic precursor cell markers was shown to be increased in the 250 mg group at 14 days and a decrease in myelodystic cell markers and CD43 lymphocyte subtypes in the groups receiving 100 and 250 mg / kg at 7 and 14 days. Animals that received 250 mg / kg presented changes in the lung parenchyma. Autophagy analysis was performed by flow cytometry that revealed no negative response, such as oxidative stress, presenting normal production of autophagic vacuoles, as well as the presence of micronuclei test did not demonstrate damage to the cells by genetic changes induced by toxicity

Made available in DSpace on 2016-01-26T18:55:36Z (GMT). No. of bitstreams: 1 Paulo Henrique da Silva.pdf: 146595 bytes, checksum: b33ac213ba3a976522fc7a41aa05e514 (MD5) Previous issue date: 2013-08-05 / Changes in the results of erythrocyte standards require decisions and guide the conduct of clinical regarding prognosis and treatment of any diseases. The use of modern equipment that make the blood cell count used to calculate the RDW (red cell distribution width) and its fractions RDW-CV and RDW-SD, which provide a quantitative assessment of heterogeneity of each erythrocyte. Given the limited information available in the literature about the RDW for the domestic cat, this study aims to compare the values of RDW, with MCV and reticulocytes from 40 (n=14males, n=26 females) healthy mongrel cats, with age ranging from 1.2 to 5.5 years (3.0 ± 1.2) were used. The animals were kept in individual cages all over the experiment. Blood samples were taken directly from the external jugular vein and separated into two tubes: one containing anticoagulant EDTA (ethylene diamine tetra acetic acid) to 10% for the complete blood count and the second without anticoagulant for biochemical tests. The results showed that there is a direct relationship between values of RDW-SD and RDW-CV and an inverse correlation between RDW-CV with MCV. There was no relation between RDW and reticulocyte aggregates or punctate. There was no relation between sex and RDW as well. RDW values determined in this experiment can be used as a resource for future research and also as reference values for the feline species. / As alterações nos resultados dos padrões eritrocitários demandam decisões e norteiam a conduta do clínico quanto ao prognóstico e a terapêutica de eventuais patologias. A utilização de equipamentos modernos que fazem a contagem celular sanguínea permite calcular a amplitude de distribuição dos eritrócitos ou RDW (red cell distribution width) e suas frações RDW-CV e RDW-SD, que provêem uma avaliação quantitativa da heterogenicidade de cada eritrócito. Diante da escassa informação disponível na literatura científica sobre os valores de RDW para o gato doméstico, este estudo se propõe a comparar os valores de RDW com o VCM e reticulócitos de 40 gatos domésticos hígidos, sem raça definida, com idade variando de 1,2 a 5,5 anos (3,0±1,2), sendo 14 machos e 26 fêmeas provenientes do gatil da instituição. O sangue foi colhido diretamente da veia jugular externa e separado em dois tubos: um contendo anticoagulante EDTA (ácido etileno diamino tetra acético) a 10%, para a realização do hemograma e o segundo, sem anticoagulante, para as provas bioquímicas de triagem. Os resultados encontrados mostraram que existe uma relação direta significativa entre os valores de RDW-SD e RDW-CV e uma correlação inversa entre os valores de RDW-CV o VCM. Não houve correlação entre os valores de RDW e reticulócitos agregados ou ponteados. O sexo não interfere no diâmetro das hemácias. Os valores de RDW determinados neste experimento podem ser usados como fonte de consulta para futuras pesquisas e também como valores de referência da espécie felina.