Thesis (Ph. D.)--University of Nebraska.

Thesis (Ph. D.)--University of Nebraska. / Includes bibliographical references.

Female Wistar strain rats were swim trained six hours/day, five days/week, for eight weeks. Animals were sacrificed after 2, 4, 6, or 8 weeks of training, or after 8 weeks of training followed by 7, 14, or 21 days of detraining. The red vastus and soleus muscles were removed and assayed for succinate dehydrogenase (SDH) levels.. Sedentary rats were sacrificed during the detraining time period to provide control values of SDH activity in the two muscles. The SDH values of the red vastus muscle were significantly higher than those obtained from the controls after eight weeks of training, but higher in the soleus by six weeks of training. These elevated levels of SDH were maintained for at least seven days after training ceased in both muscles. By the 14th day of detraining, SDH levels of both muscles had dropped drastically, but were still significantly higher than control values. After 21 days of detraining the SDH levels of the two muscles were only slightly lower than those obtained after 14 days of detraining.The results indicate that:1) there is no loss in the training-induced increased oxidative capacity of the muscle following seven days of inactivity, and 2) there was a significant loss in this training effect by 14 days, though a total loss was not observed even after 21 days of detraining. These results should be of interest to people who train at a moderate intensity and must miss a series of training sessions due to injury or illness.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1966. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1967. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Succinic Acid (SA) is an intermediate in the production of fine and commodity chemicals. No commercial SA bioproduction process exists due to process limitations including end product inhibition and high product separation costs, which account for 70% of total production costs. Two-Phase Partitioning Bioreactors (TPPBs) can increase volumetric productivity through in-situ product removal, although SA uptake by polymers requires a pH below the pKA2 of SA (4.2). Sparging CO2 gas into the bioreactor was proposed to temporarily lower the pH of the medium, allowing for SA uptake. At 1atm CO2 sparging lowered the pH of Reverse Osmosis (RO) water to 3.8 but only to 4.75 in medium, requiring the use of H2SO4 and KOH for pH adjustment in subsequent experiments. Polymers were screened for SA uptake and the effect of pH on uptake from 2.2 to 6.2 was also studied. Only Hytrel® 8206 showed non-zero uptake with a partition coefficient for SA of 1.3. Cell cultures of Actinobacillus succinogenes was exposed to pH 4.2 for times from 5 minutes to 4 hours to determine whether cells could grow after low pH exposure. A. succinogenes resumed growth after up to 4 hours of low pH exposure, giving a sufficient time span for SA uptake in the bioreactor. A single-phase run was operated as a benchmark for comparison to the TPPB system which removed SA from the fermentation broth by pH cycling; lowering the pH to 3.8 for uptake, then increasing it to 6.7 to continue bioproduction. Uptake from fermentation broth took 60 minutes, within the time causing no effect on cell growth from low pH exposure. The two-phase run yielded 1.39g/L•h, unchanged compared to the single-phase run which gave 39g/L of SA after 28 hours. Though pH cycling reduced the concentration of SA through polymer uptake, the salts added for pH adjustment hindered further cell growth. The TPPB system demonstrated that SA can be efficiently removed from solution without complex separation methods. Future work will use pressurized vessels to increase the solubility of CO2 and lower the pH of fermentation broth for SA uptake without the need for strong acids. / Thesis (Master, Chemical Engineering) -- Queen's University, 2011-04-18 08:07:51.379

Thesis (Ph. D.)--University of Wisconsin--Madison, 1965. / Typescript. Abstracted in Dissertation abstracts, v. 25 (1965) no. 10, p. 5575-76. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Inaug.-diss.--Strassburg.

Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 97-102).

No description available.