Desenvolvemos dois modelos estatísticos para água, nos quais diferentes aspectos de sua estrutura são considerados. O modelo geométrico permite diferentes números de coordenação. Em uma aproximação de campo médio mostramos que sob pressão o modelo apresenta linha de coexistência entre fases de baixa e alta densidade, e ponto crítico. A entropia das ligações de hidrogênio tem papel fundamental na definição da inclinação da linha de coexistência. O comportamento do modelo pode estar realcionado como segundo ponto crítico da água super resfriada e com transições líquido-líquido em geral.O modelo da água quadrada é uma versão térmica do modelo do gelo, no qual considera-se a direcionalidade das ligações de hidrogênio. O modelo foi estudado na rede de Bethe e através de simulações de Monte Carlo em três situações diferentes: para a água pura e na presença de solutos polares e apolares. A água quadrada pura não apresenta transição de fase. No modelo para solvente com solutos apolares, medidas de frequência relativa de ligações e do tempo de correlação mostram que o modelo apresenta estruturação da camada de hidratação. Medidas de correlação temporal no modelo de Ising mostram comportamento oposto. Em um estudo preliminar para uma solução com solutos que realizam ligações de hidrogênio não conseguimos encontrar diagramas de coexistência com círculo fechado, para o conjunto de parâmetros utilizados, possivelmente devido à ausência de buracos. / We have developed two statistical models for water in which different features of water structure are considered. In the geometrical model different coordination numbers are present and the model allows for translational disorder. A mean-field treatment shows that under pressure the model exhibits phases of different densities and a coexistence line ending in a critical point. Entropy of the hydrogen network plays an essential role in defining the slope of the coexistence line. The model behavior might be related with the second critical point in supercooled water and to liquid-liquid transitions in general. The square water model is a thermal version of the ice model, and takes into account the directionality of the hydrogen bonds. The model was studied on a Bethe lattice and through Monte Carlo simulations, for three different situations: as pure water and in the presence of polar and apolar solutes. Pure square water does not present a phase transition. In the presence of apolar solutes, first shell square water presents ordering, as shown from comparison of relative frequency of bonds, as well as from study of time correlations. The latter was shown to present opposite behavior in case of an Ising system. In a preliminary study for a solution of hydrogen bonding solutes we were unable to find a closed loop for the sets of parameters chosen. Vacancies might need to be included.

The mammalian hippo signalling pathway controls cell proliferation and apoptosis via transcriptional co-activators YAP and TAZ, and as such is a key regulator of organ and tissue growth. Multiple cellular components converge in this pathway, including the actin cytoskeleton, which is required for YAP/TAZ activity. The precise mechanism by which the mechanical actomyosin network regulates Hippo signalling, however, is unknown. Optical methods provide a non-invasive way to image and study the biomechanics of cells. In the past two decades, super-resolution fluorescence microscopy techniques that break the diffraction limit of light have come to the fore, enabling visualisation of intracellular detail at the nanoscale level. Optical trapping, on the other hand, allows precise control of micron-sized objects such as cells. Here, super resolution structured illumination microscopy (SIM) and elastic resonator interference stress microscopy (ERISM) were used to investigate a potential role for the FERM-domain protein FRMD6, or Willin, in the mechanical control of the Hippo pathway in a neuronal cell model. A double optical trap was also integrated with the Nikon-SIM with the aim of cell stretching. Willin expression was shown to modify the morphology, neuronal differentiation, actin cytoskeleton and forces of SH-SY5Y cells. Optical trapping from above the SIM objective, however, was demonstrated to be ineffective for manipulation of adherent cells. The results presented here indicate a function for Willin in the assembly of actin stress fibres that may be the result of an interaction with the Hippo pathway regulator AMOT. Further investigation, for example by direct cell stretching, is required to elucidate the exact role of Willin in the mechanical control of YAP/TAZ.

Os compostos de origem microbiana tem readquirido interesse pela biodisponibilidade, especificidade de alvo e diversidade química, mas as vias biosintéticas permanecem crípticas em condições de cultura. Uma estratégia para expressa-las é a super-expressão de genes ativadores. O laboratório de Bio-Produtos no ICB na USP tem trabalhado com Streptomyces olindensis produtor da Cosmomicina D uma molécula com atividade antitumoral de interesse devido ao padrão de glicosilação. O genoma de S. olindensis foi sequenciado e submetido ao NCBI (JJOH00000000) e utilizando o software antiSMASH foram identificados 33 clusters envolvidos na produção de metabolitos secundários. Encontraram-se clusters gênicos para a produção de metabolitos como Melanina, Geosmina, entre outros. Além, foi realizada uma analise de genômica comparativa para caracterizar e anotar as 22 vias biossintéticas desconhecidas em S. olindensis. Finalmente, escolheram-se a via do aminociclitol e um Policetídeo Tipo I para a super-expressão de genes reguladores levando a detecção do composto sob condições de cultura. / Microbial metabolites regain interest due to its bioavailability, target specificity and chemical diversity, but the biosynthetic pathways remain silenced under culture conditions. A strategy to obtain them is the over expression of regulatory genes. Bio-products laboratory at USP has been working with Streptomyces olindensis, products of Cosmomycin D, an antitumoral molecule with a distinctive glycosylation pattern. S. olindensis genome was sequenced and submitted to NCBI (JJOH00000000) and employing antiSMASH server 33 secondary metabolite related clusters were identified. Known pathways were found such as genes for melanin production, Geosmin and others. Additionally, a comparative genomic approach was used to characterize the 22 biosynthetic unknown pathways described in S. olindensis. Subsequently, Aminocyclitol and Polyketide Type I were chosen to evaluated, over expressing the regulatory genes, leading to the compound detection in regular culture conditions.

A necessidade de transmissão de canais modulados a taxas a partir de 400 Gb/s tem motivado a pesquisa e os esforços relativos às tecnologias de camada física habilitadores desta alta capacidade. A atenção se volta, principalmente, aos frontends (transmissores e receptores), aliados aos processadores digitais de sinal (Digital Signal Processors, DSPs), às técnicas de amplificação óptica e a novos tipos de fibra óptica. Em particular a técnica baseada no emprego de filtros de Nyquist combinados à multiplexação de comprimentos de onda (Wavelength Division Multiplexing, DWM), conhecida como Nyquist-WDM, ou N-WDM, tem atraído grande interesse para geração de supercanais ópticos, hoje um dos elementos chave nos sistemas de redes ópticas. O estudo dos fundamentos e casos particulares dos filtros de Nyquist são aprofundados nesta dissertação para o controle de seus parâmetros, em especial o parâmetro conhecido como fator de roll-off, em aplicações que requerem flexibilidade na ocupação espectral e até o reaproveitamento das limitações do filtro para atenuar alguns efeitos lineares e não lineares na fibra. A técnica utiliza um tipo de formatação geométrica de pulso e é limitada pelo ajuste grosso do fator de roll-off, mas como abordagem inicial, permite estabelecer uma série de compromissos na concepção do circuito eletrônico de um transponder sintonizável. Uma investigação teórica foi feita em um sistema PM-16QAM de 21x256 Gb/s, a partir de dados experimentais obtidos com roll-off igual 0,1, para análise do efeito no desempenho sistêmico do ajuste do excesso de largura de banda (em relação à banda de Nyquist) de um filtro formatador de pulso. O fator de roll-off foi ajustado e seu impacto no desempenho do sistema, em termos de alcance, foi verificado. A partir dos resultados, foi observado que, desde que a taxa de erro de bit, BER, esteja dentro do limite do código corretor de erro (forward error corrector, FEC), o valor de roll-off pode ser ajustado para um valor ótimo de acordo com a configuração do sistema e as metas requeridas. Uma vez encontrada a relação entre a BER e o fator de roll-off, foi possível determinar um fator de mérito que relaciona a resolução do filtro de Nyquist, em função do número de taps que ele emprega, o consumo de energia da DSP e, consequentemente, a BER. O compromisso assim estabelecido entre o desempenho sistêmico, o consumo de energia e o fator de roll-off representa a principal contribuição desta dissertação. / The need for transmission of channels modulated at rates greater than 400 Gb/s has motivated the research and efforts related to the physical layer technologies that will enable this high capacity. The attention turns mainly to the frontends (transmitters and receivers), allied to digital signal processors (DSPs), optical amplification techniques and new types of optical fiber. The technique based on the use of Nyquist filters combined withWavelength Division Multiplexing (WDM), known as Nyquist-WDM, or N-WDM, has attracted great interest for the generation of optical super-channels, today one of the key elements in optical network systems. The study of the fundamentals and particular cases of the Nyquist filters are detailed in this dissertation for mastering the control of the parameters, especially the parameter known as roll-off factor, for applications that require flexibility in the spectral occupation and even the reutilization of the limitations of the filter to attenuate some linear and non-linear effects on the fiber. The technique uses a geometric type of pulse-shaping, and is limited by the roll-off factor tunning, but as an initial approach, it allows to establish a series of compensations in the design of the electronic circuit of a tunable transponder. A theoretical investigation was made on a 21x256 Gb/s PM-16QAM system, taken as reference the experimental data obtained with roll-off equal to 0.1, to analyze the effects of adjusting the excess bandwidth (relative to the Nyquist band) of a pulse-shaping filter. The roll-off factor was tunned and its impact on the system performance in terms of range effects was verified. From the results, it was observed that, as long as the bit error ratio, BER, is within the FEC limit, the roll-off parameter can be set to an optimum value according to the system configuration and required targets. Once the relationship between the BER and the roll-off factor was found, it was possible to determine a merit factor that relates the resolution of the Nyquist filter, as a function of the number of taps it uses, the energy consumption of the DSP and, consequently, the BER. The compromise thus established between system performance, energy consumption and roll off represents the main contribution of this work.

Submitted by Fabricia Fialho Reginato (fabriciar) on 2015-08-28T22:58:37Z No. of bitstreams: 1 CarolZANDAVALLI.pdf: 653303 bytes, checksum: 01703b6710ca7fa9d53fee01bbaaa8e3 (MD5) / Made available in DSpace on 2015-08-28T22:58:37Z (GMT). No. of bitstreams: 1 CarolZANDAVALLI.pdf: 653303 bytes, checksum: 01703b6710ca7fa9d53fee01bbaaa8e3 (MD5) Previous issue date: 2013 / Nenhuma / Nosso texto visa aprofundar a abordagem de Heidegger sobre o conceito de vontade de poder em Nietzsche e suas consequências. Para tanto, trataremos primeiramente de compreender alguns aspectos centrais do pensamento do próprio Heidegger. Esse preâmbulo inicial nos ajudará para uma melhor compreensão do contexto de onde parte a sua crítica. Em seguida, defrontarnos-emos com a interpretação heideggeriana da vontade de poder em modo mais estrito. Teremos, por fim, melhores condições de pensar a pertinência do que é apresentado por Heidegger, isto é, de sua visão sobre uma metafísica nietzschiana com bases nos conceitos de vontade de poder e eterno retorno do mesmo. / Our text aims to deepen Heidegger’s approach on the concept of will to power in Nietzsche and its consequences. To do so, we will try first to understand some central aspects of Heidegger's own thinking. This initial preamble will help us to have a better understanding of the context where his criticism comes from. Then we will face with the heideggerian interpretation of the will to power in a stricter mode. Finally, we will be more able to think about the relevance of what is presented by Heidegger, that is, of his vision on a Nietzschean metaphysics based on the concepts of will to power and eternal return of the same.

Ce texte étudie le groupe quantique Uξ sl(2|1) associé à la superalgèbre de Lie sl(2|1) et une catégorie de ses représentations de dimension finie. L'objectif est de construire des invariants topologiques de 3-variétés en utilisant la notion de trace modifiée. D'abord nous prouvons que la H catégorie CH des modules de poids nilpotents sur Uξ sl(2|1) est enrubannée et qu'il existe une trace modifiée sur son idéal des modules projectifs. De plus CH possède une structure relativement G-prémodulaire ce qui est une condition suffisante pour construire un invariant de 3-variétés à la Costantino-Geer-Patureau. Cet invariant est le cœur d'une 1+1+1-TQFT (Topological Quantum Field Theory). D'autre part Hennings a proposé à partir d'une algèbre de Hopf de dimension finie une construction d’invariants qui dispense de considérer la catégorie de H l l ses représentations. Nous montrons que le groupe quantique déroulé Uξ sl(2|1)/(e1 , f1 ) possède une complétion qui est une algèbre de Hopf enrubannée topologique. Nous construisons un invariant de 3-variétés à la Hennings en utilisant cette structure algébrique, une transformation de Fourier discrète et la notion de G-intégrales. L'intégrale dans une algèbre de Hopf est centrale dans la construction de Hennings. La notion de trace modifiée dans une catégorie s'est récemment révélée être une généralisation des intégrales dans les algèbres de Hopf de dimension finie. Dans un contexte plus général d'algèbre de Hopf de dimension infinie nous prouvons la relation formulée entre la trace modifiée et la G -intégrale. / This text studies the quantum group Uξ sl(2|1) associated with the Lie superalgebra sl(2|1) and a category of finite dimensional representations. The aim is to construct the topological invariants of 3-manifolds using the notion of modified trace. We first prove that the category CH of the nilpotent weight modules over Uξ sl(2|1) is ribbon and that there exists a modified trace on its ideal of projective modules. Furthermore, CH possesses a relative G-premodular structure which is a sufficient condition to construct an invariant of 3-manifolds of Costantino-Geer-Patureau type. This invariant is the heart of a 1+1+1-TQFT (Topological Quantum Field Theory). Next Hennings proposed from a finite dimensional Hopf algebra, a construction of invariants which does not require to consider the category of its representations. We show that the unrolled H l l quantum group Uξ sl(2|1)/(e1 , f1 ) has a completion which is a topological ribbon Hopf algebra. We construct an invariant of 3-manifolds of Hennings type using this algebraic structure, a discrete Fourier transform, and the notion of G-integrals. The integral in a Hopf algebra is central in the construction of Hennings. The notion of modified trace in a category has recently been revealed to be a generalization of the integrals in a finite dimensional Hopf algebra. In a more general context of infinite dimensional Hopf algebras we prove the relation formulated between the modified trace and the G-integral.

Les comic books, qui font partie du 9ème art avec la bande dessinée et le manga, constituent un pan important de la culture populaire et de l’imaginaire contemporain. Ils permettent d’attester de la persistance des super-héros, dont les aventures cycliques sont très appropriées à ce moyen d’expression. La variété des modes narratifs qui transmettent aujourd’hui les super-héros, depuis le cinéma jusqu’à nos univers virtuels contemporains, permet de questionner les enjeux de la fiction et sa vitalité. Ainsi les récits audio-visuels sont étudiés au même titre que ceux de papier.Cette multimodalité narrative de nos super-héros doit tenir compte de la vaste fresque que représentent leurs récits ; et ce sont des éléments représentatifs de mouvances éditoriales, historiques, ou encore sociales qui guident le choix de notre corpus d’étude, au travers des « Ages » de nos super-héros. L’imaginaire dynamique des personnages nous amène à retenir trois d’entre eux pour lesquels la multisensorialité et la moto-sensorialité sont l’essence même de leurs narrations. Cela permet de souligner l’apport des sciences cognitives dans l’analyse des ontologies de la fiction, de sorte que ces personnages intègrent parfaitement, en dépit des objections de Sperber & Hirschfeld (2004), les « fondations cognitives » de nos cultures. Nous défendons que les super-héros font partie des êtres fantastiques et nous utilisons pour leur analyse le cadre heuristique proposé dans Armand (2015), qui permet l’étude des êtres fantastiques repérés dans la Folkloristique en s’appuyant sur les acquis de l’Anthropologie Neuro-Cognitive. Ce cadre nous a permis d’analyser les pouvoirs multisensoriels de nos super-héros en termes d’intuitions, contre-intuitions et sur-intuitions, pour mieux approcher les mécanismes cognitifs qui fondent l’imaginaire des auteurs qui nous proposent ces héros tout autant que l’imaginaire de ceux qui y adhèrent.Batman, justicier costumé en chauve-souris, intègre dans sa lutte contre le crime les représentations de cet animal dans ce qu’il a de plus ambivalent dans nos imaginaires. Il reste toutefois incapable d’employer dans sa fiction une perception biologique naturelle du chiroptère : l’écholocation animale. Bien que connue du grand public, cette capacité demeure dans son fonctionnement trop contre-intuitive pour être représentée avec justesse dans les récits.Daredevil nous permet de poursuivre notre approche de la multisensorialité, avec le cas d’un justicier aveugle aux capacités perceptives et attentionnelles décuplées. Mais ce « super-aveugle » est incapable d’avoir recours à la compétence sur-intuitive de l’écholocation humaine, une compétence qui semble au final dépendre très contre-intuitivement des aires corticales visuelles plutôt que des aires auditives (depuis Thaler et al., 2011).Superman enfin possède a priori tous les pouvoirs, mais est particulièrement reconnu par sa capacité à voler sans contraintes. Cette capacité nous a conduit à rechercher dans quel incubateur neural d’ontologies fantastiques le récit puisait la composante génératrice de ce si célèbre pouvoir de vol aptère. Nous avons pour cela dû faire appel aux expériences hors-du-corps (Out-of-Body Experiences ou OBE), rapportées de longue date et universellement dans les narrations d’expérience, et dont l’émergence n’a été vraiment comprise sur le plan neural que récemment (cf. les expériences de stimulation corticale de Blanke et al., 2002). Nous proposons une approche qui permet d’unifier la Formation de l’esprit scientifique de Bachelard (1938), qui donne les bases d’une épistémologie de la contre-intuition, avec sa poétique. Car nous avons pu requalifier la poétique de L’Air et les songes (1943), dans ses apports fondamentaux − ici le vol onirique aptère −, de sur-intuitive. Ce vol sans ailes trouve par ailleurs un réel terrain d’expression par le mouvement imaginé dans les mondes virtuels et l’expérience de corps dissocié dans la Réalité Virtuelle. / Comic books constitute with comic albums and manga the 9th art and represent an important part of popular culture and contemporary imaginary. They bear witness of the persistence of superheroes, whose cyclic adventures are appropriate for this form of expression. The variety of narrative modes (from cinema to our contemporary virtual universes) that convey superheroes today allows us to question the issues of fiction and its vitality. Audio-visual narratives are thus as studied as paper narratives.The narrative multimodality of our superheroes must take into account the large fresco represented by their stories. These elements are representative of editorial, historical or even social movements that have guided the composition of our corpus throughout the “Ages” of ours superheroes. The dynamic imaginary of characters led us to select three of them for whom multisensoriality and motosensoriality are the very essences of their narratives. This gives us the opportunity to underline the contribution of cognitive sciences in the analysis of the ontologies of fiction, so as to have these characters perfectly integrate – despite the objections of Sperber & Hirschfeld (2004) – the “cognitive foundations” of our cultures. We defend the idea that superheroes belong to the category of fantastical creatures and we therefore use, for their analysis, the heuristic frame suggested by Armand (2015) and which allows the study of fantastical beings spotted in Folkloristics with the help of Neurocognitive Anthropology. This frame allowed us to analyze the multisensorial powers of superheroes in terms of intuitions, counter-intuitions, and over-intuitions so as to better understand the cognitive mechanisms on the basis of the imaginary of theirs creators and of those who adhere to it.Batman, a vigilante dressed as a bat, uses in his fight against crime the most ambivalent representations of this particular animal in our imaginaries. He remains, however, unable to use in his fiction a natural biological perception of the chripotera, namely animal echolocation. Although it is well known by the public, the functioning of this capacity remains too counter-intuitive to be accurately represented in the stories.Daredevil allows us to continue our study of multisensoriality with the case of a blind vigilante with increased perceptive and attentional capacities. But this “super-blind” is incapable of using the over-intuitive aspect of human echolocation, a skill that seems, in the end, to depend counter-intuitively on the cortical visual areas rather than on the auditory areas (since Thaler et al., 2011).Finally, Superman apparently possesses all powers but he is particularly well known for his ability to fly. This capacity led us to look for the neural incubator of fantastical ontologies in which the narrative found the generative component of this famous wingless flight. It was thus necessary to use out-of-body experiences (or OBE), which have been reported for a long time and are universally recognized in experience narratives and whose emergence has only been recently understood neurologically (see for the instance the experiences of cortical stimulations in Blanke et al., 2002). We offer an approach that would allow the unification of Bachelard’s Training of the Scientific Mind (1938), and which gives the basis of an epistemology of counter-intuition, with his poetics. Indeed, we have been able to redefine the poetics of his Air and Dreams (1943), in its most important contributions – here the wingless dream flight – as over-intuitive. This wingless flight finds a real mode of expression in the movements imagined in virtual worlds and in the experience of body dissociation offered by Virtual Reality.

Made available in DSpace on 2017-05-12T14:47:06Z (GMT). No. of bitstreams: 1 Dissertacao_ Adilson Bosetto 2015 PDF.pdf: 2419034 bytes, checksum: 3fdc9e711199e04fe0746f89b07c4bea (MD5) Previous issue date: 2015-03-10 / The application of enzymes in industrial processes, in its broad sense, has shown the market evolution for innovative alternatives for preserving the environment. Brazil has a great potential to develop some technologies, which allow the use of such materials as substratum for products with higher added value, due to the large amount of lignocellulose as waste that comes from agriculture. Therefore, the analysis of genes expression related to microbial degradation of plant cell wall has caught the researchers attention, mainly because it is associated to the possibility of controlled large-scale synthesis of enzymes applied in biofuel production. In this context, the Gram-negative bacterium C. crescentus is found as a promising microorganism for biotechnological exploitation due to its ability on degrading xylan, the major component of plant hemicellulose. There are several genes in the bacterial genome that codify to Xylanases and β-Xylosidases. In order to purify and biochemically characterize the β-Xylosidase III protein of C. crescentus, xynB3 gene (CCNA_00856) that contains 1,623 nucleotides and encodes a protein with conserved domains of β-Xylosidase with 540 amino acid residues has been studied. Therefore, xynB3 gene was isolated from genomic DNA of C. crescentus NA1000 by Polymerase Chain Reaction (PCR) using specific primers. The single amplification product was cloned into pJet1.2Blunt vector in non-cohesive sites and reintroduced in vector of pTrcHisA expression within the reading frame to produce a histidine tag at the amino-terminus area of fusion protein. The obtained construction was denominated pTrcHis-xynB3 and the confirmation of its gene identification was figured out by the DNA sequence after insertion into the TOP10 E. coli strain and subsequent experimental tests of expression in different temperature of growth, IPTG concentrations and induction times. The recombinant protein was overexpressed into inclusion bodies, thus, in a non-soluble form. Different induction and purification protocols were used to obtain the β-xylosidase III pure of C. crescentus, in native or non-native form. However, assays of enzymatic activity with different substrates neither demonstrated β-xylosidase activity nor detectable levels of the protein. These results suggest that the enzyme was not active during the assays, due its expression in inclusion bodies. This suggests that this protein may have a toxic effect on E. coli when expressed at high levels. Thus, this trial contributes to additional data about the xylanolytic complex concerning the aquatic bacterium C. crescentus. / A utilização de enzimas em processos industriais, no seu sentido mais amplo, demonstra a evolução do mercado em relação a alternativas inovadoras de preservação do meio ambiente. Devido à grande quantidade de material lignocelulósico residuário, proveniente da agricultura, o Brasil é um país com elevado potencial para o desenvolvimento de tecnologias que possibilitem a utilização desses materiais como substrato para produtos de maior valor agregado. Dessa forma, o estudo da expressão de genes microbianos relacionados com a degradação da parede celular vegetal tem despertado a atenção de pesquisadores, principalmente pelo fato de estar relacionado com a possibilidade de síntese controlada e em larga escala de enzimas utilizadas na produção de biocombustíveis. Neste contexto, a bactéria gram-negativa Caulobacter crescentus encontra-se como um microrganismo promissor para a exploração biotecnológica em função da capacidade que tem de degradar o xilano, principal componente hemicelulósico das plantas. Essa bactéria contém em seu genoma vários genes que codificam para xilanases e β-xilosidases. Dentre eles o gene xynB3 (CCNA_00856), que apresenta 1623 nucleotídeos e codifica uma proteína contendo domínios conservados de β-xilosidase, com 540 resíduos de aminoácidos, denominada neste trabalho como β-xilosidase III de C. crescentus. Com o objetivo de possibilitar em estudos futuros a caracterização bioquímica dessa proteína, foi estudado o gene xynB3. Para isso, xynB3 foi isolado a partir do DNA genômico de C. crescentus NA1000 por Reação em Cadeia da Polimerase (PCR) usando oligonucleotídeos específicos. O único produto de amplificação foi clonado no vetor pJet1.2blunt em sítios não coesivos e reintroduzido no vetor de expressão pTrcHisA dentro do quadro de leitura para a produção de uma cauda de histidinas na região amino-terminal da proteína de fusão. A construção obtida foi denominada pTricHis-xynB3 e após confirmação da identidade da mesma por sequenciamento de DNA foi inserida na cepa TOP10 de Escherichia coli e submetida a ensaios experimentais de expressão com diferentes temperaturas de crescimento, concentrações de IPTG e tempos de indução. A proteína recombinante foi super-expressa em corpos de inclusão, portanto, em uma forma não solúvel. Diferentes protocolos de indução e purificação foram empregados para obtenção da β-xilosidase III de C. crescentus pura, em forma nativa ou não nativa. Entretanto, nos ensaios de atividade enzimática, com diferentes substratos, foram obtidos níveis de atividade de β-xilosidase não detectáveis pelas ferramentas utilizadas. Esses resultados sugerem que a enzima não se mostrou ativa durante os ensaios, em função da formação de corpos de inclusão. Isso leva a crer que essa proteína pode apresentar efeito tóxico para E. coli quando expressa em níveis elevados. Assim, este trabalho contribui com dados adicionais a cerca do complexo xilanolítico da bactéria aquática C. crescentus.

This thesis presents new approaches to image registration and super-resolution mosaicing as well as their applications. Firstly, a feature-based image registration method is proposed for a multisensor surveillance system that consists of an optical camera and an infrared camera. By integrating a non-rigid object tracking technique into this method, a novel approach to simultaneous object tracking and multisensor image registration is proposed. Based on the registration and fusion of multisensor information, automatic face detection is greatly improved. Secondly, some extensions of a gradient-based image registration method, called inverse compositional algorithm, are proposed. These extensions include cumulative multi-image registration and the incorporation of illumination change and lens distortion correction. They are incorporated into the framework of the original algorithm in a consistent manner and efficiency can still be achieved for multi-image registration with illumination and lens distortion correction. Thirdly, new super-resolution mosaicing algorithms are proposed for multiple uncompressed and compressed images. Considering the process of image formation, observation models are introduced to describe the relationship between the superresolution mosaic image and the uncompressed and compressed low-resolution images. To improve the performance of super-resolution mosaicing, a wavelet-based image interpolation technique and an approach to adaptive determination of the regularization parameter are presented. For compressed images, a spatial-domain algorithm and a transform-domain algorithm are proposed. All the proposed superresolution mosaicing algorithms are robust against outliers. They can produce superresolution mosaics and reconstructed super-resolution images with improved subjective quality. Finally, new techniques for super-resolution sprite generation and super-resolution sprite coding are proposed. Considering both short-term and long-term motion influences, an object-based image registration method is proposed for handling long image sequences. In order to remove the influence of outliers, a robust technique for super-resolution sprite generation is presented. This technique produces sprite images and reconstructed super-resolution images with high visual quality. Moreover, it provides better reconstructed low-resolution images compared with low-resolution sprite generation techniques. Due to the advantages of the super-resolution sprite, a super-resolution sprite coding technique is also proposed. It achieves high coding efficiency especially at a low bit-rate and produces both decoded low-resolution and super-resolution images with improved subjective quality. Throughout this work, the performance of all the proposed algorithms is evaluated using both synthetic and real image sequences.

Depuis la réalisation expérimentale en 1995 des premiers condensats gazeux de<br />Bose-Einstein (B-E) d'atomes alcalins : $\left(<br />^(87)Rb\,\ , \ ^(23)Na\,\ , \ ^(7)Li\right) $, ultra-froids ($T=0\,^(\mathrm(o))\!\mathrm(K)$) et confinés dans des pièges magnétiques 3D, la physique des condensats de Bose-Einstein et<br />de Fermi a connu un développement remarquable aussi bien expérimental que<br />théorique. L'objectif de ce mémoire de thèse a été fixé dans le cadre général du progrès récemment accompli dans l'étude de l'évolution dynamique des condensats<br />de B-E répulsifs, et de la réduction de leur dimensionnalité. Le manuscrit de<br />cette thèse comprend deux parties. La première a été consacrée, d'une part, à la<br />présentation du phénomène de la condensation de B-E depuis sa prédiction en 1925 par Einstein, dans un gaz idéal de Bose, jusqu'à sa réalisation en 1995, et<br />d'autre part, à la description de la dynamique des condensats dilués de B-E, à<br />la température $T=0\,^(\mathrm(o))\!\mathrm(K)$, par l'équation nonlinaire de Schr\"(o)dinger (ENLS), connue aussi sous le nom : équation de Gross-Pitaevskii (EGP). La seconde partie<br />comprend les résultats numériques de notre étude portant sur la dynamique d'un<br />condensat de B-E répulsif, quasi-1D et confiné dans un piège non-harmonique<br />(piège allongé avec des bords paraboliques), et sur son comportement dissipatif<br />et superfluide. Notre étude a montré que: i) les bords paraboliques du piège<br />considéré, ainsi qu'un obstacle en forme d'une bosse gaussienne, placé dans la partie plate<br />de ce piège, ont un effet d'anti-amortissement sur la propagation uniforme d'un<br />soliton gris dans le condensat, et cet effet se manifeste par une émission spontanée des<br />phonons; ii) le mouvement uniforme et rectiligne (en va-et-vient) d'un obstacle gaussien dans le condensat considéré conduit, lorsque la vitesse constante de l'obstacle<br />dépasse une certaine valeur critique ( vitesse critique ), à la création des solitons gris et des phonons dans ce<br />condensat qui devient un milieu dissipatif.<br /> Nous avons montré que le comportement dissipatif du condensat croît avec l'augmentation de la vitesse de<br />l'obstacle, atteint son maximum et finit par disparaître quasi-totalement pour<br />de grandes valeurs de la vitesse constante de l'obstacle, pour lesquelles le condensat se comporte comme un quasi-superfluide.