Economic viability of Fraser River, Labrador Arctic charr (Salvelinus alpinus) aquaculture in Atlantic Canada may be greatly improved if grow-out could be completed in seawater (30 ppt), while having a low incidence of sexual maturation before harvesting. Growth and survival in seawater was investigated among individually PIT-tagged Arctic charr reared in tanks in the laboratory. Direct transfer from freshwater to brackish water (20 ppt), and then acclimation to 30 ppt was successful. The manipulation of photoperiod, temperature, and food ration can be used as practical applications in aquaculture to arrest maturation; this was investigated in two additional experiments. The most effective photoperiod was LD18:6 for 6 weeks starting December 21, which reduced maturation to 43% compared to 78% in controls. Restricted ration from December 21 through March 15 had no effect on maturation, however, rearing females in 5°C compared to 10°C reduced maturation to 15% compared to >80% in controls.

No description available.

To determine the level of salt tolerance of the medicinal plant Echinacea, the physiological and biochemical characteristics of E. purpurea, E. pallida and E. angustifolia exposed to different NaCl levels (0, 50, 75, and 100 mM) were evaluated under hydroponic culture. Dry weights of shoots and roots were not affected by salinity; however E. purpurea and E. pallida exhibited higher survival rate than E. angustifolia, which also showed high salt injury index and electrolyte leakage compared to the other two species. Gas exchange (photosynthetic rate, stomatal conductance, and transpiration rate) showed a decline with increasing salt concentrations in all species with a more pronounced reduction in E. angustifolia. E. purpurea was able to retain more Na+ in the roots than the other two species showing its capacity to regulate Na+ translocation to shoots (Na+ exclusion). Moreover, the activities of two major antioxidant enzymes; superoxide dismutase (SOD) and ascorbate peroxidase (APX) were increased by salinity in E purperea, while the activities were decreased in E. angustifolia. The characteristic phytochemical profiles of caffeic acid derivatives (CADs) and alkamides/ketones were obtained for the three species, and quantitative changes were determined. Cichoric acid, the major CAD in E. purpurea, was increased with salinity up to 75 mM NaCl. A relative increase in alkamides and CADs was recorded in E. angustifolia, while in E. pallida, the level of echinacoside and major ketones (22 and 24) decreased, suggesting that the medicinal value of this species was compromised by salt stress. First evidence of salt-induced changes in alkamides and ketones in Echinacea was demonstrated in this study. Activity of phenylalanine ammonia-lyase (PAL), the major enzyme involved in the biosynthesis of CADs, was increased only in the roots of E. purpurea, further reflecting the differences in salt tolerance between species. It can be concluded that Echinacea species exhibited a limited degree of salt tolerance; however, E. purpurea showed a higher tolerance than E. pallida and E. angustifolia. This tolerance was mainly attributed to the increase in Na+ exclusion capacity, antioxidant activities and PAL activity.

Lake Ellesmere/Te Waihora is one of New Zealand’s largest coastal, brackish water lakes. It has nationally significant wetland bird populations and is regionally important for iwi. The lake regularly experiences fluctuations in water level, resulting in a continually expanding and contracting littoral zone. This study investigated the impacts of these water level changes on the ecology of the lake. Water chemistry results collected over 12 months, confirm the lake is hypertrophic, due to high nutrient (nitrogen and phosphorus) concentrations resulting in high chlorophyll a levels and low water clarity. Water chemistry conditions were collected at five locations around the lake and showed marked spatial variation, with the eastern most end (Kaituna Lagoon) having generally the best water quality and lowest salinity (mean 4.9 ppt). Mean concentrations of total nitrogen ranged from 1.63 to 2.4 mg/L, chlorophyll a from 50 to 148 ug/L and total suspended solids from 151 – 248 mg/L. Seasonally, highest nutrient concentrations (mean, total nitrogen = 2.625 mg/L, dissolved reactive phosphorus = 0.059 mg/L and total phosphorus = 0.365 mg/L) occurred in late summer months (February – March), slightly decreasing but remaining high throughout winter. The benthic invertebrate community was surprisingly diverse, Crustacea (Paracorophium excavatum), Oligochaeta, Mollusca (Potamopyrgus antipodarum) and Chironomidae (Chironomus zealandicus) were dominant community members in the littoral zone, although 24 other taxa were collected. At high water levels, taxonomic richness increased in the eulittoral zone, while decreasing in the mid-littoral and lower littoral zones. In contrast, density decreased with higher water level in the eulittoral and mid-littoral zones, while increasing in the lower littoral zone. Benthic invertebrate communities appeared to be adapted to periods of intermittent dewatering, and even sustained dewatering under cooler temperatures. Despite the relatively high diversity of benthic invertebrates, invertebrate predators are generally absent from the lake. My results suggest multiple factors and interactions from predation pressure, salinity and lack of macrophytes are likely responsible for the absence of predatory invertebrates such as damselfly (Xanthocnemis zealandica) and dragonfly (Procordulia grayi) larvae. The lack of significant relationships between water quality variables and water level, and the positive relationship between chlorophyll a and salinity, suggests that current lake opening events do not have a positive effective on either water quality or phytoplankton biomass in Lake Ellesmere/Te Waihora. However, the current lake opening regime seems to be favourable to benthic invertebrate survival in the littoral zone, as the lake is predominantly open over winter when temperatures are lower, reducing the risk of desiccation. Anthropogenic activities which modify hydrodynamic and water quality conditions can potentially have a large negative impact on the structure and diversity of the littoral invertebrate community as well as flow on effects through the lake food web. Based on results from this study, I suggest a minimum lake level at Taumutu of 0.6 m during the months from November – April in order to protect benthic invertebrate communities in the eulittoral zone from extensive loss of habitat, extreme temperature and reduced risk of desiccation. Having a minimum set at ~0.6 m would provide sufficient littoral zone habitat for the lakes extensive bird life and fish populations. In addition, immediate efforts are needed into reducing nutrient loads into the lake, through improved farm management (nutrient and stocking budgets) and riparian fencing. Furthermore, physical and chemical water quality properties would benefit from an increased water level over summer months, by reducing water temperatures, diluting readily available nutrient concentrations and potentially reducing phytoplankton (and potentially toxic cyanobacterial) blooms.

To determine the level of salt tolerance of the medicinal plant Echinacea, the physiological and biochemical characteristics of E. purpurea, E. pallida and E. angustifolia exposed to different NaCl levels (0, 50, 75, and 100 mM) were evaluated under hydroponic culture. Dry weights of shoots and roots were not affected by salinity; however E. purpurea and E. pallida exhibited higher survival rate than E. angustifolia, which also showed high salt injury index and electrolyte leakage compared to the other two species. Gas exchange (photosynthetic rate, stomatal conductance, and transpiration rate) showed a decline with increasing salt concentrations in all species with a more pronounced reduction in E. angustifolia. E. purpurea was able to retain more Na+ in the roots than the other two species showing its capacity to regulate Na+ translocation to shoots (Na+ exclusion). Moreover, the activities of two major antioxidant enzymes; superoxide dismutase (SOD) and ascorbate peroxidase (APX) were increased by salinity in E purperea, while the activities were decreased in E. angustifolia. The characteristic phytochemical profiles of caffeic acid derivatives (CADs) and alkamides/ketones were obtained for the three species, and quantitative changes were determined. Cichoric acid, the major CAD in E. purpurea, was increased with salinity up to 75 mM NaCl. A relative increase in alkamides and CADs was recorded in E. angustifolia, while in E. pallida, the level of echinacoside and major ketones (22 and 24) decreased, suggesting that the medicinal value of this species was compromised by salt stress. First evidence of salt-induced changes in alkamides and ketones in Echinacea was demonstrated in this study. Activity of phenylalanine ammonia-lyase (PAL), the major enzyme involved in the biosynthesis of CADs, was increased only in the roots of E. purpurea, further reflecting the differences in salt tolerance between species. It can be concluded that Echinacea species exhibited a limited degree of salt tolerance; however, E. purpurea showed a higher tolerance than E. pallida and E. angustifolia. This tolerance was mainly attributed to the increase in Na+ exclusion capacity, antioxidant activities and PAL activity.

Injection of low-salinity water showed high potentials in improving oil recovery when compared to high-salinity water. However, the optimum water salinity and conditions are uncertain, due to the lack of understanding the mechanisms of fluid-rock interactions. The main objective of this study is to examine the potential and efficiency of low-salinity water in secondary and tertiary oil recovery for sandstone reservoirs. Similarly, this study aims to help in understanding the dominant mechanisms that aid in improving oil recovery by low-salinity waterflooding. Furthermore, the impact of cation type in injected brines on oil recovery was investigated. Coreflood experiments were conducted to determine the effect of water salinity and chemistry on oil recovery in the secondary and tertiary modes. The contact angle technique was used to study the impact of water salinity and composition on rock wettability. Moreover, the zeta potential at oil/brine and brine/rock interfaces was measured to explain the mechanism causing rock wettability alteration and improving oil recovery. Deionized water and different brines (from 500 to 174,000 mg/l), as well as single cation solutions were tested. Two types of crude oil with different properties and composition were used. Berea sandstone cores were utilized in the coreflood experiments. Coreflood tests indicated that injection of deionized water in the secondary mode resulted in significant oil recovery, up to 22% improvement, compared to seawater flooding. However, no more oil was recovered in the tertiary mode. In addition, injection of NaCl solution increased the oil recovery compared to injection of CaCl2 or MgCl2 at the same concentration. Contact angle results demonstrated that low-salinity water has an impact on the rock wettability; the more reduction in water salinity, the more a water-wet rock surface is produced. In addition, NaCl solutions made the rock more water-wet compared to CaCl2 or MgCl2 at the same concentration. Low-salinity water and NaCl solutions showed a highly negative charge at rock/brine and oil/brine interfaces by zeta potential measurements, which results in greater repulsive forces between the oil and rock surface. This leads to double-layer expansion and water-wet systems. These results demonstrate that the double-layer expansion is a primary mechanism of improving oil recovery when water chemical composition is manipulated.

Salt affected soils and their effects on land and water resources have been identified as one of the most severe environmental problems facing Australia. This current study focused on the incorporation of recycled organic products (RO) into an acidic saline soil that had been irrigated with an industrial effluent (IE), specifically to investigate the potential for these organics to be used in rehabilitation. Compost incorporated into the acidic saline soil was able to raise pH to more favourable levels required for plant growth (pH 6 – 7.5). Plant growth was however dependent on the input material of the compost as well as the irrigation scheme. The soils amended with this compost generally showed higher and more rapid microbial activity, measured by CO2 emissions, in all amendment rates than the plant derived compost. Overall it could be concluded that the application of RO on saline soils improved the establishment and growth of plants and alleviated to some degree the negative effects of IE. However great care should be taken at the selection of the input material, as high rates of ammonium, calcium and other soluble salts can increase the EC of an amended soil further.

Soil salinity (due to ingress of excess amounts of dissolved salts in soil pores) and soil sodicity (due to excess amounts of sodium ions attached to the clay surface) are significant forms of land degradation in many parts of the world in particular in arid and semi arid regions. In Australia, soil salinity has long been identified as the major form of land degradation and the greatest environmental threat. Saline soils cover almost 6% of Australia’s land mass and impose severe threats on agricultural productivity and built infrastructure with an estimated annual loss of $250 million. In recent years, ‘soil sodicity’ is recognised as a far more significant form of land degradation and a severe environmental problem both in terms of affected land area and impact on the environment than is salinity as a problem in Australia. One third of Australian land mass is occupied by sodic soils costing an estimated $2 billion each year in lost production alone, with further significant impacts on the economy due to extensive damage to infrastructure facilities and the environment. [...] / Doctor of Philosophy

At no point in history have we demanded so much from our agricultural land whilst simultaneously leaving so little room for management error. Of the many possible environmental impacts from agriculture, soil and water salinisation has some of the most long-lived and deleterious effects. Despite its importance, however, land managers are often unable to make informed decisions of how to manage the risk of salinisation due to a lack of data. Furthermore, there remains no universally agreed method for salinity risk mapping. This thesis addresses these issues by investigating new methods for producing high-resolution predictions of soil salinity, soil physical properties and groundwater depth using a variety of traditional and emerging ancillary data sources. The results show that the methodologies produce accurate predictions yielding natural resource information at a scale and resolution not previously possible. Further to this, a new methodology using fuzzy logic is developed that exploits this information to produce high-resolution salinity risk maps designed to aid both agricultural and natural resource management decisions. The methodology developed represents a new and effective way of presenting salinity risk and has numerous advantages over conventional risk models. The incorporation of fuzzy logic provides a meaningful continuum of salinity risk and allows for the incorporation of uncertainty. The method also allows salinity risk to be calculated relative to any vegetation community and shows where the risk is coming from (root-zone or groundwater) allowing more appropriate management decisions to be made. The development of this methodology takes us a step closer to closing what some have called our greatest gap in agricultural knowledge. That is, our ability to manage the salinity risk at the subcatchment scale.

Spicers Creek catchment is located approximately 400 km west of Sydney in the Central West region of New South Wales, Australia. Dryland salinity has been recognised as a major environmental issue impacting soil and water resources in the Central West region of NSW for over 70 years. Due to the geological complexity of the catchment and the presence of high salt loads contained within the soils, groundwater and surface waters, the Spicers Creek catchment was identified as a large contributor of salinity to the Macquarie River catchment. Over fifty-two dryland salinity occurrences have been identified in the Spicers Creek catchment and it appears that dryland salinity is controlled by the presence of geological structures and permeability contrasts in the shallow aquifer system. Combinations of climatic, geological and agricultural factors are escalating salinity problems in the catchment. The main aim of this thesis was to identify the factors affecting salinisation processes in the Spicers Creek catchment. These include the role of geological structures, the source(s) of salts to the groundwater system and the geochemical processes influencing seepage zone development. To achieve these aims a multidisciplinary approach was untaken to understand the soils, geology, hydrogeology and hydrogeochemistry of the catchment. Investigative techniques employed in this project include the use of geophysics, soil chemistry, soil spectroscopy, hydrogeochemistry and environmental isotopes. Evaluation of high-resolution airborne magnetics data showed a major north-east to south-west trending shear zone. This structure dissects the catchment and several other minor faults were observed to be splays off this major structure. These structures were found to be conducive to groundwater flow and are influencing the groundwater chemistry in the fractured aquifer system. Two distinctive groundwater chemical types were identified in the catchment; the saline Na(Mg)-Cl-rich groundwaters associated with the fractured Oakdale Formation and the Na-HCO3-rich groundwaters associated with the intermediate groundwater system. The groundwater chemistry of other deep groundwaters in the catchment appears to be due to mixing between these end-member groundwaters within the fractured bedrock system. The spatial distribution of electrical conductivity, Cl-, Sr2+ and 87Sr/86Sr isotopic ratios showed the correlation between saline groundwaters and the location of faults. Elevated salinities were associated with the location of two crosscutting fault zones. The spatial distribution of HCO3-, K+, Li+ and ?????3CDIC highlighted the extent of Na-HCO3-rich groundwaters in the catchment and showed that these groundwaters are mixing further east than previously envisaged. These findings show that Na(Mg)-Cl-rich groundwaters are geochemically distinctive and have evolved due to extensive water-rock interaction processes within the fracture zones of the Oakdale Formation. These saline groundwaters contain elevated concentrations of trace elements such as As, V and Se, which pose a potential risk for water resources in the area. 87Sr/86Sr isotopic ratios indicated that the source of salinity to the Na(Mg)-Cl-rich groundwaters was not purely from marine or aerosol input. Salt is most likely contributed from various allochthonous and autochthonous sources. This research found that the main mechanism controlling the formation of dryland salinity seepage zones in the Spicers Creek catchment is due to the presence of geological structures. These groundwater seepage zones act as mixing zones for rainfall recharge and deeper groundwaters. The main sources of salt to the seepage zones are from deeper Na(Mg)-Cl-rich groundwaters and rainfall accession. The major importance of this research highlights the need for an integrated approach for the use of various geoscientific techniques in dryland salinity research within geologically complex environments.