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The impact of the term of defoliation of the zone of grape on quality and condition of plant health for the Sauvignon blanc variety The focus of the Thesis is to evaluate the effect of the term of defoliation of the zone of grape on quality and condition of plant health for Sauvignon blanc variety. The introduction of the theoretical part briefly characterizes the issue of leaf area -- factors affecting it. The next sections describe the green work and the attention is focused primarily on defoliation of the zone of grape -- the impact, the terms and the intensity of defoliation. The factors determining the ripeness and quality of grapes are briefly described as well individual qualitative parameters. The practical section describes he methodology used in the evaluation of the experiment of defoliation on four different terms and the results of the examined variants of defoliation are interpreted.

The purpose of the thesis on topic Effect of date of defoliation on aromatic profile ´Sauvignon blanc´ wine was to find out the effect of removing leaves from grapes zone on qualitative and quantitative parameter of grapes of this species. The practical part of the experiment with defoliation in different terms was carried out during 2015 on a vineyard in Svatobořice-Mistřín. Part of the practical experiment was the production of wines from different variants and and evaluated the sensory properties of wine.

This diploma thesis compares the differences between clones of the Sauvignon variety, which are planted in the Central Slovakia wine region in the village of Veľký Krtíš and then evaluates the results of this comparison. Three clones from French and one clone from Tyrol are planted together in one vineyard track. The literary part of this thesis describes general information about Sauvignon variety, its clones and aromatic substances. In the vineyard were studied the phenological phases of the clones, uvological values and yield. From each clone has been made wine separately. For each wine were determined basic parameters and difference of aromatic substances using method of comprehensive two-dimensional gas chromatography. Based on the results of the expert committee were created the aromagrams and the position of the wines in the hundred-point table according to standards of the O.I.V.

The thesis deals with the issue of defoliation after quality grapes. The work deals with the comparison of mechanical and manual defoliation. The first part deals with searching and displaying information with this issue. The second part is about my experiment, its processing and its own recommendations. I was able to do this experiment on the Plaček vineyard in Moravské Branice where I used 11 lines of Sauvignon blanc variety for the experiment when every line indicates another experiment variation. Defoliation zone of grapes took place since 7.6.2018. Manual defoliation always removed 1-3 leafs and other part from defoliation zone. Since 15.6.2018 we used a mechanized defoliation using the defoliator. The following experimental variations were made in the same terms both manually and mechanically. The last mechanical defoliation was on 1.7.2018 and we performed a defoliation variant as a control. The last attempts were made between 26.7 and 2.8.2018 where we used another method of defoliation zone of grapes for a purpose to slown down the ripening. During the ripening of the grapes their health and quality was monitored. In four weeks carried out the evaluation of qualitative grapes. The berry sugar content, Ph value, acid content, nitrogen content and other defoliation-related parameters were measured. All measurements were taking place at a school laboratory in Lednice. All data have been evaluated and we written own recommendations for winemakers and vineyard oweners.

Thesis (PhD(Agric))--Stellenbosch University, 2014. / ENGLISH ABSTRACT: This study focussed on the effect of oxygen on the chemical and sensory evolution of Sauvignon blanc wine under controlled oxidation conditions. The sensorial interactive effects between Sauvignon blanc varietal aroma compounds and compounds that typically arise during oxidation of white wines were also investigated. In the first research chapter the sensorial interactive effects of Sauvignon blanc impact compounds with aldehydes typically originating from oxidation of white wines were investigated. Four compounds, 3‐ mercaptohexan‐1‐ol (3MH), 3‐isobutyl‐2‐methoxypyrazine (IBMP), 3‐(methylthio)‐propionaldehyde (methional) and phenylacetaldehyde were added together in a model wine medium at varying concentrations. The concentrations chosen were according to those reported in literature to occur in Sauvignon blanc wines. The sensory effects of these compounds were profiled using a trained sensory panel. Compounds were first profiled individually and results showed that the change in comcpound concentration not only led to a change in intensity ratings but also in some cases a change in the descriptor. All four compounds in the same sample showed complicated interactive effects. Data were statistically analysed using relatively novel techniques such as statistical networks that allowed deeper insights into the interactions involved. Various observations were made such as the contribution of 3MH to the ‘green’ character of the wine, the potent suppressing abilities of methional on 3MH and IBMP and the additive effect of methional and IBMP contributing to the ‘cooked’ character of the sample. In the second research chapter the effect of repetitive oxidation on a fresh and fruity style Sauvignon blanc wine was investigated. Results showed the progress and evolution of aromatic and non‐aromatic compounds during an oxidative aging period. A large range of chemical analyses were conducted together with extensive sensory profiling. Results showed a decrease in volatile thiols responsible for the fruity nuances and an increase in oxidation‐related compounds, such as acetaldehyde, during the course of the oxidation. Sensory profiling showed the evolution of the wine aroma during oxidation. The wine evolved from a fresh and fruity wine to slight oxidation and then developed extreme oxidative characteristics. The Control samples (no oxygen added) developed a ‘cooked’ character which could indicate the formation of reductive compounds in these wines. Conversely, the wines that received a single dose of oxygen did not develop this flavour and were perceived to be more fresh and fruity than the Control samples. The evolution of the wine colour was also monitored using a spectrophotometer as well as a sensory panel and results suggest that the colour of the wine evolved before the disappearance of the pleasant aroma. The advantages and disadvantages of oxygen exposure to this type of wine style are discussed as well as the complexity of the wine matrix and sensory interactions occurring in the specific wine. The aim of the third research chapter was again to investigate further sensory interactions between Sauvignon blanc varietal aroma compounds and an oxidation‐related compound. After the observations reported in the second research chapter, the inclusion of acetaldehyde in an interaction study seemed to be of critical importance. Acetaldehyde can reach significant concentrations during oxidation and can have a detrimental effect on wine aroma. This interaction study included three compounds, 3MH, IBMP and acetaldehyde. Acetaldehyde was able to effectively mask the ‘green’ character of the sample, while it also enhanced certain fruity nuances when present at specific concentrations. 3MH was able to suppress the oxidising character of acetaldehyde when present at sufficient concentrations. The results from this study clearly showed the complexity of the wine medium and the interactions involved. It also highlighted the importance of performing these types of sensory studies in a simple medium opposed to a complex matrix such as wine. The effect of oxygen on various aspects of the wine was investigated and the combination of chemical and sensory data delivered some interesting conclusions also involving interactions that occurred. This study paved the way for future investigations on the sensory relationships of Sauvignon blanc aroma compounds and the role of proper oxygen management in the production of quality wines. / AFRIKAANSE OPSOMMING: Die doelwit van die studie was om die effek van gekontroleerde oksidasie op die ontwikkeling en verloop van verskeie chemiese komponente asook die ontwikkeling van die sensoriese profiel van ‘n Sauvignon blanc wyn te ondersoek. Bykomende studies ondersoek die interaksie/wisselwerking tussen aromakomponente in ‘n eenvoudige matriks. Interaksies tussen spesifieke Sauvignon blanc kultivargeassosieerde aromakomponente en komponente wat normaalweg tydens die oksidasie van witwyne ontwikkel, word ondersoek. Die eerste navorsingshoofstuk het ten doel om die interaksie tussen tipiese Sauvignon blanc aromakomponente (of impakkomponente) met aldehiede wat normaalweg tydens oksidasie van witwyne ontwikkel, na te vors. Vier komponente is in ‘n modelwyn gevoeg teen verskeie konsentrasies, wat oor die algemeen in die literatuur gerapporteer is om voor the kom in Sauvignon blanc wyne. Die komponente wat ondersoek is, is: 3‐merkaptoheksanol (3MH), 3‐isobutiel‐2‐metoksiepirasien (IBMP), 3‐ (metieltio)‐propionaldehied (methional) en fenielasetaldehied. Die sensoriese effekte van die komponente is deur ‘n opgeleide sensoriese paneel geëvalueer. Komponente is eers individueel geanaliseer en die resultate het getoon dat dat die intensiteit van die spesifieke aroma verander namate die konsentrasie verander. In sommige gevalle het die beskrywende woord vir die aroma ook verander. Deur al vier komponente in dieselfde monster te voeg word die ondersoek gekompliseer. Die data is statisties geanaliseer deur gebruik te maak van relatiewe nuwe tegnieke soos statistiese netwerke wat dieper insig in die betrokke interaksies bewerkstellig. Verskeie waarnemings word gerapporteer onder andere die bydrae van 3MH tot die ‘groen’ karakter van die wyn, die kragtige onderdrukkingsvermoë van methional op 3MH en IBMP asook die opbouende effek van methional en IBMP wat bydra tot die ‘gekookte’ karakter van die monster. Die tweede navorsingshoofstuk is daarop gemik om die effek van herhalende oksidasie op 'n vars en vrugtige styl Sauvignon blanc wyn te ondersoek en om die vordering en ontwikkeling van aromatiese en nie‐aromatiese komponente gedurende hierdie tydperk te analiseer. ‘n Wye reeks chemiese komponente is geanaliseer tesame met omvangryke sensoriese analise. ‘n Afname in die vlugtige tiole, wat verantwoordelik is vir die vrugtige geure, is gevind tesame met ‘n toename in oksidasie‐verwante komponente (soos asetaldehied). Sensoriese ondersoeke toon ook die evolusie van die wynaroma tydens oksidasie. Die wyn het ontwikkel van ‘n vars en vrugtige styl na effense oksidasiegeure waarna ekstreme oksidasiekarakters waargeneem is. Die Kontrole monsters het ‘n ‘gekookte’ karakter ontwikkel wat ‘n aanduiding van die ontwikkeling van ‘reduktiewe’ komponente in hierdie wyn kan wees. Aan die ander kant het wyne wat een suurstofdosering ontvang het, geen van hierdie geure ontwikkel nie en die wyn is as varser en vrugtiger beskryf in vergelyking met die Kontrole monsters. Die ontwikkeling van die wynkleur is ook gemonitor deur gebruik te maak van ‘n spektrofotometer asook 'n sensoriese paneel. Resultate stel voor dat die kleur van die wyn ontwikkel voor die aangename geure begin verdwyn. Die voor‐ en nadele van suurstofblootstelling aan hierdie tipe wynstyl word bespreek asook die kompleksiteit van die wynmatriks en sensoriese interaksies wat in hierdie spesifieke wyn voorkom. Die derde navorsingshoofstuk is weereens daarop gemik om die sensoriese interaksie tussen tipiese Sauvignon blanc kultivar‐geassosieerde aromakomponente en nog ‘n oksidasie‐geassosierde aromakomponent te ondersoek. Die resultate vanuit die tweede navorsingshoofstuk het die insluiting van asetaldehied in die interaksiestudie genoodsaak. Asetaldehied kan betekenisvolle konsentrasies tydens oksidasie behaal en kan ook nadelige effekte op wynaroma hê. Hierdie interaksiestudie het die volgende drie komponente ingesluit: 3MH, IBMP en asetaldehied. Asetaldehied het die ‘groen’ karakter van IBMP effektief gemaskeer terwyl dit die waarneming van die vrugtige aroma ondersteun en selfs verhoog het wanneer dit teen sekere konsentrasies teenwoordig was. 3‐Merkaptoheksanol het die oksidasiekarakter van asetaldehied onderdruk wanneer dit teen genoegsame konsentrasies teenwoordig was. Die kompleksiteit van wyn as ‘n navorsingsmedium is duidelik vanuit die studie veral in die ondersoeking van interaksie‐effekte tussen komponente. Die belangrikheid van die gebruik van 'n eenvoudige medium teenoor ‘n komplekse medium vir soortgelyke studies is dus duidelik. Die effek van suurstof op verskeie aspekte van witwyn is ondersoek en die kombinasie van chemiese en sensoriese data het interessante gevolgtrekkings gelewer. Die studie het die pad vir toekomstige studies gebaan in terme van sensoriese interaksies met betrekking tot Sauvignon blanc aroma. Die belangrikheid van oordeelkundige suurstofbestuur tydens die produksie van kwaliteit wyne is ook uitgelig.

Thesis (PhD--Biological Sciences)--University of Auckland, 2010. / Includes bibliographical references.

Thesis (M. Appl. Sc.) -- Lincoln University, 2009. / Also available via the World Wide Web.

Machine harvesting is the most widely expanded vineyard mechanization practices today. Foreign research into the effects of mechanization demonstrated that the grape quality was unaffected, in some cases, improved with the introduction of machine harvesting. In 2015 within the framework of this diploma work the experiment was performed by Sauvignon blanc. All data were subjected to Analysis of variance at P < 0,05. Although statistical differences were found in the grapes by total acidity and type of harvesting, statistical differences were not found in wine. Wine produced from machine grape harvesting were more fruity in sensory analysis.

Thesis (MScAgric)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Sauvignon blanc wines have become progressively more important in the commercial market. Extensive research is being done in various countries to gain more understanding about the aroma compounds found in Sauvignon blanc wines and the interactions between them. Sauvignon blanc wines often have either have a green or tropical style. The green style is caused by the methoxypyrazines while the volatile thiols are important contributing compounds to the tropical style. Various international studies have focussed on measuring the chemical composition of Sauvignon blanc wines. However, more research is required on South African Sauvignon blanc wines. Little is known of the volatile thiols content of South African Sauvignon blanc wines, although the methoxypyrazine content has been extensively reported on. Although methoxypyrazines and volatile thiols are seen as the most important aroma compounds contributing to Sauvignon blanc character, other compounds contribute as well. Esters, monoterpenes and phenols have been found to influence Sauvignon blanc aroma and interact with the methoxypyrazines and volatile thiols. The complex interaction between the compounds responsible for the aroma of Sauvignon blanc wines are still not fully understood and further research is thus needed. The first part of the current study investigated the interaction between a specific methoxypyrazine and volatile thiol. Five different concentrations of 2-isobutyl-3- methoxypyrazine (ibMP) and 3-mercaptohexan-1-ol (3MH) were spiked in dearomatize, neutral Sauvignon blanc wine. The single compounds as well as every possible combination of the range of concentrations were evaluated using sensory descriptive analysis. It was found using various statistical approaches that ibMP suppressed the tropical attributes associated with 3MH and that 3MH suppressed the green attributes that correlated with ibMP. The concentrations at which the suppression occurred and the degree of suppression was different for each attribute. The second part of the current study focussed on commercial South African Sauvignon blanc wines. Sensory descriptive analysis and chemical analysis were used to assess the wines and measure the volatile thiol and methoxypyrazine concentrations. The concentrations of volatile thiols and methoxypyrazines were found to be in line with international Sauvignon blanc wines. It was also shown for the first time that the mutually suppressive trend between the volatile thiols and methoxypyrazines can be seen in commercial Sauvignon blanc wines as well. Future work is needed to fully understand the complex interaction between the various compounds in Sauvignon blanc wines. Further research could focus on investigating the mechanism of interaction between the volatile thiols and methoxypyrazines as well as other aroma compounds. / AFRIKAANSE OPSOMMING: Sauvignon blanc-wyne word toenemend belangriker in die kommersiële mark. Omvattende navorsing word in etlike lande gedoen om meer begrip te ontwikkel van die aromaverbindings wat in Sauvignon blanc-wyne teenwoordig is, asook van die interaksies tussen hulle. Sauvignon blanc-wyne het in baie gevalle óf ’n groen óf ’n tropiese styl. Die groen styl word veroorsaak deur metoksipirasiene, terwyl die vlugtige tiole belangrike bydraende verbindings is wat aanleiding gee tot die tropiese style. Verskeie internasionale studies het reeds gefokus op die meet van die chemiese samestelling van Sauvignon blanc-wyne, maar meer navorsing is nodig oor Suid-Afrikaanse Sauvignon blanc-wyne. Min is bekend oor die inhoud van vlugtige tiole in Suid-Afrikaanse Sauvignon blanc-wyne, hoewel daar reeds op groot skaal oor die metoksipirasieninhoud verslag gedoen is. Hoewel metoksipirasiene en vlugtige tiole die belangrikste aromaverbindings is wat tot Sauvignon blanc-karakter bydra, is daar ook ander verbindings wat ’n bydrae maak. Esters, monoterpene en fenole het almal ’n invloed op Sauvignon blanc-aroma en reageer op die metoksipirasiene en vlugtige tiole. Die komplekse interaksie tussen die verbindings wat vir die aroma van Sauvignon blanc-wyne verantwoordelik is, word nog nie volledig begryp nie en verdere navorsing is nodig. Die eerste deel van die huidige studie het die interaksie tussen ’n spesifieke metoksipirasien en vlugtige tiol ondersoek. Vyf verskillende konsentrasies van 2-isobutiel-3-metoksipirasien (ibMP) en 3-merkaptoheksaan- 1-ool (3MH) is by ontgeurde, neutrale Sauvignon blanc-wyn gevoeg. Die enkel verbindings, asook elke moontlike kombinasie van die reeks konsentrasies, is deur middel van beskrywende sensoriese analise geëvalueer. Daar is met behulp van verskillende statistiese benaderings gevind dat ibMP die tropiese eienskappe wat verband hou met 3MH onderdruk het, terwyl 3MH die groen eienskappe wat verband hou met ibMP onderdruk het. Die konsentrasies waarteen die onderdrukking plaasgevind het en die vlak van onderdrukking het vir elke eienskap verskil. Die tweede deel van die studie het gefokus op kommersiële Suid-Afrikaanse Sauvignon blancwyne. Beskrywende sensoriese analise en chemiese analise is gebruik om die wyne te assesseer en die konsentrasies van vlugtige tiole en metoksipirasiene te meet. Die konsentrasies vlugtige tiole en metoksipirasiene was in lyn met dié van internasionale Sauvignon blanc-wyne. Daar is ook vir die eerste keer gewys dat die wedersyds onderdrukkende tendens tussen die vlugtige tiole en metoksipirasiene ook in kommersiële Sauvignon blanc-wyne gevind word. Toekomstige werk sou kon fokus op ’n begrip van die komplekse interaksie tussen die verskillende verbindings in Sauvignon blanc-wyne. Verdere navorsing sou ook kon fokus op ’n ondersoek van die meganisme van interaksie tussen die vlugtige tiole en metoksipirasiene, sowel as ander aromaverbindings. / The South African Wine Industry (Winetech), THRIP and the NRF for financial support