Thesis advisor: Peter Weiler / Between 1866 and 1918, suffragists in Britain campaigned to acquire the vote for women. Opposition to women's suffrage derived mainly from separate spheres ideology – the belief that the genders are inherently different and must fulfill different roles in society. Many scholars claim that the suffragists challenged separate spheres ideology. By comparing the writings of Millicent Fawcett and Frances Cobbe, two prominent suffragists, with the writings of Mary Ward and Violet Markham, two prominent anti-suffragists, this work demonstrates similar themes within the opposing campaigns. More importantly, the similarities indicate that suffragists argued within the context of separate spheres ideology and did not seek to significantly alter traditional gender roles. / Thesis (BA) — Boston College, 2005. / Submitted to: Boston College. College of Arts and Sciences. / Discipline: History. / Discipline: College Honors Program.

Les structures de sphères creuses appartiennent à la famille des matériaux cellulaires qui ont récemment été étudiés pour leurs multiples propriétés. Dans le cas de cette thèse, le but des sphères creuses est de dissiper l’énergie d’impact d’un oiseau sur un cockpit d’avion. Elles sont développées dans le cadre du projet SAMBA (Shock Absorber Material for Birdshield Application) afin d’optimiser leur énergie spécifique absorbée (J/kg).Dans un premier temps, des essais quasi-statiques (v = 5 mm/min) et dynamiques (v = 2 m/s) de compression uni-axiale sont conduits à température ambiante sur une seule sphère creuse de diamètre 30 mm. Une propagation rapide de fissures macroscopiques est observée. Le formalisme de la Mécanique Élastique Linéaire de la Rupture (MELR) est utilisé pour estimer le taux de restitution d’énergie critique dynamique GIdc du matériau constitutif. La position du sommet de fissure est mesurée pendant la propagation de fissure à l’aide d’une caméra rapide. La Méthode des Éléments Discrets (DEM) permet de simuler la rupture dynamique en implémentant une technique de relâchement des nœuds. Le taux de restitution d’énergie GIdc peut être estimé à partir de l’histoire (position et temps) du sommet de fissure. Le modèle numérique montre que les structures sphériques dissipent une proportion importante de l’énergie par des effets dynamiques. A une même vitesse de propagation, plus l’épaisseur de coque est fine, plus les effets inertiels générés par la rupture sont importants et ce pour une même vitesse de propagation.Le modèle numérique DEM est ensuite employé pour reproduire la rupture dynamique sur une sphère creuse à l’aide d’un critère en contrainte seule ou un critère mixte en contrainte – énergie. Les bons résultats obtenus démontrent la capacité de la DEM à représenter la propagation de fissures en régime dynamique.Finalement, des essais numériques et expérimentaux multi-sphères sont réalisés afin évaluer le comportement des sphères creuses au sein d’un assemblage. / Hollow sphere structure (HSS) belongs to cellular solids that have been studied recently for its multiples properties. In our case, HSS aims to absorb soft impacts energy on an airliner cockpit. HSS is investigated through the SAMBA (Shock Absorber Material for Bird-shield Application) project because of its promises in term of specific energy dissipated (J/kg) during impact.First of all, quasi-static and dynamic (v = 5 mm/min to v = 2 m/s) uniaxial compression tests are conducted at room temperature on a single sphere (D = 30 mm). Rapid crack propagation (RCP) is observed to be predominant at macroscopic scale. The formalism of Linear Elastic Fracture Mechanics (L.E.F.M.) is therefore used to estimate the dynamic energy release rate GIdc . The crack tip location is measured during the crack propagation using a high speed camera. The Discrete Element Method (DEM) is used to simulate the dynamic fracture by implementing the node release technique. The dynamic energy release rate can be determined using an experimentally measured crack history. In spherical structures the numerical results reveal a high proportion of energy dissipated through inertial effects as well as a dependence of the thickness of the hollow sphere over the range of 0.04 mm to 1.2 mm.The DEM model Is then employed to reproduce the RCP according to two failure criterions: a stress criterion and a coupled stress-energy criterion. It reveals to be an interesting way to model the mechanical behavior of brittle materials.Eventually, experimental and numerical multi-spheres tests are performed to evaluate the behavior of brittle hollow spheres within an assembly.

O câncer é um problema de saúde pública mundial, apresentando acréscimo na sua incidência a cada ano. O seu processo de evolução ainda não foi completamente desvendado, dificultando a elaboração de terapias adequadas. Na busca por um melhor prognóstico, pesquisas recentes têm discutido o papel das citocinas inflamatórias, do nicho perivascular e das células-tronco nos mecanismos de desenvolvimento e manutenção dos tumores malignos. Os tumores de glândula salivar representam uma pequena porcentagem das patologias malignas da região de cabeça e pescoço, podendo ocorrer em adultos e em crianças. O diagnóstico dificilmente é precoce e a taxa de sobrevida é extremamente baixa comparada aos demais tumores da região. Assim, este estudo teve como objetivo estudar as células provenientes dos tumores de glândula salivar do tipo adenoide cístico (CAC) e adenocarcinoma NOS (AdNOS) quanto ao seu perfil imunofenotípico, quanto à existência ou não de células-tronco tumorais nessa população, bem como investigar possíveis modificações na ativação de STAT3, Akt e ERK (moléculas envolvidas em vias de sinalização de manutenção do tumor), quando em contato com fatores secretados por células endoteliais. Foram coletados 5 CACs e 4 AdNOS, no Hospital da Universidade de Michigan (Ann Arbor, MI, EUA), durante 2010 e 2012. As células foram isoladas e caracterizadas em citometria de fluxo em P0 e P7, demonstrando um perfil de células CD44+ALDH+Lin- variando de 0,33 a 3,19% e 0,36 a 2,00%, respectivamente, entre 5 linhagens avaliadas. Na avaliação por western blotting, a e-caderina, o Snail e a actina de músculo liso foram ausentes em todos os tipos tumorais, enquanto que a citoqueratina 20 (Ck20) foi presente apenas nos AdNOS. Comparando os tumores com suas metástases, a presença de Ck20, p63 e β-catenina foi semelhante, enquanto que citoqueratina 7, a vimentina e o Bmi-1 foram maiores nas metástases. Tanto os AdNOS quanto CACs apresentaram receptores para IL-6, IL-8 e EGF. Foi observado que mediadores solúveis liberados pelas células endoteliais foram capazes de fosforilar STAT3, Akt e ERK em todas as células salivares estudadas, no entanto, a proteína recombinante humana IL-6, isoladamente, não foi capaz de ativar Akt. Orosferas foram geradas em todos os tipos tumorais, demonstrando o potencial de auto-renovação celular. Um maior número de esferas foi observado nas células metastáticas em relação às primárias. Células CD44+ALDH+, comparadas com CD44-ALDH-, geraram mais esferas, quando plaqueadas em alta densidade (5.000 células). No entanto, o inverso foi encontrado, quando uma única célula foi utilizada para o ensaio (p>0,05). Devido à dificuldade de obtenção e manipulação de células de tumores de glândula salivar, ainda há muito que se investigar mecanisticamente. Considerando a fosforilação de STAT3 na presença de IL-6, semelhante ao verificado em outros tumores, o uso de anticorpos contra IL-6, talvez sejam uma opção no futuro. / Cancer is a public health problem worldwide, with an increase in incidence every year. The process of its evolution is still not completely understood, hindering the development of appropriate therapies. In the search for a better prognosis, recent reports have discussed the role of inflammatory cytokines, perivascular niche and stem cells in the mechanisms of development and maintenance of malignant tumors. The salivary gland tumors represent a small percentage of malignancies of the head and neck and can occur in both adults and children. Early diagnosis is difficult and the survival rate is extremely low compared to other tumors in the same region. Thus, this study aimed to study cells from the adenoid cystic carcinoma (ACC) and adenocarcinoma NOS (AdNOS) tumors of salivary gland regarding its immunophenotypic profile and the existence or absence of tumor stem cells in this population, as well as investigate possible changes in the activation of STAT3, Akt and ERK (molecules involved in signaling pathways of tumor maintenance), when exposed to factors secreted by endothelial cells. ACCs (n=5) and AdNOS (n=4) were collected at the Hospital of the University of Michigan (Ann Arbor, MI, USA), during 2010 to 2012. Cells were isolated and characterized by flow cytometry at P0 and P7, showing a profile of ALDH+CD44+Lin- ranging from 0.33% to 3.19% and 0.36% and 2.00%, respectively, between 5 cell lines evaluated. In the protein profile, e-cadherin, Snail and SMA were absent in all tumor types. Ck20 was present only in AdNOS. Comparing primary tumors and their metastases, the presence of Ck20, and p63 β-catenin was similar, while Ck7, vimentin and Bmi-1 were higher in metastases. Both AdNOS as ACCs had receptors for IL-6, IL-8 and EGF. It was observed that soluble mediators released by endothelial cells were able to activate STAT3, Akt and ERK phosphorylation in all cells studied. However, recombinant human IL-6 alone was not able to activate Akt. Orospheres were generated in all tumor types, indicating the potential for cellular self-renewal. Highest number of spheres was observed in metastatic cells compared to primary. ALDH+CD44+ cells compared to ALDH-CD44- generated more spheres when plated in high density (5,000 cells), however, the opposite was found when one single cell seed was evaluated (p> 0.05). There is doubt if these cell markers would be consider for a stem cell model in salivary tumors. Due to the difficulty of obtaining and manipulating salivary gland tumor cells, there is still much to investigate mechanistically. As the phosphorylation of STAT3, in the presence of IL-6, was similar to that observed in other tumors, the use of antibodies against IL-6, may be an option in the future.

A presente tese, de natureza analítica, defende, a partir da análise dos elementos subjacentes à ideia de direito como sistema por trás da discussão sobre a fragmentação do direito internacional, que a expansão do direito internacional no século XX foi acompanhada de uma mudança do conceito de legitimidade, que não se restringe mais apenas ao reconhecimento do Estado como produtor das normas internacionais, mas requer parâmetros substantivos que orientam a solução dos conflitos entre normas que são produzidas em diferentes esferas normativas. No entanto, ao contrário do que ocorre no ordenamento interno, cujo sistema jurídico estabelece uma hierarquia de normas neutralizada pela ideologia do regime existente, nas relações internacionais, a horizontalidade das normas do direito internacional reflete a inexistência de uma cadeia tal de valores; e nem mesmo a proliferação de normas secundárias na segunda metade do século XX logrou alterar os fundamentos da jurisdição internacional, a qual ainda encontra diversas restrições associadas às características do direito internacional de coexistência. Estes problemas serão evidenciados pela análise do que ocorre no âmbito da OMC e, dentre as diversas disputas que envolvem normas produzidas em diferentes esferas normativas, do caso Brazil Measures Affecting Imports of Retreaded Tyres (União Européia v. Brasil) [doravante Brazil Retreaded Tyres]. / From analysis of the factors underlying the idea of law as a system behind the discussion of the \"fragmentation\" of international law, this thesis, analytical in nature, argues, that the expansion of international law in the twentieth century was accompanied by a change in the concept of legitimacy, which is no longer restricted only to the State as a producer of international standards, but requires substantive parameters to guide the resolution of conflicts between rules from different normative spheres. However, unlike domestic domains, where the legal system establishes a hierarchy of norms neutralized by the ideology of the existing regime, in international relations, the horizontality of the norms of international law reflects the absence of such a chain of values, nor even the proliferation of secondary standards in the second half of the twentieth century managed to change the fundamentals of international jurisdiction, which still faces several constraints associated with the characteristics of the international law of coexistence. These problems will be highlighted by the analysis of what occurs in the WTO and, among the several disputes involving standards from different normative spheres, the case of Brazil Measures Affecting Imports of retreaded Tyres (European Union v. Brazil) [hereinafter Brazil - retreaded Tyres].

Made available in DSpace on 2015-04-22T22:16:13Z (GMT). No. of bitstreams: 1 Francisco Eteval da Silva Feitosa.pdf: 212887 bytes, checksum: e8aec2d3b5fcc99646bc7f05c844ccd8 (MD5) Previous issue date: 2003-01-22 / The purpose of this essay is to make a clear and detailed exposition of the work of Robert L. Bryant on the class of Weingarten Surfaces immersed in the Euclidean tree-space, E3, that satisfy the equation (formula), where H and K denote the mean and the Gaussian curvatures respectively and f is a smooth function. The surfaces with constant mean curvature and those with constant Gaussian curvature clearly belong to this class and if they have genus zero, the Hopf`s and the Liebermann`s theorems respectively state that they are standard spheres of E3. The main result of this work characterizes the standard spheres as the only Wein-garten surface of genus zero in the mentioned class. / Esta dissertação tem como finalidade apresentar uma exposição clara e detalhada de um trabalho de Robert L. Bryant intitulado Análise Complexa e uma Classe de Superfícies de Weingarten, superfícies essas, imersas em E3, que satisfazem a equação (formula) onde H e K são as curvaturas média e gaussiana, respectivamente, e f é uma função real diferenciável. As superfícies com curvaturas média e gaussiana constante, pertencem claramente a esta classe e se elas têm gênero zero os teoremas de Hopf e de Liebermann, respectivamente, asseguram que elas são esferas usuais de E3. O principal resultado deste trabalho caracteriza as esferas usuais como as únicas superfícies de Weingarten de gênero zero pertencentes à classe mencionada.

Submitted by Silvana Teresinha Dornelles Studzinski (sstudzinski) on 2016-12-20T15:48:37Z No. of bitstreams: 1 Fábio Faistauer_.pdf: 2685236 bytes, checksum: 4421428b568b50c93c4b5bc55df13256 (MD5) / Made available in DSpace on 2016-12-20T15:48:37Z (GMT). No. of bitstreams: 1 Fábio Faistauer_.pdf: 2685236 bytes, checksum: 4421428b568b50c93c4b5bc55df13256 (MD5) Previous issue date: 2016-08-16 / FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul / Este trabalho apresenta um estudo numérico do processo de mudança de fase de PCM (Phase Change Material ou Materiais de Troca de Fase) acondicionados em cavidades esféricas. O modelo numérico é bidimensional e composto pelas equações de conservação da massa, da quantidade de movimento, de energia e da fração volumétrica, modeladas através da técnica entalpia-porosidade. A malha computacional é do tipo hexaédrica com refinamentos nas regiões que apresentam os maiores gradientes térmicos e fluidodinâmicos. O modelo numérico foi validado com resultados experimentais e numéricos da literatura. São estudados casos de fusão de PCM RT 35, RT 55 e RT 82 em cavidade esférica com temperatura constante na parede. Os diâmetros das esferas são de 40, 60, 80 e 100 mm e as temperaturas impostas à parede são de 10, 20 e 30°C acima da temperatura de fusão dos PCM. São apresentados resultados de fração líquida e de fluxo de calor resultante dos 36 casos estudados. A pesquisa mostra que PCM com propriedades semelhantes apresentam o mesmo comportamento para fração líquida e fluxo de calor, independentemente de seu ponto de fusão. O aumento da temperatura na parede da esfera é responsável por maiores fluxos de calor e pela diminuição do tempo de obtenção da fração líquida. Comparando as diferenças de temperaturas de 10, 20 e 30°C, a melhor redução percentual do tempo de fusão foi obtida para a temperatura de 20°C. A pesquisa também mostra que o aumento do diâmetro não influencia o fluxo de calor inicial, porém aumentam o tempo de fusão do PCM. Este tempo de fusão pode ser relacionado com o comprimento característico da esfera, independente das temperaturas impostas à parede. Os estudos mostram também que a maior quantidade de armazenamento térmico através do PCM líquido é obtida com a combinação dos maiores diâmetros com as maiores temperaturas. Para obter o cálculo da fração líquida em função do tempo através de uma correlação, os parâmetros envolvidos foram calculados através dos números de Fourier, Stefan, Grashof e Prandtl. / This paper presents a numerical study of the phenomena that occur in the use of PCM (Phase Change Materials) packed in spherical cavities. The numerical simulation was performed with commercial software ANSYS-FLUENT. The numerical model is two-dimensional and consists of the mass conservation equations of momentum, energy and volume fraction, modeled by enthalpy-porosity technique. The computational mesh is the hexahedral type with refinements in regions with the highest thermal and fluid dynamic gradients. The numerical model was validated with experimental and numerical results of literature. It was studied melting cases of PCM RT 35, RT 55 and RT 82 in spherical cavities with constant temperature on the wall. The diameters of the spheres were 40, 60, 80 and 100 mm and temperatures imposed on the wall were 10, 20 and 30°C, above the melting temperature of the PCM. It was presented results of melt fraction and heat flow of 36 cases studied. Research shows that PCM with similar properties have the same behavior for melt fraction and heat flow, regardless of its melting temperature. The temperature rise in the sphere wall is responsible for higher heat flows and by decreasing the melt fraction obtained in time. The best percentage reduction in the melting time was obtained with 20°C of temperature differences. Research also shows that the increase in diameter does not influence the heat flux, but increases the PCM melting time. This melting time can be related to the sphere characteristic length, regardless of the temperatures imposed on the wall. The studies also show that the largest quantity of thermal storage through the liquid PCM is obtained by combining the larger diameters with higher temperatures. The main parameters involved in the phase change process are correlated through numbers of Fourier, Stefan, Grashof and Prandtl for the calculation of the liquid fraction as a function of time. To calculate the liquid fraction as a function of time through a correlation, the parameters involved were calculated using the Fourier, Stefan, Grashof and Prandtl numbers.

Esta tese tem por objetivo explorar os mecanismos e dinâmicas que podem afetar as trajetórias educacionais dos jovens, provocando mudanças ou fortalecendo a sua direção inicial. Para caracterizar o tipo de trajetória, olhamos para os seguintes elementos: distorção idade-série, reprovações, expulsões e evasão escolar. Exploramos de que forma as esferas de sociabilidade em que os jovens estão inseridos - a família, a escola, a vizinhança, o trabalho, os programas sociais, a igreja e o lazer se articulam e afetam as suas trajetórias escolares. Além disso, investigamos em que medida eventos que ocorrem ao longo da vida do jovem podem afetar a sua conduta, a sua relação com os elos estabelecidos nos diferentes espaços de sociabilidade e, consequentemente, os seus percursos educacionais. As análises feitas neste trabalho são de cunho qualitativo. Utilizamos os seguintes instrumentos: entrevistas em profundidade, guiadas por um roteiro prévio, conversas livres, observação participativa e observação passiva da interação. Apesar de termos nos limitado a uma escola, encontramos entre os entrevistados heterogeneidade em termos de atributos individuais e dimensões socioeconômicas e culturais, fatores que influenciam as trajetórias escolares. No entanto, identificamos jovens com atributos e condições socioeconômicas similares, mas trajetórias distintas. Nestes casos, as relações estabelecidas com os elos das esferas de sociabilidade e as formas como lidaram com as situações de crise explicam as diferenças encontradas. / This thesis aims to explore the mechanisms and dynamics that may affect, cause changes or strengthen the initial direction of the educacional trajectories of youngsters. In order to characterize the type of trajectory, we looked at the following factors: age-grade distortion, grade retention, expulsion and drop-out. We explored how the spheres of sociability of the youngsters - family, school, neighbourhood, work, social programs, church and leisure - are associated and affect their school trajectories. In addition, we investigated the extent to which events that occur throughout their life can affect their behaviour, their relationship with ties established in different spheres of sociability and, consequently, their educational trajectories. The analysis made in this study are qualitative. The following instruments were used: in-depth interviews, free conversations, participant observation and passive interaction observation. Although we limited ourselves to one school, we found heterogeneity among respondents in terms of individual attributes and socioeconomic and cultural dimensions, factors that influence school trajectories. However, we identified young people with similar socioeconomic characteristics and conditions but distinct paths. In these cases, the differences found can be explained by the relationship established with ties in spheres of sociability and the ways in which crisis situations were dealt with

This study explores cultural productions in one television genre in Chinese mainstream media: dispute resolution shows. By applying the theoretical frameworks of Hall’s encoding and decoding and Habermas’ public spheres, this study mainly answers two research questions: 1) how does mainstream production convey politically-preferred cultural and social values to viewers; and 2) how do audience members exercise their citizenship in decoding televised social values and cultural norms? In a specific examination of Oriental Pearl Live Newsroom, mixed-methods are adopted, including unsupervised learning of the Latent Dirichlet Allocation (LDA), content analysis, thematic analysis, focus groups, and interviews. In the findings, the interviewees admitted that they propagated social and cultural values in accordance with the mainstream political ideology of the Chinese Communist Party (CCP) in order to maintain social stability and state order. However, my interviewees also suggested that they had an ambition and willingness to promote a civil society in China, which requires a counterbalanced power from the audience’s side. The results of the audience analysis generally indicated that they challenged the power of legitimate authorities, including the nation-state, the elite class, and the media. This study identified five online public spheres: 1) Government is the core; 2) Request for rule of law; 3) Media is a paradox; 4) The elite class is not the boss; 5) The grass is always greener (adoration of foreign countries). In general, this study supports conceptualizing audience members as citizens. It demonstrates how audience members deconstruct the dominant interpretations of social values and their attempts to elaborate less-favorable voices in Transitional Chinese society. This conceptualization suggests the importance of audience members in creating diverse public spheres and promoting a civil society.

My dissertation, Understanding the Gray:Aging Women in Victorian Culture and Fiction, explores the cultural construction of aging for middle-class Victorian women and how aging was experienced and then depicted within novels. Chiefly, I work from midcentury to the end of the century in order to understand the experience of aging and ways women were ascribed age due to their position in society as spinsters, mothers, and progressive women. I explore how the age of fictional women reflects and contributes to critical debates concerning how Victorian women were expected to behave. Debates over separate spheres, how women were perceived in British society, and how women’s rights changed during the 19th century highlight how aging affected women and how they were treated throughout the century. Victorian fiction illustrates the ways women achieved different roles in society and how age and the perception of age affected their ability to do so. Understanding how aging was experienced, understood, and ascribed to Victorian women who fought in various ways for new terms of citizenship and mobility helps us begin to trace how we treat and respond to aging in women today. The first chapter outlines the social status of unmarried women and spinsters, considering how age affected women’s ability to lead professional lives in Charlotte Brontë’s Villette (1853). The second chapter, on George Eliot’s Felix Holt: The Radical, explores older motherhood through Mrs Transome and illustrates how the novel seeks to teach younger women of the pitfalls of unequal marriages. The third chapter builds a cultural understanding of how aging was linked to progressive, anti-domestic womanhood and racial impurity through the New Woman and in H.R. Haggard’s She.

Much of the aerosol research completed at the University of South Florida has revolved around evaluating industrial hygiene equipment and instrumentation in environmental chambers. Data collected during these studies has provided valuable baseline data on equipment and instrument performance under calm air conditions. A newly constructed wind tunnel now allows researchers to evaluate industrial hygiene instruments under moving air conditions. Because the wind tunnel is capable of producing wind velocities that a worker could encounter in the occupational setting, researchers may gain insight into instrument performance under simulated field conditions. Because aerosols can be introduced into the new wind tunnel testing section, researchers can also challenge industrial hygiene equipment and instrumentation with aerosols in sizes ranges that are of interest in public health. The purpose of this dissertation research was to develop a new wind tunnel to be used for aerosol research at the University of South Florida. Three specific aims had to be met for this study to be successful. They included: (1) designing a wind tunnel based on best practice information outlined in scientific literature, (2) constructing an operable wind tunnel to be used for aerosol research, and (3) characterizing wind tunnel performance by examining the wind tunnel velocity profile, turbulence intensity, and aerosol introduction/collection. The actual wind tunnel was constructed to a length of approximately 20 feet, a height of approximately 2 feet at its tallest point, and includes an entrance filter housing, a settling chamber, a contraction, a testing section, a diffuser, an exit filter housing, a fan, and exhaust duct. All components were designed and constructed using guidelines and best practices reported in the scientific literature. Velocity profile measurements were the first way that this wind tunnel was characterized. In order to successfully obtain measurements, the wind tunnel cross section was divided into 16 equal quadrants. Five measurements were taken for each quadrant at each wind velocity. Target wind velocities for this research were 0.5 m/s, 1.0 m/s, and 2.0 m/s. Actual average wind velocities of 0.48 m/s, 1.00 m/s, and 2.04 m/s. All were within established limits reported in the scientific literature. Turbulence intensity measurements were the second way that this wind tunnel was characterized. In order to successfully obtain measurements, the wind tunnel cross section was divided into 16 equal quadrants. Five measurements were taken for each quadrant at each wind velocity. Wind tunnels are typically designed to have the lowest turbulence intensity possible, generally below 10%. The overall average turbulence intensities for this wind tunnel at wind velocities of 0.5 m/s, 1.0 m/s, and 2.0 m/s were 9%, 10%, and 8% respectively. Overall turbulence intensity measurements were at or below 10%. Isokinetic sampling was the final method used to characterize this wind tunnel by collecting and detecting aerosols traveling through the wind tunnel testing section. The wind tunnel was operated at wind velocities of 0.5 m/s, 1.0 m/s, and 2.0 m/s with isokinetic sampling flow rates of 15.4 L/min, 30.9 L/min, and 61.7 L/min respectively. Monodisperse fluorescent polystyrene latex spheres were used as the test aerosol because they are uniform in size and shape and can be detected by fluorometry. The Blaustein Multi-Jet Atomizer (BLAM) was used to generate monodisperse fluorescent polystyrene latex aerosol 0.5 µm and 2.0 µm particles from liquid suspensions. The Vilnius Aerosol Generator (VAG) was used to generate monodisperse fluorescent polystyrene latex aerosol of 6.0 µm and 12.0 µm particles from dry powders. Nitrogen gas was used for delivering test aerosols into the wind tunnel. Five experimental runs were completed for each particle size and wind velocity for a total of 60 experimental runs. Fluorescence was detected in all 60 samples with average mass concentrations ranging from 0.000050 ng/ml to 0.002703 ng/ml. Based on velocity profile measurements, turbulence intensity measurements, and isokinetic sampling, the performance of University of South Florida wind tunnel was found to be excellent, indicating that it was designed and constructed appropriately. The wind tunnel can now successfully be used by researchers interested in evaluating industrial hygiene sampling equipment with aerosols ranging from 0.5 µm to 12.0 µm in moving air with velocities ranging from 0.5 m/s to 2.0 m/s.