Law Wing Cheung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references. / Abstracts in English and Chinese. / Abstract --- p.I / Acknowledgements --- p.V / List of Publications related to this project --- p.VI / Contents --- p.VII / Chapter Chapter 1 --- Introduction --- p.1-1 / Chapter Chapter 2 --- Literature Review / Chapter 2.1 --- Surface Plasmon Waves --- p.2-2 / Chapter 2.2 --- Excitation of Surface Plasmon --- p.2-4 / Chapter 2.2.1 --- Surface Plasmon Coupling Schemes --- p.2-6 / Chapter 2.3 --- Detection Techniques used in SPR sensors --- p.2-13 / Chapter 2.3.1 --- Angular Interrogation --- p.2-14 / Chapter 2.3.2 --- Wavelength Interrogation --- p.2-15 / Chapter 2.3.3 --- Intensity Interrogation --- p.2-16 / Chapter 2.3.4 --- Phase Interrogation --- p.2-16 / Chapter 2.3.5 --- Commercial SPR biosensors --- p.2-18 / Chapter 2.3.6 --- Comparison between Detection Techniques --- p.2-19 / Chapter 2.4 --- Applications of SPR biosensors --- p.2-21 / Chapter Chapter 3 --- Principle of Surface Plasmon Resonance Sensing Technology / Chapter 3.1 --- SPR Phenomenon --- p.3-1 / Chapter 3.2 --- Conditions for Surface Plasmon Resonance --- p.3-5 / Chapter 3.3 --- Wave-vectors --- p.3-7 / Chapter 3.4 --- Surface Plasmon Resonance described by Fresnel's Theory --- p.3-8 / Chapter 3.5 --- Concept of Surface Plasmon Resonance Biosensing --- p.3-10 / Chapter Chapter 4 --- Experiments / Chapter 4.1 --- Highly sensitive differential phase-sensitive surface plasmon resonance biosensor based on Mach-Zehnder configuration --- p.4-1 / Chapter 4.1.1 --- Materials required --- p.4-1 / Chapter 4.1.2 --- Experimental Setup --- p.4-2 / Chapter 4.1.3 --- Principle of Differential Phase Measurement --- p.4-3 / Chapter 4.1.4 --- Photodetector Circuitry --- p.4-6 / Chapter 4.1.5 --- Digital Signal Processing --- p.4-7 / Chapter 4.1.6 --- Polymer based Micro-fluidic System Integrated with SPR Biosensor --- p.4-9 / Chapter 4.2 --- Phase-sensitive Surface Plasmon Resonance Biosensor using the Photoelastic Modulation Technique --- p.4-12 / Chapter 4.2.1 --- Materials required --- p.4-12 / Chapter 4.2.2 --- Experimental Setup --- p.4-13 / Chapter 4.2.3 --- Principle of Photoelastic Modulation Technique and Signal Processing --- p.4-14 / Chapter 4.2.4 --- Operation Principle of Photoelastic Modulator --- p.4-17 / Chapter 4.3 --- Sample Preparations --- p.4-18 / Chapter 4.3.1 --- Glycerin-water Mixtures --- p.4-18 / Chapter 4.3.2 --- "PBS, BSA and BSA antibody" --- p.4-19 / Chapter 4.3.3 --- "RPMI, Trypsin, Cells and SDS" --- p.4-20 / Chapter Chapter5 --- Results amd Discussions / Chapter 5.1 --- Experimental setup I: Highly sensitive differential phase-sensitive surface plasmon resonance biosensor based on Mach-Zehnder configuration --- p.5-1 / Chapter 5.1.1 --- Measuring various glycerin-water concentration mixture with silver-gold sensing layer --- p.5-1 / Chapter 5.1.2 --- Comparison between the sensitivity of our setup and reported setup based on phase detection --- p.5-4 / Chapter 5.1.3 --- Discussion on 0.01° system resolution --- p.5-7 / Chapter 5.1.4 --- Experiment on monitoring BSA-BSA antibody binding reaction --- p.5-9 / Chapter 5.1.5 --- Matching oil and glass slide --- p.5-11 / Chapter 5.1.6 --- Experiments on monitoring BSA-BSA antibody binding reaction with integrated microfluidic system --- p.5-12 / Chapter 5.1.7 --- Experiment on observing cell adhesion properties on gold surface under the influence of trypsin --- p.5-14 / Chapter 5.1.8 --- Discussion on the non-specific binding between trypsin and gold surface --- p.5-16 / Chapter 5.1.9 --- Modifying the gold surface with BSA layer --- p.5-17 / Chapter 5.1.10 --- Experiment on observing cell adhesion properties on the gold surface under the influence Sodium Dodecyl Sulfate (SDS) --- p.5-18 / Chapter 5.2 --- Experimental setup II: Phase-sensitive surface plasmon resonance biosensor using the photoelastic modulation technique --- p.5-21 / Chapter 5.2.1 --- Measurement on difference glycerin-water concentration mixture --- p.5-21 / Chapter 5.2.2 --- Experiment on monitoring BSA-BSA antibody binding reaction --- p.5-23 / Chapter Chapter 6 --- Conclusions and Future Works / Chapter 6.1 --- Conclusions --- p.6-1 / Chapter 6.2 --- Future Works --- p.6-2 / References --- p.R-1 / Appendix / Chapter A. --- Phase Extraction Routine written by Matlab --- p.A-1 / Chapter B. --- Mathematical expressions for calculating the phase angle in the experiment of SPR biosensor using the Photoelastic Modulation Technique --- p.A-6 / Chapter C. --- Relationship between Concentration and Refractive Index of Glycerin-Water Mixture --- p.A-11 / Chapter D. --- Physical Properties of Bovine Serum Albumin --- p.A-12 / Chapter E. --- Simulation Curve written by Matlab --- p.A-13