Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 112-118).

Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 112-118).

No description available.

Thesis (M.Nurs.)--University of Hong Kong, 2008. / Includes bibliographical references (p. 59-65)

published_or_final_version / Nursing Studies / Master / Master of Nursing

Measurements of mean velocity and mean temperature fields and wall parameters for air flowing in a smooth, vertical tube at low entry Reynolds numbers are presented for heating with constant wall heat flux along the heated length. Two entry Reynolds numbers of approximately 6000 and 4000 were employed with three heating rates, q('+) = q('w'')/ (Gc(,p,i) T(,i)), of approximately 0.0018, 0.0035 and 0.0045. The flow development was measured by obtaining internal profiles along the heated length at axial locations from x/D = 3.17 to x/D = 24.54. An adiabatic entry of 50 diameters preceded the heated region. The three heating rates caused slight, large and severe property variation of the air. The highest heating rate was found to cause significant buoyancy effects. The internal measurements were obtained using constant temperature hot-wire anemometry and resistance thermometry for velocity and temperature, respectively, employing a single short wire probe. The technique developed and employed for the use of a single short hot wire in velocity measurements in non-isothermal flows is presented. The measurements are compared to numerical predictions employing two simple versions of the van Driest mixing length turbulence model. In general, both models agreed with the measurements reasonably well, but for the higher heating rates neither model was completely satisfactory in predicting the velocity profiles. When the buoyancy parameter reached 0.3, the peak velocity occurred in the wall region rather than at the tube centerline. Typically, the Nusselt number was overpredicted by 10% for x/D > 14 and, consequently, the wall temperature was underpredicted by about 7%.

Not available / text

The ability to rapidly, sensitively, and accurately detect the presence of a pathogen is a vital capability for first responders in the assessment and treatment of scenarios such as disease outbreak and bioterrorism. Nucleic acid tests such as the polymerase chain reaction (PCR) are supplanting traditional techniques due to the improved speed, specificity, sensitivity, and simplicity. Still, amplification by PCR is often the bottleneck when processing genetic samples. Conventional PCR machines are bulky, slow, and consume large reagent volumes and an affordable, compact, efficient, easy-to-use alternative has yet to emerge. In this work, a microfluidic PCR platform was developed consisting of a low-cost, multi-chamber polymer microchip and a laser-mediated thermocycler capable of independent thermal control of each reaction chamber. Innovations in polymer microchip modeling, fabrication, and characterization yielded a low-cost solution for sample handling. A simple optical system featuring an infrared laser diode and solenoid-driven optical shutter was combined with a microfluidic temperature measurement system utilizing embedded thermocouples to achieve rapid thermocycling capable of multiplexed temperature control. We validated the instrument with sensitive amplifications of multiple viral targets simultaneously. This technology is a breakthrough in practical microfluidic PCR instrumentation, providing the foundation for a paradigm shift in low-cost, high-throughput genetic diagnostics.

Thesis (Ph. D.)--University of Texas at Austin, 2005. / Supervisor: Thomas F. Edgar. Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.